Abstract:
:Receptors for the Fc domain of IgG mediate target recognition, signal transduction, and effector functions including antibody-dependent cytolysis, phagocytosis, and phagolysosome formation. To better understand FcR-mediated functions and to identify potential therapeutic strategies, we employed cell-penetrating ("Trojan") peptides to deliver "wild-type" (LTL) or modified (AAA) FcgammaRIIA tail sequences to the neutrophil's cytoplasm. The Trojan-LTL peptide appeared to label the endoplasmic reticulum whereas the Trojan-AAA peptide distributed throughout the cytoplasm. The Trojan-LTL peptide, but not the Trojan-AAA peptide, decreased Ca(2+) signaling at the phagosome and reduced phagolysosome formation. These studies suggest that FcgammaRIIA's tail can act as a peptide decoy thereby blunting FcgammaRIIA-mediated processes, which, in turn, suggests a possible route in managing inflammatory tissue damage.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Clark AJ,Petty HRdoi
10.1016/j.cellimm.2009.12.002subject
Has Abstractpub_date
2010-01-01 00:00:00pages
153-8issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(09)00207-Xjournal_volume
261pub_type
杂志文章abstract::Murine bone marrow-derived cultured mast cells (BMMCs) are most widely used in in vitro experiments for evaluation of mast cell functions. The present study has shown that cell preparation procedure, i.e., cell collection by centrifugation and the subsequent adjustment and culture of cell density at the desired concen...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.01.001
更新日期:2005-11-01 00:00:00
abstract::Extracellular adenine nucleotides are inhibitors of the human natural killer cell line NK3.3 natural cytotoxicity activity. Natural cytotoxicity was inhibited approximately 26% by 1 mM ATP and 21% by 1 mM ADP. 5'-Adenylyl imidodiphosphate, a nonhydrolyzable ATP analog, inhibited natural cytotoxicity by 41% at a concen...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(95)80028-h
更新日期:1995-02-01 00:00:00
abstract::Treatment of human blood mononuclear cells with nanomolar concentrations of phorbol 12,13-dibutyrate (P(Bu)2) induced their aggregation. The phenomenon was seen within a few minutes and reached its maximum manifestation after 20 min. At this time, 20-30% of unfractionated and nylon wool-passed mononuclear leukocytes w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90313-1
更新日期:1983-01-01 00:00:00
abstract::It is suggested that autologous mixed lymphocyte reaction (AMLR) may play an important role in generating suppressor inducer signals and in down-regulating the immune response following self-major histocompatibility recognition. In the present study, monoclonal antibodies directed at cell surface structures on T4+ cel...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90270-x
更新日期:1987-06-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis (MS), and is induced by immunization with disease-causative self-antigens such as myelin oligodendrocyte glycoprotein (MOG). We have previously reported that transplantation of MOG expressing thymic epithelial progenitors (TEPs) ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.10.007
更新日期:2017-12-01 00:00:00
abstract::In view of the critical role played by bacillus Calmette-Guérin (BCG) in the development and functional activation of protective T cells against tuberculosis, it has become important to understand the mechanisms by which cytokines regulate BCG-mediated immune responses. There is evidence that cytokine-mediated suppres...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1662
更新日期:2000-06-15 00:00:00
abstract::In view of the eminently important role of interleukin-2 (IL-2) in T-cell responses, and in view of reports about immune stimulatory effects of PGE2, we reinvestigated the question whether PGE2 inhibits IL-2 production. It was found that PGE2 does not inhibit IL-2 production in murine spleen cell cultures after optima...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90180-7
更新日期:1985-01-01 00:00:00
abstract::The proliferative response of peripheral blood mononuclear cells (PBMC) in synthetic serum-free media depends on the presence of sufficient amounts of transferrin (Tf). In the present communication we show that the reduction of Tf concentration in culture media results in a decreased proliferation, whereas lymphokine ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90038-d
更新日期:1991-02-01 00:00:00
abstract::Stroke is the leading cause of disability in the United States. Sex differences, including smaller infarcts in females and greater involvement of immune-mediated inflammation in males may affect the efficacy of immune-modulating interventions. To address these differences, we sought to identify distinct stroke-modifyi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.06.003
更新日期:2017-08-01 00:00:00
abstract::Bispecific antibodies have been successfully introduced into clinical application. γδ T cells are of special interest for tumor immunotherapy, due to their recognition of pyrophosphates that are overproduced by many tumor cells resulting in HLA-nonrestricted tumor cell killing. Here we describe in detail a [(Her2)2 × ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.04.009
更新日期:2015-07-01 00:00:00
abstract::UM4D4 is a recently defined antigen that is expressed on approximately 25% of peripheral blood T cells, but on the majority of T cells in inflammatory synovial fluid. Anti-UM4D4 activates peripheral blood T cells in the presence of accessory cells and/or phorbol ester. UM4D4 has been assigned to a new antigen cluster ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90042-p
更新日期:1990-07-01 00:00:00
abstract::Essential thrombocythemia (ET), polycythemia vera (PV) and myelofibrosis share the same acquired genetic lesion, JAK2V617F. It is believed that cytokines participate in the activation of JAK2V617F. In this study, we analyzed the plasma levels of interleukin (IL)-23, IL-10 and IL-22 in patients with PV and ET. In the s...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.07.003
更新日期:2012-07-01 00:00:00
abstract::A decapeptide (1182-1191) derived from the bovine interphotoreceptor retinoid-binding protein (IRBP) was found to contain two completely distinct antigenic sites when tested in Lewis rats. One site, localized in sequence 1182-1191, is the core of the immunodominant and highly uveitogenic determinant of IRBP. The secon...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90181-n
更新日期:1992-03-01 00:00:00
abstract::We showed previously that the expressions of various src family protein tyrosine kinases (PTKs) were induced independently during the monocytic differentiation of HL-60 cells. The role of PTKs was further assessed in the present study by investigating the effects of PTK inhibitors on the differentiation. It was demons...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90196-v
更新日期:1992-04-01 00:00:00
abstract::Two interleukin-2 (IL-2)-dependent cytotoxic T-cell clones were obtained by limiting dilution from a lymphocyte culture stimulated in vitro with the autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) in the presence of fetal calf serum (FCS). Both clones uniformly had a T3+, T4+, Dr+ phenotype an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90304-7
更新日期:1986-04-01 00:00:00
abstract::Although it is well known that the CD3/T-cell receptor (TCR) complex modulates from the surface of T cells upon exposure to monoclonal antibodies (mAb) directed against it, the fate of bound mAb has not been yet elucidated. We therefore perform direct binding experiments of 125I-labeled mAb against CD3 or TCR to inves...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90209-2
更新日期:1988-10-01 00:00:00
abstract::Experimental autoimmune thyroiditis is induced in mice by immunization with thyroglobulin emulsified in Freund's complete adjuvant. The disease is characterized both by thyroid infiltration with mononuclear cells and by circulating thyroglobulin antibodies. The magnitude of the thyroid infiltration and the titer of th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90302-9
更新日期:1987-10-01 00:00:00
abstract::Upregulation of immunoglobulin D-specific receptors (IgD-R) on CD4+ T cells may facilitate their interaction with specific carbohydrate moieties uniquely associated with membrane IgD on B cells. Previous studies have shown that upregulation of IgD-R facilitates cognate T-B cell interactions by mediating bidirectional ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1747
更新日期:2001-02-01 00:00:00
abstract::Humans with autoimmune disease and autoimmune mouse strains such as NZB have been shown to produce reduced levels of the cytokines interleukin 1 and interleukin 2. The NZB X C58 recombinant inbred (N X 8 RI) strains exhibit certain of the autoimmune characteristics of the NZB strain. Their abilities to produce interle...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90177-1
更新日期:1984-10-15 00:00:00
abstract::Mammals and microorganisms have evolved a complex and tightly controlled mutual relationship. This interaction grants protection and energy source for the microorganisms, and on the other hand, provides several immunologic, metabolic and physiological advantages for the host. The gastrointestinal tract (GI) harbors th...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2018.01.017
更新日期:2018-08-01 00:00:00
abstract::Dendritic cell (DC)-based immunotherapy has promising for treatment of non-small cell lung cancer (NSCLC). Melanoma-associated antigen 3 (MAGE-A3) is a tumor-specific antigen and expressed in approximately 35-40% of NSCLC tissues. Calreticulin (CALR) is a protein chaperone and can enhance DC maturation and antigen pre...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.12.003
更新日期:2016-02-01 00:00:00
abstract::Sequential immunization with a carrier molecule and a new epitope (hapten) conjugated to the carrier (carrier/hapten-carrier immunization) induces specific suppression for IgG antibody production to the new epitope (hapten) on the carrier. Once induced, this "epitope-specific" suppression persists and specifically sup...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90387-3
更新日期:1984-07-01 00:00:00
abstract::CD8+ T cell differentiation is controlled by the transcription factors T-bet and Eomesodermin, in concert with the cytokines IL-2, IL-10 and IL-12. Among these pathways, the mechanisms by which T-box proteins and IL-10 interact to promote a memory T cell fate remain poorly understood. Here, we show that Eomes and IL-1...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.11.008
更新日期:2019-01-01 00:00:00
abstract::This study was conducted on human K562 lymphocytes to investigate the mechanisms implicated in the regulation of the serotonin transport process. The uptake of serotonin in these cells was saturable (Km, 3.37 microM; Vmax, 2.03 nmol/10(6) cells) and Na+ dependent; isoosmotic replacement of Na+ with choline chloride in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0242
更新日期:1996-09-15 00:00:00
abstract::It has been previously shown that the activated form of Factor B (Factor Bb) of the alternative pathway of complement activation stimulates monocyte spreading and killing of xenogenic erythrocytes and staphylococci. Factor Bb also stimulates lymphocyte blastogenesis in vitro, and native (uncleaved) Factor B is a major...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90005-x
更新日期:1985-05-01 00:00:00
abstract::Transcriptional regulation of gene expression is a key component of orchestrating proper immune cell development and function. One strategy for maintaining these transcriptional programs has been the evolution of transcription factor families with members possessing overlapping functions. Using the germ line deletion ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.02.009
更新日期:2015-05-01 00:00:00
abstract::Cyclosporin A (CsA) was tested for its modulatory effects on the mIgM-mediated signaling of G0*-associated increases in class II MHC expression, G1-related RNA synthesis, and S phase-related DNA synthesis in human B cells. While CsA at concentrations as low as 10-100 ng/ml could completely ablate anti-IgM-induced DNA ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90213-9
更新日期:1992-04-01 00:00:00
abstract::Interleukin (IL)-1 differs from most other cytokines by the lack of a signal sequence, which results in the retention of the immature proform intracellularly (i.c.). Several cell types have the capacity to produce IL-1, but release has been shown to be restricted predominantly to monocytes/macrophages and associated w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0113
更新日期:1996-05-01 00:00:00
abstract::Tumor-associated chemokines, including CC chemokine ligand 2/monocyte chemoattractant protein-1 (CCL2), are thought to play many roles in cancer progression. Here we demonstrate the novel finding that during growth of the D1-7,12-dimethylbenzanthracene-3 mammary tumor in BALB/c mice, there is a dramatic up-regulation ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.08.032
更新日期:2005-06-01 00:00:00
abstract::There are still questions about whether macrophage differentiation is predetermined or is induced in response to tissue microenvironments. C2D macrophage cells reside early in the macrophage lineage in vitro, but differentiate to a more mature phenotype after adoptive transfer to the peritoneal cavity (PEC-C2D). Since...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.012
更新日期:2011-01-01 00:00:00