Abstract:
:It has been previously shown that normal human peripheral blood mononuclear cells produce a soluble factor(s) that causes selective inhibition of collagen production by cultured human diploid fibroblasts, and that stimulation of the mononuclear cells by appropriate mitogens greatly enhances its production. Because of the difficulties inherent in obtaining sufficient peripheral blood lymphocytes to purify the collagen synthesis inhibitory factor (CSIF) further and to study in detail its mechanisms of action, we have examined CSIF production by several leukemic human T- and B-lymphocyte cell lines. From the 10 lines tested (4 T- and 6 B-lymphocyte cell lines), we found 2 T-lymphocyte cell lines which produced CSIF constitutively. When the effects of various lymphocyte mitogens on CSIF production by these cell lines were examined, it was found that only phorbol myristic acid was stimulatory. Further studies demonstrated that optimal CSIF production by the phorbol myristic acid-stimulated leukemic T-lymphocytes could be achieved in a chemically defined, serum-free medium. The CSIF in the supernatants from such cultures was further purified by ammonium sulfate precipitation and gel filtration chromatography and it was shown that it had similar properties to those of CSIF produced by normal human peripheral blood lymphocytes. These findings indicate that certain leukemic T-lymphocyte cell lines are capable of CSIF production in continuous cultures. These results will permit the large scale production of CSIF and a better understanding of its mechanisms of action.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Jimenez SA,Rosenbloom Jdoi
10.1016/0165-2478(85)90145-2subject
Has Abstractpub_date
1985-01-01 00:00:00pages
69-74issue
2eissn
0165-2478issn
1879-0542pii
0165-2478(85)90145-2journal_volume
11pub_type
杂志文章abstract::The administration of protein antigens into earthworms Lumbricus terrestris and Eisenia foetida induces the formation of antigen-binding protein (ABP) with the maximum response occurring between days 4 and 8. High proteolytic activities observed both in coelomocytes and in coelomic fluids cause rapid antigen degradati...
journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
abstract::Interleukin (IL)-17A is a cytokine that plays an important role in infectious, autoimmune, and inflammatory diseases. In this study, we found that TCRγδ(+)CD4(-)CD8(-) T cells, but not TCRαβ(+)CD4(+) T cells, are the primary producers of IL-17A in the genital tract of female mice in the steady-state condition. High mR...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2012.05.006
更新日期:2012-09-01 00:00:00
abstract::Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90193-r
更新日期:1992-03-01 00:00:00
abstract::Opioid receptors are expressed not only on neuroendocrine cells but also on immunocompetent cells such as lymphocytes, monocytes and macrophages. micro-Opioid receptor agonists were found to exert immunosuppressive effects, whereas delta-opioid receptor agonists have been shown to act as immunostimulants. delta-Opioid...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.03.006
更新日期:2008-06-15 00:00:00
abstract::The first step in the clearance of apoptotic cells is chemotactic migration of macrophages towards the apoptotic cells guided by find-me signals provided by the dying cells. Upon sensing the chemotactic signals, macrophages release ATP. ATP is then degraded to ADP, AMP and adenosine to trigger purinergic receptors con...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.02.002
更新日期:2017-03-01 00:00:00
abstract::The observation that protective immunity induced by immunization with radiation attenuated Plasmodium berghei and Plasmodium yoelii sporozoites is dependent on CD8+ T lymphocytes in some strains of mice led us to speculate that immunization with sporozoites induces cytotoxic T lymphocytes (CTL) that recognize malaria ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90087-7
更新日期:1990-08-01 00:00:00
abstract::Apoptosis markers and the rate of lymphocyte apoptosis were followed in the peripheral blood of HIV infected persons at various stages of disease. Our study suggests that the early increase in memory cells following therapy may also be due to a significant decrease in apoptosis in this subset. The intrinsic resistance...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00167-9
更新日期:1999-03-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00117-5
更新日期:1999-01-01 00:00:00
abstract::Serum antibodies reactive with intermediate filaments of the cytoskeleton (anti-IF antibodies) are often present in infectious mononucleosis, some other viral diseases, and rheumatoid arthritis. The mechanism of their production is not known, but it is possible that the formation of this and other autoantibodies resul...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90135-3
更新日期:1982-10-01 00:00:00
abstract::Interleukin-37 (IL-37) has been identified as a novel anti-inflammatory cytokine. The present study aimed to evaluate the expression of IL-37 in serum and in peritoneal fluid to determine its clinical significance in endometriosis. Enzyme-linked immunosorbent assay (ELISA) was performed to examine serum IL-37 levels i...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.03.012
更新日期:2017-05-01 00:00:00
abstract::Staphylococcus aureus (S. aureus) can often lead to many life-threatening diseases. It has the ability to invade normal endovascular tissue. Acute inflammation and its resolution are important to ensure bacterial clearance and limit tissue injury. Carbon monoxide (CO) has been shown to exert anti-inflammatory effects ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2018.09.010
更新日期:2018-11-01 00:00:00
abstract::The presence of dendritic cells (DCs) in mycobacterium-containing granulomas, as well as in other granuloma-inducing diseases, is beginning to be appreciated. This review will summarize what is known about DCs with regards to the granuloma and discuss the potential roles DCs may be playing during mycobacterial infecti...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2009.12.009
更新日期:2010-05-04 00:00:00
abstract::The purpose of this study is to show that anti-Leu M1 antibody (anti-CD15), which has different staining characteristics in lymphoid and non-lymphoid cells, reacted against the surface antigen of a defined monoclonal B cell line. This antibody recognizes the sugar moiety, lacto-N-fucopentaose (LNF-III), which is linke...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(89)90153-3
更新日期:1989-11-01 00:00:00
abstract::Establishment and maintenance of pregnancy represents a challenge for the maternal immune system since it has to defend against pathogens and tolerate paternal alloantigens expressed in fetal tissues. Regulatory T (Treg) cells, a subset of suppressor CD4(+) T cells, play a dominant role in the maintenance of immunolog...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2014.06.013
更新日期:2014-11-01 00:00:00
abstract::Vogt-Koyanagi-Harada disease is an autoimmune systemic disorder. In Vogt-Koyanagi-Harada disease, inflammatory disorders occur in multiple organs containing melanocytes, including uvea (resulting in acute bilateral panuveitis), skin (resulting in vitiligo and alopecia), central nervous system (resulting in meningitis)...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00243-7
更新日期:2001-10-01 00:00:00
abstract::We have reported that a cell line, KML1, obtained from lymph node cells of a 16-week-old female MRL/Mp-lpr/lpr (MRL/1) mouse produces a soluble factor(s) that promotes formation of anti-single-stranded (ss) DNA antibodies by cultures of spleen cells from old MRL/l mice (greater than 16 weeks old). Detailed examination...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90035-o
更新日期:1990-03-01 00:00:00
abstract::A series of nor-MDP analogues were evaluated for adjuvanticity in rodents using beta hCG-TT conjugate as the antigen. Of these, one compound, N-acetylnor-muramyl-L-N-methylalanyl-D-isoglutamine octylamide (nor-MDP octylamide (N-Me-Ala] was found to be effective. This compound, formulated with beta hCG-TT in water-in-o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90244-5
更新日期:1991-01-01 00:00:00
abstract::It has been shown that engraftment of human peripheral blood lymphocytes (PBL) from Epstein-Barr virus (EBV) seropositive donors in C.B-17/SCID mice is associated with a high incidence of human B cell tumors. More recently, we described a new approach enabling engraftment of human PBL in normal strains of mice or rats...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(96)02497-2
更新日期:1996-03-01 00:00:00
abstract:UNLABELLED:To study the pathogenic importance of the rheumatoid factor (RF) in rheumatoid arthritis (RA) and to identify genes differentially expressed in patients and healthy individuals, total RNA was isolated from peripheral blood mononuclear cells (PBMC) from eight RF-positive and six RF-negative RA patients, and s...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.03.018
更新日期:2004-05-15 00:00:00
abstract::NK-like T cells which express the NK1.1 molecule and CD3 (or TCR) of intermediate level (CD3int or TCRint cells) were recently demonstrated to be present in various immune organs, and to have NK-like cytotoxic activity against NK target cells. In this study, we investigated whether NK1.1- T cells could express NK1.1. ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00014-5
更新日期:1998-04-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90136-p
更新日期:1993-07-01 00:00:00
abstract::Immunoglobulin species, recombined from Fab and Fc fragments with anti-red cell specificity were used as cytophilic antibodies in studies of triggering the effector activity of immunoglobulins. Recombinant immunoglobulins containing a single Fab' appeared inactive in binding to cell receptors and triggering antibody d...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90046-4
更新日期:1984-01-01 00:00:00
abstract::Antibodies reactive with native double stranded DNA are characteristic of the chronic inflammatory disease systemic lupus erythematosus. Native DNA is however, a poor immunogen and the mechanism of anti-DNA antibody production is incompletely understood. Modification of DNA can increase its immunogenicity and in infla...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90237-i
更新日期:1992-10-01 00:00:00
abstract::Rheumatic fever (RF) and chronic rheumatic heart disease (RHD) are complications of oropharyngeal infection caused by Streptococcus pyogenes. Despite the importance of the complement system against infections and autoimmunity diseases, studies on the role of the lectin pathway in RF and RHD are scarce. Thus, our aim w...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.11.006
更新日期:2021-01-01 00:00:00
abstract::Mouse spleen cells were activated with IL2 for 4 days in the presence or absence of paraformaldehyde fixed YAC (PFY) tumor cells (spleen cell: PFY ratio 100:1). Fresh spleen cells had poor expression of Ly49A but the expression was significantly upregulated by IL2 activation. Addition of PFYs resulted in a further boo...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00206-1
更新日期:2001-06-01 00:00:00
abstract::Callithrix jacchus, the common marmoset, is a small new world primate that is considered effective as an experimental animal model for various human diseases. In this study, we generated monoclonal antibodies (mAbs) against common marmoset lymphocytes for immunological studies on the common marmoset. We established fi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.09.007
更新日期:2008-12-22 00:00:00
abstract::CBA spleen cells have been stimulated in vitro with A/Jap influenza virus-infected CBA spleen cells to generate a 'primary' cytotoxic lymphocyte (CL) response. The culture conditions were devised to allow the segregation of individual clones of CL and cytotoxicity measured by the lysis of infected or non-infected L-92...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90127-4
更新日期:1982-01-01 00:00:00
abstract::During a pathogenic infection, an inflammatory process is triggered in which several inflammatory mediators, such as cytokines, chemokines, growth factors, complement system components, nitric oxide, and others induce integrity alteration on the endothelial barrier. Chemokines are responsible for regulating leukocyte ...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2019.06.001
更新日期:2019-08-01 00:00:00
abstract::B cell activating factor belonging to the TNF family (BAFF, also called BLyS, TALL-1, THANK, or zTNF4) is an important survival factor for B cells, and is able to regulate T cell activation. Recently, we have demonstrated that human soluble BAFF (hsBAFF) potently elevates NK cell activity of murine splenic cells in vi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.07.005
更新日期:2008-10-30 00:00:00
abstract::Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) are thought to play key roles in viral control and liver damage. We have used HLA-A*02 tetramer complex to human HBV core 18-27 (Tc 18-27), envelope 183-191 (Te 183-191), envelope 335-343 (Te 335-343), and polymerase 575-583 (Tp 575-583) epitopes to chara...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.01.002
更新日期:2004-04-15 00:00:00