Abstract：
:Transcriptional regulation of gene expression is a key component of orchestrating proper immune cell development and function. One strategy for maintaining these transcriptional programs has been the evolution of transcription factor families with members possessing overlapping functions. Using the germ line deletion of Snai2 combined with the hematopoietic specific deletion of Snai3, we report that these factors function redundantly to preserve the development of B and T cells. Such animals display severe lymphopenia, alopecia and dermatitis as well as profound autoimmunity manifested by the production of high levels of autoantibodies as early as 3 weeks of age and die by 30 days after birth. Autoantibodies included both IgM and IgG isotypes and were reactive against cytoplasmic and membranous components. A regulatory T cell defect contributed to the autoimmune response in that adoptive transfer of wild type regulatory T cells alleviated symptoms of autoimmunity. Additionally, transplantation of Snai2/Snai3 double deficient bone marrow into Snai2 sufficient Rag2(-/-) recipients resulted in autoantibody generation. The results demonstrated that appropriate expression of Snai2 and Snai3 in cells of hematopoietic derivation plays an important role in development and maintenance of immune tolerance.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Pioli PD,Chen X,Weis JJ,Weis JHdoi
10.1016/j.cellimm.2015.02.009subject
Has Abstractpub_date
2015-05-01 00:00:00pages
1-18issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(15)00043-Xjournal_volume
295pub_type
杂志文章abstract：:The loss of thymic function with age may be due to diminished numbers of T-cell progenitors and the loss of critical mediators within the thymic microenvironment. To assess the molecular changes associated with this loss, we examined transcriptomes of progressively aging mouse thymi, of different sexes and on caloric-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.03.008
更新日期：2007-01-01 00:00:00
abstract：:We have characterized in vivo and in vitro responses of mice to the BALB/c-derived carcinoma, C26. BALB/c mice were highly susceptible, in a dose-dependent fashion, to local tumor development following subcutaneous injection of C26. Other strains of mice, including allogeneic strains and major histocompatibility compl...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90160-3
更新日期：1989-08-01 00:00:00
abstract：:Dendritic cell (DC)-based immunotherapy has promising for treatment of non-small cell lung cancer (NSCLC). Melanoma-associated antigen 3 (MAGE-A3) is a tumor-specific antigen and expressed in approximately 35-40% of NSCLC tissues. Calreticulin (CALR) is a protein chaperone and can enhance DC maturation and antigen pre...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.12.003
更新日期：2016-02-01 00:00:00
abstract：:NK activity is regulated by arachidonic acid metabolites. More precisely PGE2 and LTB4 decreases and increases respectively non-MHC-restricted cytotoxicity in humans. We have observed similar data in mice since NK activity was inhibited by PGE2 (10(-6) to 10(-8) M) and enhanced by LTB4 (10(-8) to 10(-12) M). On the ot...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90117-8
更新日期：1992-01-01 00:00:00
abstract：:The third complementarity determining region (CDR3) is the most variable part of alpha beta T cell receptors (TCR) and represents the putative antigen contacting site. To identify parameters determining the structural diversity of the CDR3 region, the CDR3 length distributions of 66 BV-J combinations in peripheral CD4...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0071
更新日期：1996-03-15 00:00:00
abstract：:Inherited deficiency of the enzyme adenosine deaminase (ADA) has been found in a significant proportion of patients with severe combined immunodeficiency disease and inherited defect generally characterized by a deficiency of both B and T cells. Two questions are central to understanding the pathophysiology of this di...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90317-x
更新日期：1985-10-15 00:00:00
abstract：:The reactivity of human cord blood lymphocytes was assessed against a panel of monoclonal antibodies (MoAb). The mean proportion of OKT3+ cells (pan-T) was significantly lower in cord blood (52 +/- 13.8%; mean +/- SD) compared with that of adult blood (75 +/- 8.9%) and paralleled well with the E-rosette-forming capaci...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90209-0
更新日期：1984-11-01 00:00:00
abstract：:We have previously reported that antibody secretion by B.22 hybridoma cells can be suppressed in an MHC-restricted manner, by idiotype-specific T cells. It was shown that T cells of both helper and suppressor phenotypes are involved, and that the suppression is mediated by soluble factors. In the present paper, we hav...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90288-8
更新日期：1989-10-15 00:00:00
abstract：:This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34(+)CD38(-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.07.008
更新日期：2006-05-01 00:00:00
abstract：:Several lines of evidence suggest that nitric oxide (NO), generated through nitric oxide synthase (NOS) by cleavage of terminal guanidino nitrogen from L-arginine, mediates tumor cell killing by mononuclear phagocytes. Natural killer (NK) cells are cytotoxic effector cells that lyse a variety of tumor and virus-infect...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1215
更新日期：1994-08-01 00:00:00
abstract：:The transfer of B cells from mice immunized with Type III pneumococcal polysaccharide (SSS-III) results in the activation of suppressor and amplifier T cells that control the magnitude of the antibody response in recipient mice, immunized subsequently with SSS-III. Prior treatment of transferred B cells with an excess...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90368-5
更新日期：1985-12-01 00:00:00
abstract：:In nonactivated human monocytes, radiolabeled oleic, arachidonic, and palmitic acids are primarily incorporated into neutral lipids and phosphatidylcholine. Each of these fatty acids is also incorporated into phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, and sphingomyelin in characteristic propor...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90009-e
更新日期：1992-08-01 00:00:00
abstract：:Mice infected with virulent Mycobacterium tuberculosis exhibit an age-related increase in susceptibility to disease. The basis of this susceptibility has previously been shown to reflect an inability of the aged host to generate protective CD4 T cells during the early course of the infection. The results of the presen...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1062
更新日期：1993-03-01 00:00:00
abstract：:CD20 is expressed strictly by B-cells and is ubiquitously expressed at high surface densities of malignant human B-cells. This suggests that CD20 may be a tumor target for immunotherapy of B-cell lymphomas. Rituximab, a chimeric monoclonal antibody directed against CD20, has been demonstrated to be an effective treatm...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.05.005
更新日期：2006-02-01 00:00:00
abstract：:Activated macrophages are known to release a variety of immunoregulatory substances including the low-molecular-weight substances hydrogen peroxide and lactate. We report here that lactate but not hydrogen peroxide is capable of supporting a substantial production of T-cell growth factor (TCGF) in cultures of accessor...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90224-3
更新日期：1987-09-01 00:00:00
abstract：:Purified monomorphic monoclonal antibodies against Class I and Class II antigens in the inhibition of in vitro allogenic response were assayed. As expected, anti-Class II antibodies are highly inhibitory when used in concentrations greater than 5 micrograms/ml in MLRI and 50 micrograms/ml in MLRII. Surprisingly, anti-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90078-3
更新日期：1983-07-15 00:00:00
abstract：:Recently we reported on a novel H2E transgenic, IA-negative model of experimental autoimmune thyroiditis (EAT) that excludes reactivity to self in its susceptibility pattern to heterologous thyroglobulin (Tg). In conventional, susceptible mouse strains, EAT is inducible with both homologous and heterologous Tg; e.g., ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2001.1837
更新日期：2001-08-25 00:00:00
abstract：:Immunocytochemical staining procedures were combined with computer-aided image analysis to quantitate the relative intracellular production of tumor necrosis factor-alpha (TNF) within individual macrophages. Optimal conditions for time and methods for the activation of TNF production, fixation of cells for optimal imm...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90278-y
更新日期：1990-10-15 00:00:00
abstract：:In this study the influence of a non-mitogenic anti-CD3 antibody on accessory cell-dependent antigen and mitogen-induced T-lymphocyte proliferation has been investigated. The antibody was found to completely inhibit PHA, Con A, PWM, and tetanus toxoid stimulation, with no effect on the proliferation induced by the cal...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90014-6
更新日期：1986-06-01 00:00:00
abstract：:Two interleukin-2 (IL-2)-dependent cytotoxic T-cell clones were obtained by limiting dilution from a lymphocyte culture stimulated in vitro with the autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) in the presence of fetal calf serum (FCS). Both clones uniformly had a T3+, T4+, Dr+ phenotype an...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90304-7
更新日期：1986-04-01 00:00:00
abstract：AIMS:Non-enzymatic reaction of biomolecules leads to the formation of advanced glycation end products (AGEs). AGEs plays significant role in the pathophysiology of type 2 diabetes mellitus. Methylglyoxal (MG) is a highly reactive carbonyl compound which causes formation of early (ketoamines), intermediate (dicarbonyls)...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2020.104062
更新日期：2020-05-01 00:00:00
abstract：:Human mononuclear cells stimulated with soluble IgG aggregates generated chemiluminescence, a response attributable to monocytes. Some requirements of this reaction were examined by preincubation of the cells with a variety of inhibitors. The protease antagonists TPCK and TLCK, the phospholipase inhibitors quinacrine ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90149-7
更新日期：1984-08-01 00:00:00
abstract：:Ligation of CD40 using anti-CD40 or soluble CD40-ligand activates numerous intracellular kinases which transduce signals to the nucleus. The nature whereby these signaling events are coupled to distal functional events in B cells is poorly understood. In this study, using anti-CD40 monoclonal antibodies which recogniz...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2000.1645
更新日期：2000-05-01 00:00:00
abstract：:We systematically investigated the impact of the relative maturation levels of dendritic cells (DCs) on their cell surface phenotype, expression of cytokines and chemokines/chemokine receptors (by DNA array and RNase protection analyses), biological activities, and abilities to induce tumor immunity. Mature DCs expres...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2001.1883
更新日期：2001-11-25 00:00:00
abstract：:Microglia are important immunoregulatory cells within the central nervous system (CNS). Viral infection of primary microglia and splenic macrophage clones revealed that both exhibited a heterogeneous, but relatively low, sensitivity to cytolysis mediated by CD8(+) cytotoxic T lymphocytes (CTL). The majority of clones ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1999.1581
更新日期：1999-11-25 00:00:00
abstract：:The fine specificity of anti-Glu60Ala30Tyr10 (GAT) and anti-Glu60Ala40 (GA) proliferating cells was studied. T cells primed with GAT proliferate both to GAT and GA and GA-primed T cells proliferate also to GA and GAT. This cross-reactivity was unexpected given the results previously reported on the fine specificity of...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90309-x
更新日期：1983-01-01 00:00:00
abstract：:At concentrations as low as 10(-7) M, the cardiotonic glycosteroid ouabain, a specific inhibitor of the membrane Na+, K+-ATPase, is known to inhibit in vitro human lymphocyte proliferation produced in mixed lymphocyte cultures or induced by various stimulating agents (PHA, Con A, PWM, soluble antigens), while mouse ly...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90314-9
更新日期：1984-02-01 00:00:00
abstract：:The aim of the study was to investigate the interaction between manLAM and DC-SIGN influencing DCs maturation and downstream immune response using small interfering RNA-expressing lentiviral vectors to specifically knockdown DC-SIGN. Our data indicated that DC-SIGN knockdown alone in DCs did not affect the maturation ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.09.001
更新日期：2011-01-01 00:00:00
abstract：:The regulatory mechanism of guinea pig lymphokines was investigated in regard to differentiation of myeloid cells to macrophages. The Ml-cell line, established from a myeloid leukemia of an SL-strain mouse, was induced to differentiate in vitro into mature macrophages possessing Fc receptors and the ability to phagocy...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90355-6
更新日期：1983-02-15 00:00:00
abstract：:The activation by concanavalin A (Con A) of murine lymphocytes derived from aged animals is impaired, as demonstrated by lower IL-2 synthesis, display of fewer IL-2 receptors, and less [3H]thymidine incorporation relative to similarly treated cells from young animals. The ability of the phorbol ester, phorbol 12-myris...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90250-x
更新日期：1988-06-01 00:00:00
