Abstract:
:At concentrations as low as 10(-7) M, the cardiotonic glycosteroid ouabain, a specific inhibitor of the membrane Na+, K+-ATPase, is known to inhibit in vitro human lymphocyte proliferation produced in mixed lymphocyte cultures or induced by various stimulating agents (PHA, Con A, PWM, soluble antigens), while mouse lymphocyte proliferation is unaffected at this concentration. Ouabain inhibits most of proliferative response parameters at all stages of the transformation. This observation prompted us to suggest that ouabain could also act through inhibition of interleukin production which is known to occur during the first hours after T-cell stimulation in the presence of monocytes. In order to check the possible influence of ouabain on interleukin production, conditioned media from stimulated human mononuclear cells, prepared in the presence or in the absence of inhibitor, were tested for their ability to promote a mouse thymocyte response to PHA. Instead of the expected inhibition, we found that ouabain, even at high concentrations (2 X 10(-6) M) enhanced the stimulatory effect and/or the production of murine thymocyte activating factor(s). Moreover conditioned media from serum-free cultures of unstimulated human mononuclear cells exposed for 24 hr to low ouabain concentrations (10(-8) to 10(-7) M) showed a high activating effect on the response of murine thymocytes to PHA. This soluble factor produced upon ouabain treatment is produced by adherent cells and appears to be functionally similar to interleukin 1.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Dornand J,Favero J,Bonnafous JC,Mani JCdoi
10.1016/0008-8749(84)90314-9subject
Has Abstractpub_date
1984-02-01 00:00:00pages
351-9issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(84)90314-9journal_volume
83pub_type
杂志文章abstract::In this report, a subset of CD4(+) cells which kills autologous HLA class I positive and HLA class II negative non-small-cell lung cancer (NSCLC) cells is described. Killing was performed both by direct cytolytic mechanisms and by apoptosis. The peculiar characteristic of these effectors was their capability of lysing...
journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1027
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pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:Cellular immunology
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journal_title:Cellular immunology
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更新日期:1986-09-01 00:00:00
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