Abstract：
:We have previously reported the production of a series of human monoclonal antibodies reacting with mouse mammary tumor viral antigens as well as the human counterpart HuMTV antigen. Against two of these antibodies (B11 and 4.6/6), anti-idiotypic antibodies were generated, which appeared to be the internal image of viral antigen. Vaccination with the anti-idiotypic antibodies induced in mice humoral and cellular immunity against both MMTV and HuMTV. The current study describes a novel method to produce human anti-MMTV antibodies derived monoclonal antibodies. Normal peripheral blood lymphocytes (PBLs) were immunized in vitro in the presence of IL-2, with rabbit anti-B11 antibodies bound to silica beads. Following in vitro immunization, the lymphocytes were fused with the UC-792-6 human lymphoblastoid cell line. Four human hybridomas were found to secrete human monoclonal antibodies which bound to MMTV and HuMTV. Three of the secreted antibodies were of the IgG class and one of the IgM class. The specificity of binding was confirmed by direct and competitive ELISA assays and by radioimmunoprecipitation. Our study demonstrates the ability to generate human monoclonal anti-viral antibodies by in vitro immunization, employing "internal image" anti-idiotypic antibodies. The method can be used to generate human antibodies, especially against pathogenic agents or ill-defined antigens.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Blank M,Smorodinsky IN,Keydar I,Chaitchik S,Shoenfeld Ydoi
10.1016/0165-2478(91)90128-wsubject
Has Abstractpub_date
1991-04-01 00:00:00pages
65-71issue
1eissn
0165-2478issn
1879-0542pii
0165-2478(91)90128-Wjournal_volume
28pub_type
杂志文章abstract：:Alpha-fetoprotein (AFP), an onco-fetal protein which binds specifically polyunsaturated fatty acids, has been shown to modulate arachidonic acid metabolism in a human T-cell line. In Jurkat lymphoma cells, AFP decreases the synthesis of prostaglandins and increases the release of arachidonic acid and leukotrienes. The...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(87)90092-7
更新日期：1987-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(98)00014-5
更新日期：1998-04-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(86)90122-7
更新日期：1986-08-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2003.11.025
更新日期：2004-03-29 00:00:00
abstract：:It is well-established that chemokines play a critical role in the orchestration of inflammation and immunity. Interactions between chemokines and their receptors are essential for the homing of specific subsets of leukocytes to their functional microenvironments. They also influence other diverse biological processes...
journal_title：Immunology letters
pub_type： 杂志文章,评审
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更新日期：2005-01-31 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.06.007
更新日期：2009-08-15 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.imlet.2019.02.004
更新日期：2019-04-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00209-2
更新日期：2003-01-02 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2008.03.001
更新日期：2008-06-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
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更新日期：1996-06-01 00:00:00
abstract：:Mice lacking suppressor of cytokine signaling 1 (SOCS1) accumulate CD8(+) T lymphocytes in the thymus and in the periphery. Whereas IL-7 and IL-15 promote the generation of CD8 single positive (SP) thymocytes, IL-15 drives the expansion of CD8 T cells in the periphery. Here, we investigated whether increased productio...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2010.04.009
更新日期：2010-08-16 00:00:00
abstract：:The aim of the present letter is to propose a rational nomenclature for the d-mannose-binding lectin from seeds of Artocarpus integrifolia. It is justified by the existing confusion in the literature concerning the trivial names used until now to designate the lectin and by the increasing interest in its biomedical ap...
journal_title：Immunology letters
pub_type： 信件
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更新日期：2008-08-15 00:00:00
abstract：:RasGRP1 links TCR signaling to Ras in T cells, while both RasGRP1 and RasGRP3 link BCR signaling to Ras in B cells. T cells deficient in RasGRP1 have defective proliferative responses as do B cells deficient in both RasGRP1 and RasGRP3, confirming the importance of Ras activation in lymphocyte proliferation. While age...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.01.005
更新日期：2006-05-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.02.005
更新日期：2013-03-01 00:00:00
abstract：:Human deficiency in transporter associated with antigen processing (TAP) is characterized by a very low surface expression of human leukocyte antigen (HLA) class I molecules in hematopoietic and non hematopoietic cells. Among the latter, TAP-deficient skin fibroblasts have previously been shown by us to be very sensit...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.07.010
更新日期：2006-11-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2013.07.004
更新日期：2013-06-01 00:00:00
abstract：BACKGROUND AND PURPOSE:Polygonum orientale L. (family: Polygonaceae), named Hongcao in China, has effects of dispelling wind and dampness, promoting blood circulation, and relieving pain. Our group has already studied and confirmed that POEa and POEe (ethyl acetate and ethyl ether extract of P. orientale, respectively)...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2018.11.009
更新日期：2018-09-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(01)00341-8
更新日期：2002-04-22 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90016-1
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journal_title：Immunology letters
pub_type： 杂志文章,评审
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更新日期：2014-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2000-05-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(88)90086-7
更新日期：1988-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2003.09.004
更新日期：2003-12-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(89)90124-7
更新日期：1989-12-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90048-f
更新日期：1991-11-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(87)90071-x
更新日期：1987-05-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.08.007
更新日期：2017-10-01 00:00:00
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journal_title：Immunology letters
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.imlet.2011.06.008
更新日期：2011-12-30 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2010.06.002
更新日期：2010-08-16 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2003.10.004
更新日期：2004-01-30 00:00:00
