Abstract：
:Murine peritoneal macrophages were isolated by adherence and their listericidal activity assessed in the presence or absence of selected cytokines. Untreated macrophages were not highly listericidal, showing moderate killing in the first 2 h after infection, and allowed progressive microbial growth thereafter (up to 9 h). Pre-treatment of cells with 10 to 100 U/ml of IFN-gamma allowed macrophages to develop sustained listericidal activity for the 9-h observation period, with a 2-log reduction of Listeria CFU per monolayer. Pulsing of cells with TNF-alpha alone did not result in enhanced microbicidal activity but TNF-alpha potentiated IFN-gamma-induced listericidal activity, resulting in high levels of killing when both cytokines were present. Conversely, macrophages pre-treated with interleukin-3 (IL-3) or colony-stimulating factor-1 (CSF-1) were found to be much more permissive for Listeria growth. Neither IL-3 nor CSF-1 abrogated IFN-gamma-induced listericidal activity. Moreover, neither IL-3 nor CSF-1 had any effect on the ability of macrophages to develop a respiratory burst following Listeria infection, as judged by H2O2 release following in vitro infection. Overall, these results suggest that different cytokines may have opposing effects on intracellular microbial growth, and that the balance of cytokine production in vivo may determine the resistance or susceptibility of the infected host.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Denis M,Gregg EOdoi
10.1016/0165-2478(91)90158-7subject
Has Abstractpub_date
1991-03-01 00:00:00pages
237-42issue
3eissn
0165-2478issn
1879-0542pii
0165-2478(91)90158-7journal_volume
27pub_type
杂志文章abstract：:Immunoselection screening methods were developed for the detection of the free heavy chain determinants of biological fluids. The optimal sensitivity range of the electrosyneresis and electroimmunoassay type methods lies between 400 ng and 2 micrograms/ml and between 3 micrograms and 1 mg/ml for immunoglobulins. The s...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90080-4
更新日期：1984-01-01 00:00:00
abstract：:The expression level of complement regulators in ovarian and corpus uteri tumors was not fully established so far. In current manuscript we performed gene expression analysis by the real-time PCR approach to investigate both membrane bound - CD55 and CD59 and fluid phase - factor H and factor H-like 1 complement regul...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2015.06.017
更新日期：2015-10-01 00:00:00
abstract：:In the H-2-incompatible donor-recipient combination (BALB/c----CBA/H) local administration of cyclosporin allows a 4-fold reduction in a single dose prolonging survival of heart tissue allografts. These results suggest that local administration of cyclosporin may be useful for certain grafts. ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90067-z
更新日期：1990-02-01 00:00:00
abstract：:Age-related changes of different individuals multicentric and polymorphic. We have approached this problem of age-related changes by examining (1) membrane densities of receptors of various ligands in different organs, and (2) changes in different classes of T cells and of antibody response. Three strategies have been...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(87)90147-7
更新日期：1987-12-01 00:00:00
abstract：:Cell-surface 5'-nucleotidase was assayed by incubating intact cells with 5' [3H]AMP in iso-osmotic buffer and measuring [3H]adenosine production. The activity of cell surface 5'-nucleotidase in small resting lymphocytes and in cells of the B cell line BCL1 was 5.7 and 1.1 nmol/min/mg protein respectively at 37 degrees...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(88)90122-8
更新日期：1988-09-01 00:00:00
abstract：BACKGROUND:Dendritic cells (DCs) are professional antigen-presenting cells that are capable of inducing immune responses. DC-based vaccines are normally generated using a standard 5- to 7-day protocol. To shorten the DC-based vaccine production for use in cancer immunotherapy, we have developed a fast DC protocol by co...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.03.010
更新日期：2014-07-01 00:00:00
abstract：:The observation that protective immunity induced by immunization with radiation attenuated Plasmodium berghei and Plasmodium yoelii sporozoites is dependent on CD8+ T lymphocytes in some strains of mice led us to speculate that immunization with sporozoites induces cytotoxic T lymphocytes (CTL) that recognize malaria ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90087-7
更新日期：1990-08-01 00:00:00
abstract：:Type 1 diabetes mellitus (T1DM) is a chronic autoimmune disease characterized by the destruction of insulin-secreting β cells upon autoreactive T cell attack. Oral administration of autoantigens is an attractive approach to treating T1DM, but an effective carrier should be used in order to protect antigens. Lactococcu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.04.008
更新日期：2016-06-01 00:00:00
abstract：:Lymphoid cell infiltration of periodontal tissues undergoing pathological destruction suggests a functional interdependence between connective tissue proteins such as collagen, fibronectin and elastin and mononuclear cells, mainly T lymphocytes. The aim of the study was to assess T lymphocyte proliferation after stimu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(98)00060-1
更新日期：1998-10-01 00:00:00
abstract：:African swine fever (ASF) virus has been reported to infect cells of the monocyte family, probably macrophage-like cells, but there is variation in the apparent susceptibility of these cells. We have demonstrated that the phenotype and activity of porcine monocytic cells varies between different isolations and also up...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(93)90136-p
更新日期：1993-07-01 00:00:00
abstract：:The quasimonoclonal (QM) mouse provides a model to analyze B cell selection because major B cell antigen receptors (BCR) are composed of the knockin V(H)DJ(H) 17.2.25 (V(H)T) encoded H chain and the lambda1 or lambda2 L chain, thereby being specific for (4-hydoxy-3-nitrophenyl)acetyl (NP). We have reported that during...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.12.011
更新日期：2007-03-15 00:00:00
abstract：:Cross-linking of BCR rapidly induces protection of B cells from Fas-mediated apoptosis, which has been assumed one of the important survival mechanisms of B cells during antigen stimulation. In the mouse B cell line A20, which is sensitive to Fas-mediated apoptosis, stimulation of BCR inhibited apoptosis induced via F...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.12.009
更新日期：2007-03-15 00:00:00
abstract：:In murine models, challenge with different viral and parasitic infection is closely associated with the production of type I interferons (IFN-alpha/beta) and NK cell production of interferon-gamma (IFN-gamma). Therefore, we wished to determine if IFN-alpha/beta had a role in the regulation of NK cell production of IFN...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00091-6
更新日期：1997-10-01 00:00:00
abstract：:In order to directly demonstrate the roles of CD8(+) T lymphocytes in non-human primates, in vivo depletion of the CD8(+) T cells by administration of a CD8-specific monoclonal antibody (mAb) is one of the crucial techniques. Recently, the common marmoset (Callithrix jacchus), which is classified as a New World monkey...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.08.005
更新日期：2013-07-01 00:00:00
abstract：:Human natural killer (NK) cell activity was not significantly affected by leukotriene (LT) B4 over a wide concentration range (10(-6)-10(-14) M), whether added directly to the NK cell assay or after preincubation of effector cells for 2 h with the drug before addition of Cr labelled K562 target cells. In addition, LTB...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90003-3
更新日期：1985-01-01 00:00:00
abstract：:Phosphatidylinositol 3-kinase (PI3K) is critical player in cell proliferation and survival. The effects of LY294002 and wortmannin, inhibitors of PI3K, on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipoploysaccharide (LPS)-induced Raw 264.7 cells were investigated. Significan...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.12.007
更新日期：2005-06-15 00:00:00
abstract：:Interleukin-35 (IL-35) is a novel anti-inflammatory cytokine and has been shown to play an important role in maintaining immune homeostasis. However, the effect of IL-35 on human asthma remains unclear. The present study is to investigate the expression and significance of IL-35 in childhood asthma. Forty-one asthmati...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.06.002
更新日期：2014-11-01 00:00:00
abstract：:Immunoglobulin species, recombined from Fab and Fc fragments with anti-red cell specificity were used as cytophilic antibodies in studies of triggering the effector activity of immunoglobulins. Recombinant immunoglobulins containing a single Fab' appeared inactive in binding to cell receptors and triggering antibody d...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90046-4
更新日期：1984-01-01 00:00:00
abstract：:During a pathogenic infection, an inflammatory process is triggered in which several inflammatory mediators, such as cytokines, chemokines, growth factors, complement system components, nitric oxide, and others induce integrity alteration on the endothelial barrier. Chemokines are responsible for regulating leukocyte ...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2019.06.001
更新日期：2019-08-01 00:00:00
abstract：:We have investigated the cellular responses of guinea-pig macrophages to human C4a. C4a induced a biphasic Ca2+ mobilization; a rapid temporary Ca2+ mobilization from intracellular Ca2+ pool followed by a weak Ca2+ influx of extracellular Ca2+. Although the C4a-treated macrophages did not respond again to C4a, that is...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(93)90104-a
更新日期：1993-06-01 00:00:00
abstract：:Serum obtained from mice infected with Trypanosoma cruzi have antibodies able to induce immune lysis in presence of complement. Gel filtration in Sephadex G-200 and affinity chromatography with rabbit anti-mouse IgG and protein A-Sepharose showed that the antibodies responsible for the immune serum lytic activity are ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(83)90040-8
更新日期：1983-01-01 00:00:00
abstract：:Culture of high density resting murine B cells with recombinant IL-4 caused changes in the patterns of phosphoproteins associated with the plasma membranes isolated from such cells. The principal difference was the appearance of an alkali-resistant phosphoprotein of Mr = 75,000-80,000. Culture with LPS also gave rise ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(89)90195-8
更新日期：1989-09-01 00:00:00
abstract：:In the present investigations we aimed to study the effect of Shigella sonnei lipopolysaccharide (LPS) on delayed type hypersensitivity (DTH) to non-bacterial antigen in CBA mice. These experiments showed that intraperitoneal injection of phenol-water extracted LPS and avirulent S. sonnei did not affect the level of D...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(01)00298-x
更新日期：2002-01-01 00:00:00
abstract：PURPOSE:Transforming growth factor β (TGF-β) is one of the major immune and inflammation factors responsible for regulating cell proliferation, differentiation, angiogenesis, and immune responses. Deregulated TGF-β activity, especially its influence in peritoneal cytokine cross-talk, has been implicated in pathologies ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2018.10.011
更新日期：2018-09-01 00:00:00
abstract：:To elucidate the role of macrophage migration inhibitory factor (MIF) in ocular inflammation, we examined the localization of MIF in the normal anterior uveal tract of rats. Immunohistochemistry using an anti-MIF antibody revealed that MIF was present in non-pigment epithelial cells of the ciliary body and the epithel...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02600-4
更新日期：1996-10-01 00:00:00
abstract：:Myelin basic protein (MBP), is a major component of the central nervous system (CNS) myelin. MBP can stimulate T cells that migrate into the CNS, initiating a cascade of events that result in perivascular infiltration and demyelination. EAE is an inflammatory and demyelinating autoimmune disease of the CNS that serves...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00089-9
更新日期：1999-06-01 00:00:00
abstract：:Idiopathic thrombocytopenic purpura (ITP) is an acquired autoimmune disorder. Both impaired platelet production and T cell-mediated effects play a role in ITP thrombocytopenia. A Th1 polarization of the immune response, up-regulation of Th17 cells and decreased number of Treg cells have been demonstrated in ITP patien...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.08.006
更新日期：2013-07-01 00:00:00
abstract：:There has been significant progress in the field of heart transplantation over the last 45 years. Although the role of adaptive immunity in heart allograft rejection has been extensively studied for decades, there is increasing evidence that suggests that the innate immune system also contributes to the development of...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2017.11.014
更新日期：2018-02-01 00:00:00
abstract：:Interleukin-37 (IL-37) has been identified as a novel anti-inflammatory cytokine. The present study aimed to evaluate the expression of IL-37 in serum and in peritoneal fluid to determine its clinical significance in endometriosis. Enzyme-linked immunosorbent assay (ELISA) was performed to examine serum IL-37 levels i...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.03.012
更新日期：2017-05-01 00:00:00
abstract：:CD27, which belongs to the TNF receptor family, is a costimulatory molecule that participates in T-cell activation. Unlike costimulatory molecules such as OX40 and 4-1BB, little is known about the role CD27 plays a role in the development of experimental diseases. We asked whether CD27 and its ligand CD70 participate ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2008.05.004
更新日期：2008-08-15 00:00:00
