Abstract:
BACKGROUND:CD3 and CD19 are the characteristic surface markers of mature T lymphocytes and B lymphocytes of human respectively. A special subset of immune cells that characteristically expressed the surface markers CD19+ of B lymphocytes and CD3+ of T lymphocytes simultaneously (CD19+CD3+ cells, hereinafter referred to as B-T cells) was found in the peripheral blood of human, yet it has not been reported in cancer research before. Our aims were to characterize the expression and possible value of B-T cells in cancer patients. METHODS:Flow cytometry was applied to analyse the CD19+CD3+ cells, and laser scanning confocal microscope was utilized to prove co-expressing CD19+ of B lymphocytes and CD3+ of T lymphocytes simultaneously on the surface of the cells. Then a total of 523 patients with malignant tumor were enrolled in this study, and 177 healthy donors were recruited as the control group. The levels of CD19+CD3+ cells in peripheral blood were measured by flow cytometry, and the differences between the two groups were compared. RESULTS:The healthy donors and cancer patients all had B-T cells in their peripheral blood, but the percentage of B-T cells was 0.16 % ± 0.11 % and 0.58 % ± 0.38 % respectively, showing statistically significant (P < 0.0001). There was no significant correlation between the percentage of B-T cells and lymphocyte subsets (P > 0.05). The percentages of B-T cells in different tumor species were different. The proportion of B-T cells was high in esophageal cancer, non-Hodgkin's lymphoma and lung cancer, but it was low in pancreatic cancer, ovarian cancer and kidney cancer. Meanwhile, there was significant difference between esophageal cancer and kidney cancer (P < 0.001). The distribution of B-T cells in pancreatic cancer and kidney cancer was more concentrated, yet more dispersed in other cancers. Although there was a trend of increase in clinical stage Ⅲ+Ⅳ and a trend of decrease in age above 60 years for breast cancer, gastric cancer and liver cancer, there was no significant difference in the percentage of B-T cells in age, gender, different clinical stages, tumor metastasis, lymph node metastasis, and splenomegaly (P > 0.05). CONCLUSION:The percentage of B-T cells in cancer patients was significantly higher than that of healthy donors. B-T cells maybe play a very complicated role in tumor, whether it could be a potential tumor immune marker or not and what are the specific phenotypes and functions of it to need be further verified.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Liu Y,Ye S,Guo X,Li W,Xia Y,Wen X,Yu J,Jia Y,Liu X,Guo Y,Zhao Ydoi
10.1016/j.imlet.2020.01.007subject
Has Abstractpub_date
2020-04-01 00:00:00pages
44-50eissn
0165-2478issn
1879-0542pii
S0165-2478(19)30546-2journal_volume
220pub_type
杂志文章abstract::Satratoxins have been recognized as potential immunomodulatory agents in outbreaks of building-related illness. Here we report that satratoxin G-treated human leukemia HL-60 cells underwent apoptosis through the action of caspase-3 which was activated by both caspase-8 and caspase-9. Western blot analysis of caspase-3...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00127-x
更新日期:2002-10-21 00:00:00
abstract::The immunomodulating properties of a neuropeptide hormone, corticotropin-releasing factor (CRF), led us to investigate its effect on cAMP production by human peripheral blood mononuclear cells (MNC). In response to stimulation with CRF (100 nM), a statistically significant (P = 0.019) increase occurred in the amount o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90189-9
更新日期:1993-03-01 00:00:00
abstract::To study the interactions between Chlamydia trachomatis (CT) and human immunodeficiency virus (HIV) infections, we examined CT serologies in sequential serum samples of male homosexuals (MHS), followed over a mean period of 4 years. Of the MHS studied, 77 were HIV(-), 18 were HIV(+) and 10 patients seroconverted durin...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90201-1
更新日期:1994-04-01 00:00:00
abstract::We have examined whether the interaction of peptide-loaded MHC molecules on the surface of B-cells with antigen-specific T-cell receptors (TCRs) enhances Ig secretion in the presence of other antigen-independent interactions in vitro. B-cells specific for region 25-40 of beta-lactoglobulin (beta-LG) were stimulated in...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00002-9
更新日期:1998-04-01 00:00:00
abstract::Coenzyme Q10 (CoQ10) is a lipid-soluble antioxidant synthesized in human body. This enzyme promotes immune system function and can be used as a dietary supplement. Rheumatoid arthritis (RA) is an autoimmune disease leading to chronic joint inflammation. RA results in severe destruction of cartilage and disability. Thi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2015.05.012
更新日期:2015-08-01 00:00:00
abstract::Natural killer (NK) cells and cytotoxic T lymphocytes (CTL), the functional coordination of which are governed by various signal substances, are crucial in the body's defense of tumor and virus-infected cells. We investigated the role of various neurotransmitters and hormones on the regulation of functional parameters...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2003.09.004
更新日期:2003-12-15 00:00:00
abstract::Corticotropin releasing hormone (CRH) is a major regulator of the stress response. This study examined whether CRH regulates interleukin-18 expression on microglia, BV2. Our data show that CRH enhanced IL-18 expression and significantly induced the secretion of functional IL-18 protein. Furthermore, CRH induced IL-18 ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.12.003
更新日期:2005-05-15 00:00:00
abstract::A considerable body of evidence from various laboratories indicates that specific T cell responses generated during infection with Leishmania parasites play an important role both in the resolution and progression of cutaneous leishmaniasis. Recent data, summarized in this article, indicate that resolution of lesions ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90151-4
更新日期:1988-11-01 00:00:00
abstract::SJL mice develop resistance against tolerance between the 9th and 25th wk of life. This resistance is linked with a loss of suppressor capacity in the thymus. We have shown here that contact photosensitivity (CPS) decreases as a function of age and that this is due to an age-dependent increase in suppressor capacity. ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90102-1
更新日期:1986-03-01 00:00:00
abstract::We have previously reported the production of a series of human monoclonal antibodies reacting with mouse mammary tumor viral antigens as well as the human counterpart HuMTV antigen. Against two of these antibodies (B11 and 4.6/6), anti-idiotypic antibodies were generated, which appeared to be the internal image of vi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90128-w
更新日期:1991-04-01 00:00:00
abstract::Both CD8 and CD2 are T cell surface receptors involved in physical cell interaction and in transmembrane signalling. The present paper addresses their role in the induction of two different functions of the cloned murine cytotoxic T cell C196: target cell lysis and IFN-gamma production. These functions were induced in...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90018-6
更新日期:1991-10-01 00:00:00
abstract::In a previous study we have shown that, in response to electrical stimulation, there is a substantial release of noradrenaline (NA) from the sympathetic nerve terminals of the rat thymus which is of axonal, vesicular origin. In the present study neurochemical evidence was obtained that the release of NA is subject to ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00085-j
更新日期:1995-07-01 00:00:00
abstract::Tumor cells express a range of antigens including self-antigens (those whose expression is shared by normal host tissue) and non-self antigens (such as those that arise as a result of mutations in normal cellular genes or in the case of some tumors, viral antigens). Immune responses to both types of antigen have been ...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(02)00240-7
更新日期:2003-01-22 00:00:00
abstract::In contrast to defibrinated blood, cells isolated from heparinized blood give lower T-lymphoproliferative responses. Such responses can be also suppressed by addition of heparin to lymphocyte cultures. Platelets inhibit T cell proliferation and B cell differentiation, but their interactions with heparin may produce co...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90115-q
更新日期:1991-05-01 00:00:00
abstract::As one studies more and more the immune systems of invertebrates their multiplicity and diversity increase. On one hand, the number of different local and systemic innate mechanisms (or cascades of mechanisms) increases with every phylum studied, on the other hand the diversity increases within each system as multiple...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2003.10.012
更新日期:2004-03-29 00:00:00
abstract::Forkhead box O1 (FoxO1) has a crucial role in the early B cell development. To understand the functional importance of FoxO1 gene in the early B cell expansion, we established a FoxO1 knockdown model using 70Z/3 pre-B cell line. The FoxO1 knockdown 70Z/3 cells (70Z/3-KD cells) showed the down-regulated expression of i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2019.09.004
更新日期:2019-12-01 00:00:00
abstract::To investigate the in vivo role of complement component C5 it is common to compare the inflammatory response between C5-normal and C5-deficient inbred mice strains. Nevertheless, it should be expected that differences in the genetic backgrounds between those strains may affect several physiological parameters, complic...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.02.010
更新日期:2014-05-01 00:00:00
abstract::It is known that the receptors for the Fc portion of IgG molecules (Fc gamma R) are widely distributed in cells of the immune system. The expression of Fc gamma R enables monocytes and neutrophils to destroy antibody-coated target cells through the antibody-dependent cell-mediated cytotoxicity (ADCC) mechanism. In add...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90182-a
更新日期:1991-08-01 00:00:00
abstract::The antischistosomal property of a short course of cyclosporin A (CsA), administered at the time of infection was demonstrated in the rat. In comparison with vehicle-treated controls, no schistosomes were recovered from CsA-treated animals, 18-19 days post infection. Flow cytometric analysis of blood and spleen lympho...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90086-7
更新日期:1988-02-01 00:00:00
abstract::Two cell lines producing monoclonal antibodies have been established from peripheral blood of a negative Rhesus blood donor which has been immunized with positive Rhesus red blood cells. Two monoclonal antibodies Co II 8.8 and Co II 7.12 have been selected. Both are IgG1 antibodies, but recognize different epitopes on...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90046-5
更新日期:1986-09-01 00:00:00
abstract::In several diseases where the immune system plays an important role there has been a tremendous progress in treatment efficacy during the last decades. Based on necessary basic science these improvements are results of rapid, numerous and open-minded clinical trials where pieces of positive results step by step have b...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2014.02.006
更新日期:2014-05-01 00:00:00
abstract::Partially purified IgE receptor(s) of rat basophilic leukemia cells (RBL) designated R and H and having apparent molecular weight of 45,000 and 55,000 daltons, respectively, were subjected to proteolysis with papain. Polyacrylamide gel electrophoresis of the digests in the presence of sodium dodecyl sulfate revealed a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90029-3
更新日期:1982-03-01 00:00:00
abstract::We have investigated the cellular responses of guinea-pig macrophages to human C4a. C4a induced a biphasic Ca2+ mobilization; a rapid temporary Ca2+ mobilization from intracellular Ca2+ pool followed by a weak Ca2+ influx of extracellular Ca2+. Although the C4a-treated macrophages did not respond again to C4a, that is...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90104-a
更新日期:1993-06-01 00:00:00
abstract::Leukaemic blood lymphocytes from patients with B prolymphocytic leukaemia express the common acute lymphoblastic leukaemia antigen (CALLA). The in vitro incubation of prolymphocytes with leucocyte conditioned medium produced the acquisition of plasma cell morphology, of intracytoplasmic immunoglobulins and the simulta...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90005-7
更新日期:1985-01-01 00:00:00
abstract::Donor-reactive memory T cells mediated accelerated rejection is known as a barrier to the survival of transplanted organs. We investigated the combination of different monoclonal antibodies (mAbs) and donor-specific transfusion (DST) in memory T cells-based adoptive mice model. In the presence of donor-reactive memory...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2011.03.009
更新日期:2011-08-30 00:00:00
abstract::Previous studies have demonstrated that culture supernatants of LPS-stimulated murine splenic B lymphocytes (BLPSSN) are able to inhibit the growth of freshly isolated B cells via an IgM antibody. In this work we investigated the progress of LPS-activated B lymphocytes through the cell cycle in the presence of this an...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90070-5
更新日期:1992-08-01 00:00:00
abstract::The aim of this work was to raise allotype specific monoclonal antibodies to human CD45, with the long-term objective of producing a reagent which could be used to prolong graft survival in renal transplantation through removal of passenger leukocytes from the graft. At present there are no anti-CD45 monoclonal antibo...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00056-5
更新日期:1999-06-01 00:00:00
abstract::Monoclonal antibodies of the Orthoclone series were used to identify total T lymphocytes (OKT3) and their helper-inducer (OKT4) and cytotoxic-suppressor (OKT8) subsets in 25 patients with chronic Chagas' disease and 25 healthy controls. No significant difference in the number of total T cells (OKT3+) circulating in th...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90011-7
更新日期:1984-01-01 00:00:00
abstract::Antibody direct cloning from single B cells is simple and efficient and has been successful in antibody identification of infectious diseases. However, although a recent whole-exome sequencing revealed abundant heterogeneic mutation accumulation in cancers, identification and synthesis of autoantibodies against specif...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.02.004
更新日期:2014-05-01 00:00:00
abstract::Tuberculosis is an infectious disease that affects millions of people worldwide with an annual mortality rate of 1.3 million. The mechanisms contributing to the loss of balance of immune responses and progression to active tuberculosis disease are unknown. Although CD4+ and CD8+ T cells and the cytokines they produce ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.03.009
更新日期:2014-07-01 00:00:00