Abstract：
:Like the thymus, the bursa of Fabricius is a site of massive lymphopoiesis accompanied by cell death in vivo. In the present study we have, therefore, examined whether chicken bursa and thymus cells exhibit apoptosis. Bursa and thymus cells from SC chickens, 4-10 weeks of age, were incubated for 8-24 hr with various reagents. Genomic DNA was isolated, electrophoresed in 3% Nusieve agarose gels, and examined for patterns of DNA fragmentation. A laddering of DNA in multiples of 200 base pairs, indicative of apoptosis, was observed particularly with bursa and, to a much smaller extent, with thymus or spleen cells. These patterns of DNA fragmentation from bursa cells could be prevented by adding phorbol myristate acetate (PMA) but not by its inactive analogue 4 alpha-PMA during culture. Ionomycin is not required for this effect and, alone, appears to be slightly toxic for bursa cells, although it does not inhibit the effect of PMA. PMA did not affect the degree of DNA fragmentation in spleen or thymus cells. The addition of the protein kinase C (PKC) inhibitor staurosporine abolished both the preventive effect of PMA on apoptosis and its protective effect on bursa cells, as assayed by [3H]thymidine incorporation 24-48 hr after the initiation of cultures. PKC inhibitors also prevented the proliferation-inducing effect of PMA + ionomycin on spleen cells. It is concluded that the activation of protein kinase C and perhaps other kinases protects against apoptosis in cultured bursa cells.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Asakawa J,Tsiagbe VK,Thorbecke GJdoi
10.1006/cimm.1993.1058subject
Has Abstractpub_date
1993-03-01 00:00:00pages
180-7issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(83)71058-0journal_volume
147pub_type
杂志文章abstract：:Activin A is a multifunctional factor of the transforming growth factor-beta (TGF-beta) superfamily and acts as an anti-inflammatory cytokine produced by microglia and macrophages. In this study, we investigated the regulatory effect and possible mechanism of activin A on activation of lipopolysaccharide (LPS)-induced...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.11.001
更新日期：2009-01-01 00:00:00
abstract：:Tumors harbor a population of cancer stem cells (CSCs) which can drive tumor progression and therapeutical resistance. Nature killer (NK) cells are best known for their ability to directly recognize and kill malignant cells. However, the susceptibility of cancer stem cells to NK cells is not fully understood. Here we ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.11.009
更新日期：2016-02-01 00:00:00
abstract：:CD48, the murine homolog of human CD58, binds to CD2 in rats and mice. Whereas inhibition of CD2 signaling leads to profound immunosuppression, no information is available on CD48-targeted therapy in the rat. We could show that anti-CD2 treatment (OX34) efficiently inhibited TCR-driven as well as CD2-mediated prolifer...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1204
更新日期：1997-11-25 00:00:00
abstract：:T-Cell subsets identified by polyclonal and monoclonal antibodies to dipeptidyl peptidase IV (DP IV) were investigated. Analysis in a cytofluorograf revealed 63 +/- 7% positive scatter-gated T lymphocytes. DP IV-positive cells were found to be T11+, 74-81% OKT4+, and 12-19% OKT8+. DP IV-negative cells were T11+ and co...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90400-9
更新日期：1985-06-01 00:00:00
abstract：:Neutrophils exhibit intrinsic sinusoidal metabolite concentration oscillations of 3 min in resting cells and an additional approximately 10- or 20-s oscillation in migrating/adhering cells. To better understand immune complex (IC)-mediated leukocyte activation, we have studied neutrophil metabolic oscillations in the ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1999.1481
更新日期：1999-05-25 00:00:00
abstract：:Aging leads to an increase in the proportion of cells that have the surface phenotype (CD45RBlo, CD44hi) characteristic of memory T lymphocytes and also to a decline in both the production of IL-2 and the response to this lymphokine. Several groups have reported an increase, with age, in the secretion of IL-4 in short...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1230
更新日期：1993-10-01 00:00:00
abstract：:The internalization of surface immunoglobulin (Ig) by B lymphocytes is the first step in the antigen-presenting function performed by these cells. Mature B cells coexpress on their surface IgM and IgD. At this time, there is controversy over whether these two isotypes serve different functions in the antigen-presentin...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90305-5
更新日期：1989-10-15 00:00:00
abstract：:We have studied the evolution of thyroiditis induced by neonatal thymectomy in Buffalo strain rats, with particular emphasis on the thyroid lymphocytic infiltrate. The earliest change was increased endothelial Ia expression, and infiltration of the thyroid at 5 weeks by ED1- and ED2-positive macrophages and B and T ce...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90260-2
更新日期：1988-06-01 00:00:00
abstract：:We recently reported that a cloned murine cell line with "natural killer (NK)-like" cytolytic function and prominent cytoplasmic granules also expressed large numbers of plasma membrane receptors (Fc epsilon R) which bound mouse immunoglobulin E (IgE) with high affinity (S.J. Galli et al., 1982, Nature (London) 298, 2...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90353-3
更新日期：1985-11-01 00:00:00
abstract：:Macrophages are highly plastic immune cells with temporally distinct transcriptome changes upon lipopolysaccride (LPS) activation. However, to what extent transcriptome reprogramming is mediated via spatial chromatin looping is not well studied. We generated high resolution chromatin interaction maps for LPS-stimulate...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2020.104148
更新日期：2020-09-01 00:00:00
abstract：:Oral administration of protein antigens, such as ovalbumin, may result in induction of either tolerance or immunization. To avoid oral tolerance, there are new strategies to protect the antigens from degradation within the gastrointestinal tract and to allow them to reach inductive immunological sites. One such strate...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.07.001
更新日期：2008-01-01 00:00:00
abstract：:We have previously reported that antibody secretion by B.22 hybridoma cells can be suppressed in an MHC-restricted manner, by idiotype-specific T cells. It was shown that T cells of both helper and suppressor phenotypes are involved, and that the suppression is mediated by soluble factors. In the present paper, we hav...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90288-8
更新日期：1989-10-15 00:00:00
abstract：:The primary objective of this study was to determine whether the n-3 PUFA-mediated changes in host response to a Listeria monocytogenes infection (e.g., cytokine production and bacterial clearance) were dependent upon neutrophils. Balb/c mice were fed one of two semi-purified diets that contained either 0 or 41 g of n...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2005.07.003
更新日期：2005-05-01 00:00:00
abstract：:We describe here a culture system for studying the development, in vitro, of antigen-specific CD4 T cells from unprimed mice. T cells from young mice are initially exposed to antigen, such as pigeon cytochrome C or keyhole limpet hemocyanin, in the presence of adherent accessory cells and then allowed to proliferate i...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1134
更新日期：1997-06-15 00:00:00
abstract：:Accumulating evidence indicates that inflammatory signals required for maximizing effector T cell generation have opposing effects on the development of memory T cell precursors. Toll-like receptor (TLR)2, and TLR9 significantly contribute to the inflammatory milieu and therefore in this study we examined whether the ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.12.009
更新日期：2016-04-01 00:00:00
abstract：:The effects of oral administration of methylated bovine serum albumin (mBSA) on antigen-induced arthritis (AIA) in rats that was induced by immunization with mBSA followed by the intraarticular injection of the same antigen were investigated. When mBSA was orally given to animals before immunization with the antigen, ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1098
更新日期：1995-06-01 00:00:00
abstract：OBJECTIVE:Many CD95-expressing cells don't always undergo apoptosis after stimulation with CD95 ligation. The purpose of this paper is to investigate the role of expression of CD95 (Fas/Apo1) on inflammatory response in fibroblast-like synoviocytes (FLS) obtained from rheumatoid arthritis (RA) and to evaluate the role ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2014.07.004
更新日期：2014-08-01 00:00:00
abstract：:Adoptive immunotherapy is a promising cancer treatment that entails infusion of immune cells manipulated to have antitumor specificity, in vitro. Antigen-specific cytotoxic T lymphocytes are the main executors of transformed cells during cancer immunotherapy. To induce antigen-specific cytotoxic T lymphocytes, we deve...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.10.002
更新日期：2018-12-01 00:00:00
abstract：:Multiple previous studies have demonstrated significant alterations of immunologic parameters associated with mouse hepatitis virus (MHV) infection, but effects of the virus on mucosal lymphoid cells have not been examined. Coincident with a natural outbreak of MHV at our institution, we noted alterations in immunoglo...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90295-4
更新日期：1987-10-01 00:00:00
abstract：:Using an in vitro model, we demonstrate that when CD4 T cells from HIV infected subjects are enriched from total blood lymphocytes the immune response to antigen is augmented. However, augmentation of this response is confined to HIV infected subjects with relatively preserved CD4 T cell counts. Enriching for CD4 T ce...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.09.003
更新日期：2006-07-01 00:00:00
abstract：:The effect of the addition of dialyzable leukocyte extract (DLE)(transfer factor) to tuberculostatic drugs in the treatment of superinfected fistulating tuberculosis of bones and joints was evaluated in a controlled study. Eleven patients whose disease had persisted for a mean of 20 +/- 4.8 years and had proved to be ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90091-1
更新日期：1984-03-01 00:00:00
abstract：:Two monoclonal antibodies specific for different rat T-cell subpopulations, the anti-helper-T-cell antibody, W3/25, and the OX8 suppressor cell antibody were used to investigate lectin-stimulated T-lymphocyte differentiation of F-344 rat bone marrow cells in culture. Cytofluorometric analysis of freshly isolated lymph...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90160-0
更新日期：1983-12-01 00:00:00
abstract：:Peripheral blood large granular lymphocytes (LGL) expressing Leu 11 (CD16) antigen with potent natural killer cytotoxicity inhibited soluble antigen-induced T-cell production of interleukin 2 (IL-2). Depletion of Leu 11-reactive cells from T-cells doubled IL-2 activity (P less than 0.05). Leu 11-enriched cells did not...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90389-4
更新日期：1989-02-01 00:00:00
abstract：:Epstein-Barr virus (EBV) readily immortalizes human peripheral blood lymphocytes (PBL) in vitro. However, during the past several years, we found that PBL from two exceptional EBV-seropositive healthy adult individuals were refractory to immortalization by EBV. We report here a study aimed at learning about the immuno...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90090-7
更新日期：1989-09-01 00:00:00
abstract：:OVA-specific T cells were immortalized by infection with radiation leukemia virus (RadLV). Some clones derived from such population were shown to exhibit helper activity. We then tested clones without such function and found among them some that secreted macrophage migration inhibition factor (MIF) and leukocyte migra...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90328-x
更新日期：1986-10-01 00:00:00
abstract：:Receptors for the Fc domain of IgG mediate target recognition, signal transduction, and effector functions including antibody-dependent cytolysis, phagocytosis, and phagolysosome formation. To better understand FcR-mediated functions and to identify potential therapeutic strategies, we employed cell-penetrating ("Troj...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.12.002
更新日期：2010-01-01 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSCs) play a major role in modulating immune response, but only a few reports focused on MDSCs in the liver of sepsis states. Here, we investigated the changes in MDSCs in liver of the cecal ligation and puncture (CLP) mice. The results of flow cytometry showed that MDSCs accumulate ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2012.08.005
更新日期：2012-09-01 00:00:00
abstract：:The effects of IL-10 on in vitro antigen-driven murine antibody responses and helper cell IL-4 and IFN-gamma secretory capacity were investigated. Low antigen concentrations stimulated high responses in all antibody isotypes examined; IgD was not assayed. Under these conditions, exogenous IL-10 minimally potentiated s...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0011
更新日期：1996-01-10 00:00:00
abstract：:The purpose of this study is to assess the potential of dendritic cells transfected with PD-L1 recombinant adenovirus induces CD8+ T cell suppression and kidney allograft tolerance. To prove it, DCs transfected with PD-L1 recombinant adenovirus (DC/Ad-PD-L1) were transferred into the MHC-mismatched rat kidney transpla...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.06.007
更新日期：2011-01-01 00:00:00
abstract：:Eighty-seven murine monoclonal antibodies (MAb) produced against human tumors of various origins and representing six different immunoglobulin classes were tested for antitumor reactivity in antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) assays. Mouse splenocytes, thio...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90068-1
更新日期：1985-04-15 00:00:00
