Abstract：
:A variety of straightforward biochemical techniques have been applied to the analysis of HLA molecules. These techniques centre largely on two components: monoclonal antibodies (mAbs) and gel analysis systems. Although only the basic applications are discussed here, the techniques have broad applications. Monoclonal-gel systems may be used for the characterisation of polymorphisms in HLA class I alpha chains or class II alpha and beta chains. This requires the use of mAbs which are locus-specific and monomorphic. Alternatively, B lymphoblastoid cell lines (B-LCL) whose HLA antigen mobilities are known in advance may be used for screening uncharacterised monoclonals for locus specificity.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Lanchbury JSdoi
10.1016/0165-2478(91)90194-fsubject
Has Abstractpub_date
1991-07-01 00:00:00pages
23-9issue
1-2eissn
0165-2478issn
1879-0542pii
0165-2478(91)90194-Fjournal_volume
29pub_type
杂志文章,评审abstract：:Maturation of dendritic cells is a key step during induction of adaptive immune responses. Multiple pathways and factors are involved in the regulation of dendritic cell maturation. Alternative splicing of pre-mRNA, which is regulated by splicing factors, plays an important role in many biological processes, including...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.06.004
更新日期：2013-06-01 00:00:00
abstract：:Using embryonic chickens treated with testosterone propionate, the effects of congenital absence of the bursa of Fabricius determined by the frequency of J chain-positive cells was examined in the spleen, thymus and bone marrow at the embryonic and newly hatched stages. J chain-positive cells in the chicks without bur...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(84)90037-3
更新日期：1984-01-01 00:00:00
abstract：:The surface phenotypes of lymphoid cells in the developing embryonic thymus were characterized by using monoclonal antibodies. FT-2 antigen thus defined was predominantly expressed on thymocytes in the earlier embryonic stages in all the inbred mouse strains tested. The immunofluorescence and immunoperoxidase tests in...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90004-5
更新日期：1985-01-01 00:00:00
abstract：:In transplant rejection, graft versus host or autoimmune diseases T cells are mediating the pathophysiological processes. Compared to unspecific pharmacological immune suppression specific inhibition of those T cells, that are involved in the disease, would be an alternative and attractive approach. T cells are activa...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.06.002
更新日期：2009-08-15 00:00:00
abstract：:Nervous and immune systems share specific recognition molecules for signals that originate in both systems. The information substances are polypeptides and their receptors. They comprise the multi-directional information exchange network whereby brain and nervous system function including mood and emotion can be integ...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(87)90171-4
更新日期：1987-12-01 00:00:00
abstract：:When dimerized by Stem Cell Factor (SCF), the Receptor Tyrosine Kinase Kit triggers the proliferation of hematopoietic progenitors, including pro-B cells, and of some differentiated cells, including mast cells. We found previously that anti-Kit antibodies can mimic SCF and that anti-Kit-induced mast cell proliferation...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2012.02.007
更新日期：2012-03-30 00:00:00
abstract：:Several recent findings indicate that mouse bone marrow-derived mast cells (BMMC) express RNA encoding interleukin-4 (IL-4) and secrete detectable amounts of IL-4 in response to cross-linking of Fc epsilon RI receptors, Fc gamma R receptors, or to ionomycin. We investigated the desensitization of BMMC from the view po...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90122-8
更新日期：1994-07-01 00:00:00
abstract：:Using the method of estimation of alpha-helical and beta-folded types of secondary structure from amino acid composition, it has been established that interferons contain about 60-70% of alpha-helices and not more than 10-20% of beta-structure. The same result was obtained using empirical and stereochemical rules for ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(82)90070-0
更新日期：1982-01-01 00:00:00
abstract：:The inflammatory response plays an important role during the induction of several neonatal diseases. Previous studies have shown that during newborn infections, the natural imbalance between pro- and anti-inflammatory responses shifts toward the production of pro-inflammatory cytokines. In this study, we employed an a...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2015.12.004
更新日期：2016-02-01 00:00:00
abstract：:Linoleic and arachidonic acid concentrations in neutrophils from rats maintained on a diet with only 0.3% of the energy content as essential fatty acid (EFA, EFAD group) were reduced by 70 +/- 2.2% and 34.8 +/- 5.2%, respectively, compared with controls fed a diet with a normal 3% EFA content. Neutrophil chemiluminesc...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(86)90053-2
更新日期：1986-10-01 00:00:00
abstract：:Leukaemic blood lymphocytes from patients with B prolymphocytic leukaemia express the common acute lymphoblastic leukaemia antigen (CALLA). The in vitro incubation of prolymphocytes with leucocyte conditioned medium produced the acquisition of plasma cell morphology, of intracytoplasmic immunoglobulins and the simulta...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90005-7
更新日期：1985-01-01 00:00:00
abstract：:CD103(+)CD11b(+) dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103(+)CD11b(+) DC...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.05.012
更新日期：2016-08-01 00:00:00
abstract：:NADPH oxidase 2 is a superoxide-generating enzymatic complex based on the catalytic subunit gp91phox that is also known as Nox2. Initially identified in neutrophils, NADPH oxidase 2 was long considered responsible only for the killing of phagocytized microorganisms. However, advances in knowledge about redox signallin...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2020.02.009
更新日期：2020-05-01 00:00:00
abstract：:The peripheral B-cell pool is in dynamic equilibrium and is controlled by a variety of factors. The rate of generation of B cells can influence both the composition and size of the peripheral B-cell compartment. Mice deficient for lambda5 gene expression have a block in early B-cell development leading to a markedly r...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2005.05.013
更新日期：2005-11-15 00:00:00
abstract：:Natural anti-cholesterol antibodies (ACHAs) exist in mammalian species, moreover their level sensitively changes in pathological situations, such as atherosclerosis or HIV infection. The conditions of their production and functional role, however, still remained ill defined. Recently we developed IgG3 type monoclonal ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2012.01.009
更新日期：2012-03-30 00:00:00
abstract：:The immunosuppressant rapamycin is known to be a potent inhibitor of cytokine driven proliferation of T-lymphocytes and other cell types. Here we have examined the effect of rapamycin on the myeloblastic cell line M1 which responds to interleukin-6 (IL-6) with growth arrest and monocytoid differentiation. Single-agent...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00110-7
更新日期：1997-11-01 00:00:00
abstract：:Several studies have implicated a role for the cell surface glycoprotein CD44 in lymphocyte differentiation, adhesion to the matrix, homing, activation and apoptosis. However, the requirement of CD44 in B cell maturation remains elusive. To address this point, we analyzed the generation and activation of B lymphocytes...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2004.06.004
更新日期：2004-08-15 00:00:00
abstract：:B cell activating factor belonging to the TNF family (BAFF, also called BLyS, TALL-1, THANK, or zTNF4) is an important survival factor for B cells, and is able to regulate T cell activation. Recently, we have demonstrated that human soluble BAFF (hsBAFF) potently elevates NK cell activity of murine splenic cells in vi...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2008.07.005
更新日期：2008-10-30 00:00:00
abstract：:Cells engulf particles larger than 0.5 μm in diameter by phagocytosis, which is driven by cytoskeletal rearrangements. Phagocytosis by alveolar epithelial cells (AECs) helps to maintain the alveolar homeostasis. Yes-associated protein (YAP), a transcriptional coactivator of the Hippo pathway, affects proliferation, di...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2020.06.009
更新日期：2020-09-01 00:00:00
abstract：:To explore the genetic association of single nucleotide polymorphisms (SNPs) in the coding region of the NOTCH4, exon 3 C+1297T and exon 5 A+3063G, in a case-control analysis of 58 rheumatoid arthritis (RA) and 98 alopecia areata (AA) and 100 ethnically matched healthy subjects. NOTCH4 polymorphisms were genotyped by ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.01.002
更新日期：2013-02-01 00:00:00
abstract：:Interleukin 2 (IL 2) is a lymphocyte growth factor capable of inducing the rapid growth of antigen- or mitogen-stimulated T cells. To understand the molecular mechanisms of IL 2 action, we studied the synthesis of cellular polypeptides induced by IL 2. For a homogeneous cell population responsive to IL 2, we used a mu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90115-4
更新日期：1985-01-01 00:00:00
abstract：:The experimental infection of macaques with simian immunodeficiency virus (SIV) has provided strong evidence that it may be possible to develop a vaccine against AIDS. Live attenuated SIV vaccines have been found to confer the most potent protection against challenge with a variety of pathogenic viruses. This article ...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/s0165-2478(98)00153-9
更新日期：1999-03-01 00:00:00
abstract：:The effect of priming with various antigens on subsequent vaccination with the pneumococcal conjugate vaccine (CPV) was determined using BALB/c mice. Priming with pneumococcal polysaccharide or cross-reactive polysaccharide did not inhibit the IgG response to CPV immunization. Additionally, live intranasal colonizatio...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.04.013
更新日期：2006-08-15 00:00:00
abstract：:Molecular chaperones (heat shock proteins, Hsp-s) play a pleiotropic role in immunological functions. Hsp-s participate in the presentation of peptide antigens, folding of several immunologically important proteins, such as the MHC, and in the maintenance of the activation-competent conformation of key signaling molec...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2003.11.025
更新日期：2004-03-29 00:00:00
abstract：:DC are unique antigen presenting cells, and their ability to induce proliferation of T cells in a mixed leukocyte reaction (MLR) assay is commonly used for the evaluation of their function. To determine the mechanisms involved in DC-induced T cell activation in a primary MLR assay, a variety of different agents were e...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(01)00235-8
更新日期：2001-09-03 00:00:00
abstract：:Lymphocytes obtained from thymus and spleens of 1-6 week old White Leghorn cockerels, untreated or immunized twice with sheep red blood cells (SRBC), were cultured with common T-cell mitogens in serial dilutions and/or different concentrations of bovine prolactin (PRL). [3H]Thymidine incorporation in newly synthesized...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90044-q
更新日期：1990-06-01 00:00:00
abstract：:We report here that Trichosanthin (Tk), a primary active component isolated from a Chinese traditional medicinal herb, Trichosanthes kirilowii, potently inhibits lymphocyte proliferative response in vitro. We found that Tk treatment increased production of the interleukins IL-4 and IL-10, while production of IL-2 and ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2007.03.008
更新日期：2007-05-15 00:00:00
abstract：BACKGROUND:Notch pathway is highly conserved across species and is involved in the regulation of cell differentiation and activity both in embryonic development and adult life. Notch signaling has an important role in the development of hematopoietic stem cells and their differentiation to committed lineages, as well a...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2020.04.010
更新日期：2020-07-01 00:00:00
abstract：:Transplantation immunologists have long been intrigued by the natural allograft that results from normal mammalian pregnancy. Its general success contrasts with the rejection problems associated with most artifactual organ transplantation and raises intriguing questions concerning the nature of the mechanisms involved...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(88)90001-6
更新日期：1988-04-01 00:00:00
abstract：:Humanized mouse models present an important tool for preclinical evaluation of new vaccines and therapeutics. Here we show the human variable repertoire of antibody sequences cloned from a previously described human immune system (HIS) mouse model that possesses functional human CD4+ T cells and B cells, namely HIS-CD...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.06.001
更新日期：2017-08-01 00:00:00
