Abstract：
AIM:To develop new approach for tumor immunotherapy with VEGFR2-based DNA vaccine. METHODS:VEGFR2 derived from different species were prepared by RT-PCR and subjected to recombinant constructs. The chimeric gene of VEGFR2 was prepared by integrating different epitopes in different species. By transfecting into cells, the immunological effect was evaluated with the approaches of ELISA, ELISPOT, and animal experiments. RESULTS:(1) All these constructs, in particular chimeric construct, can result in both humoral- and cellular-immune response in BALB/c mice after immunization evaluated by the ratio of CD4+/CD8+ and release of IFN-gamma and IL-4 respectively. (2) Mice were vaccinated with these constructs and challenged with renal carcinoma cells subsequently. It manifested that tumor growth was suppressed significantly in the mice vaccinated by chimeric VEGFR2 construct. CONCLUSION:Our results manifested that VEGFR2-based DNA chimeric vaccine could be developed as a promising approach for tumor immunotherapy.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Yan J,Jia R,Song H,Liu Y,Zhang L,Zhang W,Wang Y,Zhu Y,Yu Jdoi
10.1016/j.imlet.2009.07.013subject
Has Abstractpub_date
2009-09-22 00:00:00pages
60-6issue
1-2eissn
0165-2478issn
1879-0542pii
S0165-2478(09)00189-8journal_volume
126pub_type
杂志文章abstract：:Recent data provide evidence that antigen-specific CD4+ T-cell clones or antigen-activated T-cell lines can kill antigen-presenting cells (APC). We focused our studies on monocytes acting as APC in cultures of T cells freshly isolated from peripheral blood. The presence of monocytes in culture was monitored by their a...
journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1995-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：2006-04-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1992-03-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1992-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1987-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：2008-06-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1998-04-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00110-7
更新日期：1997-11-01 00:00:00
abstract：:This article has been withdrawn at the request of the editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. ...
journal_title：Immunology letters
pub_type： 撤回出版物
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更新日期：2017-08-26 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1991-03-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00034-6
更新日期：1999-05-03 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(96)02665-x
更新日期：1996-12-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.06.004
更新日期：2014-09-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(98)00002-9
更新日期：1998-04-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90145-2
更新日期：1985-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2005.10.018
更新日期：2006-02-28 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.08.016
更新日期：2005-01-31 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(89)90153-3
更新日期：1989-11-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90017-3
更新日期：1985-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.04.013
更新日期：2006-08-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.11.002
更新日期：2010-01-18 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.07.009
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND:Notch pathway is highly conserved across species and is involved in the regulation of cell differentiation and activity both in embryonic development and adult life. Notch signaling has an important role in the development of hematopoietic stem cells and their differentiation to committed lineages, as well a...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2020.04.010
更新日期：2020-07-01 00:00:00
abstract：:A novel mutant strain of rats, LEC, exhibits an arrest of T-cell maturation from CD4+CD8+ to CD4+CD8- but not the CD4-CD8+ cells in the thymus. Nevertheless, CD4+T cells arise in lymph nodes of LEC rats, implying extrathymic T-cell maturation. We analyzed the variable (V) and junctional region diversity of T-cell rece...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)00254-o
更新日期：1995-03-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00209-2
更新日期：2003-01-02 00:00:00
abstract：:Abnormal activation of the transcriptional factor STAT3 (signal transducer and activator of transcription 3) was recently associated with Alzheimer Disease (AD). STAT3 phosphorylation is critical for cytokine secretion linked to neuroinflammation. Moreover, STAT3 may act as a transcriptional regulator of BACE1 (β-APP ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2020.10.004
更新日期：2020-12-01 00:00:00
abstract：:Flt3 ligand, a recently described growth factor affecting early hematopoietic progenitor cells, can also support the expansion of dendritic cells secreting IL-12. Its potential use in a clinical setting has been suggested. Here, we studied the effect of in situ delivery of Flt3 ligand plasmid (FL) on the antibody resp...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00039-1
更新日期：2002-05-01 00:00:00
abstract：:Properly balanced cellular responses require both the mutual interactions of soluble factors with cell surface receptors and the crosstalk of intracellular molecules. In particular, immune cells exposed unceasingly to an array of positive and negative stimuli must distinguish between what has to be tolerated and attac...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2017.08.011
更新日期：2017-10-01 00:00:00
abstract：:ITIM-bearing receptors, a family which only recently has been recognized, play a key role in the regulation of the ITAM-induced activation of immune competent cells. The mechanism of ITM-mediated regulation in various cells was recently clarified. The present review focuses on ITIM bearing membrane proteins that negat...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/s0165-2478(99)00024-3
更新日期：1999-05-03 00:00:00
abstract：:Numerous studies have dealt with occurrence of dendritic cells in various nonlymphoid organs such as kidney, liver or heart, whereas lymphocyte patterns in these organs have not been analyzed in detail. In the present study, leukocytes were quantified as cells/mm2 in the perivascular, interstitial and parenchymal tiss...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(96)02595-3
更新日期：1996-11-01 00:00:00
