Abstract:
:Translation of ribosomal protein-coding mRNAs (RP-mRNAs) constitutes a key step in ribosome biogenesis, but the mechanisms that modulate RP-mRNA translation in coordination with other cellular processes are poorly defined. Here, we show that subcellular localization of RP-mRNAs acts as a key regulator of their translation during cell migration. As cells migrate into their surroundings, RP-mRNAs localize to the actin-rich cell protrusions. This localization is mediated by La-related protein 6 (LARP6), an RNA-binding protein that is enriched in protrusions. Protrusions act as hotspots of translation for RP-mRNAs, enhancing RP synthesis, ribosome biogenesis, and the overall protein synthesis in migratory cells. In human breast carcinomas, epithelial-to-mesenchymal transition (EMT) upregulates LARP6 expression to enhance protein synthesis and support invasive growth. Our findings reveal LARP6-mediated mRNA localization as a key regulator of ribosome biogenesis during cell migration and demonstrate a role for this process in cancer progression downstream of EMT.
journal_name
Dev Celljournal_title
Developmental cellauthors
Dermit M,Dodel M,Lee FCY,Azman MS,Schwenzer H,Jones JL,Blagden SP,Ule J,Mardakheh FKdoi
10.1016/j.devcel.2020.10.006subject
Has Abstractpub_date
2020-11-09 00:00:00pages
298-313.e10issue
3eissn
1534-5807issn
1878-1551pii
S1534-5807(20)30796-6journal_volume
55pub_type
杂志文章abstract::Nakada et al. revisit the controversial question of whether membrane lipids are able to diffuse from the axon of a neuron into the soma. Using single molecule imaging of a fluorescent phospholipid, the authors show that a diffusion barrier in the axon initial segment blocks the diffusion of lipids. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/s1534-5807(03)00240-5
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abstract::In this issue of Developmental Cell, Marhavý et al. (2011) uncover a transcription-independent molecular mechanism of interaction between auxin and cytokinin in the regulation of plant meristem function. By modulating endocytic trafficking of PIN1, cytokinin controls auxin flux and, therefore, auxin gradients. ...
journal_title:Developmental cell
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abstract::The extracellular matrix (ECM) plays a major role in cancer progression through its increased deposition and alignment. In this issue of Developmental Cell, Fattet et al. reveal a pathway in which ECM stiffness promotes EPHA2/LYN complex activation, leading to TWIST1 nuclear localization and triggering EMT in breast c...
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abstract::Cyclin E is a component of the core cell cycle machinery, and it drives cell proliferation by regulating entry and progression of cells through the DNA synthesis phase. Cyclin E expression is normally restricted to proliferating cells. However, high levels of cyclin E are expressed in the adult brain. The function of ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.08.009
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abstract::Drosophila neuroblasts sequentially express a set of four transcription factors that specify the fates of their progeny according to the successive order of their generation. In analogy to spatial coordinate genes that specify neuroblasts by position, these sequentially expressed genes can be called "temporal coordina...
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abstract::Localization of the actin crosslinking protein, alpha-actinin, to the cleavage furrow has been previously reported. However, its functions during cytokinesis remain poorly understood. We have analyzed the functions of alpha-actinin during cytokinesis by a combination of molecular manipulations and imaging-based techni...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2007.08.003
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abstract::Renewing tissues have the remarkable ability to continually produce both proliferative progenitor and specialized differentiated cell types. How are complex milieus of microenvironmental signals interpreted to coordinate tissue-cell-type composition? Here, we investigate the responses of intestinal epithelium to indiv...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2020.12.020
更新日期:2021-01-15 00:00:00
abstract::The Frizzled (Fz) receptor is required cell autonomously in Wnt/beta-catenin and planar cell polarity (PCP) signaling. In addition to these requirements, Fz acts nonautonomously during PCP establishment: wild-type cells surrounding fz(-) patches reorient toward the fz(-) cells. The molecular mechanism(s) of nonautonom...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.08.004
更新日期:2008-09-01 00:00:00
abstract::The Rho GTPase Cdc42 is essential for polarized growth of budding yeast. Temporal control of Cdc42 depends partly on the activity of its GTPase-activating proteins (GAPs). In this issue of Developmental Cell, Saito et al. report that Cdc42 GAP activity is regulated by the phospholipid composition of the bud-tip membra...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2007.10.008
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journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.07.022
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abstract::COPII vesicles assemble at ER subdomains called transitional ER (tER) sites, but the mechanism that generates tER sites is unknown. To study tER biogenesis, we analyzed the transmembrane protein Sec12, which initiates COPII vesicle formation. Sec12 is concentrated at discrete tER sites in the budding yeast Pichia past...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(04)00129-7
更新日期:2004-05-01 00:00:00
abstract::Macropinocytosis coordinates non-specific uptake of macromolecules and fluid. Cancers employ macropinocytosis to obtain nutrients to support their metabolic homeostasis. In this issue of Developmental Cell, Lee et al. (2019) report that glutamine deprivation boosts macropinocytosis via EGFR signaling induction, provid...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2019.07.020
更新日期:2019-08-05 00:00:00
abstract::Tissues have a natural capacity to replace dying cells and to heal wounds. This ability resides in resident stem cells, which self-renew, preserve, and repair their tissue during homeostasis and following injury. The skin epidermis and its appendages are subjected to daily assaults from the external environment. A hig...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2017.10.001
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abstract::The Golgi apparatus undergoes a ubiquitin-dependent disassembly and reassembly process during each cycle of cell division. Here we report the identification of the Golgi t-SNARE syntaxin 5 (Syn5) as the ubiquitinated substrate. Syn5 is monoubiquitinated by the ubiquitin ligase HACE1 in early mitosis and deubiquitinate...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2016.06.001
更新日期:2016-07-11 00:00:00
abstract::Blood vessels form an important interface between the environment and the organism by carrying oxygen and nutrients to all cells and thus determining cellular metabolism. It is therefore not surprising that oxygen and metabolism influence the development of the vascular network. Here, we discuss recent insights regard...
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pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2009.01.003
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journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.01.015
更新日期:2018-02-05 00:00:00
abstract::Prior to the pairing and recombination between homologous chromosomes during meiosis, telomeres attach to the nuclear envelope and form a transient cluster. However, the protein factors mediating meiotic telomere attachment to the nuclear envelope and the requirement of this attachment for homolog pairing and synapsis...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2007.03.018
更新日期:2007-06-01 00:00:00
abstract::DSL ligands promote proteolysis of the Notch receptor, to release active Notch intracellular domain (N(ICD)). Conversely, the E3 ubiquitin ligase Deltex can activate ligand-independent Notch proteolysis and signaling. Here we show that Deltex effects require endocytic trafficking by HOPS and AP-3 complexes. Our data s...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.09.002
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abstract::Caspase proteases execute apoptosis but also function in development. In this issue of Developmental Cell, Weaver et al. report that C. elegans CED-3 caspase promotes animal growth through PMK-1/p38 kinase cleavage, and at the expense of pathogen and stress immunity, revealing an unexpected homeostatic relationship be...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2020.04.006
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abstract::Little is known about the mechanisms governing neonatal growth and maturation of organs. Here we demonstrate that calcineurin/Nuclear Factor of Activated T cells (Cn/NFAT) signaling regulates neonatal pancreatic development in mouse and human islets. Inactivation of calcineurin b1 (Cnb1) in mouse islets impaired dense...
journal_title:Developmental cell
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doi:10.1016/s1534-5807(01)00025-9
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journal_title:Developmental cell
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journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.03.006
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abstract::Metastasizing breast carcinoma cells have been hypothesized to mobilize tissue-invasive activity by co-opting the proteolytic systems employed by normal mammary epithelial cells undergoing branching morphogenesis. However, the critical effectors underlying morphogenesis remain unidentified, and their relationship to b...
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abstract::MEF2 transcription factors are well-established regulators of muscle development. We have discovered an unanticipated role for MEF2C in the neural crest, where tissue-specific inactivation results in neonatal lethality due to severe craniofacial defects. We show that MEF2C is required for expression of the Dlx5, Dlx6,...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2007.03.007
更新日期:2007-04-01 00:00:00
abstract::The 6th European Meiosis Workshop, sponsored by EMBO and organized by Franz Klein of the University of Vienna, met in mid-September. More than 150 participants who have not discovered anything more interesting than sex gathered in a bucolic Alpine valley near the town of Obertraun, Austria, surrounded by granite massi...
journal_title:Developmental cell
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abstract::Cell constriction promotes epithelial sheet invagination during embryogenesis across phyla. However, how this cell response is linked to global patterning information during organogenesis remains unclear. To address this issue, we have used the Drosophila eye and studied the formation of the morphogenetic furrow (MF),...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2007.09.015
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abstract::Successive cell divisions during embryonic cleavage create increasingly smaller cells, so intracellular structures must adapt accordingly. Mitotic spindle size correlates with cell size, but the mechanisms for this scaling remain unclear. Using live cell imaging, we analyzed spindle scaling during embryo cleavage in t...
journal_title:Developmental cell
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abstract::Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Tax...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2015.10.001
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abstract::The mechanism responsible for subdividing the embryo into individual tissues is a fundamental, yet still poorly understood, question in developmental biology. Various general hypotheses have been proposed, involving differences in cell adhesion, contractility, or contact-mediated repulsion. However, the key parameter ...
journal_title:Developmental cell
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doi:10.1016/j.devcel.2013.09.004
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