Abstract:
:Cellular membranes contain microdomains known as 'lipid rafts' or detergent-insoluble microdomains (DRM), enriched in cholesterol and sphingolipids. DRM can play an important role in many cellular processes, including signal transduction, cytoskeletal organization, and pathogen entry. Many receptors like T cell receptors, B cell receptors and IgE receptors have been shown to reside in DRM. The majority of these receptors depend on multivalent ligand interaction to associate with these microdomains. We, here, study association between the high affinity IgG receptor, FcgammaRI (CD64), and membrane microdomains. FcgammaRI is a 72kDa type I glycoprotein that can mediate phagocytosis of opsonized pathogens, but can also effectively capture small immune complexes, and facilitates antigen presentation. We found FcgammaRI to predominantly reside within detergent-insoluble buoyant membranes, together with FcRgamma-chain, but independent of cross-linking ligand. With the use of confocal imaging, FcgammaRI was found to co-patch with GM1, a microdomain-enriched glycolipid. Depletion of cellular cholesterol, furthermore, modulated FcgammaRI-ligand interactions. These data indicated FcgammaRI to reside within lipid rafts without prior triggering of the receptor.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Beekman JM,van der Linden JA,van de Winkel JG,Leusen JHdoi
10.1016/j.imlet.2007.12.003subject
Has Abstractpub_date
2008-03-15 00:00:00pages
149-55issue
2eissn
0165-2478issn
1879-0542pii
S0165-2478(07)00328-8journal_volume
116pub_type
杂志文章abstract::Coelomic fluid from the earthworm Lumbricus terrestris is known to contain immunoactive cells and molecules involved in immune defense. To assess the ability of such molecules to form precipitating complexes with anti-human immunoglobulins, radial immunodiffusion experiments were conducted. Anti-human IgG, anti-human ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90081-c
更新日期:1993-02-01 00:00:00
abstract::CD103(+)CD11b(+) dendritic cells (DC) are the major migratory DC subset in the small intestine lamina propria (siLP) and their survival is dependent on the transcription factor interferon regulatory factor 4 (IRF4). Mice with a DC-specific deletion of irf4 (CD11c-cre.Irf4 mice) have reduced mucosal CD103(+)CD11b(+) DC...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2016.05.012
更新日期:2016-08-01 00:00:00
abstract::The murine pre-B-like cell line, 70Z /3, has been shown to undergo differentiation in response to supernatants derived from concanavalin-A (Con A) stimulated rat spleen cells or ultraviolet light treated P388D1 macrophage cell line. After culture for 24 h with supernatant factors, normally membrane IgM negative 70Z /3...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90085-3
更新日期:1984-01-01 00:00:00
abstract::SJL mice develop resistance against tolerance between the 9th and 25th wk of life. This resistance is linked with a loss of suppressor capacity in the thymus. We have shown here that contact photosensitivity (CPS) decreases as a function of age and that this is due to an age-dependent increase in suppressor capacity. ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90102-1
更新日期:1986-03-01 00:00:00
abstract::The role of the immunomodulator Protein A (PA) (from Staphylococcus aureus, Cowan I strain) in the control of leishmanial infection was studied in experimental animals. Treatment of Leishmania donovani infected hamsters with PA led to a moderate level of reduction of parasite load in their spleen (68%) and liver (46%)...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00102-3
更新日期:1999-02-01 00:00:00
abstract::Monoclonal antibodies (mAb) reacting with native (TA5, TB12) and denatured (T33, T35) plant toxin mistletoe lectin I (MLI) from Viscum album have been obtained. The interaction between mAbs and native toxin with ML isoforms (MLII, MLIII) has been investigated. An immunological cross-reaction has been shown to take pla...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)00181-p
更新日期:1995-01-01 00:00:00
abstract::Recent data provide evidence that antigen-specific CD4+ T-cell clones or antigen-activated T-cell lines can kill antigen-presenting cells (APC). We focused our studies on monocytes acting as APC in cultures of T cells freshly isolated from peripheral blood. The presence of monocytes in culture was monitored by their a...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(95)00051-6
更新日期:1995-06-01 00:00:00
abstract::IgE-mediated activation of basophil granulocytes and mast cells follows a bell-shaped dose-response curve. The decreased activation at supraoptimal allergen stimulation is thought to be associated with SH2-containing inositol-5'-phosphatase 1 (SHIP-1). SHIP-1 phosphorylation is inversely related to IgE-mediated releas...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2019.04.003
更新日期:2019-06-01 00:00:00
abstract::We have previously reported the production of a series of human monoclonal antibodies reacting with mouse mammary tumor viral antigens as well as the human counterpart HuMTV antigen. Against two of these antibodies (B11 and 4.6/6), anti-idiotypic antibodies were generated, which appeared to be the internal image of vi...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(91)90128-w
更新日期:1991-04-01 00:00:00
abstract::Incorporation of [3h]thymidine ([3H]TdR) into concanavalin A (Con A)-stimulated murine splenocytes was found to be enhanced by addition of certain concentrations of ethanol, 2-propanol and acetone. The alcohol/acetone-induced enhancement of the Con A response was found to be accompanied by an increase of the percentag...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90057-8
更新日期:1982-06-01 00:00:00
abstract:UNLABELLED:To study the pathogenic importance of the rheumatoid factor (RF) in rheumatoid arthritis (RA) and to identify genes differentially expressed in patients and healthy individuals, total RNA was isolated from peripheral blood mononuclear cells (PBMC) from eight RF-positive and six RF-negative RA patients, and s...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.03.018
更新日期:2004-05-15 00:00:00
abstract::A375 human melanoma cell cultures grown in the presence of TGF beta contained greatly reduced cell numbers and exhibited drastic alterations in cell morphology compared to the control cultures. Preincubation of the cells with the cytokine for only 18 h was sufficient to induce these changes irreversibly. Examination o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90040-u
更新日期:1992-07-01 00:00:00
abstract::T cell functions are regulated by multiple signaling cascades, including the MKK4-JNK (c-Jun NH2 terminal kinase) pathway. However, the mechanism regulating the MKK4-JNK axis in T cells remains unclear. Herein, we demonstrated that protein acetylation modulates JNK activity induced by T cell receptor (TCR) activation....
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.12.002
更新日期:2018-02-01 00:00:00
abstract::Replication-competent retroviruses are not known to encode or induce nuclear antigens that are immunogenic in their natural hosts. We describe here the detection of a human T-lymphotropic virus (type I and type II) associated nuclear antigen (HTLV-NA) by an anticomplement immunofluorescence assay. Antibody to HTLV-NA ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(86)90120-3
更新日期:1986-08-01 00:00:00
abstract::Natural anti-cholesterol antibodies (ACHAs) exist in mammalian species, moreover their level sensitively changes in pathological situations, such as atherosclerosis or HIV infection. The conditions of their production and functional role, however, still remained ill defined. Recently we developed IgG3 type monoclonal ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2012.01.009
更新日期:2012-03-30 00:00:00
abstract::The earliest T-cell precursor population in the adult mouse thymus (low CD4 precursors) may be divided into 85% of cells expressing surface Mel-14 (LECAM-1, the lymphocyte homing receptor) and 15% of cells which are Mel-14. To date, this is the only surface marker for which we have found this population to be heteroge...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90177-5
更新日期:1994-05-01 00:00:00
abstract::Peanut agglutinin (PNA), a D-galactose specific lectin, agglutinated 10-18% of lymphocytes isolated from tonsils of 3- to 6-yr-old children. PNA+ cells were found to be mainly B lymphocytes showing a 9.6 times higher specific activity of DNA polymerase alpha compared to the PNA- cells. The specific activity of deoxycy...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90040-x
更新日期:1987-06-01 00:00:00
abstract::Several recent findings indicate that mouse bone marrow-derived mast cells (BMMC) express RNA encoding interleukin-4 (IL-4) and secrete detectable amounts of IL-4 in response to cross-linking of Fc epsilon RI receptors, Fc gamma R receptors, or to ionomycin. We investigated the desensitization of BMMC from the view po...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90122-8
更新日期:1994-07-01 00:00:00
abstract::Human natural killer (NK) cell activity was not significantly affected by leukotriene (LT) B4 over a wide concentration range (10(-6)-10(-14) M), whether added directly to the NK cell assay or after preincubation of effector cells for 2 h with the drug before addition of Cr labelled K562 target cells. In addition, LTB...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90003-3
更新日期:1985-01-01 00:00:00
abstract::Interleukin-35 (IL-35) is a novel anti-inflammatory cytokine and has been shown to play an important role in maintaining immune homeostasis. However, the effect of IL-35 on human asthma remains unclear. The present study is to investigate the expression and significance of IL-35 in childhood asthma. Forty-one asthmati...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.06.002
更新日期:2014-11-01 00:00:00
abstract::Yolkin is a product of proteolytic degradation of vitellogenin, a protein contained in eggs' yolk, with already described procognitive properties. Here, we investigated effects of yolkin on the humoral and cellular immune response in mice, phenotype of cells from lymphoid organs and function of innate immunity cells. ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2020.01.003
更新日期:2020-04-01 00:00:00
abstract::Treatment of purified human C5 with hydrogen peroxide leads to a reduction of functional activity in the classical immune haemolysis assay. The effect of H2O2 is enhanced by traces of iron-EDTA, and becomes even stronger when ascorbic acid is present in addition. The enhancement by iron and protective effects of vario...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90103-9
更新日期:1987-02-01 00:00:00
abstract::Lymphocytes obtained from thymus and spleens of 1-6 week old White Leghorn cockerels, untreated or immunized twice with sheep red blood cells (SRBC), were cultured with common T-cell mitogens in serial dilutions and/or different concentrations of bovine prolactin (PRL). [3H]Thymidine incorporation in newly synthesized...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(90)90044-q
更新日期:1990-06-01 00:00:00
abstract::The immunological phenotyping of peripheral blood mononuclear cells (PBMC) was assayed in a series of 22 patients suffering from severe cancer-associated malnutrition. A marked decrease of the T-lymphocyte subsets (CD3+, CD4+, CD8+) and of the CD20+ B lymphocytes occurred; there was however an increased percentage of ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90079-4
更新日期:1987-05-01 00:00:00
abstract::Natural levels of goat antiphosphorylethanolamine (anti-PE) antibodies decreased after injection of purified human C-reactive protein (CRP). The concentration of anti-CRP antibodies followed a continuous increment from non-detectable levels to a plateau at 80 days after first injection. ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(88)90070-3
更新日期:1988-05-01 00:00:00
abstract::Clonal and oligoclonal populations of gammadelta T cells, with respect to the expression of T cell receptors for antigen (Tcr), have been shown to localize in normal and inflamed tissues. The mechanisms responsible for the tissue-selective accumulation of these subsets are still not known. gammadelta T cells are the p...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(98)00077-7
更新日期:1998-11-01 00:00:00
abstract::Histamine and putrescine (a precursor of polyamines) are formed by histidine decarboxylase (HDC) and ornithine decarboxylase (ODC), respectively. Within a few hours after injection of a lipopolysaccharide (LPS) into mice, HDC is induced in the liver, spleen, lung and bone marrow, and ODC is induced in the liver, splee...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90086-4
更新日期:1992-06-01 00:00:00
abstract::Modulation of the IgE response by PHA treatment in relation to animal sex was analysed. Male and female RF mice were immunized with ovalbumin in aluminum hydroxide gel and PHA was injected 1 or 2 days before immunization. Castrated mice were also immunized and received PHA on day -2. The results show that the IgE resp...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90056-3
更新日期:1987-10-01 00:00:00
abstract::Graft failure (GF) following bone marrow transplantation (BMT) in man is usually attributed to allograft rejection; however, other mechanisms of GF exist. Exposure of the developing allograft to viruses, myelotoxic drugs, and damage to the host-derived marrow stroma during graft-versus-host disease (GVHD) may all caus...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/0165-2478(91)90204-n
更新日期:1991-07-01 00:00:00
abstract::The binding of nascent human C3b (i.e. the fragment of C3 just after trypsin cleavage) to mouse peritoneal macrophages was demonstrated by immune adherence. Acceptor-bound C3b could be detected longer than 24 h on the cell membrane. The rosette formation and phagocytosis of SRBC coated with anti-SRBC rat IgG was inhib...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(83)90068-8
更新日期:1983-06-01 00:00:00