当前位置： SCI文献检索 > IMMUNOLOGY LETTERS期刊下所有文献 > Self-priming cell culture system for monitoring genetically-controlled spontaneous cytokine-producing ability in mice.

Self-priming cell culture system for monitoring genetically-controlled spontaneous cytokine-producing ability in mice.

Abstract：

:To monitor genetically-controlled cytokine-producing ability in mice in vitro, we developed a high-density cell culture system, which is preferable for inducing CD4+ T cell-dependent self-priming responses without any antigenic stimulation. When BALB/c spleen cells were cultured at high density (over 1.0 x 10(7) cells/well) in 12-well culture plate, they spontaneously produced cytokines including IFN-gamma, IL-2, IL-3, IL-5 and IL-6. The spontaneous cytokine production in this self-priming cell culture (SPCC) system was totally dependent on MHC class II-restricted CD4+ T cells. It was demonstrated that Th2-type BALB/c background mice exhibited higher levels of spontaneous cytokine production in SPCC culture compared with Th1-type C57BL/6 mice. Moreover, using BALB/c x C57BL/6 F1 mice and B10D2 congenic mice, it was demonstrated that highly spontaneous cytokine-producing ability in BALB/c background is genetically dominant and it is controlled by non-MHC genes. Unexpectedly, BALB/c mice spontaneously produced higher levels of IL-2 and IFN-gamma than C57BL/6 mice. However, BALB/c mice revealed lower levels of CTL and NK cell-generation in SPCC system compared with C57BL/6 mice. These results suggested that genetically-controlled predisposition of BALB/c mice toward Th2 immunity appeared not to be derived from their poor IFN-gamma-producing ability but rather derived from their poor responsiveness to IFN-gamma.

journal_name

Immunol Lett

journal_title

Immunology letters

authors

Sato M,Iwakabe K,Ohta A,Sekimoto M,Kimura S,Nishimura T

doi

10.1016/s0165-2478(99)00143-1

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

173-8

issue

3

eissn

0165-2478

issn

1879-0542

pii

S0165247899001431

journal_volume

70

pub_type

杂志文章

相关文献

IMMUNOLOGY LETTERS文献大全
  • Shared recognition molecules in the brain and lymphoid tissues: the polypeptide mediator network of psychoneuroimmunology.

    abstract：:Nervous and immune systems share specific recognition molecules for signals that originate in both systems. The information substances are polypeptides and their receptors. They comprise the multi-directional information exchange network whereby brain and nervous system function including mood and emotion can be integ...

    journal_title：Immunology letters

    pub_type： 杂志文章,评审

    doi：10.1016/0165-2478(87)90171-4

    authors： Wiedermann CJ

    更新日期：1987-12-01 00:00:00

  • Dominance and persistence of donor marrow in long-lived allogeneic radiation chimeras obtained with unmanipulated bone marrow.

    abstract：:Allogeneic, H-2-incompatible irradiation chimeras (H-2d leads to H-2b) constructed with normal, unmanipulated bone marrow and with marrow-derived factors live long and do not manifest a GvH disease. Their response to primary immunization is deficient but their alloreactivity is normal. This chimeric allotolerance cann...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(83)90003-2

    authors： Pierpaoli W,Maestroni GJ

    更新日期：1983-04-01 00:00:00

  • Combinatmarion treatment with Lactobacillus acidophilus LA-1, vitamin B, and curcumin ameliorates the progression of osteoarthritis by inhibiting the pro-inflammatory mediators.

    abstract：:Disease-modifying osteoarthritis (OA) therapy is not yet available. Several adjuvant therapies have demonstrated promising results in the treatment of OA. The present study aimed to investigate the therapeutic effects and underlying mechanisms of a combination of Lactobacillus acidophilus, vitamin B, and curcumin in t...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2020.10.008

    authors： Jhun J,Min HK,Na HS,Kwon JY,Ryu J,Cho KH,Choi J,Jung K,Lee SY,Kim SJ,Yang CW,Park SH,Cho ML

    更新日期：2020-12-01 00:00:00

  • A reduced population of CD103(+)CD11b(+) dendritic cells has a limited impact on oral Salmonella infection.

    abstract：:CD103(+)CD11b(+) dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103(+)CD11b(+) DC...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2016.05.012

    authors： Fernández-Santoscoy M,Wenzel UA,Persson E,Yrlid U,Agace W,Wick MJ

    更新日期：2016-08-01 00:00:00

  • Models and methods for analysis of lymphocyte repertoire generation, development, selection and evolution.

    abstract：:T and B cell receptor repertoires are diversified by variable region gene rearrangement and selected based on functionality and lack of self-reactivity. Repertoires can also be defined based on phenotype and function rather than receptor specificity - such as the diversity of T helper cell subsets. Natural killer (NK)...

    journal_title：Immunology letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.imlet.2012.08.002

    authors： Mehr R,Sternberg-Simon M,Michaeli M,Pickman Y

    更新日期：2012-11-01 00:00:00

  • Fab antibodies capable of blocking T cells by competitive binding have the identical specificity but a higher affinity to the MHC-peptide-complex than the T cell receptor.

    abstract：:In transplant rejection, graft versus host or autoimmune diseases T cells are mediating the pathophysiological processes. Compared to unspecific pharmacological immune suppression specific inhibition of those T cells, that are involved in the disease, would be an alternative and attractive approach. T cells are activa...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2009.06.002

    authors： Neumann F,Sturm C,Hülsmeyer M,Dauth N,Guillaume P,Luescher IF,Pfreundschuh M,Held G

    更新日期：2009-08-15 00:00:00

  • Autoreactivity in HIV-infected individuals does not increase during vaccination with envelope rgp160.

    abstract：:The HIV-1 envelope protein contains several regions with amino acid homology to HLA class I and class II molecules. We evaluated possible changes in antibody responses to those regions during vaccination with rgp 160 produced in a baculovirus system. Forty asymptomatic HIV-infected patients with CD4 cell counts above ...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(94)90125-2

    authors： Lundholm P,Wahren M,Sandström E,Volvovitz F,Wahren B

    更新日期：1994-07-01 00:00:00

  • Cells carrying C5b-9 complement complexes in human atherosclerotic wall.

    abstract：:Fibrous plaques and intimal thickenings of 5 femoral and 5 iliac human arteries obtained at surgery were processed for indirect and double-labeling immunoelectron microscopy using an affinity purified rabbit IgG anti-C5b-9 neoantigen and the EBM 11 monoclonal antibody anti-human macrophages. The C5b-9 complexes were l...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(89)90039-4

    authors： Rus HG,Niculescu F,Poruţiu D,Ghiurca V,Vlaicu R

    更新日期：1989-03-01 00:00:00

  • Enhanced myocardial lesions in chronically Trypanosoma cruzi-infected rats subjected to adult thymectomy.

    abstract：:Control animals and rats infected 90 days earlier, by inoculation of 1 x 10(6) trypomastigotes of Trypanosoma cruzi at weaning, were subjected to adult thymectomy (ATx) or sham operation (S-ATx) and assessed 3 months later for the presence of myocardial lesions and levels of lymph node and spleen T-cell populations. C...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(93)90028-z

    authors： Bottasso OA,Revelli SS,Dávila H,Valenti JL,Musso OC,Ferro ME,Romero-Piffiguer M,Morini JC

    更新日期：1993-08-01 00:00:00

  • Mechanisms of graft failure after human marrow transplantation: a review.

    abstract：:Graft failure (GF) following bone marrow transplantation (BMT) in man is usually attributed to allograft rejection; however, other mechanisms of GF exist. Exposure of the developing allograft to viruses, myelotoxic drugs, and damage to the host-derived marrow stroma during graft-versus-host disease (GVHD) may all caus...

    journal_title：Immunology letters

    pub_type： 杂志文章,评审

    doi：10.1016/0165-2478(91)90204-n

    authors： Hows JM

    更新日期：1991-07-01 00:00:00

  • Acetylation regulates the MKK4-JNK pathway in T cell receptor signaling.

    abstract：:T cell functions are regulated by multiple signaling cascades, including the MKK4-JNK (c-Jun NH2 terminal kinase) pathway. However, the mechanism regulating the MKK4-JNK axis in T cells remains unclear. Herein, we demonstrated that protein acetylation modulates JNK activity induced by T cell receptor (TCR) activation....

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2017.12.002

    authors： Matsui Y,Kuwabara T,Eguchi T,Nakajima K,Kondo M

    更新日期：2018-02-01 00:00:00

  • Superior efficacy of dendritic cell-tumor fusion vaccine compared with tumor lysate-pulsed dendritic cell vaccine in colon cancer.

    abstract：:Dendritic cell (DC)-based tumor vaccine is a promising therapy for malignancies. Recent studies showed greater potency with DC/tumor fusion vaccines against acute myeloid leukemia and melanoma compared with lysate-pulsed DC vaccines. We compared these two vaccine strategies against murine colon cancer and investigated...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2005.05.006

    authors： Kao JY,Zhang M,Chen CM,Chen JJ

    更新日期：2005-11-15 00:00:00

  • Correlation of severity of chronic obstructive pulmonary disease with potential biomarkers.

    abstract：BACKGROUND:Chronic obstructive pulmonary disease (COPD) is a non-specific inflammation, which involves the airways, lung parenchyma and pulmonary vessels. The inflammation causes the activation of inflammatory cells and the release of various inflammatory mediators such as interleukin-1 beta, interleukin-6 and tumor ne...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2018.01.004

    authors： Singh S,Verma SK,Kumar S,Ahmad MK,Nischal A,Singh SK,Dixit RK

    更新日期：2018-04-01 00:00:00

  • In vitro sensitization using thymocyte conditioned medium prepared from fetal calf, horse and rabbit sera.

    abstract：:Thymocyte conditioned medium (TCM) was prepared using 2 lots of fetal calf, and 1 lot each of horse and rabbit sera. The 4 TCM and the respective sera alone were compared in their ability to support primary in vitro sensitization to sheep red blood cells (SRBC) as assayed by direct plaque formation. As would be expect...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(87)90084-8

    authors： White LL,Smith RA

    更新日期：1987-01-01 00:00:00

  • Dispensable role for 4-1BB and 4-1BBL in development of vaccinia virus-specific CD8 T cells.

    abstract：:CD8 T cells are strongly induced in response to certain strains of vaccinia virus (VACV) and the generation of this population is tightly regulated by two Tumor Necrosis Factor (TNF)/TNFR superfamily members, OX40 (CD134) and CD27. In this study, we examined the role of another member of the TNFR superfamily, 4-1BB (C...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2011.10.008

    authors： Zhao Y,Croft M

    更新日期：2012-01-30 00:00:00

  • Protein energy malnutrition alters mucosal IgA responses and reduces mucosal vaccine efficacy in mice.

    abstract：:Oral vaccine responsiveness is often lower in children from less developed countries. Childhood malnutrition may be associated with poor immune response to oral vaccines. The present study was designed to investigate whether protein energy malnutrition (PEM) impairs B cell immunity and ultimately reduces oral vaccine ...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2017.08.025

    authors： Rho S,Kim H,Shim SH,Lee SY,Kim MJ,Yang BG,Jang MH,Han BW,Song MK,Czerkinsky C,Kim JO

    更新日期：2017-10-01 00:00:00

  • Speculations on the origin of the vertebrate immune system.

    abstract：:As one studies more and more the immune systems of invertebrates their multiplicity and diversity increase. On one hand, the number of different local and systemic innate mechanisms (or cascades of mechanisms) increases with every phylum studied, on the other hand the diversity increases within each system as multiple...

    journal_title：Immunology letters

    pub_type： 杂志文章,评审

    doi：10.1016/j.imlet.2003.10.012

    authors： Du Pasquier L

    更新日期：2004-03-29 00:00:00

  • A specific type of membrane microdomains is involved in the maintenance and translocation of kinase active Lck to lipid rafts.

    abstract：:Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this translocation predicates the subsequent production of IL-2, the me...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2012.01.001

    authors： Ballek O,Broučková A,Manning J,Filipp D

    更新日期：2012-02-29 00:00:00

  • Frequencies of epitope-specific cytotoxic T lymphocytes in active chronic viral hepatitis B infection by using MHC class I peptide tetramers.

    abstract：:Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) are thought to play key roles in viral control and liver damage. We have used HLA-A*02 tetramer complex to human HBV core 18-27 (Tc 18-27), envelope 183-191 (Te 183-191), envelope 335-343 (Te 335-343), and polymerase 575-583 (Tp 575-583) epitopes to chara...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2004.01.002

    authors： Wu Y,Zhang J,Chen S,Chen A,Wang L,Li J,Zhao T,Zou L,Tang Y,Tingrong L,Wang F

    更新日期：2004-04-15 00:00:00

  • Administration of MIP-3alpha gene to the tumor following radiation therapy boosts anti-tumor immunity in a murine model of lung carcinoma.

    abstract：:Dendritic cells (DC), the most potent antigen presenting cells (APC), have been shown able to process apoptotic tumor cells and necrotic tumor cells for antigen presentation. Apoptosis and necrosis are the two common final pathways through which the tumors are killed by chemotherapy or radiation therapy. The tumor cel...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2005.09.010

    authors： Zhu B,Zou L,Cheng X,Lin Z,Duan Y,Wu Y,Zhou F,Chen Z

    更新日期：2006-03-15 00:00:00

  • Induction and characterization of antisera against terminal and internal peptides of SV40 large T antigen.

    abstract：:Thirteen synthetic peptides corresponding to different regions of the SV40 large T antigen were used as immunogens after coupling to a carrier protein. All peptide conjugates elicited sera that recognized the inducing peptide. In 10 cases the corresponding sites in the native large T antigen also were recognized, as d...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(86)90006-4

    authors： Friedrich U,Scheidtmann KH,Walter G

    更新日期：1986-04-01 00:00:00

  • Seroma fluid subsequent to axillary lymph node dissection for breast cancer derives from an accumulation of afferent lymph.

    abstract：:Seroma is a frequent complication of breast cancer surgery, the etiology of which remains indefinite. It represents a subcutaneous accumulation of fluid frequently reported after surgical procedures such as axillary lymph node dissection. Despite previous studies have associated seroma fluid to an inflammatory exudate...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2010.03.002

    authors： Montalto E,Mangraviti S,Costa G,Carrega P,Morandi B,Pezzino G,Bonaccorsi I,Cancellieri A,Mingari MC,Mesiti M,Ferlazzo G,Melioli G

    更新日期：2010-06-15 00:00:00

  • The anti-inflammatory response dominates after septic shock: association of low monocyte HLA-DR expression and high interleukin-10 concentration.

    abstract：:The diminished expression of HLA-DR on monocytes has been proposed as a reliable marker of immunosuppression occuring during septic shock. The objective of the present observational study was to establish the time-dependent relation between plasma cytokines interleukin (IL)-10, transforming growth factor (TGF)-beta1, ...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2004.07.009

    authors： Monneret G,Finck ME,Venet F,Debard AL,Bohé J,Bienvenu J,Lepape A

    更新日期：2004-09-01 00:00:00

  • PON1 gene polymorphisms and plasma PON1 activities in Takayasu's arteritis disease.

    abstract：BACKGROUND:Takayasu's arteritis (TA) is a chronic inflammation that affects the large vessels; however, its etiology is still unknown. Human serum paraoxonase hydrolyzes oxidized lipids into low-density lipoproteins and could therefore be associated with the prevalence of inflammation processes. Therefore, the purpose ...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2013.04.005

    authors： Huesca-Gómez C,Soto ME,Castrejón-Téllez V,Pérez-Méndez O,Gamboa R

    更新日期：2013-04-01 00:00:00

  • Normal and tumor-bearing host macrophage responses: variability in accessory function, surface markers, and cell-cycle kinetics.

    abstract：:Normal and tumor-bearing host (TBH) peritoneal macrophage (M phi) responses to in vitro lipopolysaccharide (LPS) treatment were measured by assessing functional and phenotypic changes. Both normal and TBH untreated M phi suppressed mixed lymphocyte reaction (MLR) reactivity at all concentrations. Normal host M phi tre...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(90)90031-k

    authors： Askew D,Yurochko AD,Burger CJ,Elgert KD

    更新日期：1990-03-01 00:00:00

  • Kinetics of IL1 beta mRNA and protein accumulation in human mononuclear cells.

    abstract：:Interleukin 1 beta (IL1 beta) is an inducible polypeptide with many roles in host defence and homoeostasis. It has also been implicated as a mediator of infectious, inflammatory and autoimmune diseases, and the kinetics of its production are relevant to an understanding of the pathogenesis of these conditions. We repo...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(91)90172-7

    authors： di Giovine FS,Symons JA,Duff GW

    更新日期：1991-08-01 00:00:00

  • Mechanism of tumor cell-induced T-cell apoptosis mediated by galectin-1.

    abstract：:Galectin-1 (Gal-1) has been implicated in tumor progression partly via the induction of T-cell apoptosis. However the mechanism of Gal-1 induced T-cell death was mostly studied using recombinant, soluble Gal-1 producing controversial results. To explore the true mechanism of Gal-1 and hence tumor cell-induced T-cell d...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2009.10.003

    authors： Kovács-Sólyom F,Blaskó A,Fajka-Boja R,Katona RL,Végh L,Novák J,Szebeni GJ,Krenács L,Uher F,Tubak V,Kiss R,Monostori E

    更新日期：2010-01-04 00:00:00

  • TIM-1 regulates macrophage cytokine production and B7 family member expression.

    abstract：:T-cell immunoglobulin mucin-1 (TIM-1) is associated with the regulation of T helper type 2 (Th2) immune responses and has been associated with asthma susceptibility. Previous studies have shown that administration of TIM-1 results in T cell hyperproliferation and increased Th2 cytokine secretion. TIM-1 has also been s...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2006.11.004

    authors： Hein RM,Woods ML

    更新日期：2007-01-15 00:00:00

  • Detection and immunolocalization of macrophage migration inhibitory factor in rat iris and ciliary epithelium.

    abstract：:To elucidate the role of macrophage migration inhibitory factor (MIF) in ocular inflammation, we examined the localization of MIF in the normal anterior uveal tract of rats. Immunohistochemistry using an anti-MIF antibody revealed that MIF was present in non-pigment epithelial cells of the ciliary body and the epithel...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/0165-2478(96)02600-4

    authors： Matsuda A,Kotake S,Tagawa Y,Matsuda H,Nishihira J

    更新日期：1996-10-01 00:00:00

  • Lymphocyte subsets are influenced by positivity levels in healthy subjects before and after mild acute stress.

    abstract：:In the current study, the possible association of positivity (POS), recently defined as general disposition to view life under positive outlook, with immune markers and post-stress modifications, was analyzed. Circulating lymphocyte subsets and serum cytokine levels were evaluated before and after a standard mild acut...

    journal_title：Immunology letters

    pub_type： 杂志文章

    doi：10.1016/j.imlet.2017.05.012

    authors： Caprara GV,Nisini R,Castellani V,Vittorio P,Alessandri G,Vincenzo Z,Claudia F,Valentina G,Andrea PD,Biondo MI,Milanetti F,Salerno G,Vincenzo V,Mario P,Aniballi E,Simonetta S,Angela S,D'Amelio R

    更新日期：2017-08-01 00:00:00