Abstract：
:Epidermal growth factor receptor (EGFR) is an attractive target for the treatment of epithelial cancers. However, EGFR antagonists have low clinical response rates and frequently induce resistance mainly caused by the hypermutation of the extracellular and intracellular domains and the heterodimerization of EGFR. Dimerization plays a key role in the activation of the EGFR family of receptors. Thus, targeting the highly conserved dimer interface of EGFR maybe an attractive strategy for improving the clinical response of anti-EGFR therapies. In this work, we report a monoclonal antibody (mAb) called Antidimer 5G9 that targets the β-hairpin within the dimer interface of EGFR, which is 100% homologous with mouse EGFR. Antidimer 5G9 belongs to the isotype IgG1 and bound with an affinity constant of 1.837×109L/mol. Just as designed and expected, Antidimer 5G9 could recognize and bind to human EGFRs or mouse EGFRs on the surface of human breast cancer cells of the MDA-MB-231 cell line or mouse Lewis lung cancer cells (LLC), effectively inhibiting growth and inducing apoptosis in the targeted cells. This work lays the foundation for further studies investigating the feasibility of this strategy for targeting the dimer interface of EGFR and developing novel anti-EGFR drugs.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Guo T,Zhao L,Zhang Y,Liu G,Yao Y,Li Hdoi
10.1016/j.imlet.2016.10.011subject
Has Abstractpub_date
2016-12-01 00:00:00pages
39-45eissn
0165-2478issn
1879-0542pii
S0165-2478(16)30250-4journal_volume
180pub_type
杂志文章abstract：:The thymus contains a low frequency of B cells, about 0.1-0.5% of total population of thymocytes that were shown to contribute in thymic negative selection. The fact that B cells in the periphery have been contributed in the generation of the T regulatory cell compartment emerged the idea that this process may indeed ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.03.007
更新日期：2017-05-01 00:00:00
abstract：BACKGROUND:Interleukin 10 (IL-10) is a cytokine with potent anti-inflammatory properties that play a fundamental role in restrictive host immune response to pathogens, by means of that is a crucial importance for chronic inflammatory disease studies. Therefore, the goal of this study was to measure the correlation of t...
journal_title：Immunology letters
pub_type： 临床试验,杂志文章
doi：10.1016/j.imlet.2017.12.008
更新日期：2018-02-01 00:00:00
abstract：:A majority of human immunodeficiency virus type 1 (HIV-1) infected individuals display a rapid loss of CD4+ lymphocytes with fast progression towards overt acquired immunodeficiency syndrome (AIDS). However, a small proportion of individuals infected by HIV-1 remain immunologically intact for many years. In order to i...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02550-3
更新日期：1996-06-01 00:00:00
abstract：:In some animal models of organ transplantation, notably the rat, preoperative administration of donor strain blood may produce long-term allograft survival even in the absence of adjunctive immunosuppressive therapy. The ability of blood transfusion to prevent rejection is highly dependent on the strain combination an...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(91)90213-t
更新日期：1991-07-01 00:00:00
abstract：:The aim of the present work was to develop an in vitro model of eosinophil cytotoxicity which mimics numerous in vivo situations characterized by complement activation through the alternative pathway. Our results demonstrate that eosinophilic granulocytes from patients with parasitic diseases and blood eosinophilia we...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(86)90078-7
更新日期：1986-01-01 00:00:00
abstract：:Work done in our laboratories, using a murine model, indicates that suppression of host immune responses might be due to secretion of soluble factors by tumor cells. The H238 cells (BALB/c embryonic fibroblasts transformed by UV-inactivated herpes simplex virus Type 2) exhibit progressive tumor growth with subsequent ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90087-q
更新日期：1991-09-01 00:00:00
abstract：:The photobleaching energy transfer (pbFRET) technique is a fluorescence method to measure proximity relationships between molecules, especially cell surface proteins, labeled with fluorophore-conjugated monoclonal antibodies, on a pixel-by-pixel base using digital imaging microscopy. This technique enables analysis of...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(96)02665-x
更新日期：1996-12-01 00:00:00
abstract：:Blood coagulation and inflammation pathways are linked in many aspects. A number of serum factors involved in coagulation cascades affect directly or indirectly inflammatory responses, whereas proinflammatory cytokines influence blood coagulation pathways. In this work we demonstrated that thrombin is an effective sti...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00015-2
更新日期：1999-04-15 00:00:00
abstract：OBJECTIVES:To investigate the associations of cytochrome p450 1A1 (CYP1A1) and manganese superoxide dismutase (MnSOD) genes polymorphisms with the susceptibility to AS in Taiwan. METHODS:The polymorphisms of CYP1A1 and MnSOD genes were determined in 70 patients with ankylosing spondylitis (AS) and 93 healthy controls ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(03)00071-3
更新日期：2003-08-05 00:00:00
abstract：:This article has been withdrawn at the request of the editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. ...
journal_title：Immunology letters
pub_type： 撤回出版物
doi：10.1016/j.imlet.2017.08.015
更新日期：2017-08-26 00:00:00
abstract：:Peripheral blood from 11 newly diagnosed patients with insulin-dependent diabetes mellitus (IDDM) was studied for the proportion of monoclonal antibody (HNK 1, Leu 7) defined natural killer (NK) cells using a fluorescence-activated cell sorter analyzer. The proportion of Leu 7+ cells in patients with IDDM (7.0 +/- 4.0...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90056-7
更新日期：1984-01-01 00:00:00
abstract：:Seroma is a frequent complication of breast cancer surgery, the etiology of which remains indefinite. It represents a subcutaneous accumulation of fluid frequently reported after surgical procedures such as axillary lymph node dissection. Despite previous studies have associated seroma fluid to an inflammatory exudate...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2010.03.002
更新日期：2010-06-15 00:00:00
abstract：:The murine pre-B-like cell line, 70Z /3, has been shown to undergo differentiation in response to supernatants derived from concanavalin-A (Con A) stimulated rat spleen cells or ultraviolet light treated P388D1 macrophage cell line. After culture for 24 h with supernatant factors, normally membrane IgM negative 70Z /3...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90085-3
更新日期：1984-01-01 00:00:00
abstract：:The regulatory effect of Z-100 on the balance of Th1/Th2 cell responses in BALB/c mice bearing Meth-A fibrosarcoma was investigated. In tumor bearing mice, Th1 cytokine production (IL-2, IFN-gamma) are suppressed and Th2 cytokine production (IL-4, IL-10) are increased, as compared with those of normal mice. The admini...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00130-3
更新日期：1999-11-01 00:00:00
abstract：:Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this translocation predicates the subsequent production of IL-2, the me...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2012.01.001
更新日期：2012-02-29 00:00:00
abstract：:Growth is coupled to physiological modifications of the immune system which reaches the functional capabilities according to age-related milestones. Few data are available on the circulating immunoglobulin levels and no data exist on total immunoglobulin light chains in infant macaques. Therefore we studied by a nephe...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02522-9
更新日期：1996-05-01 00:00:00
abstract：:Phagolysis of apoptotic eosinophils plays an important role in the successful resolution of asthmatic inflammation. However, little is known about underlying mechanisms. Our aim is to investigate whether annexin I is involved in the dexamethasone-mediated enhancement of phagolysis of apoptotic eosinophils by A549 cell...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2007.06.002
更新日期：2007-08-15 00:00:00
abstract：:While cases of silicosis are often complicated by various autoimmune disorders, patients with asbestosis develop malignant tumors such as lung cancer and malignant mesothelioma. These differences may derive from different biological effects, particularly on immunological cells, of silica and asbestos. To find differen...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.11.005
更新日期：2005-04-15 00:00:00
abstract：:The first step in the clearance of apoptotic cells is chemotactic migration of macrophages towards the apoptotic cells guided by find-me signals provided by the dying cells. Upon sensing the chemotactic signals, macrophages release ATP. ATP is then degraded to ADP, AMP and adenosine to trigger purinergic receptors con...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.02.002
更新日期：2017-03-01 00:00:00
abstract：:We have previously reported the production of a series of human monoclonal antibodies reacting with mouse mammary tumor viral antigens as well as the human counterpart HuMTV antigen. Against two of these antibodies (B11 and 4.6/6), anti-idiotypic antibodies were generated, which appeared to be the internal image of vi...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90128-w
更新日期：1991-04-01 00:00:00
abstract：:Tuberculosis (TB) is a highly infectious disease and its early and precise diagnosis is essential to reduce morbidity and mortality of patients. Since the routine diagnostic tests (like Monteux, AFB smear microscopy, chest X-Ray) do not give infallible results, additional tests are always recommended. Therefore to add...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2019.03.010
更新日期：2019-05-01 00:00:00
abstract：:In this study the effect of PHA activation on the phosphatase activity of CD45 has been investigated in human leukemic T-cell lines. It has been found that in vivo activation of the cells with PHA resulted in 2-4-fold increase in enzyme activity. Addition of PHA to the postnuclear supernatant of cell lysates also resu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90086-8
更新日期：1994-10-01 00:00:00
abstract：:Malaria vaccine development has traditionally concentrated on careful molecular, biochemical, and immunological characterisation of candidate antigens. In contrast, evidence of the importance of identified antigens in immunity to human infection and disease has generally been limited to statistically significant co-va...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/s0165-2478(02)00159-1
更新日期：2002-11-01 00:00:00
abstract：:N-Acetyl-l-cysteine (NAC) is a well-known medication, primarily used as a mucolytic agent in pulmonary disease. Recently, we have found that NAC possesses antipyretic properties. The aim of the present study was to investigate the mechanism by which NAC attenuates fever. The concentration of interleukin (IL)-10 and pr...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.06.005
更新日期：2016-09-01 00:00:00
abstract：:Monoclonal antibodies (mAb) reacting with native (TA5, TB12) and denatured (T33, T35) plant toxin mistletoe lectin I (MLI) from Viscum album have been obtained. The interaction between mAbs and native toxin with ML isoforms (MLII, MLIII) has been investigated. An immunological cross-reaction has been shown to take pla...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)00181-p
更新日期：1995-01-01 00:00:00
abstract：:Cytokine induced killer (CIK) cells have a powerful tumor cells killing activity both in vitro and in vivo and transfusion of these cells have become an adjuvant treatment for tumors. CIK cells are induced and amplified from peripheral blood mononuclear cells (PBMCs) with multiple cytokines. As CD4+CD25bri regulatory ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.11.009
更新日期：2017-01-01 00:00:00
abstract：:The leukocyte common antigen, CD45, is one of the major glycoproteins on cells of hemopoietic origin showing considerable heterogeneity in both structure and expression. Biochemical heterogeneity has been attributed to differences in the primary sequence and glycosylation. In this paper we report an additional basis f...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90198-8
更新日期：1994-04-01 00:00:00
abstract：:Dendritic cells (DC), the most potent antigen presenting cells (APC), have been shown able to process apoptotic tumor cells and necrotic tumor cells for antigen presentation. Apoptosis and necrosis are the two common final pathways through which the tumors are killed by chemotherapy or radiation therapy. The tumor cel...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2005.09.010
更新日期：2006-03-15 00:00:00
abstract：:The presence of dendritic cells (DCs) in mycobacterium-containing granulomas, as well as in other granuloma-inducing diseases, is beginning to be appreciated. This review will summarize what is known about DCs with regards to the granuloma and discuss the potential roles DCs may be playing during mycobacterial infecti...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2009.12.009
更新日期：2010-05-04 00:00:00
abstract：:Numerous studies have dealt with occurrence of dendritic cells in various nonlymphoid organs such as kidney, liver or heart, whereas lymphocyte patterns in these organs have not been analyzed in detail. In the present study, leukocytes were quantified as cells/mm2 in the perivascular, interstitial and parenchymal tiss...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(96)02595-3
更新日期：1996-11-01 00:00:00
