Abstract:
:Thymocyte conditioned medium (TCM) was prepared using 2 lots of fetal calf, and 1 lot each of horse and rabbit sera. The 4 TCM and the respective sera alone were compared in their ability to support primary in vitro sensitization to sheep red blood cells (SRBC) as assayed by direct plaque formation. As would be expected, only the fetal calf sera (FCS) supported the primary in vitro sensitization to SRBC, while the use of the TCM prepared from the FCS resulted in a doubling of direct plaque formation. TCM prepared from either the rabbit or horse sera did not provide adequate support for this primary antibody response to SRBC. These data indicate that only sera able to support primary in vitro sensitization is useful for the preparation of active TCM ultimately used in B cell sensitization for hybridoma formation.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
White LL,Smith RAdoi
10.1016/0165-2478(87)90084-8subject
Has Abstractpub_date
1987-01-01 00:00:00pages
87-9issue
2eissn
0165-2478issn
1879-0542pii
0165-2478(87)90084-8journal_volume
14pub_type
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更新日期:2007-08-15 00:00:00
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:1990-06-01 00:00:00
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journal_title:Immunology letters
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更新日期:2004-04-15 00:00:00
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journal_title:Immunology letters
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更新日期:2016-10-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2003-01-02 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(03)00029-4
更新日期:2003-05-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.11.004
更新日期:2007-01-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.04.008
更新日期:2014-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)00163-4
更新日期:1994-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2011.03.010
更新日期:2011-08-30 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90004-5
更新日期:1985-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90040-8
更新日期:1983-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90029-3
更新日期:1982-03-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2018.01.004
更新日期:2018-04-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2004.08.018
更新日期:2005-01-31 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.12.011
更新日期:2007-03-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90068-8
更新日期:1983-06-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.06.004
更新日期:2013-06-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.02.011
更新日期:2005-07-15 00:00:00
abstract::Recombinant and pure "natural" IL-1 and IL-2 were compared with the muramyl dipeptide (MDP) component of Freund's adjuvant for their capacity to enhance the humoral immune response against foot-and-mouth disease (FMD) virus antigen. Using a dose of this antigen which alone did not give a detectable immune response, an...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90008-c
更新日期:1992-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2011.01.010
更新日期:2011-05-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90178-6
更新日期:1985-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00059-e
更新日期:1995-07-01 00:00:00