Abstract:
:Four murine monoclonal antibodies (mAbs) showing similar reactivity with a cell-wall extract and mannan preparation obtained from Candida albicans were examined for epitope specificity. An enzyme-linked immunosorbent assay (ELISA) was used to determine total mAb binding to extracted cell-wall antigen when each mAb was reacted alone or in competition with a second mAb. This analysis suggested that three mAbs recognized the same determinant, which differed from that recognized by the fourth. The reactivity of these mAbs was also examined by indirect immunofluorescence assay with both yeast and germ tube forms of the dimorphic fungus. The three mAbs assigned the same epitope-specificity by ELISA showed two different patterns of reactivity with immunofluorescence. This discrepancy is discussed with respect to the postulated structure of mannan and the method of analysis.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Chaffin WL,Morrow KJ Jrdoi
10.1016/0165-2478(89)90134-xsubject
Has Abstractpub_date
1989-07-01 00:00:00pages
7-11issue
1eissn
0165-2478issn
1879-0542pii
0165-2478(89)90134-Xjournal_volume
22pub_type
杂志文章abstract::The link between innate and adaptive host immune response to Mycobacterium tuberculosis (M.tb) is driven by dendritic cells (DC). In this study, we examined the ability of prevalent clinical strains from south India (S7, S10) and laboratory strain H37Rv (Rv) to induce maturation of monocyte derived dendritic cells (Mo...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.01.009
更新日期:2008-05-15 00:00:00
abstract::The quasimonoclonal (QM) mouse provides a model to analyze B cell selection because major B cell antigen receptors (BCR) are composed of the knockin V(H)DJ(H) 17.2.25 (V(H)T) encoded H chain and the lambda1 or lambda2 L chain, thereby being specific for (4-hydoxy-3-nitrophenyl)acetyl (NP). We have reported that during...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.12.011
更新日期:2007-03-15 00:00:00
abstract::Within the 3' end of nucleotide sequence of human adult T-cell leukaemia virus (ATLV) four small open frames in various phases were found previously. The frames code for 10,000-, 11,000-, 12,000- and 27,000-dalton polypeptides, one of which can be responsible for virus transforming activity. By means of methods of pre...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90093-9
更新日期:1987-01-01 00:00:00
abstract::Several studies have implicated a role for the cell surface glycoprotein CD44 in lymphocyte differentiation, adhesion to the matrix, homing, activation and apoptosis. However, the requirement of CD44 in B cell maturation remains elusive. To address this point, we analyzed the generation and activation of B lymphocytes...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2004.06.004
更新日期:2004-08-15 00:00:00
abstract::Molecular chaperones (heat shock proteins, Hsp-s) play a pleiotropic role in immunological functions. Hsp-s participate in the presentation of peptide antigens, folding of several immunologically important proteins, such as the MHC, and in the maintenance of the activation-competent conformation of key signaling molec...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2003.11.025
更新日期:2004-03-29 00:00:00
abstract::CD27, which belongs to the TNF receptor family, is a costimulatory molecule that participates in T-cell activation. Unlike costimulatory molecules such as OX40 and 4-1BB, little is known about the role CD27 plays a role in the development of experimental diseases. We asked whether CD27 and its ligand CD70 participate ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2008.05.004
更新日期:2008-08-15 00:00:00
abstract::T helper17 (Th17) cells have been demonstrated to participate in the pathogenesis of hepatitis B virus (HBV) associated liver damage. However, the contribution of Th17 cells to immune activation and disease aggravation in patients with HBV infection is not fully clear. In this study, we investigated the Th17 cells fre...
journal_title:Immunology letters
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.imlet.2012.12.001
更新日期:2013-01-01 00:00:00
abstract::Mouse spleen cells were activated with IL2 for 4 days in the presence or absence of paraformaldehyde fixed YAC (PFY) tumor cells (spleen cell: PFY ratio 100:1). Fresh spleen cells had poor expression of Ly49A but the expression was significantly upregulated by IL2 activation. Addition of PFYs resulted in a further boo...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(01)00206-1
更新日期:2001-06-01 00:00:00
abstract::Administration of opioids that bind to the classical mu opioid receptor has been shown to lead to unintended alterations in immune function. Traditionally, altered immune function has been investigated with circulating immune cells. Effects of mu agonists on intestinal epithelial immune function have not been describe...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(02)00198-0
更新日期:2002-12-03 00:00:00
abstract::Over the last two decades HIV-1 has spread worldwide and has now surpassed malaria as the leading cause of infectious disease mortality in adults (http://www.who.int/infectious-disease-report/pages/ch1text.html). The clinical course and outcome of HIV-1 infection are highly variable among individuals. Most individuals...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/s0165-2478(01)00275-9
更新日期:2001-11-01 00:00:00
abstract::Silicosis is clinically characterized not only by respiratory disorders but by immunological abnormalities such as the appearance of autoantibodies and complications of autoimmune diseases. Dysregulation of apoptosis, particularly in the Fas/Fas ligand (FasL) pathway, has been considered to play a role in the pathogen...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(00)00177-2
更新日期:2000-05-01 00:00:00
abstract::IL-6 mediates its pleiotropic functions through two membrane proteins, a ligand-binding molecule (IL-6 receptor, IL-6R) and a non-ligand-binding signal transducer (gp130). Starting with a previously isolated cDNA clone encoding human gp130, recombinant soluble gp130 (sgp130) lacking the transmembrane and cytoplasmic r...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90138-e
更新日期:1992-02-01 00:00:00
abstract::Rabbit anti-ovalbumin antibodies were found to bind to Swiss NIH 3T3 fibroblasts by indirect immunofluorescence, and to induce DNA synthesis when these cells were arrested in G0 phase of their replicating cycle (G0-S activity). The proof that these effects were related to the ovalbumin paratopes is based on the follow...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90069-1
更新日期:1987-05-01 00:00:00
abstract::The immunomodulating properties of a neuropeptide hormone, corticotropin-releasing factor (CRF), led us to investigate its effect on cAMP production by human peripheral blood mononuclear cells (MNC). In response to stimulation with CRF (100 nM), a statistically significant (P = 0.019) increase occurred in the amount o...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(93)90189-9
更新日期:1993-03-01 00:00:00
abstract::The murine pre-B-like cell line, 70Z /3, has been shown to undergo differentiation in response to supernatants derived from concanavalin-A (Con A) stimulated rat spleen cells or ultraviolet light treated P388D1 macrophage cell line. After culture for 24 h with supernatant factors, normally membrane IgM negative 70Z /3...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(84)90085-3
更新日期:1984-01-01 00:00:00
abstract:AIM:To develop new approach for tumor immunotherapy with VEGFR2-based DNA vaccine. METHODS:VEGFR2 derived from different species were prepared by RT-PCR and subjected to recombinant constructs. The chimeric gene of VEGFR2 was prepared by integrating different epitopes in different species. By transfecting into cells, ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2009.07.013
更新日期:2009-09-22 00:00:00
abstract::The monoclonal antibody (mAb) UN2 was generated upon immunization of a Balb/c mouse with human thymocytes. mAb UN2 recognized an antigen expressed by a subpopulation of human thymocytes and peripheral blood lymphocytes. In thymus, mAb UN2 recognized cortical cells; its expression was higher on CD3bright than on CD3dim...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90035-3
更新日期:1994-09-01 00:00:00
abstract::It has been previously shown that normal human peripheral blood mononuclear cells produce a soluble factor(s) that causes selective inhibition of collagen production by cultured human diploid fibroblasts, and that stimulation of the mononuclear cells by appropriate mitogens greatly enhances its production. Because of ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90145-2
更新日期:1985-01-01 00:00:00
abstract::HLA-specific antibodies generated by allo-immunization are supposed to be involved in the control of HIV infections by both the neutralizing capacity of HLA-specific antibodies (Abs) and HLA-specific Ab-dependent complement-mediated lysis (CML). We further characterized CML of HIV primary isolates induced by HLA-speci...
journal_title:Immunology letters
pub_type: 信件
doi:10.1016/j.imlet.2009.08.005
更新日期:2009-09-22 00:00:00
abstract::In addition to expressing inhibitory cytokines and suppressive molecules, Treg cells could downplay inflammation by releasing cytotoxic molecules and eliminating proinflammatory immune cells. Colorectal cancer (CRC) is a common malignancy that has led to many cancer-related deaths. In this study, we investigated the c...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2019.10.007
更新日期:2020-01-01 00:00:00
abstract::Two chicken monoclonal antibodies (MAbs), HU/Ch2-7 and HU/Ch6-1, against heterophil Hanganutziu-Deicher (HD) antigens with N-glycolylneuraminic acid (NeuGc) at a terminal carbohydrate were established by cell fusions using chicken B cell lines lacking thymidine kinase and spleen cells from chickens immunized with II3N...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(92)90205-3
更新日期:1992-03-01 00:00:00
abstract::The effect of priming with various antigens on subsequent vaccination with the pneumococcal conjugate vaccine (CPV) was determined using BALB/c mice. Priming with pneumococcal polysaccharide or cross-reactive polysaccharide did not inhibit the IgG response to CPV immunization. Additionally, live intranasal colonizatio...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2006.04.013
更新日期:2006-08-15 00:00:00
abstract::The physiological role(s) of fetuin, a protein present in plasma and many tissues of developing animals at levels much higher than in the adult, is unknown. Here we show that fetuin can modify the responses of macrophages to lipopolysaccharide (LPS) stimulation. At concentrations of fetuin in the medium, corresponding...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/s0165-2478(97)00126-0
更新日期:1998-01-01 00:00:00
abstract::Administration of human recombinant interleukin-2 (RIL-2) into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of HeLa, HU 609T and T24B human carcinoma cells partially inhibited growth of the human tumor xenografts. In vitro activation of nu/nu spleen cells with human RIL-2 resulted in generation ...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(87)90013-7
更新日期:1987-04-01 00:00:00
abstract::H-2-linked gene(s) have been found to play a role in the quantitative regulation of response to human gamma-globulin (HGG) in mice selected for high or low antibody responsiveness to sheep erythrocytes. Unexpectedly, in a random genetically heterogeneous population of F2 interline hybrids, the gene(s) linked to the H-...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(82)90111-0
更新日期:1982-11-01 00:00:00
abstract::Multiple sclerosis (MS) is a debilitating neurological disorder of the central nervous system (CNS), characterized by activation and infiltration of leukocytes and dendritic cells into the CNS. In the initial phase of MS and its animal model, experimental autoimmune encephalomyelitis (EAE), peripheral macrophages infi...
journal_title:Immunology letters
pub_type: 杂志文章,评审
doi:10.1016/j.imlet.2014.03.006
更新日期:2014-07-01 00:00:00
abstract::The administration of protein antigens into earthworms Lumbricus terrestris and Eisenia foetida induces the formation of antigen-binding protein (ABP) with the maximum response occurring between days 4 and 8. High proteolytic activities observed both in coelomocytes and in coelomic fluids cause rapid antigen degradati...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(94)90145-7
更新日期:1994-07-01 00:00:00
abstract::This study aimed to investigate the influence of physical activity in innate immunity to conduce to an effective antitumoral immune response analyzing the phenotype and activation status of infiltrating cells. We analysed the intracellular cytokines and the transcription factors of tumor infiltrating lymphocytes (TILS...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2017.08.007
更新日期:2017-10-01 00:00:00
abstract::The surface phenotypes of lymphoid cells in the developing embryonic thymus were characterized by using monoclonal antibodies. FT-2 antigen thus defined was predominantly expressed on thymocytes in the earlier embryonic stages in all the inbred mouse strains tested. The immunofluorescence and immunoperoxidase tests in...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/0165-2478(85)90004-5
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND:The autoimmune regulator (AIRE) regulates autoimmunity and self-antigen expression, such as acetylcholine receptor (AchR), in the thymus. Regulatory T cells (Tregs) can down-regulate autoimmunity, but also promote tumor growth. The objective of this study was to examine the levels of AIRE, AchR, and Foxp3 ex...
journal_title:Immunology letters
pub_type: 杂志文章
doi:10.1016/j.imlet.2014.04.008
更新日期:2014-09-01 00:00:00