Novel monoclonal antibodies recognizing different subsets of lymphocytes from the common marmoset (Callithrix jacchus).


:Callithrix jacchus, the common marmoset, is a small new world primate that is considered effective as an experimental animal model for various human diseases. In this study, we generated monoclonal antibodies (mAbs) against common marmoset lymphocytes for immunological studies on the common marmoset. We established five hybridoma clones, 6C9, 10D7, 6F10, 7A4 and 5A1, producing anti-marmoset mAbs against cell surface antigens on marmoset T and/or B lymphocytes. We confirmed that 6C9 and 10D7 antibodies recognized CD45 antigen, and 6F10 antibody recognized CD8 antigen by flow cytometry using marmoset cDNA transfectants. We also tested them for application of immunoprecipitation, Western blot analysis and immunohistochemistry. We found that immunohistochemistry using marmoset spleen sections could be applied with all established mAbs but immunoprecipitation and the Western blot analysis could be applied with 6F10 and 10D7 antibodies but not with the other three mAbs. These results show that these monoclonal antibodies are useful for advancing immunological research on the common marmoset.


Immunol Lett


Immunology letters


Ito R,Maekawa S,Kawai K,Suemizu H,Suzuki S,Ishii H,Tanioka Y,Satake M,Yagita H,Habu S,Ito M




Has Abstract


2008-12-22 00:00:00














  • Integration of activatory and inhibitory signals in human B-cells.

    abstract::Fc gamma receptors type IIb1 (Fc gammaRIIb1) inhibit B-cell activation when co-ligated with B-cell antigen receptors (BCR) by immune complexes. In murine B-cells the inhibition is mediated by the interaction of the phosphorylated immunoreceptor tyrosine-based inhibitory motif (P-ITIM) of Fc gammaRIIb1 with the SH2 dom...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Sármay G,Koncz G,Gergely J

    更新日期:1996-12-01 00:00:00

  • Cytokine sensitivity of human NK cells during immunosenescence. 2. IL2-induced interferon gamma secretion.

    abstract::A majority of natural killer (NK) cells constitutively express intermediate affinity IL2 receptors made of beta gamma chains and respond by way of enhanced cytokine secretion. However, alterations in cytokine sensitivity of purified NK cells with respect to cytokine secretion during immunosenescence have not been exam...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Krishnaraj R,Bhooma T

    更新日期:1996-04-01 00:00:00

  • Probing molecular changes induced in DNA by reactive oxygen species with monoclonal antibodies.

    abstract::Antibodies reactive with native double stranded DNA are characteristic of the chronic inflammatory disease systemic lupus erythematosus. Native DNA is however, a poor immunogen and the mechanism of anti-DNA antibody production is incompletely understood. Modification of DNA can increase its immunogenicity and in infla...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Blount S,Griffiths HR,Staines NA,Lunec J

    更新日期:1992-10-01 00:00:00

  • Preferential utilisation of deoxycytidine by undifferentiated (peanut positive) tonsillar lymphocytes.

    abstract::Peanut agglutinin (PNA), a D-galactose specific lectin, agglutinated 10-18% of lymphocytes isolated from tonsils of 3- to 6-yr-old children. PNA+ cells were found to be mainly B lymphocytes showing a 9.6 times higher specific activity of DNA polymerase alpha compared to the PNA- cells. The specific activity of deoxycy...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Taljanidisz J,Spasokukotskaja T,Sasvári-Székely M,Antoni F,Staub M

    更新日期:1987-06-01 00:00:00

  • Expression and modulation of common acute lymphoblastic leukaemia antigen by B prolymphocytic leukaemia cells.

    abstract::Leukaemic blood lymphocytes from patients with B prolymphocytic leukaemia express the common acute lymphoblastic leukaemia antigen (CALLA). The in vitro incubation of prolymphocytes with leucocyte conditioned medium produced the acquisition of plasma cell morphology, of intracytoplasmic immunoglobulins and the simulta...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: de Paoli P,Battistin S,Fantin O,Nimis R,Reitano M,Santini G

    更新日期:1985-01-01 00:00:00

  • Human macrophages respond to LPS in a serum-independent, CD14-dependent manner.

    abstract::Two crucial mediators of monocyte activation by lipopolysaccharide (LPS) are the acute phase plasma factor, lipopolysaccharide binding protein (LBP) and cell-surface-expressed CD14. Whether macrophage (M phi) recognized and respond to LPS in a similar manner is unknown. Here we show that human monocyte-derived M phi r...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Jungi TW,Brcic M,Eperon S

    更新日期:1996-12-01 00:00:00

  • Liver nonparenchymal cells involved in hyporesponsiveness induced by portal vein injection of alloantigen.

    abstract:INTRODUCTION:Intrahepatic injection of alloantigen prolongs allograft survival and inhibits T-lymphocyte release of both IL-2 and IFN-gamma but not IL-4. This suggests that intrahepatic processing of antigen lead to a predominance of Th2 cell population with inhibition of Th1 cell type. This study examines the effects ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Chen Y,McKenna GJ,Ong C,Mui AL,Chung SW

    更新日期:2002-04-01 00:00:00

  • Induction of complement-mediated lysis of HIV-1 by a combination of HIV-specific and HLA allotype-specific antibodies.

    abstract::HLA-specific antibodies generated by allo-immunization are supposed to be involved in the control of HIV infections by both the neutralizing capacity of HLA-specific antibodies (Abs) and HLA-specific Ab-dependent complement-mediated lysis (CML). We further characterized CML of HIV primary isolates induced by HLA-speci...

    journal_title:Immunology letters

    pub_type: 信件


    authors: Hildgartner A,Wilflingseder D,Gassner C,Dierich MP,Stoiber H,Bánki Z

    更新日期:2009-09-22 00:00:00

  • Systems Biology and immune aging.

    abstract::Many alterations of innate and adaptive immunity are common in the aging population, which reflect a deterioration of the immune system, and have lead to the terms "immune aging" or "immunosenescence". Systems Biology aims to the comprehensive knowledge of the structure, dynamics, control and design that define a give...

    journal_title:Immunology letters

    pub_type: 杂志文章,评审


    authors: O'Connor JE,Herrera G,Martínez-Romero A,de Oyanguren FS,Díaz L,Gomes A,Balaguer S,Callaghan RC

    更新日期:2014-11-01 00:00:00

  • Vaccine-elicited immune responses prevent clinical AIDS in SHIV(89.6P)-infected rhesus monkeys.

    abstract::Accumulating evidence has demonstrated the importance of cytotoxic T lymphocytes (CTLs) and helper T lymphocytes in controlling HIV-1 replication. We have elicited immune responses in rhesus monkeys utilizing DNA vaccines augmented by the administration of IL-2/Ig, a fusion protein consisting of interleukin-2 and the ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Barouch DH,Fu TM,Montefiori DC,Lewis MG,Shiver JW,Letvin NL

    更新日期:2001-11-01 00:00:00

  • Coenzyme Q10 suppresses Th17 cells and osteoclast differentiation and ameliorates experimental autoimmune arthritis mice.

    abstract::Coenzyme Q10 (CoQ10) is a lipid-soluble antioxidant synthesized in human body. This enzyme promotes immune system function and can be used as a dietary supplement. Rheumatoid arthritis (RA) is an autoimmune disease leading to chronic joint inflammation. RA results in severe destruction of cartilage and disability. Thi...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Jhun J,Lee SH,Byun JK,Jeong JH,Kim EK,Lee J,Jung YO,Shin D,Park SH,Cho ML

    更新日期:2015-08-01 00:00:00

  • Studies on natural cytotoxicity of human monocytes in viral hepatitis.

    abstract::Studies on natural cytotoxicity of peripheral blood monocytes were conducted in patients with acute viral hepatitis B, patients with chronic aggressive hepatitis etiopathogenically linked to viral hepatitis B, and in asymptomatic carriers of HBs antigen. In the majority of cases of viral hepatitis in the acute stage o...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Kiczka W,Szkaradkiewicz A,Flieger J

    更新日期:1985-01-01 00:00:00

  • Interleukin-18 induces the production of vascular endothelial growth factor (VEGF) in rheumatoid arthritis synovial fibroblasts via AP-1-dependent pathways.

    abstract::Interleukin-18 (IL-18) is a novel pro-inflammatory cytokine which has been implicated to play a pathogenic role in rheumatoid arthritis (RA). Vascular endothelial growth factor (VEGF) plays an important role in angiogenesis in rheumatoid synoviocytes. In present study, we examined the effect of IL-18 on VEGF productio...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Cho ML,Jung YO,Moon YM,Min SY,Yoon CH,Lee SH,Park SH,Cho CS,Jue DM,Kim HY

    更新日期:2006-03-15 00:00:00

  • Deficiency of monoclonal antibody (Leu 7) defined NK cells in newly diagnosed insulin-dependent diabetes mellitus.

    abstract::Peripheral blood from 11 newly diagnosed patients with insulin-dependent diabetes mellitus (IDDM) was studied for the proportion of monoclonal antibody (HNK 1, Leu 7) defined natural killer (NK) cells using a fluorescence-activated cell sorter analyzer. The proportion of Leu 7+ cells in patients with IDDM (7.0 +/- 4.0...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Chandy KG,Charles MA,Buckingham B,Waldeck N,Kershnar A,Gupta S

    更新日期:1984-01-01 00:00:00

  • Optimal expression of a Fab-effector fusion protein in Escherichia coli by removing the cysteine residues responsible for an interchain disulfide bond of a Fab molecule.

    abstract::Development of novel bi-functional or even tri-functional Fab-effector fusion proteins would have a great potential in the biomedical sciences. However, the expression of Fab-effector fusion proteins in Escherichia coli is problematic especially when a eukaryotic effector moiety is genetically linked to a Fab due to t...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Kang HJ,Kim HJ,Jung MS,Han JK,Cha SH

    更新日期:2017-04-01 00:00:00

  • Rapid up-regulation of c-FLIP expression by BCR signaling through the PI3K/Akt pathway inhibits simultaneously induced Fas-mediated apoptosis in murine B lymphocytes.

    abstract::Cross-linking of BCR rapidly induces protection of B cells from Fas-mediated apoptosis, which has been assumed one of the important survival mechanisms of B cells during antigen stimulation. In the mouse B cell line A20, which is sensitive to Fas-mediated apoptosis, stimulation of BCR inhibited apoptosis induced via F...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Moriyama H,Yonehara S

    更新日期:2007-03-15 00:00:00

  • Chemical induced inflammation of the liver breaks tolerance and results in autoimmune hepatitis in Balb/c mice.

    abstract::Autoimmune hepatitis (AIH) is a chronic liver disease mediated by immunity, and could lead to liver fibrosis and hepatocellular carcinoma. However, the mechanisms for breaking hepatic tolerance and driving AIH still remain elusive. We herein reported that the non-specific liver inflammation triggered by carbon tetrach...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Chi G,Pei JH,Ma QY,Ru YX,Feng ZH

    更新日期:2020-02-01 00:00:00

  • Experimentally induced cutaneous leishmaniasis: are L3T4+ T cells that promote parasite growth distinct from those mediating resistance?

    abstract::A considerable body of evidence from various laboratories indicates that specific T cell responses generated during infection with Leishmania parasites play an important role both in the resolution and progression of cutaneous leishmaniasis. Recent data, summarized in this article, indicate that resolution of lesions ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Müller I,Pedrazzini T,Louis JA

    更新日期:1988-11-01 00:00:00

  • The effect of T cell independent and cross-reactive antigen on the immune response to pneumococcal conjugate vaccination.

    abstract::The effect of priming with various antigens on subsequent vaccination with the pneumococcal conjugate vaccine (CPV) was determined using BALB/c mice. Priming with pneumococcal polysaccharide or cross-reactive polysaccharide did not inhibit the IgG response to CPV immunization. Additionally, live intranasal colonizatio...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Rabquer B,Smithson SL,Shriner A,Julie Westerink MA

    更新日期:2006-08-15 00:00:00

  • Salmonella SPI-2 type III secretion system-dependent inhibition of antigen presentation and T cell function.

    abstract::Salmonella enterica serovars infect a broad range of mammalian hosts, including humans, causing both gastrointestinal and systemic diseases. Effective immune responses to Salmonella infections depend largely on CD4+ T cell activation by dendritic cells (DCs). Bacteria are internalised by intestinal DCs and respond by ...

    journal_title:Immunology letters

    pub_type: 杂志文章,评审


    authors: Cerny O,Holden DW

    更新日期:2019-11-01 00:00:00

  • The effect of exercise on basophil histamine release in patients with bronchial asthma.

    abstract::There is increasing evidence for the role of basophils in allergic bronchial asthma. We studied the potential role of basophils in the pathogenesis of post-exercise-induced bronchoconstriction by measuring the histamine release from basophils both spontaneously and following ConA, FMLP, anti-IgE and TPA treatment. Two...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Kivity S,Schwarz I,Agami O,Topilsky M,Fireman E

    更新日期:1994-09-01 00:00:00

  • Vitamin D status and body mass index in children with atopic dermatitis: A pilot study in Italian children.

    abstract:BACKGROUND:Vitamin D (vitD) is involved in important regulatory functions of the innate and adaptive immune system. So, it has been hypothesized that vitD might influence the course of atopic dermatitis (AD). Also obesity may have impact on immune system. The aim of our study was to investigate vitamin D status and bod...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: D'Auria E,Barberi S,Cerri A,Boccardi D,Turati F,Sortino S,Banderali G,Ciprandi G

    更新日期:2017-01-01 00:00:00

  • Association of MMP-9 gene polymorphisms with Behçet's disease risk.

    abstract::The human matrix metalloproteinases (MMPs) are importantly involved in aneurysm formation. Since the clinical manifestations in Behçet disease (BD) include aneurysm formation among major symptoms, polymorphisms in MMP-9 might be associated with BD susceptibility. The aim of the current case-control study was to invest...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Naouali A,Kaabachi W,Tizaoui K,Amor AB,Hamzaoui A,Hamzaoui K

    更新日期:2015-03-01 00:00:00

  • Interaction between viruses and monoclonal antibodies studied by surface plasmon resonance.

    abstract::An automated biosensor system designed for measuring molecular interactions in real time and without any labelling of the reactants has been used to study the interaction of two animal viruses (vaccinia virus and poliovirus) and two plant viruses (cowpea mosaic virus and tobacco mosaic virus) with monoclonal antibodie...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Dubs MC,Altschuh D,Van Regenmortel MH

    更新日期:1992-01-01 00:00:00

  • Expression of CD44 splice variants in metastatic and non-metastatic mouse tumour cell lines.

    abstract::Different splice variants of the CD44 cell-surface molecule have been linked to metastasis formation in several animal and human cancers. We have used metastatic CSML-100 and non-metastatic CSML-0 mouse adenocarcinoma cell lines to determine whether variant CD44 molecules could be implicated in the different behaviour...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Tkach T,Li E,Bestagno M,Burrone OR

    更新日期:1996-09-01 00:00:00

  • Attenuated nef DNA vaccine construct induces cellular immune response: role in HIV-1 multiprotein vaccine.

    abstract::HIV-1 positive patients generate Nef-specific CTL response, indicating that Nef is a potent immunogen. However, Nef is also known to down regulate the expression of CD4 and MHC-I molecules, thereby protecting virally infected target cells. We compared the immunogenicity of non-functional nef vaccine constructs to wild...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Majumder B,Gray B,McBurney S,Schaefer TM,Dentchev T,Mahalingam S,Reinhart TA,Ayyavoo V

    更新日期:2003-10-31 00:00:00

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    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Kao JY,Zhang M,Chen CM,Chen JJ

    更新日期:2005-11-15 00:00:00

  • Carbon monoxide ameliorates Staphylococcus aureus-elicited COX-2/IL-6/MMP-9-dependent human aortic endothelial cell migration and inflammatory responses.

    abstract::Staphylococcus aureus (S. aureus) can often lead to many life-threatening diseases. It has the ability to invade normal endovascular tissue. Acute inflammation and its resolution are important to ensure bacterial clearance and limit tissue injury. Carbon monoxide (CO) has been shown to exert anti-inflammatory effects ...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Tsai MH,Yang CM,Chang KT,Chuang CC,Lin WN,Jiang RS,Wu CH,Lee IT

    更新日期:2018-11-01 00:00:00

  • Expression of IL-4 mRNA in peripheral blood mononuclear cells from normal donors in relation to expression of TLR2.

    abstract::When IL-4 mRNA was distinguished from mRNA encoding its antagonist, the splice variant IL-4delta2, it was found to correlate directly with expression of TLR2 in fresh peripheral blood mononuclear cells (PBMCs) from normal donors (p=0.0013). Similarly IL-4 mRNA was high when TLR2 mRNA was abundant compared to levels of...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Chang JS,Dheda K,Huggett JF,Kim LU,Zumla A,Rook GA

    更新日期:2006-08-15 00:00:00

  • Anti-ovalbumin antibody binds to 3T3 cells and induces DNA synthesis.

    abstract::Rabbit anti-ovalbumin antibodies were found to bind to Swiss NIH 3T3 fibroblasts by indirect immunofluorescence, and to induce DNA synthesis when these cells were arrested in G0 phase of their replicating cycle (G0-S activity). The proof that these effects were related to the ovalbumin paratopes is based on the follow...

    journal_title:Immunology letters

    pub_type: 杂志文章


    authors: Mangalo R,Doligez-Chambost A,Marcovich H

    更新日期:1987-05-01 00:00:00