Abstract:
:An effective HIV vaccine will likely need to induce potent and broad-based immunity, including CD8+ T cell responses. Hence, a quantitative assay to measure such responses in animal models will be important in the evaluation of candidate HIV vaccines. We show here that a single immunization with HIV DNA vaccines, followed by challenge with recombinant vaccinia virus expressing the relevant HIV antigen, allows quantitative assessment of CD8+ T cell responses. These responses can be profound (>30% of total CD8+ T cells) and directly reflect the level of memory CD8+ T cells at the time of challenge. Therefore, this assay will facilitate the selection of promising HIV vaccine candidates for further evaluation.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Otten GR,Chen M,Doe B,zur Megede J,Barnett S,Ulmer Jdoi
10.1016/s0165-2478(02)00237-7subject
Has Abstractpub_date
2003-01-22 00:00:00pages
215-22issue
2eissn
0165-2478issn
1879-0542pii
S0165247802002377journal_volume
85pub_type
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