Abstract：
:The human homologue of 4-1BB (H4-1BB) cDNA was isolated from PMA plus ionomycin-treated human peripheral T-cell cDNA libraries. The amino acid sequence deduced from the nucleotide sequence showed that the protein is composed of 255 amino acids with 2 potential N-linked glycosylation sites. The molecular weight of its protein backbone is calculated to be 27 kDa. The H4-1BB contains features such as signal sequence and transmembrane domain, indicating that it is a receptor protein. This protein showed 60% identity of amino acid sequence to mouse 4-1BB. In the cytoplasmic domain there are 5 regions of amino acid sequences conserved from mouse to human, indicating that these residues might be important in the 4-1BB function. H4-1BB mRNA was detected in unstimulated peripheral blood T cells and was inducible in T-cell lines such as Jurkat and CEM. H4-1BB-AP, a fusion protein between the H4-1BB extracellular domain and alkaline phosphatase, was used to identify the ligand for the H4-1BB. Although the H4-1BB ligand was detected in both T and B cells of human peripheral blood, the ligand was preferentially expressed in primary B cells and B-cell lines. Daudi, a B-cell lymphoma, was one of the B-cell lines that carried a higher number of ligands. Scatchard analysis showed that the Kd = 1.4 x 10(9) M and the number of ligands in Daudi cell was 4.2 x 10(3).
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Zhou Z,Kim S,Hurtado J,Lee ZH,Kim KK,Pollok KE,Kwon BSdoi
10.1016/0165-2478(94)00227-isubject
Has Abstractpub_date
1995-02-01 00:00:00pages
67-73issue
1-2eissn
0165-2478issn
1879-0542pii
0165-2478(94)00227-Ijournal_volume
45pub_type
杂志文章abstract：:Differentiation between a non-infective and an infective Leishmania promastigote population was demonstrated. Promastigotes in the stationary phase (day 5) were found to be highly infective in vitro to BALB/c mouse peritoneal macrophages, compared with those of the logarithmic phase (day 3). The infective promastigote...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90114-2
更新日期：1985-01-01 00:00:00
abstract：:We aimed to investigate if angiopoietin-2 (Ang-2) participates in the septic process and what may be the role of monocytes as a site of release of Ang-2 in sepsis. Concentrations of Ang-2 were estimated in sera and in supernatants of monocytes derived form one already described cohort of 90 patients with septic syndro...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.06.006
更新日期：2009-06-30 00:00:00
abstract：:In contrast to defibrinated blood, cells isolated from heparinized blood give lower T-lymphoproliferative responses. Such responses can be also suppressed by addition of heparin to lymphocyte cultures. Platelets inhibit T cell proliferation and B cell differentiation, but their interactions with heparin may produce co...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90115-q
更新日期：1991-05-01 00:00:00
abstract：:At least three B cell subsets, B-1a (Ly-1B), B-1b and B-2, are present in the mouse periphery. B-1a and B1-b cells represent a small population in the adult spleen and are abundant in the peritonial and pleural cavities. It has been demonstrated in our laboratory that B-1b cells spontaneously proliferated in stationar...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00266-3
更新日期：2003-03-03 00:00:00
abstract：:Cultured T lymphocytes from pig blood can be activated by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Activation is additive with that induced by the mitogenic lectin phytohaemagglutinin (PHA). Activation by TPA differs from that induced by PHA or other mitogenic lectins in that it is not inhibited e...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(83)90063-9
更新日期：1983-01-01 00:00:00
abstract：:Malaria, a major endemic tropical disease, is caused by the infection of blood cells by Plasmodium protozoa. Most patients control their parasitemia by a not fully understood spleen-dependent mechanism. SDF-1alpha is a chemokine produced by stromal cells such as reticular spleen cells. Nitric oxide (NO) has several im...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2003.05.001
更新日期：2003-10-31 00:00:00
abstract：:The majority of peripheral blood mononuclear cells (PBMC) from acute-phase infectious mononucleosis (IM) patients express high levels of T10 (CD38) and HLA-DR surface antigens, which are markers characteristic of activated T cells. However, Tac antigen (p55) expression on these cells was not detectable by flow cytomet...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(89)90126-0
更新日期：1989-12-01 00:00:00
abstract：:Interleukin 2 (IL 2) is a lymphocyte growth factor capable of inducing the rapid growth of antigen- or mitogen-stimulated T cells. To understand the molecular mechanisms of IL 2 action, we studied the synthesis of cellular polypeptides induced by IL 2. For a homogeneous cell population responsive to IL 2, we used a mu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90115-4
更新日期：1985-01-01 00:00:00
abstract：:The aim of the present letter is to propose a rational nomenclature for the d-mannose-binding lectin from seeds of Artocarpus integrifolia. It is justified by the existing confusion in the literature concerning the trivial names used until now to designate the lectin and by the increasing interest in its biomedical ap...
journal_title：Immunology letters
pub_type： 信件
doi：10.1016/j.imlet.2008.06.002
更新日期：2008-08-15 00:00:00
abstract：:Both, HIV envelope proteins gp120 and gp41 can directly activate complement system, even in the absence of HIV-specific antibodies. During the budding process HIV acquires host membrane-associated molecules among these complement regulatory proteins (CRPs). The presence of CRPs on the viral surface rescues HIV from co...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2004.11.007
更新日期：2005-03-15 00:00:00
abstract：:Melanoma is a prototype of immunogenic tumor to which various types of immunotherapy have been applied extensively over the past decades. Melanoma vaccines are designed for the purpose of immune modulation and subsequent anti-tumor effects in the process of an active specific immunotherapy. Previous attempts of these ...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/s0165-2478(00)00251-0
更新日期：2000-09-15 00:00:00
abstract：:Complement factor I (CFI) deficiency is typically associated to recurrent infections with encapsulated microorganisms and, less commonly, to autoimmunity. We report a 53-years old male who, in a routine control for non-alcoholic fatty liver disease, presented a flat beta-2 fraction at the capillary protein electropher...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.04.011
更新日期：2016-06-01 00:00:00
abstract：:Two cell lines producing monoclonal antibodies have been established from peripheral blood of a negative Rhesus blood donor which has been immunized with positive Rhesus red blood cells. Two monoclonal antibodies Co II 8.8 and Co II 7.12 have been selected. Both are IgG1 antibodies, but recognize different epitopes on...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(86)90046-5
更新日期：1986-09-01 00:00:00
abstract：:Allogeneic, H-2-incompatible irradiation chimeras (H-2d leads to H-2b) constructed with normal, unmanipulated bone marrow and with marrow-derived factors live long and do not manifest a GvH disease. Their response to primary immunization is deficient but their alloreactivity is normal. This chimeric allotolerance cann...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(83)90003-2
更新日期：1983-04-01 00:00:00
abstract：:It is widely known that mast cells and cytokine TNF-alpha are both involved in inflammatory reactions. Therefore, we have studied whether TNF-alpha can cause histamine secretion from human adenoidal and cutaneous mast cells. The experiments were performed in vitro on mast cells isolated from tissues by enzymatic dispe...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00115-6
更新日期：1997-12-01 00:00:00
abstract：:An effective HIV vaccine will likely need to induce potent and broad-based immunity, including CD8+ T cell responses. Hence, a quantitative assay to measure such responses in animal models will be important in the evaluation of candidate HIV vaccines. We show here that a single immunization with HIV DNA vaccines, foll...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00237-7
更新日期：2003-01-22 00:00:00
abstract：:Crohn's disease and ulcerative colitis, known together as inflammatory bowel disease (IBD), are severe autoimmune disorders now causing gut inflammation and ulceration, among other symptoms, in up to 1 in 250 people worldwide. Incidence and prevalence of IBD have been increasing dramatically over the past several deca...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2014.07.014
更新日期：2014-12-01 00:00:00
abstract：:RASI-1 is a novel matrix metalloproteinase which we isolated from an expression cDNA library representing the mRNA of an inflamed synovium obtained from a patient with rheumatoid arthritis (RA). To investigate the involvement of RASI-1 in the pathology of RA, we examined synovial specimens from RA patients with antibo...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00057-6
更新日期：1997-06-01 00:00:00
abstract：:Human milk lactotransferrin at a concentration ranging from 1 to 10 micrograms/ml stimulated up to 5 times the humoral immune response to sheep red blood cells, expressed as the number of plaque-forming cells, when injected into mice 3 h before immunization. Further, lactotransferrin-treated thymocytes given intraveno...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90099-v
更新日期：1991-09-01 00:00:00
abstract：:Indoleamine 2,3-dioxygenase (IDO) is an enzyme with an immunosuppressive effect whose function is diverted by tumor cells to counteract immune cell functions, inducing immune escape of tumor cells. The aim of this study was to investigate the clinical significance of IDO in nasopharyngeal carcinoma (NPC). Compared to ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2015.11.012
更新日期：2016-01-01 00:00:00
abstract：:Thymocyte conditioned medium (TCM) was prepared using 2 lots of fetal calf, and 1 lot each of horse and rabbit sera. The 4 TCM and the respective sera alone were compared in their ability to support primary in vitro sensitization to sheep red blood cells (SRBC) as assayed by direct plaque formation. As would be expect...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(87)90084-8
更新日期：1987-01-01 00:00:00
abstract：:Studies on natural cytotoxicity of peripheral blood monocytes were conducted in patients with acute viral hepatitis B, patients with chronic aggressive hepatitis etiopathogenically linked to viral hepatitis B, and in asymptomatic carriers of HBs antigen. In the majority of cases of viral hepatitis in the acute stage o...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90016-1
更新日期：1985-01-01 00:00:00
abstract：:Natural levels of goat antiphosphorylethanolamine (anti-PE) antibodies decreased after injection of purified human C-reactive protein (CRP). The concentration of anti-CRP antibodies followed a continuous increment from non-detectable levels to a plateau at 80 days after first injection. ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(88)90070-3
更新日期：1988-05-01 00:00:00
abstract：:Mouse trisomy 16 (Ts16), an animal model for human Down syndrome (trisomy 21), exhibits severe abnormalities in the development of lymphoid and myeloid cells. Whereas fetal liver cells from diploid mice can be easily immortalized by retroviral transformation with Ab1-MuLV or J2 virus, fetal livers from Ts16 mice conta...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(92)90092-3
更新日期：1992-06-01 00:00:00
abstract：:The purpose of this study is to show that anti-Leu M1 antibody (anti-CD15), which has different staining characteristics in lymphoid and non-lymphoid cells, reacted against the surface antigen of a defined monoclonal B cell line. This antibody recognizes the sugar moiety, lacto-N-fucopentaose (LNF-III), which is linke...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(89)90153-3
更新日期：1989-11-01 00:00:00
abstract：:ErbB2, a member of the EGF receptor family of tyrosine kinases is overexpressed on many tumor cells of epithelial origin and is the molecular target of trastuzumab (Herceptin), the first humanized antibody used in the therapy of solid tumors. Trastuzumab, which is thought to act, at least in part, by downregulating Er...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2005.11.018
更新日期：2006-04-15 00:00:00
abstract：:Toll-like receptors (TLR) are critical in the activation of macrophages by bacterial products. It has been shown that TLR2 and TLR4 mediate lipopolysaccharide (LPS) and lipoproteins signal transduction, respectively. Regulation of TLR2 and TLR4 expression by LPS was considered to be one of the mechanisms to control th...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00010-x
更新日期：2002-05-01 00:00:00
abstract：:Interleukin-37 (IL-37) has been identified as a novel anti-inflammatory cytokine. The present study aimed to evaluate the expression of IL-37 in serum and in peritoneal fluid to determine its clinical significance in endometriosis. Enzyme-linked immunosorbent assay (ELISA) was performed to examine serum IL-37 levels i...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.03.012
更新日期：2017-05-01 00:00:00
abstract：:Part of pathogen-associated molecular pattern (PAMP) and damage-associated molecular pattern (DAMP) activate antigen-presenting cells through Toll-like receptors (TLRs) to initiate immune responses. However, controversy remains if TLR4 mediates DAMP signaling due to the confounding effects of potential LPS contaminati...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.05.006
更新日期：2009-06-30 00:00:00
abstract：:Serum obtained from mice infected with Trypanosoma cruzi have antibodies able to induce immune lysis in presence of complement. Gel filtration in Sephadex G-200 and affinity chromatography with rabbit anti-mouse IgG and protein A-Sepharose showed that the antibodies responsible for the immune serum lytic activity are ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(83)90040-8
更新日期：1983-01-01 00:00:00
