Abstract:
:The monoclonal antibody (mAb) AHT-107 recognized a determinant distal to the interleukin 2 (IL-2) binding site on the p55 subunit of the IL-2 receptor (IL-2R) (the Tac antigen, CD25) of human T lymphoblasts, while the mAb AHT-54 recognized a determinant close to the IL-2 binding site as did the anti-Tac. The AHT-107 inhibited IL-2 dependent proliferation of human T lymphoblasts equally as well as did the AHT-54. Both mAbs inhibited the high-affinity binding and crosslinking of IL-2 to the p55 + p75 heterodimeric complex on forskolin-treated YT cells. Remarkably, the AHT-107 did not inhibit the low-affinity binding and cross-linking of IL-2 to the p55 molecule on human p55 cDNA-transfected cells, while the AHT-54 as well as anti-Tac did so. In contrast, the mAb PC61, that was previously reported to recognize a determinant distal to the IL-2 binding site on the mouse p55 subunit of IL-2R and to dissociate IL-2 from the high-affinity IL-2R complex by altering the conformation of the p55 molecule itself, inhibited the low-affinity binding and cross-linking of IL-2 to the p55 molecule on mouse p55 cDNA-transfected cells. Further, we showed that the AHT-107 did not dissociate IL-2 from the high-affinity IL-2R complex once formed on human T lymphoblasts.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Osawa H,Diamantstein Tdoi
10.1016/0165-2478(89)90081-3subject
Has Abstractpub_date
1989-02-01 00:00:00pages
205-12issue
3eissn
0165-2478issn
1879-0542pii
0165-2478(89)90081-3journal_volume
20pub_type
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journal_title:Immunology letters
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更新日期:2006-03-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:2004-03-29 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2005-05-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.05.001
更新日期:2009-06-04 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2010.04.009
更新日期:2010-08-16 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00043-5
更新日期:1995-05-01 00:00:00
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pub_type: 历史文章,杂志文章,评审
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更新日期:2017-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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journal_title:Immunology letters
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更新日期:2014-11-01 00:00:00
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更新日期:1987-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1998-12-01 00:00:00
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更新日期:1986-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-08-30 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90195-8
更新日期:1989-09-01 00:00:00