Abstract:
:Seroma is a frequent complication of breast cancer surgery, the etiology of which remains indefinite. It represents a subcutaneous accumulation of fluid frequently reported after surgical procedures such as axillary lymph node dissection. Despite previous studies have associated seroma fluid to an inflammatory exudate, the surgical removal of draining lymph nodes may indicate that seroma might not represent a mere exudate but rather an accrual of lymph drained from tributary tissues. To verify this hypothesis, seromas were collected at different intervals of time in patients operated upon for axillary lymph node removal. Fluids were analyzed in details by flow cytometry and biochemical assays for their cellular content and for their molecular features and relevant cytokine content. Lymphocytes and other peculiar blood mononuclear cells were present, while erythrocytes, platelets and granulocytes were absent or extremely rare. The protein concentration resulted lower (median 64%) than in peripheral blood. However, specific proteins related to locoregional tissues resulted highly concentrated (e.g. up to 500% for ferritin and 300% for lactate deydrogenase and exclusive presence of interleukin-6) whereas all enzymes and proteins synthesized in the liver or other organs (e.g. alkaline phosphatase, ALT, gammaGT, prealbumin, transferrin, ceruloplasmin, C3 and C4, alpha2 macroglobulin from liver; apolipoproteins from liver and gut; amylase and lipase from pancreas) were represented in reduced concentrations, thus ruling out that seroma proteins derive directly from blood serum. As a whole, this comprehensive cytological and molecular analysis provided evidences that seroma is constituted by serum ultrafiltrated-derived extracellular fluid of regions located upstream of removed lymph nodes. This fluid is then enriched by proteins and cells collected in the drained regions. Remarkably, seroma fluids collected in the same patient at different time points (up to 50 days following surgery) displayed similar biochemical features, clearly indicating that fluid composition was not significantly affected by post-surgical locoregional flogosis. Finally, the period of seroma formation indicates that lymph accumulates in the axillary region during the interval of time needed for afferent lymphatic vessels to re-anastomose with the efferent ducts. Therefore, seroma fluid represents a font of biological material suitable for investigating the biology of breast cancer, healing tissues and lymph.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Montalto E,Mangraviti S,Costa G,Carrega P,Morandi B,Pezzino G,Bonaccorsi I,Cancellieri A,Mingari MC,Mesiti M,Ferlazzo G,Melioli Gdoi
10.1016/j.imlet.2010.03.002subject
Has Abstractpub_date
2010-06-15 00:00:00pages
67-72issue
1eissn
0165-2478issn
1879-0542pii
S0165-2478(10)00093-3journal_volume
131pub_type
杂志文章abstract::To study the mechanism(s) responsible for the appearance of Epstein-Barr virus (EBV)-induced anti-histone autoantibodies, peripheral blood B lymphocytes from healthy donors were infected with EBV and the resulting lymphoblastoid cell lines were tested for secretion of antibodies reacting with histones. It was found th...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90169-4
更新日期:1994-03-01 00:00:00
abstract::The leukocyte common antigen, CD45, is one of the major glycoproteins on cells of hemopoietic origin showing considerable heterogeneity in both structure and expression. Biochemical heterogeneity has been attributed to differences in the primary sequence and glycosylation. In this paper we report an additional basis f...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90198-8
更新日期:1994-04-01 00:00:00
abstract::A theoretical analysis of experimental data is presented in this mini-review on non-random homo- and hetero-associations of cell surface receptors, which can be recruited in the plasma membrane or at the surface of the rough endoplasmic reticulum during the protein synthesis. In the latter case, the likely genetic ori...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(02)00024-x
更新日期:2002-06-03 00:00:00
abstract::Murine peritoneal macrophages were isolated by adherence and their listericidal activity assessed in the presence or absence of selected cytokines. Untreated macrophages were not highly listericidal, showing moderate killing in the first 2 h after infection, and allowed progressive microbial growth thereafter (up to 9...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90158-7
更新日期:1991-03-01 00:00:00
abstract::Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria. As such, it signals monocytes, macrophages and neutrophils to up-regulate phagocytic functions and to release pro-inflammatory cytokines. Despite the established role of CD14 as the main LPS receptor, the precise nature of t...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.07.019
更新日期:2005-01-15 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is an attractive target for the treatment of epithelial cancers. However, EGFR antagonists have low clinical response rates and frequently induce resistance mainly caused by the hypermutation of the extracellular and intracellular domains and the heterodimerization of EGFR. Dime...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.10.011
更新日期:2016-12-01 00:00:00
abstract::CD27, which belongs to the TNF receptor family, is a costimulatory molecule that participates in T-cell activation. Unlike costimulatory molecules such as OX40 and 4-1BB, little is known about the role CD27 plays a role in the development of experimental diseases. We asked whether CD27 and its ligand CD70 participate ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.05.004
更新日期:2008-08-15 00:00:00
abstract::We have explored the requirements for the induction of the N-formyl-methionyl-leucyl-phenylalanine (FMLP) response in Daudi cells after anti-immunoglobulin treatment. Our results indicate that (a) induction of responsiveness to FMLP was observed in Daudi only after crosslinking of surface immunoglobulin by anti-immuno...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90110-a
更新日期:1992-04-01 00:00:00
abstract::Donor-specific blood transfusion (DST) improves allograft survival, although the exact mechanism(s) is not completely understood. The purpose of this study was to determine the role of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) in DST-protective effects, using a rat cardiac transplantation model. Lewis rats (RT...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00086-x
更新日期:2002-09-02 00:00:00
abstract::It is well-established that chemokines play a critical role in the orchestration of inflammation and immunity. Interactions between chemokines and their receptors are essential for the homing of specific subsets of leukocytes to their functional microenvironments. They also influence other diverse biological processes...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2004.08.018
更新日期:2005-01-31 00:00:00
abstract::When the random polymer (Glu, Phe)n was added to primary lymph node T cell cultures from mice immunized with (Glu, Lys, Ala) and other immunogens, significant inhibition of [3H]thymidine incorporation was noted. A similar addition of (Glu, Phe)n to murine T cell lines (Fathman) from the above reacting lymph nodes led ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90160-x
更新日期:1987-12-01 00:00:00
abstract::We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90155-1
更新日期:1988-12-01 00:00:00
abstract::We investigated IgE-mediated allergic responses in a metabolic syndrome model rat strain, SHRSP.Z, which develops obesity and hypertension to cast light on the relationship between metabolic disturbances and allergic responses. IgE-mediated cutaneous anaphylactic responses were severely attenuated in this strain regar...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.11.007
更新日期:2010-01-18 00:00:00
abstract::There is increasing evidence for the role of basophils in allergic bronchial asthma. We studied the potential role of basophils in the pathogenesis of post-exercise-induced bronchoconstriction by measuring the histamine release from basophils both spontaneously and following ConA, FMLP, anti-IgE and TPA treatment. Two...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90026-4
更新日期:1994-09-01 00:00:00
abstract::N-Acetyl-l-cysteine (NAC) is a well-known medication, primarily used as a mucolytic agent in pulmonary disease. Recently, we have found that NAC possesses antipyretic properties. The aim of the present study was to investigate the mechanism by which NAC attenuates fever. The concentration of interleukin (IL)-10 and pr...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.06.005
更新日期:2016-09-01 00:00:00
abstract::In this study the effect of PHA activation on the phosphatase activity of CD45 has been investigated in human leukemic T-cell lines. It has been found that in vivo activation of the cells with PHA resulted in 2-4-fold increase in enzyme activity. Addition of PHA to the postnuclear supernatant of cell lysates also resu...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90086-8
更新日期:1994-10-01 00:00:00
abstract::Isolated inherited deficiency states of almost every complement protein have been recognized. Almost all are autosomal recessive traits. Deficiency of the early-acting components C1, C4 and C2 is associated with increased risk of immune complex disease, particularly systemic lupus erythematosus. Patients with deficien...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90098-8
更新日期:1987-02-01 00:00:00
abstract::The three-dimensional structures of cyclosporin A complexed with cyclophilin A or Fab fragment of a monoclonal antibody were compared. In these two complexes conformations of the fragment D-alanine8-N-methylvaline11 in cyclosporin A were in a good agreement. In addition, cyclophilin A and the Fab fragment had related ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90135-x
更新日期:1994-07-01 00:00:00
abstract::To examine whether grape seed proanthocyanidin extract (GSPE) which is known to act as an antioxidant has therapeutic effect on collagen-induced arthritis (CIA) in mice, an animal model of rheumatoid arthritis. Mice were treated with an intraperitoneal injection of GSPE (10, 50, or 100 mg/kg) or saline. Clinical, hist...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.05.001
更新日期:2009-06-04 00:00:00
abstract::Genetic or DNA-based immunization, including genomic immunization, has shown to be a viable alternative approach to induce protective immunity against a number of pathogens in several disease models. Here we describe a new method, cDNA expression library immunization (cDELI), based on the use of a large number of cDNA...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00039-x
更新日期:1998-07-01 00:00:00
abstract::Antisera were raised in rabbits against a monoclonal antibody (McAb 103) of C3H.SW origin which is specific to the synthetic polypeptide (T,G)-A-L and was shown to express the major idiotypic determinants of conventional anti-(T,G)-A-L antibodies. Antibodies were purified and were shown in a binding assay to recognize...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90130-0
更新日期:1985-01-01 00:00:00
abstract::When dimerized by Stem Cell Factor (SCF), the Receptor Tyrosine Kinase Kit triggers the proliferation of hematopoietic progenitors, including pro-B cells, and of some differentiated cells, including mast cells. We found previously that anti-Kit antibodies can mimic SCF and that anti-Kit-induced mast cell proliferation...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2012.02.007
更新日期:2012-03-30 00:00:00
abstract::Domain deleted paraproteins are suitable tools to study the interaction between IgG domains and Fc receptor (FcR) binding sites. The effect of the C gamma 2 or C gamma 3 domain deleted paraproteins was compared on antibody dependent cellular cytotoxicity (ADCC) and on FcR mediated rosette formation. The C gamma 2 doma...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90035-0
更新日期:1986-06-01 00:00:00
abstract::Dendritic cell (DC)-based tumor vaccine is a promising therapy for malignancies. Recent studies showed greater potency with DC/tumor fusion vaccines against acute myeloid leukemia and melanoma compared with lysate-pulsed DC vaccines. We compared these two vaccine strategies against murine colon cancer and investigated...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.05.006
更新日期:2005-11-15 00:00:00
abstract::Adrenaline has been shown to inhibit the release of tumor necrosis factor (TNF) elicited by lipopolysaccharide (LPS) when tested in vitro on cultured human blood cells and rat macrophages. In this report we have examined the effect of the in vivo administration of adrenaline on TNF serum levels induced by LPS. In agre...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90177-4
更新日期:1993-10-01 00:00:00
abstract::Monoclonal antibodies of the Orthoclone series were used to identify total T lymphocytes (OKT3) and their helper-inducer (OKT4) and cytotoxic-suppressor (OKT8) subsets in 25 patients with chronic Chagas' disease and 25 healthy controls. No significant difference in the number of total T cells (OKT3+) circulating in th...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90011-7
更新日期:1984-01-01 00:00:00
abstract::This study was to investigate the role of macrophage migration inhibitory factor (MIF) in mouse acute otitis media (AOM), we hypothesize that blocking MIF activity will relieve mouse AOM. A mouse AOM model was constructed by injecting lipopolysaccharide (LPS) into the middle ear of C57BL/6 mice through the tympanic me...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.07.013
更新日期:2014-11-01 00:00:00
abstract::Numerous studies have dealt with occurrence of dendritic cells in various nonlymphoid organs such as kidney, liver or heart, whereas lymphocyte patterns in these organs have not been analyzed in detail. In the present study, leukocytes were quantified as cells/mm2 in the perivascular, interstitial and parenchymal tiss...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(96)02595-3
更新日期:1996-11-01 00:00:00
abstract::Previous studies have demonstrated that culture supernatants of LPS-stimulated murine splenic B lymphocytes (BLPSSN) are able to inhibit the growth of freshly isolated B cells via an IgM antibody. In this work we investigated the progress of LPS-activated B lymphocytes through the cell cycle in the presence of this an...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90070-5
更新日期:1992-08-01 00:00:00
abstract::During their differentiation, thymocytes are subjected to two rounds of selection. First, CD4-8- double-negative (DN) thymocytes with a functional TCR-beta chain express a alpha-beta+ CD3 complex on their surface and, as a consequence, are selected to mature to the CD4+8+ double-positive (DP) stage. This round ends af...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)00197-y
更新日期:1995-01-01 00:00:00