Metabotropic purinoreceptors in rat dorsal horn neurones: predominant dendritic location.

Abstract:

:Elevations of cytosolic free Ca2+ concentration ([Ca2+]i) induced by addition of ATP have been compared in rat dorsal horn neurones in slices and after their isolation. ATP application induced in neurones in situ a rise of [Ca2+]i by 201 +/- 12 nM. In Ca2+-free external solution the rise was 156 +/- 14 nM (n = 45 of 76), indicating the presence of active purinergic metabotropic receptors in about 59% of neurones. [Ca2+]i transients induced by 2MeSATP in Ca2+-free external solution were completely abolished by 10 microM PPADS, indicating that some of the corresponding receptors are of the P2Y1 type. In acutely isolated neurones which lost their dendrites, there were no metabotropic response. The results confirm the presence of metabotropic postsynaptic purinoreceptors located in the dendritic tree of dorsal horn neurones.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Kruglikov I,Shutov L,Potapenko E,Voitenko N,Kostyuk P

doi

10.1097/00001756-200111160-00026

subject

Has Abstract

pub_date

2001-11-16 00:00:00

pages

3503-7

issue

16

eissn

0959-4965

issn

1473-558X

journal_volume

12

pub_type

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