Abstract：
:Chagas' disease is accompanied by severe anemia and oxidative stress, which may contribute to mortality. In this study, we investigated the role of 5-lipoxygenase (5-LO) in the control of parasitism and anemia associated with oxidative damage of erythrocytes in experimental Trypanosoma cruzi infection. Wild-type C57BL/6, 129Sv mice treated or not with nordihydroguaiaretic acid (NDGA, 5-LO inhibitor), mice lacking the 5-LO enzyme gene (5-LO(-/-)) and inducible nitric oxide synthase gene (iNOS(-/-)) were infected with the Y strain of T. cruzi. Impairment of 5-LO resulted in increased numbers of trypomastigote forms in the blood and amastigote forms in the heart of infected mice. We assessed oxidative stress in erythrocytes by measuring oxygen uptake, induction time and chemiluminescence following treatment with tert-butyl hydroperoxide (TBH). Our results show that 5-LO metabolites increased lipid peroxidation levels in erythrocytes during the early phase of murine T. cruzi infection. NDGA treatment reduced oxidative damage of erythrocytes in C57BL/6 T. cruzi-infected mice but not in C57BL/6 iNOS(-/-) infected mice, showing that the action of NDGA is dependent on endogenous nitric oxide (NO). In addition, our results show that 5-LO metabolites do not participate directly in the development of anemia in infected mice. We conclude that 5-LO products may not only play a major role in controlling heart tissue parasitism, i.e., host resistance to acute infection with T. cruziin vivo, but in the event of an infection also play an important part in erythrocyte oxidative stress, an NO-dependent effect.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Borges CL,Cecchini R,Tatakihara VL,Malvezi AD,Yamada-Ogatta SF,Rizzo LV,Pinge-Filho Pdoi
10.1016/j.imlet.2009.02.002subject
Has Abstractpub_date
2009-03-24 00:00:00pages
38-45issue
1eissn
0165-2478issn
1879-0542pii
S0165-2478(09)00032-7journal_volume
123pub_type
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journal_title：Immunology letters
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(83)90063-9
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journal_title：Immunology letters
pub_type： 杂志文章,评审
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更新日期：2013-11-01 00:00:00
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journal_title：Immunology letters
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