Abstract:
:The present study was conducted to investigate the mode of cell death of bone marrow cells (BMC) treated in vivo or in vitro with gangliosides (DLG) derived from a T cell lymphoma, designated as Dalton's lymphoma (DL). BMC undergoing cell death showed morphological features characteristic to apoptosis such as cell shrinkage, plasma membrane blebbing and densely stained chromatin. DLG-induced apoptosis was further confirmed by analysis of DNA from DLG-treated BMC by flow cytometry and agarose gel electrophoresis which showed an increase in hypodiploid DNA and internucleosomal DNA cleavage, respectively. Immunoblotting of p53 and Bax demonstrated an increase in the levels of p53 and Bax proteins. BMC treated with DLG also showed translocation of NF-kappaB protein into the nucleus. Antibodies to ganglioside G(D3), a constituent of DLG, neutralized the effect of DLG. Further, supplementation of DLG-containing BMC cultures with granulocyte macrophage-colony stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) prevented the apoptosis-inducing action of DLG.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Bharti AC,Singh SMdoi
10.1016/s0165-2478(00)00156-5subject
Has Abstractpub_date
2000-04-03 00:00:00pages
39-48issue
1eissn
0165-2478issn
1879-0542pii
S0165-2478(00)00156-5journal_volume
72pub_type
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