Abstract:
:In this study the effect of PHA activation on the phosphatase activity of CD45 has been investigated in human leukemic T-cell lines. It has been found that in vivo activation of the cells with PHA resulted in 2-4-fold increase in enzyme activity. Addition of PHA to the postnuclear supernatant of cell lysates also resulted in elevation of phosphatase activity. Elevation of enzyme activity resulted from an increase in the amount of antigen in the immunoprecipitates. Elevation of the quantity was not the result of a de novo protein synthesis since the presence of cycloheximide, a protein synthesis inhibitor, did not modulate the effect of PHA. The effect of PHA was specific since ConA, that also bound to the CD45 molecules, or crosslinking of the antigen by antibody did not affect CD45. Since direct binding of PHA to CD45 molecules was shown in immunoblotting analysis, we suggest that the effect of PHA is a consequence of a PHA-induced conformational change of CD45 that results in up-regulation of the analyzed CD45 epitopes.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Monostori E,Hartyani Z,Ocsovszky I,Hegedus Z,Oravecz T,Kalman M,Ando Idoi
10.1016/0165-2478(94)90086-8subject
Has Abstractpub_date
1994-10-01 00:00:00pages
197-201issue
3eissn
0165-2478issn
1879-0542pii
0165-2478(94)90086-8journal_volume
42pub_type
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