Abstract：
:Humanized mouse models present an important tool for preclinical evaluation of new vaccines and therapeutics. Here we show the human variable repertoire of antibody sequences cloned from a previously described human immune system (HIS) mouse model that possesses functional human CD4+ T cells and B cells, namely HIS-CD4/B mice. We sequenced variable IgG genes from single memory B-cell and plasma-cell sorted from splenocytes or whole blood lymphocytes of HIS-CD4/B mice that were vaccinated with a human plasmodial antigen, a recombinant Plasmodium falciparum circumsporozoite protein (rPfCSP). We demonstrate that rPfCSP immunization triggers a diverse B-cell IgG repertoire composed of various human VH family genes and distinct V(D)J recombinations that constitute diverse CDR3 sequences similar to humans, although low hypermutated sequences were generated. These results demonstrate the substantial genetic diversity of responding human B cells of HIS-CD4/B mice and their capacity to mount human IgG class-switched antibody response upon vaccination.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Nogueira RT,Sahi V,Huang J,Tsuji Mdoi
10.1016/j.imlet.2017.06.001subject
Has Abstractpub_date
2017-08-01 00:00:00pages
46-52eissn
0165-2478issn
1879-0542pii
S0165-2478(17)30100-1journal_volume
188pub_type
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