Abstract:
:Interleukin 2 (IL 2) is a lymphocyte growth factor capable of inducing the rapid growth of antigen- or mitogen-stimulated T cells. To understand the molecular mechanisms of IL 2 action, we studied the synthesis of cellular polypeptides induced by IL 2. For a homogeneous cell population responsive to IL 2, we used a murine clone of natural killer-like cells, NK 7, which is strictly dependent on exogenous IL 2 for cell viability and growth. NK 7 cells were first kept in culture without IL 2 for 6 h and then labeled for 3 h with [14C]leucine in the presence or absence of 100 U/ml recombinant human IL 2. Analysis of the cellular polypeptides by two dimensional gel electrophoresis and autoradiography revealed that the addition of IL 2 to IL 2-starved NK 7 cells specifically increased the levels of at least ten polypeptides (designated from molecular weight as p94a, p94b, p52, p51a, p51b, p48.5, p36, p34.5, p25.5, and p23). The increases of p52, p25.5, and p23 were inhibited by 5 micrograms/ml actinomycin D, whereas the other seven were virtually unaffected by the drug.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Yoshie O,Wada J,Ishida Ndoi
10.1016/0165-2478(85)90115-4subject
Has Abstractpub_date
1985-01-01 00:00:00pages
325-30issue
5-6eissn
0165-2478issn
1879-0542pii
0165-2478(85)90115-4journal_volume
11pub_type
杂志文章abstract::Fibrous plaques and intimal thickenings of 5 femoral and 5 iliac human arteries obtained at surgery were processed for indirect and double-labeling immunoelectron microscopy using an affinity purified rabbit IgG anti-C5b-9 neoantigen and the EBM 11 monoclonal antibody anti-human macrophages. The C5b-9 complexes were l...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90039-4
更新日期:1989-03-01 00:00:00
abstract::Recombinant gp120 of the HIV-1(IIIB) isolate (BH10 clone) has been mutated to form the PR12 protein with the first 74 C-terminal amino acids and the V1, V2 and V3 hypervariable loops deleted. A variety of studies have shown that the CD4 binding domain (CD4bd) is very well exposed in PR12 in contrast to rgp120(LAI). Us...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00289-9
更新日期:2001-12-03 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90086-7
更新日期:1988-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90021-0
更新日期:1986-11-17 00:00:00
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journal_title:Immunology letters
pub_type: 信件
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更新日期:2009-09-22 00:00:00
abstract::We have mapped a T cell epitope in the circumsporozoite (CS) protein of the murine malaria parasite, Plasmodium yoelii. A 21-mer synthetic peptide corresponding to the amino acid positions 59-79 (referred to as Py1), induced specific proliferation in BALB/c and C57BL/6 mice, and provided help for the production of ant...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90092-5
更新日期:1990-08-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2019.02.005
更新日期:2019-11-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.08.005
更新日期:2013-07-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90071-x
更新日期:1987-05-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.07.014
更新日期:2016-09-01 00:00:00
abstract::A series of nor-MDP analogues were evaluated for adjuvanticity in rodents using beta hCG-TT conjugate as the antigen. Of these, one compound, N-acetylnor-muramyl-L-N-methylalanyl-D-isoglutamine octylamide (nor-MDP octylamide (N-Me-Ala] was found to be effective. This compound, formulated with beta hCG-TT in water-in-o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90244-5
更新日期:1991-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2012.01.001
更新日期:2012-02-29 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90128-w
更新日期:1991-04-01 00:00:00
abstract::Interleukin-37 (IL-37) has been identified as a novel anti-inflammatory cytokine. The present study aimed to evaluate the expression of IL-37 in serum and in peritoneal fluid to determine its clinical significance in endometriosis. Enzyme-linked immunosorbent assay (ELISA) was performed to examine serum IL-37 levels i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.03.012
更新日期:2017-05-01 00:00:00
abstract::The monocytic cell line I937 was derived from U937 by sorting for cells with high expression of MHC class II molecules. Further analysis of these class II molecules revealed the presence of the HLA-DR3 subtype suggesting that the cell line was a potential candidate for testing antigen presentation to T cells restricte...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)02478-6
更新日期:1996-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.10.018
更新日期:2006-02-28 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.06.001
更新日期:2006-08-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90126-0
更新日期:1989-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.08.005
更新日期:2014-11-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(00)00285-6
更新日期:2000-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.10.015
更新日期:2006-03-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90084-8
更新日期:1987-01-01 00:00:00
abstract::The aim of the present work was to develop an in vitro model of eosinophil cytotoxicity which mimics numerous in vivo situations characterized by complement activation through the alternative pathway. Our results demonstrate that eosinophilic granulocytes from patients with parasitic diseases and blood eosinophilia we...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90078-7
更新日期:1986-01-01 00:00:00
abstract::The effects of cilostazol on stimulating heme oxygenase (HO)-1 expression including signal pathways and suppression of inflammatory cytokines and molecules were studied. Cilostazol stimulation time (1-8 h)- and concentration (1-30 μM)-dependently increased the HO-1 mRNA and protein expression associated with increased...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2011.01.003
更新日期:2011-05-01 00:00:00
abstract::Yolkin is a product of proteolytic degradation of vitellogenin, a protein contained in eggs' yolk, with already described procognitive properties. Here, we investigated effects of yolkin on the humoral and cellular immune response in mice, phenotype of cells from lymphoid organs and function of innate immunity cells. ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.01.003
更新日期:2020-04-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.04.008
更新日期:2014-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90091-f
更新日期:1993-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.12.019
更新日期:2010-02-16 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(96)02675-2
更新日期:1996-12-01 00:00:00
abstract::An effective HIV vaccine will likely need to induce potent and broad-based immunity, including CD8+ T cell responses. Hence, a quantitative assay to measure such responses in animal models will be important in the evaluation of candidate HIV vaccines. We show here that a single immunization with HIV DNA vaccines, foll...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00237-7
更新日期:2003-01-22 00:00:00