Abstract:
:The aim of this work was to raise allotype specific monoclonal antibodies to human CD45, with the long-term objective of producing a reagent which could be used to prolong graft survival in renal transplantation through removal of passenger leukocytes from the graft. At present there are no anti-CD45 monoclonal antibodies able to distinguish between host and donor leukocytes. An in vitro immunisation technique has been developed through which donated human leukocytes are sensitised to CD45 prior to fusion with a myeloma cell line. IgM was produced by all the anti-CD45-positive clones. Flow cytometric analysis using these antibodies showed their ability to differentiate between blood from individual donors, indicating the existence of allotypic forms of human CD45, in conformity with the findings in rats and pigs. Therefore, a reagent which could be used in renal transplantation is a technical possibility.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Dean GS,Aspinall Rdoi
10.1016/s0165-2478(99)00056-5subject
Has Abstractpub_date
1999-06-01 00:00:00pages
333-7issue
2-3eissn
0165-2478issn
1879-0542pii
S0165-2478(99)00056-5journal_volume
68pub_type
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更新日期:1994-06-01 00:00:00
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journal_title:Immunology letters
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更新日期:1990-10-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90056-7
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journal_title:Immunology letters
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更新日期:1996-05-01 00:00:00
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journal_title:Immunology letters
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0165-2478(91)90125-t
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pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90091-9
更新日期:1984-01-01 00:00:00
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pub_type: 杂志文章
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