Abstract：
:This paper describes functional and genetic studies on the macrophage resistance gene Lsh/Ity/Bcg first described almost two decades ago. Working in vitro with resident peritoneal, liver (Kupffer cells) and bone marrow derived macrophages from congenic B10 (LshS) and B10.L-LshR mice it has been possible to demonstrate that the final effector mechanism for the gene in regulating antileishmanial activity involves production of reactive nitrogen rather than reactive oxygen intermediates. This in turn is dependent upon priming/activation of macrophages for enhanced TNF-alpha release which acts back on the macrophage in an autocrine manner to increase nitric oxide production. The precise point at which Lsh acts to control macrophage priming/activation has not been identified, but studies of early response gene expression show differences in KC mRNA levels at 2 h after LPS stimulation, and in c-fos mRNA as early as 20 min after stimulation with PMA plus ionophore, in peritoneal macrophages from congenic LshS and LshR mice. Data available suggest that both negative and positive signals may be involved in macrophage priming/activation, with LshS macrophages down-regulating their capacity for continued response to the autocrine loop. Work in progress will examine the role of TPA and cAMP response element-binding proteins in regulating gene expression in Lsh congenic mice. A major new initiative has also commenced to clone the Lsh gene by reverse genetics using yeast artificial chromosomes to walk towards Lsh from the closet proximal and distal markers on mouse chromosome 1.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Blackwell JM,Roach TI,Atkinson SE,Ajioka JW,Barton CH,Shaw MAdoi
10.1016/0165-2478(91)90032-6subject
Has Abstractpub_date
1991-10-01 00:00:00pages
241-8issue
2eissn
0165-2478issn
1879-0542pii
0165-2478(91)90032-6journal_volume
30pub_type
杂志文章,评审abstract：:A TNF-like factor was purified from lipopolysaccharide (LPS) induced New Zealand white rabbit serum. The TNF-like factor was purified by DEAE-Sephacel, Sephacryl S-200, Mono-Q, CM-affi gel Blue, Superose 12 H/R preparative columns to the specific activity of 4 x 10(6) U/mg protein. The purified protein was 45 kDa in i...
journal_title：Immunology letters
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更新日期：1998-02-01 00:00:00
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journal_title：Immunology letters
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更新日期：1989-02-01 00:00:00
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更新日期：1987-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1994-06-01 00:00:00
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journal_title：Immunology letters
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journal_title：Immunology letters
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更新日期：1996-06-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.01.005
更新日期：2006-05-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2020.08.002
更新日期：2020-11-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90098-1
更新日期：1994-02-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.07.013
更新日期：2014-11-01 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.06.001
更新日期：2006-08-15 00:00:00
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journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(93)90091-f
更新日期：1993-02-01 00:00:00
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更新日期：2010-02-16 00:00:00
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更新日期：2003-10-31 00:00:00
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更新日期：1984-01-01 00:00:00
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更新日期：1999-01-01 00:00:00
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更新日期：2009-04-27 00:00:00
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journal_title：Immunology letters
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更新日期：1996-01-01 00:00:00
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更新日期：1993-10-01 00:00:00
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更新日期：2001-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1988-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0165-2478(88)90049-1
更新日期：1988-06-01 00:00:00
abstract：:Retroplacental blood lymphocytes (RPL) obtained from women without complications at the term delivery were studied for proliferative response to mitogens and surface phenotype and were compared with autologous peripheral blood lymphocytes (PBL). Proliferation of RPL induced by phytohemagglutinin (PHA) was significantl...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(88)90042-9
更新日期：1988-03-01 00:00:00
