Abstract：
:Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multifaceted hematopoietic cytokine and the culture of mouse bone marrow with GM-CSF produces a variety of myeloid cells including granulocytes, macrophages, and dendritic cells. In the present study, we cultured mouse splenocytes with GM-CSF and examined the changes in hematopoietic cell populations over a week. Most of the splenic hematopoietic cells disappeared significantly from culture within 6days with or without the presence of GM-CSF. Among the splenic granulocyte populations, only eosinophils fully survived throughout the culture with GM-CSF for more than a week. During 10days of culture with GM-CSF, splenic eosinophils maintained their morphology as well as most of their surface molecules at high levels, including CCR3 and Siglec F. Meanwhile, the expression of mRNAs encoding major basic protein-1 (MBP-1) and eosinophil peroxidase (EPO), two major eosinophil-derived granule proteins, was diminished significantly from the cultured eosinophils. EPO assays also revealed that eosinophils in culture for more than 5days retained 30% or less EPO activity compared to those in uncultured splenocytes. In contrast, culture of splenocytes with GM-CSF did not change the capacity of eosinophils to migrate in response to eotaxin-1. Our results indicate that mouse splenic eosinophils are effectively cultured for lengthy periods while their expression of eosinophil-derived granule proteins is specifically suppressed. The relevance of these findings to eosinophilic inflammatory response is discussed.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Ryu SH,Na HY,Sohn M,Han SM,Choi W,In H,Hong S,Jeon H,Seo JY,Ahn J,Park CGdoi
10.1016/j.imlet.2016.03.003subject
Has Abstractpub_date
2016-05-01 00:00:00pages
7-20eissn
0165-2478issn
1879-0542pii
S0165-2478(16)30028-1journal_volume
173pub_type
杂志文章abstract：:Mouse spleen cells were activated with IL2 for 4 days in the presence or absence of paraformaldehyde fixed YAC (PFY) tumor cells (spleen cell: PFY ratio 100:1). Fresh spleen cells had poor expression of Ly49A but the expression was significantly upregulated by IL2 activation. Addition of PFYs resulted in a further boo...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(01)00206-1
更新日期：2001-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90048-f
更新日期：1991-11-01 00:00:00
abstract：:Mimotopes derived from peptide phage display libraries may reproduce basic functions of epitopes including their antigenicity. In case of toxins, this property makes phage displayed mimotopes highly specific vaccine components free of the toxicity. To explore the potential of mimotopes for vaccine development, their a...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(01)00320-0
更新日期：2002-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90051-5
更新日期：1994-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2007.02.005
更新日期：2007-04-15 00:00:00
abstract：:Interleukin 1 beta (IL1 beta) is an inducible polypeptide with many roles in host defence and homoeostasis. It has also been implicated as a mediator of infectious, inflammatory and autoimmune diseases, and the kinetics of its production are relevant to an understanding of the pathogenesis of these conditions. We repo...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90172-7
更新日期：1991-08-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2004.04.015
更新日期：2004-06-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90178-6
更新日期：1985-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90115-4
更新日期：1985-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
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更新日期：2020-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(87)90171-4
更新日期：1987-12-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2017.11.014
更新日期：2018-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.08.007
更新日期：2013-07-01 00:00:00
abstract：:Phosphatidylinositol 3-kinase (PI3K) is critical player in cell proliferation and survival. The effects of LY294002 and wortmannin, inhibitors of PI3K, on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipoploysaccharide (LPS)-induced Raw 264.7 cells were investigated. Significan...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.12.007
更新日期：2005-06-15 00:00:00
abstract：:Scid mice develop human EBV-positive B cell tumors after injection with EBV-transformed human B cells or peripheral blood lymphocytes from EBV-seropositive donors. Injection of cytotoxic T cell (CTL) lines, selectively expanded in vitro to recognize autologous EBV-transformed B cells, protected mice against developing...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02602-8
更新日期：1996-09-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90099-v
更新日期：1991-09-01 00:00:00
abstract：:Natural Killer (NK) cells were discovered when research on T cells produced an unexplained background, or "natural" cytotoxicity in non-immunized mice. The previously unknown cells were thus named "natural" killer cells, or NK cells. They are roughly defined by a lack of the T cell marker CD3 and a presence of CD56 (o...
journal_title：Immunology letters
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.imlet.2017.07.014
更新日期：2017-10-01 00:00:00
abstract：:The human homologue of 4-1BB (H4-1BB) cDNA was isolated from PMA plus ionomycin-treated human peripheral T-cell cDNA libraries. The amino acid sequence deduced from the nucleotide sequence showed that the protein is composed of 255 amino acids with 2 potential N-linked glycosylation sites. The molecular weight of its ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)00227-i
更新日期：1995-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：2002-04-22 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2010.06.002
更新日期：2010-08-16 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.07.014
更新日期：2016-09-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(98)00077-7
更新日期：1998-11-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(88)90042-9
更新日期：1988-03-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90035-o
更新日期：1990-03-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90151-8
更新日期：1985-01-01 00:00:00
abstract：:This paper describes functional and genetic studies on the macrophage resistance gene Lsh/Ity/Bcg first described almost two decades ago. Working in vitro with resident peritoneal, liver (Kupffer cells) and bone marrow derived macrophages from congenic B10 (LshS) and B10.L-LshR mice it has been possible to demonstrate...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(91)90032-6
更新日期：1991-10-01 00:00:00
abstract：:Using the method of estimation of alpha-helical and beta-folded types of secondary structure from amino acid composition, it has been established that interferons contain about 60-70% of alpha-helices and not more than 10-20% of beta-structure. The same result was obtained using empirical and stereochemical rules for ...
journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1982-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(00)00206-6
更新日期：2000-10-03 00:00:00
abstract：:CD27, which belongs to the TNF receptor family, is a costimulatory molecule that participates in T-cell activation. Unlike costimulatory molecules such as OX40 and 4-1BB, little is known about the role CD27 plays a role in the development of experimental diseases. We asked whether CD27 and its ligand CD70 participate ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2008.05.004
更新日期：2008-08-15 00:00:00
abstract：:Peripheral blood from 11 newly diagnosed patients with insulin-dependent diabetes mellitus (IDDM) was studied for the proportion of monoclonal antibody (HNK 1, Leu 7) defined natural killer (NK) cells using a fluorescence-activated cell sorter analyzer. The proportion of Leu 7+ cells in patients with IDDM (7.0 +/- 4.0...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90056-7
更新日期：1984-01-01 00:00:00
