Abstract:
UNLABELLED:Helicobacter pylori infection in humans causes gastritis. The infection elicits a complex immune response in which the activation of mast cells and histamine release is of particular importance. Histamine further promotes the immune response and stimulates gastric acid secretion. The inflammatory effects of H. pylori can be studied in intragastrically infected mice. The aim of this study was to compare the local cytokine responses of histamine-deficient, histidine decarboxylase knock-out (HDC KO) and wild-type (WT) mice following H. pylori infection. METHODS:H. pylori was administered intragastrically to HDC KO and WT mice. The animals were infected three times in a 1-week-period and were sacrificed 8 weeks after the first intervention. The local TNF-alpha, IL-6 and IL-10 cytokine levels in gastric mucosal specimens were determined by ELISA. Gastric mucosa sections were also analysed for histological signs of inflammation. To investigate the antibody response following H. pylori infection, the total anti-H. pylori IgG and the ratio of IgG1/IgG2a isotypes were determined in the serum by ELISA. RESULTS:H. pylori induced considerable cytokine production in the infected groups. The TNF-alpha and IL-6 levels were significantly higher in the WT mice than in the HDC KO mice, whereas the IL-10 levels did not differ between the groups. Anti-H. pylori IgG was detected only in the infected groups and the titre was higher in the WT mice. A higher IgG1/IgG2a ratio was observed in the H. pylori infected HDC KO group. Histological analysis revealed that the grades of inflammation were less severe in the infected HDC KO animals. CONCLUSIONS:The results suggest that H. pylori induces lower TNF-alpha and IL-6 secretion in the gastric mucosa in the HDC KO mice than in the WT animals, while the levels of induction of IL-10 were similar. The imbalance between Th1/Th2 is less pronounced in the HDC KO mice, which might explain the milder inflammation in the gastric mucosa. These results provide further information on the role of histamine in the pathomechanism of H. pylori-induced gastritis.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Klausz G,Buzás E,Scharek P,Tiszlavicz L,Gyulai Z,Fülöp AK,Falus A,Mándi Ydoi
10.1016/j.imlet.2004.05.005subject
Has Abstractpub_date
2004-07-15 00:00:00pages
223-8issue
3eissn
0165-2478issn
1879-0542pii
S0165247804001361journal_volume
94pub_type
杂志文章abstract::Bisphenol A (BPA) is a widely used plasticizer, which came into focus because of its genotoxic and sensitizing potential. Besides its toxic properties, BPA is also well-known for its antioxidant chemical properties. This in vitro study investigated the interference of BPA with interferon-γ (IFN-γ)-induced tryptophan b...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2015.10.006
更新日期:2015-12-01 00:00:00
abstract::To monitor genetically-controlled cytokine-producing ability in mice in vitro, we developed a high-density cell culture system, which is preferable for inducing CD4+ T cell-dependent self-priming responses without any antigenic stimulation. When BALB/c spleen cells were cultured at high density (over 1.0 x 10(7) cells...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00143-1
更新日期:1999-12-01 00:00:00
abstract::Fc gamma receptors type IIb1 (Fc gammaRIIb1) inhibit B-cell activation when co-ligated with B-cell antigen receptors (BCR) by immune complexes. In murine B-cells the inhibition is mediated by the interaction of the phosphorylated immunoreceptor tyrosine-based inhibitory motif (P-ITIM) of Fc gammaRIIb1 with the SH2 dom...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(96)02655-7
更新日期:1996-12-01 00:00:00
abstract::A TNF-like factor was purified from lipopolysaccharide (LPS) induced New Zealand white rabbit serum. The TNF-like factor was purified by DEAE-Sephacel, Sephacryl S-200, Mono-Q, CM-affi gel Blue, Superose 12 H/R preparative columns to the specific activity of 4 x 10(6) U/mg protein. The purified protein was 45 kDa in i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00134-x
更新日期:1998-02-01 00:00:00
abstract::Over the last two decades HIV-1 has spread worldwide and has now surpassed malaria as the leading cause of infectious disease mortality in adults (http://www.who.int/infectious-disease-report/pages/ch1text.html). The clinical course and outcome of HIV-1 infection are highly variable among individuals. Most individuals...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(01)00275-9
更新日期:2001-11-01 00:00:00
abstract::Good Syndrome is a rare clinical entity first described as the conjunction of thymoma and hypogammaglobulinemia, and more recently depicted as a complex disease integrating a medical history of thymoma with humoral immunodeficiency (more accurately stated: hypogammaglobulinemia) with or without cellular immunodeficien...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2020.12.010
更新日期:2021-01-05 00:00:00
abstract::Isolation of recombinant antibodies from antibody libraries is commonly performed by different molecular display formats including phage display and ribosome display or different cell-surface display formats. We describe a new method which allows the selection of Escherichia coli cells producing the required single ch...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.02.005
更新日期:2013-03-01 00:00:00
abstract::The prevalence of asthma and allergic disease has increased in many countries and there has been speculation that immunization promotes allergic sensitization. Bordetella pertussis infection exacerbates allergic asthmatic responses. We investigated whether whole-cell pertussis vaccine (Pw) enhanced or prevented B. per...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.10.011
更新日期:2005-02-15 00:00:00
abstract::The presence of dendritic cells (DCs) in mycobacterium-containing granulomas, as well as in other granuloma-inducing diseases, is beginning to be appreciated. This review will summarize what is known about DCs with regards to the granuloma and discuss the potential roles DCs may be playing during mycobacterial infecti...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2009.12.009
更新日期:2010-05-04 00:00:00
abstract::Interleukin 1 beta (IL1 beta) is an inducible polypeptide with many roles in host defence and homoeostasis. It has also been implicated as a mediator of infectious, inflammatory and autoimmune diseases, and the kinetics of its production are relevant to an understanding of the pathogenesis of these conditions. We repo...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90172-7
更新日期:1991-08-01 00:00:00
abstract::Indoleamine 2,3-dioxygenase (IDO) is an enzyme with an immunosuppressive effect whose function is diverted by tumor cells to counteract immune cell functions, inducing immune escape of tumor cells. The aim of this study was to investigate the clinical significance of IDO in nasopharyngeal carcinoma (NPC). Compared to ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2015.11.012
更新日期:2016-01-01 00:00:00
abstract::Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this translocation predicates the subsequent production of IL-2, the me...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2012.01.001
更新日期:2012-02-29 00:00:00
abstract::Mimotopes derived from peptide phage display libraries may reproduce basic functions of epitopes including their antigenicity. In case of toxins, this property makes phage displayed mimotopes highly specific vaccine components free of the toxicity. To explore the potential of mimotopes for vaccine development, their a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00320-0
更新日期:2002-02-01 00:00:00
abstract::Six murine monoclonal antibodies, AT-1, 2, 3, 4, 5, and 6 were produced against human recombinant tumor necrosis factor (gamma TNF). AT-1, -2, -3, -4, and -6 were IgG1, and AT-5 was IgG2a. AT-1, -2, and -3 neutralized the activity of gamma TNF (2 X 10(3) U/ml) over a range of dilutions between 5 and 500 micrograms/ml ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90003-x
更新日期:1988-04-01 00:00:00
abstract::Thymocyte development requires an integration of extracellular cues to enforce lineage commitment at multiple defined checkpoints in a stage-specific manner. Critical signals from the pre-TCR, Notch, and the receptor for interleukin-7 (IL-7) dictate cellular differentiation from the CD4(-)CD8(-) (double negative) stag...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2007.12.008
更新日期:2008-03-15 00:00:00
abstract::While the pathophysiology of burn injury is well established in young adults, the factors that contribute to pathogenesis and increased death in elderly burn patients are not defined. The purpose of this study is to determine the effects of burn injury on mesenteric lymph node (MLN) T cell responses in young and aged ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(03)00022-1
更新日期:2003-04-03 00:00:00
abstract::Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90193-r
更新日期:1992-03-01 00:00:00
abstract::The aim of this work was to raise allotype specific monoclonal antibodies to human CD45, with the long-term objective of producing a reagent which could be used to prolong graft survival in renal transplantation through removal of passenger leukocytes from the graft. At present there are no anti-CD45 monoclonal antibo...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00056-5
更新日期:1999-06-01 00:00:00
abstract::Interleukin-37 (IL-37) has been identified as a novel anti-inflammatory cytokine. The present study aimed to evaluate the expression of IL-37 in serum and in peritoneal fluid to determine its clinical significance in endometriosis. Enzyme-linked immunosorbent assay (ELISA) was performed to examine serum IL-37 levels i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.03.012
更新日期:2017-05-01 00:00:00
abstract::Monoclonal antibodies (mAb) reacting with native (TA5, TB12) and denatured (T33, T35) plant toxin mistletoe lectin I (MLI) from Viscum album have been obtained. The interaction between mAbs and native toxin with ML isoforms (MLII, MLIII) has been investigated. An immunological cross-reaction has been shown to take pla...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)00181-p
更新日期:1995-01-01 00:00:00
abstract::Modulation of the IgE response by PHA treatment in relation to animal sex was analysed. Male and female RF mice were immunized with ovalbumin in aluminum hydroxide gel and PHA was injected 1 or 2 days before immunization. Castrated mice were also immunized and received PHA on day -2. The results show that the IgE resp...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90056-3
更新日期:1987-10-01 00:00:00
abstract::CD24 is an extensively glycosylated membrane protein that is linked to the membrane via a glycosyl-phosphatidylinositol (GPI)-anchor. In mice, CD24 is expressed by hematopoietic and non-hematopoietic cells. CD24-/- mice do not have gross immunological defects, but detailed analysis revealed strongly reduced responses ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.06.004
更新日期:2014-09-01 00:00:00
abstract::Circulating T follicular helper (cTFH) cells have been demonstrated to be involved in B-cell-mediated alloreactive responses in kidney and liver transplantation; however, whether these cells are involved in acute liver allograft rejection after liver transplantation, and which subsets are involved, remains to be clari...
journal_title:Immunology letters
pub_type: 临床试验,杂志文章
doi:10.1016/j.imlet.2019.03.003
更新日期:2019-09-01 00:00:00
abstract::As the most common non-traumatic disabling disease among adolescents, multiple sclerosis (MS) is a devastating neurological inflammatory disease of the central nervous system. Research has not yet fully elucidated its pathogenesis, but it has shown MS to be a complex, multifactorial disease with many interplaying fact...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2020.02.012
更新日期:2020-06-01 00:00:00
abstract::Type I Diabetes mellitus (DM1) is the effect of T cell dependent autoimmune destruction of insulin producing beta cells in the pancreas islet. T cells are activated in response to islet dominant autoantigens, the result being the development of DM1. Insulin is one of the islet autoantigens responsible for activation o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00309-6
更新日期:2001-01-15 00:00:00
abstract::This paper describes functional and genetic studies on the macrophage resistance gene Lsh/Ity/Bcg first described almost two decades ago. Working in vitro with resident peritoneal, liver (Kupffer cells) and bone marrow derived macrophages from congenic B10 (LshS) and B10.L-LshR mice it has been possible to demonstrate...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/0165-2478(91)90032-6
更新日期:1991-10-01 00:00:00
abstract::Culture of high density resting murine B cells with recombinant IL-4 caused changes in the patterns of phosphoproteins associated with the plasma membranes isolated from such cells. The principal difference was the appearance of an alkali-resistant phosphoprotein of Mr = 75,000-80,000. Culture with LPS also gave rise ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90195-8
更新日期:1989-09-01 00:00:00
abstract::We have examined the effect of different monoclonal antibodies (MAb) to the CD11/CD18 family of surface antigens on the production of superoxide anion by phorbol myristate acetate (PMA)-differentiated U-937 cells upon stimulation with concanavalin A (Con A). The anti-CD11c MAb HC1/1 displayed a significant inhibition,...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90079-5
更新日期:1989-02-01 00:00:00
abstract::In a recent, thought-provoking novel by Elizabeth McCracken (The Giant's House. Avon Books, New York, 1997), two characters discuss love and its impossibilities. One brashly claims to be "immune to love", explaining the concept to his perplexed interlocutor, "...people become immune to love like they become immune to ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00181-3
更新日期:1999-03-01 00:00:00
abstract::Colonization with Streptococcus pneumoniae (S. pneumoniae) is associated with an increased risk for recurrent wheeze and asthma. Killed S. pneumoniae showed some potential as an effective immunomodulatory therapy in a murine model of asthma. Murine studies demonstrated protection against allergic asthma by symbiotic b...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.12.001
更新日期:2015-03-01 00:00:00