Reduction of antibody response against botulinum neurotoxin A by synthetic monomethoxypolyethylene glycol-peptide conjugates.


:Recently, we determined the molecular locations on BoNT/A of the antigenic regions recognized by blocking Abs of cervical dystonia patients immunoresistant to BoNT/A treatment. In the present work we tested the possibility of reducing the levels of the Ab response against immunodominant antigenic sites on the heavy chain of BoNT/A in order to diminish immunoresistance caused by blocking Abs. Four antigenic regions on BoNT/A represented by peptides N8 (residues 547-565), N25 (785-803), C15 (1051-1069) and C31 (1275-1296) were tested for suppressing Ab responses against the correlate regions. The conjugates were synthesized with monomethoxypolyethylene glycol (mPEG) attached to the peptide N-termini. Tolerization with a given mPEG-peptide reduced the Ab levels against the correlate region and the antisera became less protective than antisera of untolerized controls that were immunized only with inactive BoNT/A. On days 31 and 52 in the immunization course mPEG-N8 was most effective and the antisera of tolerized mice were weaker and less protective relative to controls. Other mPEG-peptides were also suppressed the Ab responses to various extents. Bleeds up to 5 months showed that tolerization can be made to persist for the entire period. The results indicated that the tolerization procedure might be potentially useful for clinical applications to immunoresistant patients.


Immunol Lett


Immunology letters


Dolimbek BZ,Aoki KR,Atassi MZ




Has Abstract


2011-06-30 00:00:00














  • Production of human allotype-specific anti-CD45 monoclonal antibodies.

    abstract::The aim of this work was to raise allotype specific monoclonal antibodies to human CD45, with the long-term objective of producing a reagent which could be used to prolong graft survival in renal transplantation through removal of passenger leukocytes from the graft. At present there are no anti-CD45 monoclonal antibo...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Dean GS,Aspinall R

    更新日期:1999-06-01 00:00:00

  • Tumor cells loaded with α-galactosylceramide promote therapeutic NKT-dependent anti-tumor immunity in multiple myeloma.

    abstract::Tumor cells have been used as the tumor antigen sources for developing cancer vaccines. Due to their low immunogenicity, tumor antigens are combined with various adjuvants to enhance immunogenicity of cancer vaccines. Among them, a natural killer T cell (NKT)-ligand, α-galactosylceramide (αGC) has been reported as a p...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Hong S,Lee H,Jung K,Lee SM,Lee SJ,Jun HJ,Kim Y,Song H,Bogen B,Choi I

    更新日期:2013-11-01 00:00:00

  • The rabbit immune system: cells regulating the proliferative response of purified T cells to concanavalin A and phytohaemagglutinin.

    abstract::T cell specific, B cell specific and Ia specific polyclonal antisera, as well as monoclonal antibodies, were employed to characterize cells involved in regulation of T cells, proliferating upon stimulation with concanavalin A or phytohaemagglutinin. Evidence for Ia+ accessory cells was obtained and the constellation o...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Ponsard DC,Chou CT,Cinader B,Dubiski S

    更新日期:1986-08-01 00:00:00

  • Subpopulations of circulating lymphocytes in adults with coeliac disease.

    abstract::Circulating lymphocytes were enumerated in 25 patients with coeliac disease and in 24 healthy donors by immunofluorescent staining using monoclonal antibodies for T cell surface phenotypic markers T11 (mature), T4 (helper) and T8 (suppressor) or polyvalent antisera for surface immunoglobulins (B cells). Proportions of...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Vĕtvicka V,Tlaskalová-Hogenová H,Fric P,Brochier J

    更新日期:1986-08-01 00:00:00

  • Hybridoma autoantibodies to erythrocytes from NZB mice and the induction of hemolytic anemia.

    abstract::We established two clones of monoclonal hybridoma, from a non-immunized NZB mouse, which produce IgM class hemagglutinating autoantibodies reactive with the exposed murine erythrocyte autoantigens. Absorption studies revealed that one monoclonal antibody exhibits cross-reactivity to chick erythrocytes and mouse liver,...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Ozaki S,Nagasawa R,Sato H,Shirai T

    更新日期:1984-01-01 00:00:00

  • Interleukin-2 receptors in infectious mononucleosis.

    abstract::The majority of peripheral blood mononuclear cells (PBMC) from acute-phase infectious mononucleosis (IM) patients express high levels of T10 (CD38) and HLA-DR surface antigens, which are markers characteristic of activated T cells. However, Tac antigen (p55) expression on these cells was not detectable by flow cytomet...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Schmidt C,Burman K,Moss DJ,Ryan J,Burrows SR,Misko IS

    更新日期:1989-12-01 00:00:00

  • The production of human monoclonal anti-MMTV antibodies by in vitro immunization with anti-idiotypic antibodies.

    abstract::We have previously reported the production of a series of human monoclonal antibodies reacting with mouse mammary tumor viral antigens as well as the human counterpart HuMTV antigen. Against two of these antibodies (B11 and 4.6/6), anti-idiotypic antibodies were generated, which appeared to be the internal image of vi...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Blank M,Smorodinsky IN,Keydar I,Chaitchik S,Shoenfeld Y

    更新日期:1991-04-01 00:00:00

  • Regulation of IL-18 expression by CRH in mouse microglial cells.

    abstract::Corticotropin releasing hormone (CRH) is a major regulator of the stress response. This study examined whether CRH regulates interleukin-18 expression on microglia, BV2. Our data show that CRH enhanced IL-18 expression and significantly induced the secretion of functional IL-18 protein. Furthermore, CRH induced IL-18 ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Yang Y,Hahm E,Kim Y,Kang J,Lee W,Han I,Myung P,Kang H,Park H,Cho D

    更新日期:2005-05-15 00:00:00

  • Attenuated nef DNA vaccine construct induces cellular immune response: role in HIV-1 multiprotein vaccine.

    abstract::HIV-1 positive patients generate Nef-specific CTL response, indicating that Nef is a potent immunogen. However, Nef is also known to down regulate the expression of CD4 and MHC-I molecules, thereby protecting virally infected target cells. We compared the immunogenicity of non-functional nef vaccine constructs to wild...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Majumder B,Gray B,McBurney S,Schaefer TM,Dentchev T,Mahalingam S,Reinhart TA,Ayyavoo V

    更新日期:2003-10-31 00:00:00

  • Sex-dependent differences in the IgE response modulated by phytohemagglutinin.

    abstract::Modulation of the IgE response by PHA treatment in relation to animal sex was analysed. Male and female RF mice were immunized with ovalbumin in aluminum hydroxide gel and PHA was injected 1 or 2 days before immunization. Castrated mice were also immunized and received PHA on day -2. The results show that the IgE resp...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Astorquiza MI,Cisternas C,Leal X

    更新日期:1987-10-01 00:00:00

  • Novel intranasal pertussis vaccine based on bacterium-like particles as a mucosal adjuvant.

    abstract::Pertussis, or whooping cough, has recently reemerged as a major public health threat despite high levels of vaccination. The development of a novel pertussis vaccine, especially an intranasal (i.n.) vaccine is undoubtedly necessary, and mucosal adjuvants have been explored to enhance the immune response. In the presen...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Shi W,Kou Y,Jiang H,Gao F,Kong W,Su W,Xu F,Jiang C

    更新日期:2018-06-01 00:00:00

  • Mitogenic effect of prolactin on chicken lymphocytes in vitro.

    abstract::Lymphocytes obtained from thymus and spleens of 1-6 week old White Leghorn cockerels, untreated or immunized twice with sheep red blood cells (SRBC), were cultured with common T-cell mitogens in serial dilutions and/or different concentrations of bovine prolactin (PRL). [3H]Thymidine incorporation in newly synthesized...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Skwarło-Sońta K

    更新日期:1990-06-01 00:00:00

  • Limitation of nitric oxide production: cells from lymph node and spleen exhibit distinct difference in nitric oxide production.

    abstract::Many types of cells in the immune system have been found to produce nitric oxide (NO), which performs multiplex functions. However, in myelin basic protein peptide 68-86 (MBP 68-86)-induced experimental autoimmune encephalomyelitis (EAE) in Lewis rats, we found that elevated NO production was generated from spleen cel...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Xu L,Yang J,Huang Y,van der Meide PH,Levi M,Wahren B,Link H,Xiao B

    更新日期:2000-03-01 00:00:00

  • Cyclophilin A and an antibody against cyclosporin A resemble each other in their binding sites.

    abstract::The three-dimensional structures of cyclosporin A complexed with cyclophilin A or Fab fragment of a monoclonal antibody were compared. In these two complexes conformations of the fragment D-alanine8-N-methylvaline11 in cyclosporin A were in a good agreement. In addition, cyclophilin A and the Fab fragment had related ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Denesyuk AI,Lundell J,Zav'yalov V,Korpela T

    更新日期:1994-07-01 00:00:00

  • IL-6 antisense oligonucleotides inhibit IgE production in IL-4 and anti-CD40-stimulated human B-lymphocytes.

    abstract::Stimulation of tonsillar B-lymphocytes with CD40 antibodies and IL-4 leads to homotypic adhesion, proliferation, and differentiation into Ig-producing cells. It also leads to the production of IL-6, a pleiotropic cytokine involved in B-cell maturation and differentiation. To assess the importance of IL-6 in the differ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Björck P,Larsson S,Andäng M,Ahrlund-Richter L,Paulie S

    更新日期:1998-03-01 00:00:00

  • Acetylation regulates the MKK4-JNK pathway in T cell receptor signaling.

    abstract::T cell functions are regulated by multiple signaling cascades, including the MKK4-JNK (c-Jun NH2 terminal kinase) pathway. However, the mechanism regulating the MKK4-JNK axis in T cells remains unclear. Herein, we demonstrated that protein acetylation modulates JNK activity induced by T cell receptor (TCR) activation....

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Matsui Y,Kuwabara T,Eguchi T,Nakajima K,Kondo M

    更新日期:2018-02-01 00:00:00

  • High dose of lipopolysaccharide pre-treatment prevents OVA-induced anaphylactic decreases in rectal temperature in the immunized mice.

    abstract::It remains unclear whether lipopolysaccharide (LPS) pre-treatment, which prevents Th2-type responses via Toll-like receptor 4 (TLR4), inhibits anaphylaxis. To determine the dose-dependent effects of LPS pre-treatment on anaphylactic decreases in rectal temperature caused by ovalbumin (OVA) re-exposure in immunized mic...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Shiva D,Matsumoto T,Kremenik MJ,Kato Y,Yano H

    更新日期:2008-06-15 00:00:00

  • CD70 expression on T-cell subpopulations: study of normal individuals and patients with chronic immune activation.

    abstract::CD70, the ligand of CD27, is a member of the TNF family, which includes molecules essential in the regulation of lymphocyte growth and survival. It is absent on resting lymphocytes but can be induced in vitro with activating stimuli. To extend information about its expression by different T-cell subpopulations, and it...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Brugnoni D,Airò P,Marino R,Notarangelo LD,van Lier RA,Cattaneo R

    更新日期:1997-02-01 00:00:00

  • Identification of a novel single chain fragment variable antibody targeting CD24-expressing cancer cells.

    abstract::CD24 is a heavy glycosylated protein that is overexpressed in numerous cancer and cancer stem cells. It is involved in the development, invasion, and metastasis of the cancer cells. CD24 can be considered in targeted cancer therapy as a new target. Here, phage display technology was used for the identification of nove...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Eyvazi S,Kazemi B,Bandehpour M,Dastmalchi S

    更新日期:2017-10-01 00:00:00

  • Mechanism of tumor cell-induced T-cell apoptosis mediated by galectin-1.

    abstract::Galectin-1 (Gal-1) has been implicated in tumor progression partly via the induction of T-cell apoptosis. However the mechanism of Gal-1 induced T-cell death was mostly studied using recombinant, soluble Gal-1 producing controversial results. To explore the true mechanism of Gal-1 and hence tumor cell-induced T-cell d...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Kovács-Sólyom F,Blaskó A,Fajka-Boja R,Katona RL,Végh L,Novák J,Szebeni GJ,Krenács L,Uher F,Tubak V,Kiss R,Monostori E

    更新日期:2010-01-04 00:00:00

  • Intramuscular co-injection of naked DNA encoding HBV core antigen and Flt3 ligand suppresses anti-HBc antibody response.

    abstract::Flt3 ligand, a recently described growth factor affecting early hematopoietic progenitor cells, can also support the expansion of dendritic cells secreting IL-12. Its potential use in a clinical setting has been suggested. Here, we studied the effect of in situ delivery of Flt3 ligand plasmid (FL) on the antibody resp...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Kwon TK,Park JW

    更新日期:2002-05-01 00:00:00

  • Hsp90 inhibitor 17-AAG reduces ErbB2 levels and inhibits proliferation of the trastuzumab resistant breast tumor cell line JIMT-1.

    abstract::ErbB2, a member of the EGF receptor family of tyrosine kinases is overexpressed on many tumor cells of epithelial origin and is the molecular target of trastuzumab (Herceptin), the first humanized antibody used in the therapy of solid tumors. Trastuzumab, which is thought to act, at least in part, by downregulating Er...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Zsebik B,Citri A,Isola J,Yarden Y,Szöllosi J,Vereb G

    更新日期:2006-04-15 00:00:00

  • CD45RA-expressing memory/effector Th cells committed to production of interferon-gamma lack expression of CD31.

    abstract::It has been considered before that human naive and memory/effector CD4+ T-cells cannot be subdivided solely according to the differential expression of CD45 isoforms. By the lack of expression of CD31 we have identified a subset of CD4+ CD45RA+ CD31- cells which show distinct features of antigen-experienced Th1 cells....

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Thiel A,Schmitz J,Miltenyi S,Radbruch A

    更新日期:1997-06-01 00:00:00

  • Influence of Yersinia pseudotuberculosis outer proteins (Yops) on interleukin-12, tumor necrosis factor alpha and nitric oxide production by peritoneal macrophages.

    abstract::An essential key to pathogenicity in Yersinia is the presence of a 70 kb plasmid (pYV) which encodes a type-III secretion system and several virulence outer proteins whose main function is to enable the bacteria to survive in the host. Thus, a specific immune response is needed in which cytokines are engaged. The aim ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Monnazzi LG,Carlos IZ,de Medeiros BM

    更新日期:2004-06-15 00:00:00

  • Modification of macrophage response to lipopolysaccharide by fetuin.

    abstract::The physiological role(s) of fetuin, a protein present in plasma and many tissues of developing animals at levels much higher than in the adult, is unknown. Here we show that fetuin can modify the responses of macrophages to lipopolysaccharide (LPS) stimulation. At concentrations of fetuin in the medium, corresponding...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Dziegielewska KM,Andersen NA,Saunders NR

    更新日期:1998-01-01 00:00:00

  • Fab antibodies capable of blocking T cells by competitive binding have the identical specificity but a higher affinity to the MHC-peptide-complex than the T cell receptor.

    abstract::In transplant rejection, graft versus host or autoimmune diseases T cells are mediating the pathophysiological processes. Compared to unspecific pharmacological immune suppression specific inhibition of those T cells, that are involved in the disease, would be an alternative and attractive approach. T cells are activa...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Neumann F,Sturm C,Hülsmeyer M,Dauth N,Guillaume P,Luescher IF,Pfreundschuh M,Held G

    更新日期:2009-08-15 00:00:00

  • Expression and modulation of common acute lymphoblastic leukaemia antigen by B prolymphocytic leukaemia cells.

    abstract::Leukaemic blood lymphocytes from patients with B prolymphocytic leukaemia express the common acute lymphoblastic leukaemia antigen (CALLA). The in vitro incubation of prolymphocytes with leucocyte conditioned medium produced the acquisition of plasma cell morphology, of intracytoplasmic immunoglobulins and the simulta...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: de Paoli P,Battistin S,Fantin O,Nimis R,Reitano M,Santini G

    更新日期:1985-01-01 00:00:00

  • Participation of superoxide in luminol-dependent chemiluminescence triggered by mineral dust in rabbit alveolar macrophages.

    abstract::Luminol-dependent chemiluminescence (CL) triggered by mineral dust in rabbit alveolar macrophages depended on superoxide production as revealed by inhibition by superoxide dismutase and ferricytochrome c. The contribution of superoxide changed in the course of CL peak between 80-20%. The effects of ferricytochrome c w...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Wilhelm J,Vilím V,Brzák P

    更新日期:1987-08-01 00:00:00

  • Functional analysis of Mel-14+ and Mel-14- early precursor cells in the adult mouse thymus.

    abstract::The earliest T-cell precursor population in the adult mouse thymus (low CD4 precursors) may be divided into 85% of cells expressing surface Mel-14 (LECAM-1, the lymphocyte homing receptor) and 15% of cells which are Mel-14. To date, this is the only surface marker for which we have found this population to be heteroge...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Wu L,D'Amico A

    更新日期:1994-05-01 00:00:00

  • A promising new approach of VEGFR2-based DNA vaccine for tumor immunotherapy.

    abstract:AIM:To develop new approach for tumor immunotherapy with VEGFR2-based DNA vaccine. METHODS:VEGFR2 derived from different species were prepared by RT-PCR and subjected to recombinant constructs. The chimeric gene of VEGFR2 was prepared by integrating different epitopes in different species. By transfecting into cells, ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Yan J,Jia R,Song H,Liu Y,Zhang L,Zhang W,Wang Y,Zhu Y,Yu J

    更新日期:2009-09-22 00:00:00