Abstract:
:Clone C19 derived from a rat bladder cancer cell line BC31ad constitutively released cytotoxic factor RCF. The serum-free C19-conditioned medium was partially purified by DEAE-Sephacel, Sephacryl S-200 and MonoQ-FPLC chromatographies to the specific activity of 6.4 x 10(6) U/mg protein (LM assay). RCF was a 17-kDa protein with pI 4.6-5.0. Anti-RCF serum neutralized the cytotoxic activity of rat and mouse, but not human, tumor necrosis factor-alpha (TNF alpha) as well as RCF. The partial amino acid sequence of RCF was homologous with the sequence 43-58 of rat TNF alpha. RCF and rat TNF alpha showed a similar cytotoxic spectrum against several tumor cells. Furthermore, they induced a marked proliferation of mouse T cells in combination with a suboptimal dose of ConA or anti-CD3 antibody, however, human TNF alpha almost not. Taken together, RCF was substantially regarded as rat TNF alpha.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Hojo H,Kaneko A,Nanya K,Kayagaki N,Hashimoto Ydoi
10.1016/0165-2478(94)90051-5subject
Has Abstractpub_date
1994-06-01 00:00:00pages
19-24issue
1eissn
0165-2478issn
1879-0542pii
0165-2478(94)90051-5journal_volume
41pub_type
杂志文章abstract::Interleukin-18 (IL-18) plays a central role in the immune response by acting on Th1 cell differentiation, cell-mediated cytotoxicity and inflammation. The role of IL-18 in cancers and inflammatory diseases is discussed in the light of our investigations on IL-18 synthesis in normal colonic mucosa, colonic cancer and C...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(00)00285-6
更新日期:2000-12-01 00:00:00
abstract::To test whether apoptotic thymocytes can be identified by altered packing of lipids in their plasma membranes, thymocytes were isolated from mice injected with hydrocortisone and stained with merocyanine 540 (MC540), a fluorescent probe sensitive to lipid packing. At 9-10 h after injection, a subpopulation of H2-Klo c...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90101-7
更新日期:1993-06-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90011-c
更新日期:1992-01-01 00:00:00
abstract::The effect of B cell depletion on the induction and severity of murine experimental autoimmune thyroiditis was investigated. Thirteen CBA mice were given repeated intraperitoneal doses of 700 micrograms purified rabbit anti-mouse Ig antibody from 24 hours to 8 weeks after birth. Controls were given normal rabbit IgG (...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90063-1
更新日期:1989-01-15 00:00:00
abstract::The antigen receptors on the surface of B- and T-lymphocytes are complexes of several integral membrane proteins, essential for their proper expression and function. Recent studies demonstrated that transmembrane (TM) domains of the components of these receptors play a critical role in their association and function. ...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(98)00100-x
更新日期:1998-12-01 00:00:00
abstract::To study the interactions between Chlamydia trachomatis (CT) and human immunodeficiency virus (HIV) infections, we examined CT serologies in sequential serum samples of male homosexuals (MHS), followed over a mean period of 4 years. Of the MHS studied, 77 were HIV(-), 18 were HIV(+) and 10 patients seroconverted durin...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90201-1
更新日期:1994-04-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00034-5
更新日期:1997-07-01 00:00:00
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更新日期:2003-02-03 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2006-05-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.11.016
更新日期:2005-05-15 00:00:00
abstract::Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90193-r
更新日期:1992-03-01 00:00:00
abstract::Recently, we determined the molecular locations on BoNT/A of the antigenic regions recognized by blocking Abs of cervical dystonia patients immunoresistant to BoNT/A treatment. In the present work we tested the possibility of reducing the levels of the Ab response against immunodominant antigenic sites on the heavy ch...
journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2011-06-30 00:00:00
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doi:10.1016/s0165-2478(00)00314-x
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2010.03.002
更新日期:2010-06-15 00:00:00
abstract::Two of the better characterized antimicrobial mechanisms displayed by human neutrophils are the reactive oxygen species (ROS) production and the induction of apoptosis. Their importance in mycobacterial infections is, however, controversial and we aimed to analyze them simultaneously in neutrophils infected with eithe...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.07.006
更新日期:2009-09-22 00:00:00
abstract::The antibody (Ab) response induced by DNA-based immunization was compared in various strains of inbred, H-2 congenic and outbred mice with different haplotypes of mouse major histocompatibility complex (H-2). Two different plasmid expression vectors encoding Aequorea victoria green fluorescent protein (GFP) or Escheri...
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pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00266-2
更新日期:2000-11-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.05.013
更新日期:2005-11-15 00:00:00
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abstract::Human natural killer (NK) cell activity was not significantly affected by leukotriene (LT) B4 over a wide concentration range (10(-6)-10(-14) M), whether added directly to the NK cell assay or after preincubation of effector cells for 2 h with the drug before addition of Cr labelled K562 target cells. In addition, LTB...
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journal_title:Immunology letters
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更新日期:1998-06-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
abstract::CD24 is an extensively glycosylated membrane protein that is linked to the membrane via a glycosyl-phosphatidylinositol (GPI)-anchor. In mice, CD24 is expressed by hematopoietic and non-hematopoietic cells. CD24-/- mice do not have gross immunological defects, but detailed analysis revealed strongly reduced responses ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.06.004
更新日期:2014-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.11.004
更新日期:2007-01-15 00:00:00
abstract::Allogeneic, H-2-incompatible irradiation chimeras (H-2d leads to H-2b) constructed with normal, unmanipulated bone marrow and with marrow-derived factors live long and do not manifest a GvH disease. Their response to primary immunization is deficient but their alloreactivity is normal. This chimeric allotolerance cann...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90003-2
更新日期:1983-04-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00176-5
更新日期:2000-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.07.013
更新日期:2009-09-22 00:00:00
abstract::The production of neutralizing antibodies against the central portion of HIV-1 gp120 requires cooperation between T cell and B cell epitopes of this region. We found a motif common to the carboxyterminus of the second conserved region of HIV-1 gp120 and sequences of some human proteins, most of which participate in im...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90145-g
更新日期:1990-11-01 00:00:00
abstract::Human Clq, a subcomponent of the first component of complement interacts with human fibronectin. Using ELISA methodology fixation of Clq to solid phase fibronectin, as well as fibronectin to solid phase Clq has been demonstrated. Cl in its native macromolecular form displays little reactivity for fibronectin, nor does...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90075-x
更新日期:1982-01-01 00:00:00