Abstract:
:Two monoclonal antibodies specific for different rat T-cell subpopulations, the anti-helper-T-cell antibody, W3/25, and the OX8 suppressor cell antibody were used to investigate lectin-stimulated T-lymphocyte differentiation of F-344 rat bone marrow cells in culture. Cytofluorometric analysis of freshly isolated lymphocytes from thymus and spleen revealed that these tissues contained both W3/25- and OX8-positive populations but differed with respect to the number of cells and receptor density distribution. By contrast, bone marrow-derived lymphocytes exhibited negligible W3/25- or OX8-associated fluorescence. However, several days after stimulation of bone marrow lymphocytes with phytohemagglutinin (PHA), cells appeared bearing these markers. Two-parameter histogram analysis of light scatter measurements with cell surface immunofluorescence indicated that this phenomenon represented the appearance of a new population of cells, presumably mature T cells, bearing an increased density of marker. These findings suggest an induction of differentiation of bone marrow T precursor cells by nonthymic factors (PHA) since lymphocytes lacking mature T-cell marker expression developed this characteristic after several days in culture.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Pfeifer RW,Stillman WS,Irons RDdoi
10.1016/0008-8749(83)90160-0subject
Has Abstractpub_date
1983-12-01 00:00:00pages
258-68issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(83)90160-0journal_volume
82pub_type
杂志文章abstract::Activated natural killer T (NKT) cells produce a broad range of cytokines, including IL-4 and IFN-gamma, that determine immunomodulatory functions in various animal models. In this report, we show that a well-known proinflammatory cytokine, IL-17 is also produced by a distinct population of NKT cells upon TCR stimulat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.03.004
更新日期:2008-01-01 00:00:00
abstract::Stroke is the leading cause of disability in the United States. Sex differences, including smaller infarcts in females and greater involvement of immune-mediated inflammation in males may affect the efficacy of immune-modulating interventions. To address these differences, we sought to identify distinct stroke-modifyi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.06.003
更新日期:2017-08-01 00:00:00
abstract::The utility of multiple antigenic peptides (MAPs) for the induction of antibody and cellular immune responses in animal models has been demonstrated for a variety of peptide epitopes involved in human disease. However, little is known about immune responses to MAPs constructed with antigenic tumor epitopes, nor has pe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0310
更新日期:1996-12-15 00:00:00
abstract::The NK-susceptibility of dystrophic mouse myoblast cells was investigated. Spleen cells from 8- to 10-week-old normal (+/+) and dystrophic (dy2J/dy2J) male C57BL/6J mice were fractionated on Percoll density gradients and the cells at each density interface were incubated with either 51Cr-labeled YAC-1 or myoblast cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90003-1
更新日期:1986-06-01 00:00:00
abstract::Transcriptional regulation of gene expression is a key component of orchestrating proper immune cell development and function. One strategy for maintaining these transcriptional programs has been the evolution of transcription factor families with members possessing overlapping functions. Using the germ line deletion ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.02.009
更新日期:2015-05-01 00:00:00
abstract::Cultures of gradient-purified human peripheral blood mononuclear cells (PBMC) have been employed to examine the effects of three bacteria-derived human leukocyte interferon subtypes on certain aspects of in vitro immune responses. The addition of highly purified IFN-alpha 1, -alpha 2, -alpha 2/alpha 1 to PMBC cultures...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90161-2
更新日期:1983-12-01 00:00:00
abstract::Enteric immunization with schistosome ova results in a diminished granulomatous response. This study explored a mechanism by which enteric immunization may decrease granuloma size. Granulomas from livers of acutely infected mice were dissociated and the dispersed cells were depleted of macrophages. As defined by a dir...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90227-9
更新日期:1987-09-01 00:00:00
abstract::Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d present...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90371-x
更新日期:1984-06-01 00:00:00
abstract::This study was conducted on human K562 lymphocytes to investigate the mechanisms implicated in the regulation of the serotonin transport process. The uptake of serotonin in these cells was saturable (Km, 3.37 microM; Vmax, 2.03 nmol/10(6) cells) and Na+ dependent; isoosmotic replacement of Na+ with choline chloride in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0242
更新日期:1996-09-15 00:00:00
abstract::The objective of the present investigation was to evaluate the requirement for increased ornithine decarboxylase (ODC) activity and polyamine biosynthesis in the induction of cytolytic T lymphocytes (CTL). In this regard, we have utilized alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ODC. DFMO tre...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90060-8
更新日期:1987-03-01 00:00:00
abstract::Monocytes and macrophages link the innate and adaptive immune systems and protect the host from the outside world. In inflammatory disorders their activation leads to tissue damage. Galectins have emerged as central regulators of the immune system. However, if they regulate monocyte/macrophage physiology is still unkn...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.003
更新日期:2011-01-01 00:00:00
abstract::We have studied the effects of prostaglandin E2 (PGE2) and cholera toxin, two modulators of adenylyl cyclase, and 8-bromo cAMP (8-BrcAMP) on various parameters of lymphocyte activation using the human T cell line Jurkat. Our results show that PGE2 and cholera toxin inhibit, in a dose-related manner, the phytohemagglut...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90035-p
更新日期:1990-07-01 00:00:00
abstract::We tested the contribution of four staphylococcal components - PSM-α, PSM-β, δ-toxin, and PVL - in triggering macrophage secretion of tumor necrosis factor (TNF) and interleukins 6 (IL-6) and 12 (IL-12) by two prominent, circulating strains of community-associated, methicillin-resistant Staphylococcus aureus (CA-MRSA)...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.03.009
更新日期:2011-01-01 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a curable strategy for the treatment of hematological malignancies and nonmalignant diseases. However, graft-versus-host disease (GVHD) and relapse are still two major causes of morbidity and mortality after allo-HSCT, and both restrict the improvement ...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2019.103991
更新日期:2019-12-01 00:00:00
abstract::The Lyt phenotype of cytotoxic T cells generated in the primary H-2 response was investigated kinetically. The cytotoxicity generated in the early stage of culture was abolished by treatment with alpha Lyt-1,2,3, and complement (C), whereas that generated in the late stage was only partially eliminated by alpha Lyt-1,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90126-6
更新日期:1984-08-01 00:00:00
abstract::Previous studies have shown that the anti-CD4 mAb W3/25 strongly enhances T cell APC (T-APC) activity. In this study, single positive CD4+ and double negative (DN) (CD4-CD8-) T-helper cells specific for the 55-69 or 72-86 sequence of guinea pig (GP) myelin basic protein (GPMBP) were used to study CD4 regulation of T-A...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1299
更新日期:1998-06-15 00:00:00
abstract::CD48, the murine homolog of human CD58, binds to CD2 in rats and mice. Whereas inhibition of CD2 signaling leads to profound immunosuppression, no information is available on CD48-targeted therapy in the rat. We could show that anti-CD2 treatment (OX34) efficiently inhibited TCR-driven as well as CD2-mediated prolifer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1204
更新日期:1997-11-25 00:00:00
abstract::Autoimmune regulator (Aire) is a transcriptional activator that regulates the ectopic expression of many tissue-restricted antigens in medullary thymic epithelial cells, and that has an important role in the negative selection of autoreactive T cells. However, the roles of Aire expression in peripheral lymphoid tissue...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.05.002
更新日期:2011-01-01 00:00:00
abstract::The membrane phenotype of T cells involved in delayed hypersensitivity (DH), protective immunity, and suppression of delayed hypersensitivity to herpes simplex virus (HSV) has been determined. T cells from immune lymph nodes transferring DH and antiviral immunity to normal recipients were characterized as Lyt 1+2-. Th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90332-5
更新日期:1983-02-01 00:00:00
abstract::It has been documented that interleukin-6 (IL-6) supports the proliferation of purified, anti-CD3-stimulated murine T cells. We found that stimulation of human peripheral blood mononuclear cells (PBMCs) with anti-CD3 induced a significant accumulation of IL-6 mRNA, indicating that antigen-mediated T-cell activation ma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90322-3
更新日期:1991-05-01 00:00:00
abstract::Mitogenic stimulation of sublines of the human histiocytic lymphoma, U937, results in the production of an inhibitor of lymphocyte proliferation (IDS-U937). The relationship of IDS-U937 to alpha- and gamma-interferon was examined by several techniques: (1) the antiviral activity of the IDS preparations was determined,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90390-3
更新日期:1984-07-01 00:00:00
abstract::Transforming growth factor beta1 (TGFbeta1) is a potent negative immunoregulatory molecule. We have previously shown that the autoimmune-mediated weaning-age lethality of Tgfb1-/- mice is reversed upon genetic combination with Scid or Rag null alleles. Here, we show that elimination of T but not B cells is sufficient ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.02.004
更新日期:2004-11-01 00:00:00
abstract::The glycobiology of the immune response is a topic that has garnered increased attention due to a number of key discoveries surrounding IgG function, the specificity of some broadly neutralizing anti-HIV antibodies, cancer immunoregulation by galectin molecules and others. This review is the opening article in a Speci...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2018.04.010
更新日期:2018-11-01 00:00:00
abstract::We have established murine T-cell clones which respond to allotypic and species-specific determinants found on chicken erythrocytes (cRBC). Their relative antigen specificities were determined by assessing lymphokine production and proliferation in response to syngeneic spleen cells and cRBC obtained from chickens hom...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90406-5
更新日期:1986-02-01 00:00:00
abstract::Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a chronically intestinal autoimmune disease, the pathological mechanisms of which are not very clear. Wild type p-53 induced phosphatase 1 (Wip1), a serine/threonine protein phosphatase, has been reported to negatively reg...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.07.012
更新日期:2016-12-01 00:00:00
abstract::The hematopoietically restricted member of the NF-kappaB/Rel family, c-Rel, is essential for B cell survival and proliferation. Here we demonstrate that the production of the interleukins 6, 10, and 15 (IL-6, IL-10, and IL-15) are diminished in c-Rel(-/-) B lymphocytes. In a manner similar to that seen in IL-6(-/-) B ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00513-0
更新日期:2002-05-01 00:00:00
abstract::In this study, skin-infiltrating cells were prepared from guinea pig delayed-type hypersensitivity reaction sites, and their phenotypic properties and cytokine production were examined. Our cell preparations contained around 45% nonspecific esterase-positive cells and a few skin-derived cells. Skin-infiltrating cells ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1139
更新日期:1995-08-01 00:00:00
abstract::In the present study, we characterized in monocytes the rise in [Ca(2+)](i) evoked by monoclonal antibodies (mAbs) to aminopeptidase N (APN)/CD13, showing a two-phase calcium increase with a small-belled [Ca(2+)](i) rise due to the release of calcium from intracellular stores and a more sustained plateau due to the in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1629
更新日期:2000-04-10 00:00:00
abstract::We have investigated the ability of interleukin-10 (IL-10) to modulate murine intrathymic T cell differentiation using a fetal thymic organ culture (FTOC) model. Addition of as little as 11 ng of recombinant murine IL-10 (mIL-10) per day produced a significant increase in the proportion and number of gamma delta-TCR+ ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1105
更新日期:1994-04-15 00:00:00
abstract::The transfer of B cells from mice immunized with Type III pneumococcal polysaccharide (SSS-III) results in the activation of suppressor and amplifier T cells that control the magnitude of the antibody response in recipient mice, immunized subsequently with SSS-III. Prior treatment of transferred B cells with an excess...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90368-5
更新日期:1985-12-01 00:00:00