Abstract：
:DNA-dependent RNA polymerase was partially purified from wheat germ extract and tested for inhibition by antinuclear autoantibodies from the sera of patients with connective tissue diseases. The enzyme was inhibited by anti-DNA and by autoantibodies to the nuclear ribonucleoprotein nRNP. Autoantibodies to other ribonucleoproteins (Sm, Ro, La) did not cause inhibition. The enzyme preparation was shown to contain material with Ro and La antigenic activity but there was no nRNP or Sm detectable by immune precipitation. Previous work [3] has shown inhibition of prokaryotic (E. coli) RNA polymerase by anti-DNA, and our results show that the eukaryotic enzyme, in this case from wheat germ, is also inhibited. The results are consistent with the suggestion that inhibition by anti-DNA is due to template masking. Inhibition of RNA polymerase by antibodies to cellular ribonucleoprotein suggests that the antigen is in some way associated with the activity of the enzyme.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Hough DW,Miller NI,Maddison PJdoi
10.1016/0165-2478(83)90093-7subject
Has Abstractpub_date
1983-03-01 00:00:00pages
123-7issue
3eissn
0165-2478issn
1879-0542pii
0165-2478(83)90093-7journal_volume
6pub_type
杂志文章abstract：:Immunoglobulin species, recombined from Fab and Fc fragments with anti-red cell specificity were used as cytophilic antibodies in studies of triggering the effector activity of immunoglobulins. Recombinant immunoglobulins containing a single Fab' appeared inactive in binding to cell receptors and triggering antibody d...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90046-4
更新日期：1984-01-01 00:00:00
abstract：:The complement system is a protein cascade capable of neutralizing invading pathogens. One of its activation pathways is the lectin pathway which is dependent on the binding of MBL or the ficolins. The specificity of L-ficolin binding has been investigated previously and it was observed that binding is dependent on ac...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2008.03.014
更新日期：2008-06-30 00:00:00
abstract：:The link between innate and adaptive host immune response to Mycobacterium tuberculosis (M.tb) is driven by dendritic cells (DC). In this study, we examined the ability of prevalent clinical strains from south India (S7, S10) and laboratory strain H37Rv (Rv) to induce maturation of monocyte derived dendritic cells (Mo...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2008.01.009
更新日期：2008-05-15 00:00:00
abstract：:The effects of recombinant human interleukin 4 (rIL-4) on proliferation and differentiation on human myeloid/monocytic leukemia cell lines were examined. At high concentrations, rIL-4 had a slight enhancing effect on [3H]thymidine incorporation by U937 cells. rIL-4 markedly induced expression of the Fc epsilon recepto...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(89)90027-8
更新日期：1989-06-15 00:00:00
abstract：:It is well known that most physiological functions change with aging, including the immune response. Data concerning the aging of lymphocyte subpopulations are conflicting. The antigen density of peripheral blood lymphocytes has been determined by fluorescently tagged OKT-3, OKT-4, OKT-8, OKT-11 and OKM1 monoclonal an...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90072-x
更新日期：1990-02-01 00:00:00
abstract：:It has been considered before that human naive and memory/effector CD4+ T-cells cannot be subdivided solely according to the differential expression of CD45 isoforms. By the lack of expression of CD31 we have identified a subset of CD4+ CD45RA+ CD31- cells which show distinct features of antigen-experienced Th1 cells....
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00056-4
更新日期：1997-06-01 00:00:00
abstract：:Phosphatidylinositol 3-kinase (PI3K) is critical player in cell proliferation and survival. The effects of LY294002 and wortmannin, inhibitors of PI3K, on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipoploysaccharide (LPS)-induced Raw 264.7 cells were investigated. Significan...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.12.007
更新日期：2005-06-15 00:00:00
abstract：BACKGROUND:Dendritic cells (DCs) are professional antigen-presenting cells that are capable of inducing immune responses. DC-based vaccines are normally generated using a standard 5- to 7-day protocol. To shorten the DC-based vaccine production for use in cancer immunotherapy, we have developed a fast DC protocol by co...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.03.010
更新日期：2014-07-01 00:00:00
abstract：:This article has been withdrawn at the request of the editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. ...
journal_title：Immunology letters
pub_type： 撤回出版物
doi：10.1016/j.imlet.2017.08.015
更新日期：2017-08-26 00:00:00
abstract：:In murine models, challenge with different viral and parasitic infection is closely associated with the production of type I interferons (IFN-alpha/beta) and NK cell production of interferon-gamma (IFN-gamma). Therefore, we wished to determine if IFN-alpha/beta had a role in the regulation of NK cell production of IFN...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00091-6
更新日期：1997-10-01 00:00:00
abstract：:The cellular source of murine LAIF detected by an indirect leukocyte adherence inhibition (LAI) assay using capillary tubes was investigated using non-inducing peritoneal cells (PC) from normal and spindle-cell sarcoma-bearing A/J mice. Cell populations containing greater than 95% T-cells, B-cells or macrophages were ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(81)90005-5
更新日期：1981-11-01 00:00:00
abstract：:Multiple sclerosis (MS) is a debilitating neurological disorder of the central nervous system (CNS), characterized by activation and infiltration of leukocytes and dendritic cells into the CNS. In the initial phase of MS and its animal model, experimental autoimmune encephalomyelitis (EAE), peripheral macrophages infi...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2014.03.006
更新日期：2014-07-01 00:00:00
abstract：:Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(92)90193-r
更新日期：1992-03-01 00:00:00
abstract：:Work done in our laboratories, using a murine model, indicates that suppression of host immune responses might be due to secretion of soluble factors by tumor cells. The H238 cells (BALB/c embryonic fibroblasts transformed by UV-inactivated herpes simplex virus Type 2) exhibit progressive tumor growth with subsequent ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90087-q
更新日期：1991-09-01 00:00:00
abstract：:Interleukin (IL)-17A is a cytokine that plays an important role in infectious, autoimmune, and inflammatory diseases. In this study, we found that TCRγδ(+)CD4(-)CD8(-) T cells, but not TCRαβ(+)CD4(+) T cells, are the primary producers of IL-17A in the genital tract of female mice in the steady-state condition. High mR...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2012.05.006
更新日期：2012-09-01 00:00:00
abstract：:Sepsis is an abnormal immune response to infection characterized by an overwhelming systemic inflammation and cell death. Non-apoptotic cell death pertaining to pyroptosis, necroptosis and autophagy contribute to sepsis pathogenesis apart from classical apoptotic cell death. The objective of the current study is to in...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2020.12.009
更新日期：2021-01-03 00:00:00
abstract：:The carboxy-terminus of the major merozoite surface protein of Plasmodium has been shown to be the target of protective immunity in a number of non-vivax malaria parasite species. In an effort to develop a protective vaccine for Plasmodium vivax, the most prevalent form of human malaria, we expressed in Saccharomyces ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02570-9
更新日期：1996-07-01 00:00:00
abstract：:The effect of priming with various antigens on subsequent vaccination with the pneumococcal conjugate vaccine (CPV) was determined using BALB/c mice. Priming with pneumococcal polysaccharide or cross-reactive polysaccharide did not inhibit the IgG response to CPV immunization. Additionally, live intranasal colonizatio...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.04.013
更新日期：2006-08-15 00:00:00
abstract：BACKGROUND:Chronic obstructive pulmonary disease (COPD) is a non-specific inflammation, which involves the airways, lung parenchyma and pulmonary vessels. The inflammation causes the activation of inflammatory cells and the release of various inflammatory mediators such as interleukin-1 beta, interleukin-6 and tumor ne...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2018.01.004
更新日期：2018-04-01 00:00:00
abstract：:Lyophilized bacterial lysates, which actively stimulate the immune response, are widely used as vaccines or 'biological response modifiers' in subjects with recurrent bacterial respiratory infections. Since vaccines are indicated in the absence or in the presence of a weak constitutive immune response activity, a bett...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00290-0
更新日期：2003-03-03 00:00:00
abstract：:We have reported that a cell line, KML1, obtained from lymph node cells of a 16-week-old female MRL/Mp-lpr/lpr (MRL/1) mouse produces a soluble factor(s) that promotes formation of anti-single-stranded (ss) DNA antibodies by cultures of spleen cells from old MRL/l mice (greater than 16 weeks old). Detailed examination...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90035-o
更新日期：1990-03-01 00:00:00
abstract：:Thymocyte conditioned medium (TCM) was prepared using 2 lots of fetal calf, and 1 lot each of horse and rabbit sera. The 4 TCM and the respective sera alone were compared in their ability to support primary in vitro sensitization to sheep red blood cells (SRBC) as assayed by direct plaque formation. As would be expect...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(87)90084-8
更新日期：1987-01-01 00:00:00
abstract：:A majority of natural killer (NK) cells constitutively express intermediate affinity IL2 receptors made of beta gamma chains and respond by way of enhanced cytokine secretion. However, alterations in cytokine sensitivity of purified NK cells with respect to cytokine secretion during immunosenescence have not been exam...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02519-9
更新日期：1996-04-01 00:00:00
abstract：:Type 1 diabetes mellitus (T1DM) is a chronic autoimmune disease characterized by the destruction of insulin-secreting β cells upon autoreactive T cell attack. Oral administration of autoantigens is an attractive approach to treating T1DM, but an effective carrier should be used in order to protect antigens. Lactococcu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.04.008
更新日期：2016-06-01 00:00:00
abstract：:Adoptive transfer of multivirus-specific T cell lines (MVST) is an advanced tool for immunotherapy of virus infections after hematopoietic stem cell transplantation (HSCT). Their preparation includes activation of donor virus-specific T cells by the mixture of oligopeptides derived from immunodominant antigens of seve...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2020.09.009
更新日期：2020-12-01 00:00:00
abstract：:Adult T-cell leukemia-derived factor (ADF)/human thioredoxin (TRX) has thiol-dependent reducing activities and is known to have regulatory roles on the DNA-protein interaction and cell activation. Inducive effect of ultraviolet (UV) has been indicated because of the enhanced expression of ADF/TRX in epidermal cells of...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(95)00213-o
更新日期：1995-01-01 00:00:00
abstract：:Growth is coupled to physiological modifications of the immune system which reaches the functional capabilities according to age-related milestones. Few data are available on the circulating immunoglobulin levels and no data exist on total immunoglobulin light chains in infant macaques. Therefore we studied by a nephe...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02522-9
更新日期：1996-05-01 00:00:00
abstract：:Transplantation immunologists have long been intrigued by the natural allograft that results from normal mammalian pregnancy. Its general success contrasts with the rejection problems associated with most artifactual organ transplantation and raises intriguing questions concerning the nature of the mechanisms involved...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(88)90001-6
更新日期：1988-04-01 00:00:00
abstract：:An isogenic pair of virulent and avirulent Yersinia enterocolitica O9 strains was used to examine the influence of the virulence plasmid on the non-specific modification of the cellular immunity in BALB/c mice after experimental infection with yersiniae. The modification of contact hypersensitivity response to dinitro...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(95)02481-6
更新日期：1996-01-01 00:00:00
abstract：:Human Clq, a subcomponent of the first component of complement interacts with human fibronectin. Using ELISA methodology fixation of Clq to solid phase fibronectin, as well as fibronectin to solid phase Clq has been demonstrated. Cl in its native macromolecular form displays little reactivity for fibronectin, nor does...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(82)90075-x
更新日期：1982-01-01 00:00:00
