Abstract：
:Mimotopes derived from peptide phage display libraries may reproduce basic functions of epitopes including their antigenicity. In case of toxins, this property makes phage displayed mimotopes highly specific vaccine components free of the toxicity. To explore the potential of mimotopes for vaccine development, their ability of substituting the whole toxin molecule deserves a detailed characterization. We used mimotopes of noxiustoxin (NTX), a neurotoxin from scorpion Centruroides noxius, for studying its epitopes recognized by a panel of six monoclonal antibodies (mAbs), as well as their crossreactivity with homologous toxins from other species of the Centruroides genus. Although competitive (displacement) immunoassay showed that all six mAbs inhibit each other for binding to whole NTX molecule, the mimotopes used as specific probes allowed separation of the mAbs into two functional groups recognizing distinct non-overlapping epitopes mapped on the opposite sites of the three-dimensional structure of the toxin. The use of mimotopes permitted a precise specificity analysis of a panel of antibodies raised against this toxin, that may be very important for immunological characterization of other scorpion toxins and for vaccine development.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Hernández R,Gazarian TG,Hérion PS,Gazarian KGdoi
10.1016/s0165-2478(01)00320-0subject
Has Abstractpub_date
2002-02-01 00:00:00pages
97-103issue
2eissn
0165-2478issn
1879-0542pii
S0165247801003200journal_volume
80pub_type
杂志文章abstract：:Interferon inducible transmembrane protein (IFITM) family genes have been implicated in several cellular processes such as the homotypic cell adhesion functions of IFNs and cellular anti-proliferative activities. We previously showed that the IFITM3 single nucleotide polymorphisms (SNPs) associated with susceptibility...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2013.09.026
更新日期：2013-11-01 00:00:00
abstract：:Common γ chain (γC) cytokines, namely IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 are important for the proliferation, differentiation, and survival of lymphocytes that display antitumor activity, thus stimulating considerable interest for the use of cytokines in cancer immunotherapy. In this review, we will focus on the...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2015.11.007
更新日期：2016-01-01 00:00:00
abstract：:There is great interest in the antibodies-to-DNA transformation, since this change is characteristic of autoimmune diseases and contributes to its pathology. After immunization and fusions, 14 hybridomas bearing DNA-hydrolysis activity against pUC19 plasmid DNA were obtained. Genes coding for V(H) and V(L) regions of ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2010.01.004
更新日期：2010-03-10 00:00:00
abstract：:T cells recognize with a single receptor both a product of antigens processed by antigen presenting cells (APC1) and a self-marker molecule, encoded by the major histocompatibility complex (MHC, a property termed MHC-restricted recognition of antigen). During their differentiation in the thymus, T cells "learn" what t...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(90)90040-w
更新日期：1990-06-01 00:00:00
abstract：:Autoimmune hepatitis (AIH) is a chronic liver disease mediated by immunity, and could lead to liver fibrosis and hepatocellular carcinoma. However, the mechanisms for breaking hepatic tolerance and driving AIH still remain elusive. We herein reported that the non-specific liver inflammation triggered by carbon tetrach...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2019.11.010
更新日期：2020-02-01 00:00:00
abstract：:To investigate the in vivo role of complement component C5 it is common to compare the inflammatory response between C5-normal and C5-deficient inbred mice strains. Nevertheless, it should be expected that differences in the genetic backgrounds between those strains may affect several physiological parameters, complic...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.02.010
更新日期：2014-05-01 00:00:00
abstract：:Lethally irradiated (1000 rad) CBA/J mice were transplanted with anti-Thy 1 treated BALB/c bone marrow. Under these conditions, we uniformly observed the development of pathology suggestive of acute graft-vs.-host disease (GVHD), i.e. weight loss, diarrhoea, hypogammaglobulinemia and thymic hypoplasia. If, 2 wk before...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90109-9
更新日期：1985-01-01 00:00:00
abstract：:Protective immunity against Salmonella infection is known to require CD4 Th1 cells and B cells, but the role of MHC class-I-restricted CD8 T cells is less clear. Previous studies have suggested that CD8 T cells participate in secondary, but not primary, bacterial clearance. However, these studies have used experimenta...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2012.10.009
更新日期：2012-12-17 00:00:00
abstract：:CBA spleen cells have been stimulated in vitro with A/Jap influenza virus-infected CBA spleen cells to generate a 'primary' cytotoxic lymphocyte (CL) response. The culture conditions were devised to allow the segregation of individual clones of CL and cytotoxicity measured by the lysis of infected or non-infected L-92...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(82)90127-4
更新日期：1982-01-01 00:00:00
abstract：:The role of the immunomodulator Protein A (PA) (from Staphylococcus aureus, Cowan I strain) in the control of leishmanial infection was studied in experimental animals. Treatment of Leishmania donovani infected hamsters with PA led to a moderate level of reduction of parasite load in their spleen (68%) and liver (46%)...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(98)00102-3
更新日期：1999-02-01 00:00:00
abstract：:Work done in our laboratories, using a murine model, indicates that suppression of host immune responses might be due to secretion of soluble factors by tumor cells. The H238 cells (BALB/c embryonic fibroblasts transformed by UV-inactivated herpes simplex virus Type 2) exhibit progressive tumor growth with subsequent ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(91)90087-q
更新日期：1991-09-01 00:00:00
abstract：:Complement factor I (CFI) deficiency is typically associated to recurrent infections with encapsulated microorganisms and, less commonly, to autoimmunity. We report a 53-years old male who, in a routine control for non-alcoholic fatty liver disease, presented a flat beta-2 fraction at the capillary protein electropher...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.04.011
更新日期：2016-06-01 00:00:00
abstract：:A direct role for delayed hypersensitivity in organized granuloma formation has been emphasized in the literature, but some clinical and experimental studies have suggested a more complicated relationship. In order to further evaluate T-cell function in cutaneous granulomatous inflammation, hepatic granulomas isolated...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(86)90021-0
更新日期：1986-11-17 00:00:00
abstract：BACKGROUND:Omalizumab therapy was found to be safe and effective as an add-on therapy for patients with poorly controlled severe asthma. Although several studies over the last decade have demonstrated its efficacy in other Immunoglobulin E related diseases, its use in such conditions is off-label. OBJECTIVE:This study...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2020.08.002
更新日期：2020-11-01 00:00:00
abstract：:The phenomenon of virus neutralization is a function of three variables: the antibody (Ab), the virus and the target cell. Variation in any one of these parameters may drastically affect the results of assays for neutralization. In focusing on the virus as a variable in assays for the neutralization of HIV-1, it has b...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(96)02560-6
更新日期：1996-06-01 00:00:00
abstract：:A new method of panning for B lymphocytes is described in which the ability of the sIg+ cells to adhere depends on the nature and concentration of nonspecific protein used rather than on the use of anti-immunoglobulin. Rat lymph node cells were suspended in 3% bovine serum albumin in Tris-buffered Hanks' and incubated...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(87)90130-1
更新日期：1987-08-01 00:00:00
abstract：:The thymus contains a low frequency of B cells, about 0.1-0.5% of total population of thymocytes that were shown to contribute in thymic negative selection. The fact that B cells in the periphery have been contributed in the generation of the T regulatory cell compartment emerged the idea that this process may indeed ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.03.007
更新日期：2017-05-01 00:00:00
abstract：:In this study the effect of PHA activation on the phosphatase activity of CD45 has been investigated in human leukemic T-cell lines. It has been found that in vivo activation of the cells with PHA resulted in 2-4-fold increase in enzyme activity. Addition of PHA to the postnuclear supernatant of cell lysates also resu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90086-8
更新日期：1994-10-01 00:00:00
abstract：:The first step in the clearance of apoptotic cells is chemotactic migration of macrophages towards the apoptotic cells guided by find-me signals provided by the dying cells. Upon sensing the chemotactic signals, macrophages release ATP. ATP is then degraded to ADP, AMP and adenosine to trigger purinergic receptors con...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2017.02.002
更新日期：2017-03-01 00:00:00
abstract：:Treatment of purified human C5 with hydrogen peroxide leads to a reduction of functional activity in the classical immune haemolysis assay. The effect of H2O2 is enhanced by traces of iron-EDTA, and becomes even stronger when ascorbic acid is present in addition. The enhancement by iron and protective effects of vario...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(87)90103-9
更新日期：1987-02-01 00:00:00
abstract：:The aim of this work was to raise allotype specific monoclonal antibodies to human CD45, with the long-term objective of producing a reagent which could be used to prolong graft survival in renal transplantation through removal of passenger leukocytes from the graft. At present there are no anti-CD45 monoclonal antibo...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00056-5
更新日期：1999-06-01 00:00:00
abstract：:An autoreactive T lymphocyte clone, designated as F1C4 was established from an autoimmune mouse strain (NZB/NZW)F1. This clone proliferated in the presence of mitomycin C-treated splenic adherent cells (MMC-SAC) from syngeneic mice. This response was dependent on the numbers of MMC-SAC. The specificity of F1C4 for I-A...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(88)90175-7
更新日期：1988-08-01 00:00:00
abstract：:CD103(+)CD11b(+) dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103(+)CD11b(+) DC...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2016.05.012
更新日期：2016-08-01 00:00:00
abstract：:Dendritic cells (DCs) differentiated in the presence of IL-10 preferentially induce regulatory T-cells and tolerance. Whether the tolerogenic properties displayed by these DCs (Tol-DCs) can be overcome has not been fully explored. Here we show for the first time that Tol-DCs express higher levels of TLR5 mRNA, but not...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.06.007
更新日期：2009-08-15 00:00:00
abstract：OBJECTIVE:Behçet's disease (BD) is a chronic multi-factorial inflammatory disease with the important role of genetic in activation of its inflammatory response. Interleukin (IL)-33 is a member of the IL-1 family of cytokines that affects innate and adaptive immune systems to promote inflammatory responses. In the curre...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2018.11.005
更新日期：2019-08-01 00:00:00
abstract：:As shown earlier, the cells transformed in vitro by several different oncogenes, or spontaneously, during in vivo growth in normal hosts would be gradually replaced by the highly-tumorigenic descendants co-expressing high H2O2-catabolizing and PGE2-releasing activities. Acquisition of (H2O2(CA) + PGE(S)) phenotype pro...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00123-6
更新日期：1999-10-01 00:00:00
abstract：:The expression of antigen presenting MHC class I molecules can be enhanced through cytokines, e.g. upon infection with bacteria or viruses, either directly by enhancing class I gene transcription or by increasing the amounts of accessory proteins of the loading complex. Tapasin plays a significant role in the peptide ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00104-9
更新日期：2002-10-01 00:00:00
abstract：:In early seventies "natural killer (NK) cells", a third lymphocyte subset was discovered that revealed an unexpected ability to kill syngeneic and allogeneic tumor targets, thus emerging as the most potent non-specific cytotoxic cells in both human and mouse. Decades of research revealed the multifaceted nature of the...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2013.12.009
更新日期：2014-10-01 00:00:00
abstract：:NK-like T cells which express the NK1.1 molecule and CD3 (or TCR) of intermediate level (CD3int or TCRint cells) were recently demonstrated to be present in various immune organs, and to have NK-like cytotoxic activity against NK target cells. In this study, we investigated whether NK1.1- T cells could express NK1.1. ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(98)00014-5
更新日期：1998-04-01 00:00:00
abstract：:When IL-4 mRNA was distinguished from mRNA encoding its antagonist, the splice variant IL-4delta2, it was found to correlate directly with expression of TLR2 in fresh peripheral blood mononuclear cells (PBMCs) from normal donors (p=0.0013). Similarly IL-4 mRNA was high when TLR2 mRNA was abundant compared to levels of...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.06.001
更新日期：2006-08-15 00:00:00
