Abstract:
:Normal and tumor-bearing host (TBH) peritoneal macrophage (M phi) responses to in vitro lipopolysaccharide (LPS) treatment were measured by assessing functional and phenotypic changes. Both normal and TBH untreated M phi suppressed mixed lymphocyte reaction (MLR) reactivity at all concentrations. Normal host M phi treated with LPS for 3 h were suppressive at all concentrations. TBH M phi treated with LPS for 3 h were not suppressive in the MLR until more than 5% were added. Surprisingly, 24 h treatment of normal and TBH M phi with LPS induced cells that significantly enhanced MLR reactivity when added at 2% or 5%. These cells were not suppressive until a 20% M phi concentration was reached. LPS treatment of normal and TBH M phi changed the percentage of cells expressing the surface markers Mac-1, -2, -3, and Ia as determined by flow cytometry. Normal host peritoneal M phi treated with LPS for 3 h had decreased Mac-1 and -3 expression, but there was no change in Mac-2 or Ia. Plating for 24 h did not change the percentage of M phi expressing Mac-1, -3, or Ia but did cause an increase in Mac-2+ M phi. Treatment of normal host M phi with LPS for 24 h led to a decrease in Mac-1+ and Ia+ M phi, no change in Mac-3+ M phi, but an increase in Mac-2+ M phi. LPS treatment of TBH M phi for 3 h decreased the number of Mac-1+ M phi, but Mac-2+, -3+, or Ia+ M phi numbers did not change. Plating TBH M phi for 24 h caused a decrease in the number of Mac-1+ M phi, no change in Mac-3+ or Ia+ M phi, but an increase in Mac-2+ M phi. Treatment with LPS for 24 h led to no change in the number of Mac-1+, -3+, or Ia+ TBH M phi, but Mac-2+ M phi increased. The phenotypic and functional changes after LPS treatment led us to ask if these changes were detectable at the level of DNA and RNA. Flow cytometric analysis of acridine orange-stained M phi was used to measure DNA and RNA levels. This analysis determines M phi cell-cycle kinetics and estimates their RNA synthesis. In normal host M phi, a 3-h LPS treatment caused a decrease of cells in G0/G1 but an insignificant change in RNA levels.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Askew D,Yurochko AD,Burger CJ,Elgert KDdoi
10.1016/0165-2478(90)90031-ksubject
Has Abstractpub_date
1990-03-01 00:00:00pages
21-9issue
1eissn
0165-2478issn
1879-0542pii
0165-2478(90)90031-Kjournal_volume
24pub_type
杂志文章abstract::NK-like T cells which express the NK1.1 molecule and CD3 (or TCR) of intermediate level (CD3int or TCRint cells) were recently demonstrated to be present in various immune organs, and to have NK-like cytotoxic activity against NK target cells. In this study, we investigated whether NK1.1- T cells could express NK1.1. ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00014-5
更新日期:1998-04-01 00:00:00
abstract::We studied isotype profiles of anti-HIV antibodies (Ab) in HIV-1-infected African patients with high viral loads and major B cell dysfunction. We focussed on IgG1, IgG3, and IgA as these classes and subclasses tend to support neutralizing functions against HIV. Total IgG1, IgG3 and IgA were detected in the plasma of b...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.02.004
更新日期:2004-04-30 00:00:00
abstract::Serum obtained from mice infected with Trypanosoma cruzi have antibodies able to induce immune lysis in presence of complement. Gel filtration in Sephadex G-200 and affinity chromatography with rabbit anti-mouse IgG and protein A-Sepharose showed that the antibodies responsible for the immune serum lytic activity are ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90040-8
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:Behçet's disease (BD) is a chronic multi-factorial inflammatory disease with the important role of genetic in activation of its inflammatory response. Interleukin (IL)-33 is a member of the IL-1 family of cytokines that affects innate and adaptive immune systems to promote inflammatory responses. In the curre...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2018.11.005
更新日期:2019-08-01 00:00:00
abstract::This paper describes functional and genetic studies on the macrophage resistance gene Lsh/Ity/Bcg first described almost two decades ago. Working in vitro with resident peritoneal, liver (Kupffer cells) and bone marrow derived macrophages from congenic B10 (LshS) and B10.L-LshR mice it has been possible to demonstrate...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/0165-2478(91)90032-6
更新日期:1991-10-01 00:00:00
abstract::A rapid, sensitive and simple method is provided for detecting Bence-Jones proteins in unconcentrated urine by electrosyneresis. This is based on the fact that the free light-chains, similar to the intact immunoglobulin molecules, form complexes with albumin under the effect of glutaraldehyde. Consequently, the origin...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90005-6
更新日期:1983-04-01 00:00:00
abstract::As a cytokine adjuvant, granulocyte-macrophage colony-stimulating factor (GM-CSF) has been demonstrated to play central roles in the enhancement of the immune response and protection elicited by experimental vaccines. However, in our previous work, the co-administration of GM-CSF produced untoward effects on the immun...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.08.005
更新日期:2014-11-01 00:00:00
abstract::The prevalence of asthma and allergic disease has increased in many countries and there has been speculation that immunization promotes allergic sensitization. Bordetella pertussis infection exacerbates allergic asthmatic responses. We investigated whether whole-cell pertussis vaccine (Pw) enhanced or prevented B. per...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.10.011
更新日期:2005-02-15 00:00:00
abstract::Sera from 2 subacute sclerosing panencephalitis (SSPE) patients were absorbed with a concentrated preparation of measles virus. Measles-specific IgG was eluted from the precipitates containing measles antigen-antibody complex. These IgGs, when subjected to immunofixation after isoelectric focusing showed a number of o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90002-5
更新日期:1982-02-01 00:00:00
abstract::In spite of impressive results in the treatment of acute lymphoblastic B cell leukemia (B-ALL) with chimeric antigen receptor (CAR) T cells, the clinical outcome of some hematological cancers like follicular lymphoma (FL) and chronic lymphocytic leukemia (CLL) has not been very promising likely due to immunosuppressiv...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.04.005
更新日期:2020-07-01 00:00:00
abstract::Antibodies reactive with native double stranded DNA are characteristic of the chronic inflammatory disease systemic lupus erythematosus. Native DNA is however, a poor immunogen and the mechanism of anti-DNA antibody production is incompletely understood. Modification of DNA can increase its immunogenicity and in infla...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90237-i
更新日期:1992-10-01 00:00:00
abstract::Administration of opioids that bind to the classical mu opioid receptor has been shown to lead to unintended alterations in immune function. Traditionally, altered immune function has been investigated with circulating immune cells. Effects of mu agonists on intestinal epithelial immune function have not been describe...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00198-0
更新日期:2002-12-03 00:00:00
abstract::Idiopathic thrombocytopenic purpura (ITP) is an acquired autoimmune disorder. Both impaired platelet production and T cell-mediated effects play a role in ITP thrombocytopenia. A Th1 polarization of the immune response, up-regulation of Th17 cells and decreased number of Treg cells have been demonstrated in ITP patien...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.08.006
更新日期:2013-07-01 00:00:00
abstract::The aim of the present work was to develop an in vitro model of eosinophil cytotoxicity which mimics numerous in vivo situations characterized by complement activation through the alternative pathway. Our results demonstrate that eosinophilic granulocytes from patients with parasitic diseases and blood eosinophilia we...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90078-7
更新日期:1986-01-01 00:00:00
abstract::Herein, we summarize the steps of a common scientific path taken by the two Guest Editors, an Anesthesiologist (EA) and an Immunologist (AS), and started 25 years ago at the National Cancer Institute in Rome. When in 1980 WHO codified the usage of opioids for cancer pain relief, it was matter of debate whether only di...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2020.06.011
更新日期:2020-10-01 00:00:00
abstract::Lyophilized bacterial lysates, which actively stimulate the immune response, are widely used as vaccines or 'biological response modifiers' in subjects with recurrent bacterial respiratory infections. Since vaccines are indicated in the absence or in the presence of a weak constitutive immune response activity, a bett...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00290-0
更新日期:2003-03-03 00:00:00
abstract:OBJECTIVES:To investigate the associations of cytochrome p450 1A1 (CYP1A1) and manganese superoxide dismutase (MnSOD) genes polymorphisms with the susceptibility to AS in Taiwan. METHODS:The polymorphisms of CYP1A1 and MnSOD genes were determined in 70 patients with ankylosing spondylitis (AS) and 93 healthy controls ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(03)00071-3
更新日期:2003-08-05 00:00:00
abstract::When IL-4 mRNA was distinguished from mRNA encoding its antagonist, the splice variant IL-4delta2, it was found to correlate directly with expression of TLR2 in fresh peripheral blood mononuclear cells (PBMCs) from normal donors (p=0.0013). Similarly IL-4 mRNA was high when TLR2 mRNA was abundant compared to levels of...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.06.001
更新日期:2006-08-15 00:00:00
abstract::Recent data provide evidence that antigen-specific CD4+ T-cell clones or antigen-activated T-cell lines can kill antigen-presenting cells (APC). We focused our studies on monocytes acting as APC in cultures of T cells freshly isolated from peripheral blood. The presence of monocytes in culture was monitored by their a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00051-6
更新日期:1995-06-01 00:00:00
abstract::The inflammatory response plays an important role during the induction of several neonatal diseases. Previous studies have shown that during newborn infections, the natural imbalance between pro- and anti-inflammatory responses shifts toward the production of pro-inflammatory cytokines. In this study, we employed an a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2015.12.004
更新日期:2016-02-01 00:00:00
abstract::It has been previously shown that normal human peripheral blood mononuclear cells produce a soluble factor(s) that causes selective inhibition of collagen production by cultured human diploid fibroblasts, and that stimulation of the mononuclear cells by appropriate mitogens greatly enhances its production. Because of ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90145-2
更新日期:1985-01-01 00:00:00
abstract::In addition to its hematopoietic activities, interleukin-3 (IL-3) can modulate macrophage functions. We have studied the production of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor (TNF) by mouse peritoneal macrophages triggered by lipopolysaccharide (LPS) in the presence or absence of IL-3. Int...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90109-n
更新日期:1991-05-01 00:00:00
abstract::Autoimmunity in disease is driven by autoantigen. Cell surface molecules may stimulate autoreactive T-helpers if class II MHC is expressed; special factors may predispose to the ease of class II induction. Soluble autoantigens may be focused by primed B cells and processed for presentation to T cells. Autoantigenicity...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90155-6
更新日期:1987-12-01 00:00:00
abstract:INTRODUCTION:Intrahepatic injection of alloantigen prolongs allograft survival and inhibits T-lymphocyte release of both IL-2 and IFN-gamma but not IL-4. This suggests that intrahepatic processing of antigen lead to a predominance of Th2 cell population with inhibition of Th1 cell type. This study examines the effects ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00322-4
更新日期:2002-04-01 00:00:00
abstract::NADPH oxidase 2 is a superoxide-generating enzymatic complex based on the catalytic subunit gp91phox that is also known as Nox2. Initially identified in neutrophils, NADPH oxidase 2 was long considered responsible only for the killing of phagocytized microorganisms. However, advances in knowledge about redox signallin...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2020.02.009
更新日期:2020-05-01 00:00:00
abstract::Type I Diabetes mellitus (DM1) is the effect of T cell dependent autoimmune destruction of insulin producing beta cells in the pancreas islet. T cells are activated in response to islet dominant autoantigens, the result being the development of DM1. Insulin is one of the islet autoantigens responsible for activation o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00309-6
更新日期:2001-01-15 00:00:00
abstract::There is great interest in the antibodies-to-DNA transformation, since this change is characteristic of autoimmune diseases and contributes to its pathology. After immunization and fusions, 14 hybridomas bearing DNA-hydrolysis activity against pUC19 plasmid DNA were obtained. Genes coding for V(H) and V(L) regions of ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2010.01.004
更新日期:2010-03-10 00:00:00
abstract::Blood coagulation and inflammation pathways are linked in many aspects. A number of serum factors involved in coagulation cascades affect directly or indirectly inflammatory responses, whereas proinflammatory cytokines influence blood coagulation pathways. In this work we demonstrated that thrombin is an effective sti...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(99)00015-2
更新日期:1999-04-15 00:00:00
abstract::Between the ages of 2 to approximately 11 days mice respond to a challenge with syngeneic IgE by producing anti-IgE antibodies; by the age of 2 weeks they are unresponsive. Even adult mice, however, produce high titers of anti-IgE antibodies when immunized with a conjugate of syngeneic IgE and a foreign antigen such a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00087-3
更新日期:1995-09-01 00:00:00
abstract::The complement system is a protein cascade capable of neutralizing invading pathogens. One of its activation pathways is the lectin pathway which is dependent on the binding of MBL or the ficolins. The specificity of L-ficolin binding has been investigated previously and it was observed that binding is dependent on ac...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.03.014
更新日期:2008-06-30 00:00:00