Abstract:
:The orientation of the mitotic spindle plays a key role in determining whether a polarized cell will divide symmetrically or asymmetrically. In most cell types, cytoplasmic dynein plays a critical role in spindle orientation. However, how dynein directs opposite spindle poles toward distinct and predetermined cell ends is poorly understood. Here, we show that dynein distributes preferentially to the spindle pole bodies (SPB) and astral microtubules (MTs) proximal to the bud in metaphase yeast cells. Dynein asymmetry depended on the bud neck kinases Elm1, Hsl1, and Gin4, on the spindle pole components Cnm67 and Cdk1, and on the B-type cyclins Clb1 and Clb2. Furthermore, phenotypic and genetic studies both indicated that dynein is unable to orient the spindle when it localizes to both poles and associated microtubules. Together, our data indicate that proper orientation of the spindle requires dynein to act on a single spindle pole.
journal_name
Dev Celljournal_title
Developmental cellauthors
Grava S,Schaerer F,Faty M,Philippsen P,Barral Ydoi
10.1016/j.devcel.2006.02.018keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
425-39issue
4eissn
1534-5807issn
1878-1551pii
S1534-5807(06)00115-8journal_volume
10pub_type
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