Abstract:
:Mouse spleen cells were activated with IL2 for 4 days in the presence or absence of paraformaldehyde fixed YAC (PFY) tumor cells (spleen cell: PFY ratio 100:1). Fresh spleen cells had poor expression of Ly49A but the expression was significantly upregulated by IL2 activation. Addition of PFYs resulted in a further boosting of Ly49A expression. Besides Ly49A, the Ly49C receptors were also upregulated by PFYs. Upregulated Ly49 expression was not restricted to NK cells (NK1.1 positive cells) but was also seen on T cells (TCRbeta positive cells). Time kinetics studies indicated that maximum upregulation of Ly49 in response to PFY cells occurred on day 3 and 4 and the expression declined thereafter. Ly49 expression in response to PFYs was completely blocked if spleen cells were pre-treated with Mitomycin C, an inhibitor of DNA synthesis. These results show that the addition of a small number of paraformaldehyde fixed YAC cells to spleen cell cultures undergoing IL2 activation, resulted in a significant upregulation of Ly49 receptors and this process was dependent upon cell proliferative activity.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Das A,Saxena RKdoi
10.1016/s0165-2478(01)00206-1subject
Has Abstractpub_date
2001-06-01 00:00:00pages
73-7issue
2eissn
0165-2478issn
1879-0542pii
S0165-2478(01)00206-1journal_volume
77pub_type
杂志文章abstract::The effect of melphalan (L-PAM; L-phenylalanine mustard) administration on natural killer (NK) and natural cytotoxic (NC) activities of spleen cells from normal noninoculated BALB/c mice and mice bearing large MOPC-315 plasmacytoma tumors was investigated. Injection of L-PAM (7.5 or 15.0 mg/kg) had no effect on NK and...
journal_title:Immunology letters
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更新日期:2019-08-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:1998-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1989-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:2021-01-06 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.01.010
更新日期:2008-05-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2005.11.018
更新日期:2006-04-15 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2013.05.005
更新日期:2013-05-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90111-0
更新日期:1982-11-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.08.010
更新日期:2016-10-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.11.009
更新日期:2017-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00145-x
更新日期:1999-04-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90092-7
更新日期:1987-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00183-8
更新日期:2000-05-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.11.002
更新日期:2010-01-18 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Immunology letters
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更新日期:2003-03-03 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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更新日期:1983-03-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2021.01.007
更新日期:2021-01-25 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90160-x
更新日期:1987-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.02.002
更新日期:2009-03-24 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.10.004
更新日期:2020-12-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90090-2
更新日期:1985-01-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90201-z
更新日期:1990-09-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章,评审
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更新日期:1987-12-01 00:00:00