Abstract:
:While the cytoskeletal protein talin binds to the β-chain of LFA-1, the immune cell adaptor SKAP1 (SKAP-55) binds to the α-chain of the same integrin via RapL. Whereas calpain protease cleavage of talin is important for LFA-1 activation, it has been unclear whether SKAP1 can alter the function of talin or its associated adaptor RIAM in T-cells. In this paper, we report that Skap1-/- T-cells showed a reduction in the translocation of talin and RIAM to the contact interface of T-cells with antigenic beads or dendritic cells (DCs) presenting OVA peptide to OT-1 T-cells. In addition, Skap1-/- T-cells show an altered pattern of talin cleavage, while the expression of a cleavage resistant form of talin (L432G) restored the impaired adhesion of OT1 transgenic Skap1-/- T-cells with DCs. SKAP1 therefore can affect the function of talin in T-cells needed for optimal T-cell/DC conjugation.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Lim D,Lu Y,Rudd CEdoi
10.1016/j.imlet.2016.02.004subject
Has Abstractpub_date
2016-04-01 00:00:00pages
40-6eissn
0165-2478issn
1879-0542pii
S0165-2478(16)30013-Xjournal_volume
172pub_type
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doi:10.1016/0165-2478(95)02478-6
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00126-0
更新日期:1998-01-01 00:00:00
abstract::The link between innate and adaptive host immune response to Mycobacterium tuberculosis (M.tb) is driven by dendritic cells (DC). In this study, we examined the ability of prevalent clinical strains from south India (S7, S10) and laboratory strain H37Rv (Rv) to induce maturation of monocyte derived dendritic cells (Mo...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.01.009
更新日期:2008-05-15 00:00:00
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journal_title:Immunology letters
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更新日期:2014-05-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2010.01.004
更新日期:2010-03-10 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90102-1
更新日期:1986-03-01 00:00:00
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doi:10.1016/0165-2478(91)90128-w
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doi:10.1016/j.imlet.2004.05.005
更新日期:2004-07-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.imlet.2009.02.002
更新日期:2009-03-24 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90090-7
更新日期:1984-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00206-6
更新日期:2000-10-03 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.03.010
更新日期:2014-07-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.05.013
更新日期:2005-11-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2011.03.009
更新日期:2011-08-30 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90103-9
更新日期:1987-02-01 00:00:00