Impaired rearrangement of IgH V to DJ segments in bone marrow Pro-B cells from old mice.


:There are fewer bone marrow Pre-B cells in old compared to young mice. We have demonstrated both decreased rearrangement of the V to DJ IgH gene segments and low levels of VH germline transcripts in Pro-B cells, the precursors of Pre-B cells, from old compared to young mice. However, there was no difference in the level of RAG-mRNA in purified Pro-B cells from old and young mice. Consistent with the prior reports that fewer bone marrow emigrants enter the peripheral B cell populations of old than young mice, we identified fewer transitional B cells in the blood, as well as the spleen, of old than young mice. Association of impaired IgH rearrangement with a decreased number of transitional B cells in old mice was supported by finding that the percentage and number of transitional B cells expressing rearranged IgH and IgL transgenes, which do not require rearrangement of their endogenous IgH gene segments, were comparable in old and young mice. In contrast, the percentage and number of transitional B cells in these Ig-transgenic mice, which escaped allelic exclusion and have rearranged endogenous IgH gene segments, showed an age-associated decline similar to that seen in wild type mice. These data are consistent with the view that impaired V to DJ rearrangement contributes to the decreased levels of bone marrow Pre-B cells as well as the decreased levels of transitional B cells in the periphery.


Cell Immunol


Cellular immunology


Szabo P,Shen S,Telford W,Weksler ME




Has Abstract


2003-03-01 00:00:00














  • Downregulation of insulin-like growth factor-1 receptor (IGF-1R) expression in human T lymphocyte activation.

    abstract::Although it is known that IGF-1 increases T lymphocyte proliferation, the regulation of its receptor expression during the process has not been defined. Consequently the regulation of IGF-1R expression by IGF-1 and the activation events in human blood T lymphocytes and the Jurkat T cell line were now investigated. IGF...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Schillaci R,Brocardo MG,Galeano A,Roldán A

    更新日期:1998-02-01 00:00:00

  • Studies of the in vitro activation and differentiation of human B lymphocytes. II. Optimization of activation by anti-immunoglobulin antibody bound to beads: analysis of the role of autocrine effects on B-cell proliferation and of T-cell help in B-cell di

    abstract::We report experiments attempting to optimize the proliferative response of human B cells to rabbit anti-immunoglobulin antibody (RAHIg)-linked beads (anti-Ig beads). By choosing polyacrylamide beads of small size (3 micron) and coupling anti-Ig to them at high concentrations, beads were obtained which were both B-cell...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Boyd AW,Freedman AS,Horowitz JC,Anderson KC,Fisher DC,Rosen KJ,Schlossman SF,Nadler LM

    更新日期:1986-04-15 00:00:00

  • Concurrent enhancement of monocyte immunoregulatory properties and effector functions by recombinant interferon-gamma.

    abstract::Interferon-gamma is a critical factor in the activation of several mononuclear phagocyte effector and immunoregulatory properties. However, it remains uncertain if IFN-gamma is capable of concurrent activation of both functions in the same cell population. Plastic adherent mononuclear cells (80-98% MN by cytochemical ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Tweardy DJ,Fujiwara H,Scillian JJ,Ellner JJ

    更新日期:1986-06-01 00:00:00

  • Impaired anti-tumor cytotoxicity of macrophages from osteopontin-deficient mice.

    abstract::Osteopontin (OPN) expression in tumors is associated with more aggressive tumor growth; however, several studies have suggested that OPN as a host protein can regulate tumor growth as well. OPN is produced by macrophages and T cells, and reportedly modifies macrophage function. Here, we have investigated the effect of...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Bourassa B,Monaghan S,Rittling SR

    更新日期:2004-01-01 00:00:00

  • Differential effect on fresh and cultured T cells of PHA-induced changes in free cytoplasmic calcium: relation to IL-2 receptor expression, IL-2 production, and proliferation.

    abstract::PHA stimulation of both freshly isolated and IL-2-dependent cultured T cells induced a rapid rise in intracytoplasmic calcium concentration. Chelation of extracellular calcium with EGTA resulted in a failure of PHA to induce a rise in intracytoplasmic calcium, resulting in the fresh T cells in an inhibition of IL-2 pr...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Watman NP,Crespo L,Davis B,Poiesz BJ,Zamkoff KW

    更新日期:1988-01-01 00:00:00

  • Enhanced proliferation of murine T cell lines following interaction of poly(Glu60,Phe40) (GPhe) and antigen-presenting accessory cells. I. GPhe-stimulated enhancement of antigen-dependent proliferative responses.

    abstract::Following interaction of the random polymer (Glu60,Phe40)n (GPhe) with antigen-presenting accessory cells (APC), unusual costimulatory activities were noted in several murine T cell systems. When GPhe, in contrast with other random copolymers (GT,GL), was added during "inhibition" and T cell "repertoire" studies as a ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Liu DC,Grun JL,Maurer PH

    更新日期:1990-08-01 00:00:00

  • The generation of oxygen radicals: a positive signal for lymphocyte activation.

    abstract::The induction of ornithine decarboxylase (ODC) activity in lymphocytes is associated with activation and the initiation of cellular proliferation. ODC is also an essential component in tumor promotion. Phorbol myristic acetate (PMA) is a mitogen for lymphocytes, but can also promote tumor formation. Tumor promotion is...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Fidelus RK

    更新日期:1988-04-15 00:00:00

  • Divergent effects of cyclosporine A on interferon- and mitogen-induced Ly-6A antigen suggest autocrine regulation of Ly-6A expression in activated T cells.

    abstract::The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we com...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Dumont FJ

    更新日期:1987-04-01 00:00:00

  • Interleukin-2 induces apoptosis in mouse thymocytes.

    abstract::Interleukins play a role in the process of T-cell development and, like other cytokines, seem able to modulate apoptosis. Interleukin-2 has been reported to inhibit apoptotic cell death of thymocytes induced in vitro by either activation of CD3/TCR complex or treatment with glucocorticoid hormone. We demonstrate here ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Migliorati G,Nicoletti I,Pagliacci MC,D'Adamio L,Riccardi C

    更新日期:1993-01-01 00:00:00

  • Characterization of functional GTP binding proteins in Jurkat T cell mutants lacking either CD3-Ti or CD2 surface receptors.

    abstract::G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T lymphocytes. Given that mature T cells which lack antigen receptors (CDl-Ti) are refractory to stimulation through CD2 or other accessory molecules, T cell rece...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Moingeon P,Jin YJ,Stebbins CC,Lopez PA,Alcover A,Reinherz EL

    更新日期:1990-07-01 00:00:00

  • Effects of vitamin D on macrophages and myeloid-derived suppressor cells (MDSCs) hyperinflammatory response in the lungs of COVID-19 patients.

    abstract::Vitamin D regulates homeostasis, anti-microbial response, and inflammation. The vitamin D receptors are expressed in the macrophages and other immune cells, regulating the transcription of many different genes, including those coding the anti-microbial peptides. One of the most severe complications of the SARS-CoV-2 i...

    journal_title:Cellular immunology

    pub_type: 杂志文章,评审


    authors: Kloc M,Ghobrial RM,Lipińska-Opałka A,Wawrzyniak A,Zdanowski R,Kalicki B,Kubiak JZ

    更新日期:2021-02-01 00:00:00

  • Interleukin-4 enhances anti-IgM stimulation of B cells by improving cell viability and by increasing the sensitivity of B cells to the anti-IgM signal.

    abstract::The lymphokine IL-4 is a potent enhancer of anti-IgM-induced B cell proliferation. Although the mechanism of this enhancement is not known, a commonly held view suggests that IL-4 acts together with anti-IgM as a costimulating factor for the activation of a subpopulation of B cells. To evaluate this hypothesis we exam...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Hodgkin PD,Go NF,Cupp JE,Howard M

    更新日期:1991-04-15 00:00:00

  • Accessory function of human mononuclear phagocytes for lymphocyte responses to the superantigen staphylococcal enterotoxin B.

    abstract::The role of the cytokines IL-1 alpha, IL-1 beta, and IL-6 and the cell adhesion molecules ICAM-1, LFA-1 (alpha and beta), and Mac-1 as accessory molecules for stimulation of T cells by the superantigen staphylococcal enterotoxin B (SEB) was examined. Both blood monocytes and alveolar macrophages were used as accessory...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Salamon FT,Fayen JD,Leonard ML,Finegan CK,Rich EA

    更新日期:1992-05-01 00:00:00

  • Glucocorticoid-induced alterations of monocyte defense mechanisms against Candida albicans.

    abstract::The mechanisms of glucocorticoid-induced immunosuppression of human monocytes for the defense of Candida albicans were examined in this in vitro study. Dexamethasone at pharmacological concentrations (10(-7)-10(-5) M) dose-dependently attenuated growth inhibition of C. albicans by resident monocytes. In interferon-gam...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Heidenreich S,Kubis T,Schmidt M,Fegeler W

    更新日期:1994-09-01 00:00:00

  • Modulation of the locomotory capacity of human large granular lymphocytes.

    abstract::The regulation of the migratory capacity of Percoll-purified large granular lymphocytes (LGL) into nitrocellulose filters was studied in a 2-hr assay with the use of modified Boyden chambers. Compounds that stimulate the natural killer cytotoxic function of LGL, such as interferons (natural beta, recombinant alpha A, ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Polentarutti N,Bottazzi B,Balotta C,Erroi A,Mantovani A

    更新日期:1986-08-01 00:00:00

  • Infection of hematopoietic stem cells by Leishmania infantum increases erythropoiesis and alters the phenotypic and functional profiles of progeny.

    abstract::Immunosuppression is a well-established risk factor for Visceral Leishmaniasis. Post-immunosuppression leishmaniasis is characterized by an increase of parasite burden, hematopoietic disorders and unusual clinical manifestations. Although there are many reports on bone marrow findings in VL, less is known about the re...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Carvalho-Gontijo R,Moreira DR,Resende M,Costa-Silva MF,Peruhype-Magalhães V,Ribeiro CMF,Ribeiro DD,Silvestre R,Cordeiro-da-Silva A,Martins-Filho OA,Teixeira-Carvalho A

    更新日期:2018-04-01 00:00:00

  • A clinical correlation of anti-DNA-AGE autoantibodies in type 2 diabetes mellitus with disease duration.

    abstract::Nonenzymatic glycation of amino groups of DNA bases by reducing sugars can generate advanced glycation end products (AGEs). Cellular formation of AGEs under normal physiology is continuously scanned and removed by efficient system in the cells. However, excess formation and accumulation of AGEs may be cause or consequ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ashraf JM,Arfat MY,Arif Z,Ahmad J,Moinuddin,Alam K

    更新日期:2015-02-01 00:00:00

  • E3 ubiquitin ligase: A potential regulator in fibrosis and systemic sclerosis.

    abstract::Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis in the skin and internal organs. The pathogenesis of SSc is not completely understood until now. Recently, many studies have focused on the role of E3 ubiquitin ligases in organ fibrosis. However, the possible regulatory mechanisms of E3 ubiqu...

    journal_title:Cellular immunology

    pub_type: 杂志文章,评审


    authors: Huang XL,Zhang L,Duan Y,Wang YJ,Zhao JH,Wang J

    更新日期:2016-08-01 00:00:00

  • A proliferation-inducing ligand (APRIL) induced hyper-production of IgA from tonsillar mononuclear cells in patients with IgA nephropathy.

    abstract::IgA nephropathy (IgAN) is a tonsil-related disease. We previously showed that oligodeoxynucleotides with CpG (CpG-ODN) and B-cell activation factor (BAFF) are involved in hyperproduction of IgA from tonsillar mononuclear cells of patients with IgAN (IgAN-TMCs). In this study, we focused on a proliferation-inducing lig...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Takahara M,Nagato T,Nozaki Y,Kumai T,Katada A,Hayashi T,Harabuchi Y

    更新日期:2019-07-01 00:00:00

  • Th17 pathway in recent-onset autoimmune diabetes.

    abstract:AIMS:Evaluate the participation of IL-17 pathway in T1D pathogenesis. T helper 17 cells are potent, highly inflammatory cells that produce interleukin 17A (IL-17A), considered a mediator of various immune disorders. However, their role in Type 1 diabetes (T1D) pathogenesis in humans is not totally elucidated. METHODS:...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Fores JP,Crisostomo LG,Orii NM,Santos AS,Fukui RT,Matioli SR,de Moraes Vasconcelos D,Silva MERD

    更新日期:2018-02-01 00:00:00

  • The role of interleukin-6 in mitogenic T-cell activation: detection of interleukin-2 heteronuclear RNA by polymerase chain reaction.

    abstract::It has been documented that interleukin-6 (IL-6) supports the proliferation of purified, anti-CD3-stimulated murine T cells. We found that stimulation of human peripheral blood mononuclear cells (PBMCs) with anti-CD3 induced a significant accumulation of IL-6 mRNA, indicating that antigen-mediated T-cell activation ma...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Walz G,Stevens C,Zanker B,Melton LB,Clark SC,Suthanthiran M,Strom TB

    更新日期:1991-05-01 00:00:00

  • The induction and augmentation of macrophage tumoricidal responses by platelet-activating factor.

    abstract::Platelet-activating factor (PAF) has previously been shown to stimulate intracellular signal transductional events similar to the macrophage tumoricidal activators interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS). Macrophages stimulated with IFN-gamma and LPS utilize various cytolytic mediators in order to ki...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Howard AD,Erickson KL

    更新日期:1995-08-01 00:00:00

  • Characterization of a low molecular weight suppressor of lymphocyte proliferation from guinea pig L2C leukemia cells.

    abstract::Conditioned medium (CM) from 24-hr culture of guinea pig L2C B lymphoblastic leukemia cells contained an inhibitor(s) of mitogen- and antigen-stimulated proliferation of syngeneic (strain 2 guinea pigs), allogeneic (Hartley guinea pigs), and xenogeneic (Balb/c mouse, NZW rabbit) lymphocytes. The proliferation of sever...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Collins DP,Cook DJ,Ricardo MJ Jr

    更新日期:1987-04-01 00:00:00

  • Cytostatic and cytolytic activities of macrophages regulation by prostaglandins.

    abstract::Murine peritoneal macrophages (M phi), activated in vivo or in vitro, remarkably inhibited the uptake of thymidine by a lens epithelial cell line, while resident M phi, or M phi induced by thioglycollate, exhibited much lower or no cytostatic capacity. The target cells were partially protected from the cytostatic acti...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Mochizuki M,Zigler JS Jr,Russell P,Gery I

    更新日期:1984-01-01 00:00:00

  • In vitro production of antigen-specific T cells from unprimed mice: role of dexamethasone and anti-IL-10 antibodies.

    abstract::We describe here a culture system for studying the development, in vitro, of antigen-specific CD4 T cells from unprimed mice. T cells from young mice are initially exposed to antigen, such as pigeon cytochrome C or keyhole limpet hemocyanin, in the presence of adherent accessory cells and then allowed to proliferate i...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Dozmorov I,Miller RA

    更新日期:1997-06-15 00:00:00

  • Thymus hormones do not induce proliferative ability or cytolytic function in PNA+ cortical thymocytes.

    abstract::A variety of thymus hormone preparations, as well as drugs known to perturb cell differentiation, were tested for their ability to induce nonfunctional cortical thymocytes to become functional precursor cells. Murine cortical thymocytes, defined as the high peanut agglutinin (PNA) binding or as the low H-2K, major [86...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Andrews P,Shortman K,Scollay R,Potworowski EF,Kruisbeek AM,Goldstein G,Trainin N,Bach JF

    更新日期:1985-04-01 00:00:00

  • Characterization of natural killer cells induced in the peritoneal exudates of mice infected with Listeria monocytogenes: a study of their tumor target specificity and their expression of murine differentiation antigens and human NK-associated antigens.

    abstract::That M1/70, a monoclonal anti-murine macrophage antibody, recognizes murine natural killer cells (NK) and that there is an increase in NK following intraperitoneal infection with live Listeria monocytogenes (LM) was previously reported. Here, LM-induced NK cells were further characterized with respect to tumor target ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Holmberg LA,Ault KA

    更新日期:1984-11-01 00:00:00

  • Suppressor cell regulation of encephalitogenic T cell lines: generation of suppressor macrophages with cyclosporin A and myelin basic protein.

    abstract::Chronic relapsing experimental allergic encephalomyelitis (CR-EAE) can be adoptively transferred using myelin basic protein (BP)-specific helper T cell lines, and suppressor cells may be important in recovery from EAE. In order to generate suppressor cells, spleen cells obtained from BP-complete Freund's adjuvant (CFA...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Whitham RH,Vandenbark AA,Bourdette DN,Chou YK,Offner H

    更新日期:1990-04-01 00:00:00

  • T-cell receptor gamma delta diversity and specificity of intestinal intraepithelial lymphocytes: analysis of IEL-derived hybridomas.

    abstract::The phenotype, T-cell antigen receptor (TCR) usage and specificity of intestinal intraepithelial lymphocytes (IEL), and hybridomas derived from IEL have been examined. In young conventionally reared mice, approximately 50% of the IEL express TCR alpha beta and 50% express TCR gamma delta, although there is considerabl...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Sydora BC,Mixter PF,Houlden B,Hershberg R,Levy R,Comay M,Bluestone J,Kronenberg M

    更新日期:1993-12-01 00:00:00

  • Triggering HLA-DR molecules on human peripheral monocytes induces their death.

    abstract::Although engagement of MHC class II molecules on human monocytes triggers various cellular events, their possible role in monocyte death is yet unknown. We demonstrate that ligation of MHC class II on primary monocytes induces a rapid cell death that has all the characteristics of monocyte apoptosis, does not require ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Thibeault A,Zekki H,Mourad W,Charron D,Al-Daccak R

    更新日期:1999-02-25 00:00:00