Abstract：
:Genetic or DNA-based immunization, including genomic immunization, has shown to be a viable alternative approach to induce protective immunity against a number of pathogens in several disease models. Here we describe a new method, cDNA expression library immunization (cDELI), based on the use of a large number of cDNA clones. This immunization strategy was tested in experimental murine Taenia crassiceps cysticercosis model. A partial cDNA expression library of 2 x 10(4) members was constructed in eukaryotic expression vector pcDNA3 and used to immunize BALB/c female mice subcutaneously (s.c.) and intramuscularly (i.m.). In both cases significant reduction of parasite load (up to 65%) was obtained. We were unable to directly measure T. crassiceps-specific humoral immune response in any of the immunized mice, although the expression of pathogen proteins in vitro in macrophages transfected with cDNA expressing plasmids was demonstrated. Also, in three out of five randomly selected immunized mice detectable levels of interferon-gamma (IFN-gamma) were obtained. cDELI has additional advantages compared with recently developed single gene or genomic immunization approaches because a cDNA population represents only those genes that are being expressed in the pathogen cells and the selection of stage-specific antigens is possible. The use of cDELI could be particularly attractive for the pathogens with complicated life cycles and large genomes.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Manoutcharian K,Terrazas LI,Gevorkian G,Govezensky Tdoi
10.1016/s0165-2478(98)00039-xsubject
Has Abstractpub_date
1998-07-01 00:00:00pages
131-6issue
3eissn
0165-2478issn
1879-0542pii
S016524789800039Xjournal_volume
62pub_type
杂志文章abstract：:It was attempted to monitor the immunological response of monoclonal antibodies directed to human alkaline phosphatase in ancient Egyptian bones from the ptolemeic period. The intactness of the respective epitopes of the bone enzyme was successfully demonstrated in an ELISA. Fortunately, the mummified bone was not con...
journal_title：Immunology letters
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更新日期：1995-12-01 00:00:00
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journal_title：Immunology letters
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更新日期：2021-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.04.011
更新日期：2016-06-01 00:00:00
abstract：:This article has been withdrawn at the request of the editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. ...
journal_title：Immunology letters
pub_type： 撤回出版物
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更新日期：2017-08-26 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(95)00059-e
更新日期：1995-07-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：1993-08-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
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更新日期：2000-05-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(92)90220-i
更新日期：1992-12-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.10.003
更新日期：2010-01-04 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(83)90005-6
更新日期：1983-04-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2005.10.015
更新日期：2006-03-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.05.002
更新日期：2013-05-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(87)90147-7
更新日期：1987-12-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(82)90002-5
更新日期：1982-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2017.08.011
更新日期：2017-10-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(95)00051-6
更新日期：1995-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90184-r
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90011-7
更新日期：1984-01-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2005.09.010
更新日期：2006-03-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.12.009
更新日期：2007-03-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2018.11.005
更新日期：2019-08-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00198-0
更新日期：2002-12-03 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(93)90177-4
更新日期：1993-10-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.11.007
更新日期：2007-02-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2021.01.001
更新日期：2021-01-06 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(95)00034-3
更新日期：1995-05-01 00:00:00
abstract：:Interferon inducible transmembrane protein (IFITM) family genes have been implicated in several cellular processes such as the homotypic cell adhesion functions of IFNs and cellular anti-proliferative activities. We previously showed that the IFITM3 single nucleotide polymorphisms (SNPs) associated with susceptibility...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.09.026
更新日期：2013-11-01 00:00:00
