Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin-2 and EFF-1 to Promote Membrane Repair in C. elegans.

Abstract:

:Membrane repair is essential for cell and organism survival. Exocytosis and endocytosis facilitate membrane repair in small wounds within a single cell; however, it remains unclear how large wounds in the plasma membrane are repaired in metazoans. Here, we show that wounding triggers rapid transcriptional upregulation and dynamic recruitment of the fusogen EFF-1 to the wound site in C. elegans epidermal cells. EFF-1 recruitment at the wounded membrane depends on the actin cytoskeleton and is important for membrane repair. We identified syntaxin-2 (SYX-2) as an essential regulator of EFF-1 recruitment. SYX-2 interacts with the C terminus of EFF-1 to promote its recruitment, facilitating both endoplasmic and exoplasmic membrane repair. Furthermore, we show that SYX-2-EFF-1 repair machinery acts downstream of the ESCRT III signal. Together, our findings identify a key pathway underlying membrane repair and provide insights into tissue repair and regenerative medicine after injury.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Meng X,Yang Q,Yu X,Zhou J,Ren X,Zhou Y,Xu S

doi

10.1016/j.devcel.2020.06.027

subject

Has Abstract

pub_date

2020-09-14 00:00:00

pages

624-638.e5

issue

5

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(20)30506-2

journal_volume

54

pub_type

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