Abstract:
:Antibody direct cloning from single B cells is simple and efficient and has been successful in antibody identification of infectious diseases. However, although a recent whole-exome sequencing revealed abundant heterogeneic mutation accumulation in cancers, identification and synthesis of autoantibodies against specific cancer-associated antigens is still difficult in cancer patients owing to the very small number of B cells producing autoantibodies. In the present study, to identify autoantibodies targeting tumor antigens, we measured the titer of autoantibodies in high-grade glioma patients' plasma and identified two patients with elevated autoantibodies to a few transmembrane proteins. Specific B cells producing autoantibody against vascular endothelial growth factor receptor (VEGFR) 2 were immunostained with labeled protein and anti-human IgG antibody, and then collected by a single cell sorter. Finally, 22 antibody genes were successfully identified using direct IgG cloning from single B cell mRNA, and two antibody clones were found to have significant VEGFR2-specific binding affinity. The current direct human IgG gene cloning technique for identifying human antibodies derived from IgG-memory B cells avoids time-consuming procedures such as phage display-based antibody-library screening, and therefore may be applicable to identifying human autoantibodies in a variety of disorders including cancers even when antibody elevation is not detected because of a very small number of memory B cells.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Iizuka A,Komiyama M,Oshita C,Kume A,Ashizawa T,Mitsuya K,Hayashi N,Nakasu Y,Yamaguchi K,Akiyama Ydoi
10.1016/j.imlet.2014.02.004subject
Has Abstractpub_date
2014-05-01 00:00:00pages
15-22issue
1-2eissn
0165-2478issn
1879-0542pii
S0165-2478(14)00026-1journal_volume
159pub_type
杂志文章abstract::The anaerobic bacterium Campylobacter pylori (Cp) is thought to be associated with chronic gastritis. This paper presents clinical data underpinning this view. Five patients with histological chronic gastritis as determined by diagnostic endoscopy, which was associated with Cp as determined by positive biopsy cultures...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90069-2
更新日期:1989-01-15 00:00:00
abstract::Chagas' disease is accompanied by severe anemia and oxidative stress, which may contribute to mortality. In this study, we investigated the role of 5-lipoxygenase (5-LO) in the control of parasitism and anemia associated with oxidative damage of erythrocytes in experimental Trypanosoma cruzi infection. Wild-type C57BL...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.02.002
更新日期:2009-03-24 00:00:00
abstract::Pretreatment with interleukin (IL)-10 inhibited the release of growth-related oncogene GRO-alpha but not of epithelial-cell derived neutrophil activating protein (ENA)-78, after injection of lipopolysaccharide (LPS) into healthy humans. In vitro, IL-10 dose-dependently attenuated LPS-induced release of both GRO-alpha ...
journal_title:Immunology letters
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0165-2478(01)00224-3
更新日期:2001-08-01 00:00:00
abstract::Homogeneous IgG fractions were obtained by chromatography of the sera of patients with multiple sclerosis (MS) on Protein G-Sepharose under conditions that remove non-specifically bound proteins. These IgGs contained several chelated metals, the relative amount of which decreases in the order: Fe>or=Ca>Cu>or=Zn>or=Mg>...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.10.018
更新日期:2006-02-28 00:00:00
abstract::Abnormal activation of the transcriptional factor STAT3 (signal transducer and activator of transcription 3) was recently associated with Alzheimer Disease (AD). STAT3 phosphorylation is critical for cytokine secretion linked to neuroinflammation. Moreover, STAT3 may act as a transcriptional regulator of BACE1 (β-APP ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.10.004
更新日期:2020-12-01 00:00:00
abstract::The T cell receptor of gamma delta is normally expressed on a small percentage of peripheral lymphocytes. Although the role of gamma delta T cells in the physiologic immune response is still unknown, there is accumulating evidence that gamma delta T cells may participate in the immune response to mycobacterial and oth...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90105-y
更新日期:1990-08-01 00:00:00
abstract::Autoimmune hepatitis (AIH) is a chronic liver disease mediated by immunity, and could lead to liver fibrosis and hepatocellular carcinoma. However, the mechanisms for breaking hepatic tolerance and driving AIH still remain elusive. We herein reported that the non-specific liver inflammation triggered by carbon tetrach...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2019.11.010
更新日期:2020-02-01 00:00:00
abstract::We infected umbilical cord blood lymphocytes (CBL) with EBV in vitro. Analysis of the cell population in 3- and 6-day-old cultures showed a relative increase of B cells and outgrowth of B cells after prolonged culture period. The immunomodulator PSK was added to parallel cultures. In these cultures, B cell growth was ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00330-3
更新日期:2002-03-01 00:00:00
abstract::In transplant rejection, graft versus host or autoimmune diseases T cells are mediating the pathophysiological processes. Compared to unspecific pharmacological immune suppression specific inhibition of those T cells, that are involved in the disease, would be an alternative and attractive approach. T cells are activa...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.06.002
更新日期:2009-08-15 00:00:00
abstract::Phagocytosis of latex beads by peritoneal macrophages was examined by means of flow cytometry (FCM). This assay revealed that adrenocorticotropic hormone (ACTH) suppressed phagocytosis in a dose-dependent manner. ACTH (1-24) was more suppressive than ACTH (1-39). Control phagocytosis was partially suppressed in Ca(2+)...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90080-9
更新日期:1994-10-01 00:00:00
abstract::Many types of cells in the immune system have been found to produce nitric oxide (NO), which performs multiplex functions. However, in myelin basic protein peptide 68-86 (MBP 68-86)-induced experimental autoimmune encephalomyelitis (EAE) in Lewis rats, we found that elevated NO production was generated from spleen cel...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00154-1
更新日期:2000-03-01 00:00:00
abstract::Members of the transforming growth factor beta (TGF-beta) superfamily are soluble factors that regulate a variety of functional responses including proliferation, differentiation, apoptosis and cell cycle, among others, depending not only on the cell type and its differentiation state, but also on the milieu of cytoki...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2006.12.010
更新日期:2007-03-15 00:00:00
abstract::Transforming growth factor-beta (TGF-beta) is usually known as an immunosuppressive cytokine, but we and others have shown stimulatory effects of TGF-beta on activation of Th2 T-lymphocytes. In the present investigation we have studied the effect of TGF-beta1 on phosphorylation of ERK, a MAP-kinase downstream of the R...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00184-x
更新日期:2000-06-01 00:00:00
abstract::Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multifaceted hematopoietic cytokine and the culture of mouse bone marrow with GM-CSF produces a variety of myeloid cells including granulocytes, macrophages, and dendritic cells. In the present study, we cultured mouse splenocytes with GM-CSF and examined ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.03.003
更新日期:2016-05-01 00:00:00
abstract::Administration of human recombinant interleukin-2 (RIL-2) into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of HeLa, HU 609T and T24B human carcinoma cells partially inhibited growth of the human tumor xenografts. In vitro activation of nu/nu spleen cells with human RIL-2 resulted in generation ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90013-7
更新日期:1987-04-01 00:00:00
abstract::The monocytic cell line I937 was derived from U937 by sorting for cells with high expression of MHC class II molecules. Further analysis of these class II molecules revealed the presence of the HLA-DR3 subtype suggesting that the cell line was a potential candidate for testing antigen presentation to T cells restricte...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)02478-6
更新日期:1996-01-01 00:00:00
abstract::A TNF-like factor was purified from lipopolysaccharide (LPS) induced New Zealand white rabbit serum. The TNF-like factor was purified by DEAE-Sephacel, Sephacryl S-200, Mono-Q, CM-affi gel Blue, Superose 12 H/R preparative columns to the specific activity of 4 x 10(6) U/mg protein. The purified protein was 45 kDa in i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00134-x
更新日期:1998-02-01 00:00:00
abstract::The physiological role(s) of fetuin, a protein present in plasma and many tissues of developing animals at levels much higher than in the adult, is unknown. Here we show that fetuin can modify the responses of macrophages to lipopolysaccharide (LPS) stimulation. At concentrations of fetuin in the medium, corresponding...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00126-0
更新日期:1998-01-01 00:00:00
abstract:PURPOSE:Benazepril, an angiotensin-converting enzyme (ACE) inhibitor, has been used to treat hypertension, congestive heart failure, and chronic renal failure. However, its biological activity and mechanism of action in inflammation are not fully identified. The present study was designed to determine the in vivo anti-...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.05.005
更新日期:2013-05-01 00:00:00
abstract::It is well-established that chemokines play a critical role in the orchestration of inflammation and immunity. Interactions between chemokines and their receptors are essential for the homing of specific subsets of leukocytes to their functional microenvironments. They also influence other diverse biological processes...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2004.08.018
更新日期:2005-01-31 00:00:00
abstract::Interleukin (IL)-17A is a cytokine that plays an important role in infectious, autoimmune, and inflammatory diseases. In this study, we found that TCRγδ(+)CD4(-)CD8(-) T cells, but not TCRαβ(+)CD4(+) T cells, are the primary producers of IL-17A in the genital tract of female mice in the steady-state condition. High mR...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2012.05.006
更新日期:2012-09-01 00:00:00
abstract::The HIV-1 envelope protein contains several regions with amino acid homology to HLA class I and class II molecules. We evaluated possible changes in antibody responses to those regions during vaccination with rgp 160 produced in a baculovirus system. Forty asymptomatic HIV-infected patients with CD4 cell counts above ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90125-2
更新日期:1994-07-01 00:00:00
abstract::The tryptophan fluorescence properties of two different peptide complexes of the single-chain H-2Kd (SC-Kd) were studied by means of the single-photon counting technique. The latter enables time-resolved measurements of fluorescence intensity and anisotropy decay parameters relevant to structural and dynamic propertie...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90183-x
更新日期:1994-05-01 00:00:00
abstract::Establishment and maintenance of pregnancy represents a challenge for the maternal immune system since it has to defend against pathogens and tolerate paternal alloantigens expressed in fetal tissues. Regulatory T (Treg) cells, a subset of suppressor CD4(+) T cells, play a dominant role in the maintenance of immunolog...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2014.06.013
更新日期:2014-11-01 00:00:00
abstract::An effective HIV vaccine will likely need to induce potent and broad-based immunity, including CD8+ T cell responses. Hence, a quantitative assay to measure such responses in animal models will be important in the evaluation of candidate HIV vaccines. We show here that a single immunization with HIV DNA vaccines, foll...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00237-7
更新日期:2003-01-22 00:00:00
abstract::Direct interaction between Fusobacterium nucleatum and human neutrophils resulted in the secretion of a neutrophil self-regulatory factor(s). The secretion of this factor was bacteria specific, and depended on the integrity of the bacteria cell surface. Factor secretion occurred within 15 min of bacteria--neutrophil i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90086-1
更新日期:1987-01-01 00:00:00
abstract::When human peripheral blood lymphocytes (PBL) are cultured with either concanavalin A (Con A)-treated or control autologous T lymphocytes, the mitogenic responses of the PBL co-cultured with Con A-treated cells are much lower. We have investigated the cell surface receptor changes during culture of T cells with and wi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90031-2
更新日期:1984-01-01 00:00:00
abstract::Pentoxifylline (PTX), a methylxanthine derivative and phosphodiesterase inhibitor, is known to influence production and/or function of some cytokines. We examined the effect of PTX on the in vitro expression of cytokine genes using endotoxin- or phytohaemagglutinin (PHA)-stimulated human blood mononuclear cells. The e...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90022-t
更新日期:1993-08-01 00:00:00
abstract::African swine fever (ASF) virus has been reported to infect cells of the monocyte family, probably macrophage-like cells, but there is variation in the apparent susceptibility of these cells. We have demonstrated that the phenotype and activity of porcine monocytic cells varies between different isolations and also up...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90136-p
更新日期:1993-07-01 00:00:00
abstract::A novel mutant strain of rats, LEC, exhibits an arrest of T-cell maturation from CD4+CD8+ to CD4+CD8- but not the CD4-CD8+ cells in the thymus. Nevertheless, CD4+T cells arise in lymph nodes of LEC rats, implying extrathymic T-cell maturation. We analyzed the variable (V) and junctional region diversity of T-cell rece...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)00254-o
更新日期:1995-03-01 00:00:00