Induction of apoptosis in bone marrow cells by gangliosides produced by a T cell lymphoma.

Abstract:

:The present study was conducted to investigate the mode of cell death of bone marrow cells (BMC) treated in vivo or in vitro with gangliosides (DLG) derived from a T cell lymphoma, designated as Dalton's lymphoma (DL). BMC undergoing cell death showed morphological features characteristic to apoptosis such as cell shrinkage, plasma membrane blebbing and densely stained chromatin. DLG-induced apoptosis was further confirmed by analysis of DNA from DLG-treated BMC by flow cytometry and agarose gel electrophoresis which showed an increase in hypodiploid DNA and internucleosomal DNA cleavage, respectively. Immunoblotting of p53 and Bax demonstrated an increase in the levels of p53 and Bax proteins. BMC treated with DLG also showed translocation of NF-kappaB protein into the nucleus. Antibodies to ganglioside G(D3), a constituent of DLG, neutralized the effect of DLG. Further, supplementation of DLG-containing BMC cultures with granulocyte macrophage-colony stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) prevented the apoptosis-inducing action of DLG.

journal_name

Immunol Lett

journal_title

Immunology letters

authors

Bharti AC,Singh SM

doi

10.1016/s0165-2478(00)00156-5

subject

Has Abstract

pub_date

2000-04-03 00:00:00

pages

39-48

issue

1

eissn

0165-2478

issn

1879-0542

pii

S0165-2478(00)00156-5

journal_volume

72

pub_type

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