Architecture and Characteristics of Bacterial Nanotubes.

Abstract:

:Bacteria display an array of contact-dependent interaction systems that have evolved to facilitate direct cell-to-cell communication. We have previously identified a mode of bacterial communication mediated by nanotubes bridging neighboring cells. Here, we elucidate nanotube architecture, dynamics, and molecular components. Utilizing Bacillus subtilis as a model organism, we found that at low cell density, nanotubes exhibit remarkable complexity, existing as both intercellular tubes and extending tubes, with the latter frequently surrounding the cells in a "root-like" fashion. Observing nanotube formation in real time showed that these structures are formed in the course of minutes, displaying rapid movements. Utilizing a combination of super-resolution, light, and electron microscopy, we revealed that nanotubes are composed of chains of membranous segments harboring a continuous lumen. Furthermore, we discovered that a conserved calcineurin-like protein, YmdB, presents in nanotubes and is required for both nanotube production and intercellular molecular trade.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Dubey GP,Malli Mohan GB,Dubrovsky A,Amen T,Tsipshtein S,Rouvinski A,Rosenberg A,Kaganovich D,Sherman E,Medalia O,Ben-Yehuda S

doi

10.1016/j.devcel.2016.01.013

subject

Has Abstract

pub_date

2016-02-22 00:00:00

pages

453-61

issue

4

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(16)00080-0

journal_volume

36

pub_type

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