Abstract:
:CD48, the murine homolog of human CD58, binds to CD2 in rats and mice. Whereas inhibition of CD2 signaling leads to profound immunosuppression, no information is available on CD48-targeted therapy in the rat. We could show that anti-CD2 treatment (OX34) efficiently inhibited TCR-driven as well as CD2-mediated proliferation, whereas blocking of ligand binding (OX45) remained completely uneffective. Inhibition of allogeneic MLR by OX45 turned out to be due to induction of unspecific suppressive mechanisms. In vivo, OX45 failed to prolong rat heart allograft survival in contrast to that seen with OX34. Grafts were rejected despite persistent and complete downmodulation of CD48 on lymphocytes without any cell depleting effect, rendering receptor/ligand interactions physically impossible. Combined application of CD2 and CD48 mAb did not enhance immunosuppression induced by CD2 mAb alone. Provided that there is no alternative CD2 ligand in the rat, we conclude that CD2-directed immunotherapy is mediated by suppressive events induced by modulation of the CD2 receptor ("negative signaling") rather than by mere disruption of the CD2-CD48 interaction.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Sido B,Otto G,Zimmermann R,Müller P,Meuer SC,Dengler TJdoi
10.1006/cimm.1997.1204subject
Has Abstractpub_date
1997-11-25 00:00:00pages
57-67issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(97)91204-1journal_volume
182pub_type
杂志文章abstract::Peripheral blood leukocytes (PBL), nonadherent lymphocytes, and adherent monocytes separated from freshly isolated blood of 15 dogs were analyzed for their ability to mediate antibody-dependent cell-mediated cytotoxicity (ADCC) in combination with murine anti-tumor monoclonal antibodies (MAbs). Canine monocytes isolat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90194-3
更新日期:1988-09-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis (MS), and is induced by immunization with disease-causative self-antigens such as myelin oligodendrocyte glycoprotein (MOG). We have previously reported that transplantation of MOG expressing thymic epithelial progenitors (TEPs) ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.10.007
更新日期:2017-12-01 00:00:00
abstract::Basic Immunology has had only two significant public priority disputes. The first began in the late 1650s and concerned the recognition of the peripheral network of vessels which collects lymph throughout the body. The publication of this major anatomical discovery prompted a priority feud discussed in a previous pape...
journal_title:Cellular immunology
pub_type: 历史文章,杂志文章
doi:10.1016/j.cellimm.2007.02.015
更新日期:2007-01-01 00:00:00
abstract::The primary objective of this study was to determine whether the n-3 PUFA-mediated changes in host response to a Listeria monocytogenes infection (e.g., cytokine production and bacterial clearance) were dependent upon neutrophils. Balb/c mice were fed one of two semi-purified diets that contained either 0 or 41 g of n...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.07.003
更新日期:2005-05-01 00:00:00
abstract::Vaccination can elicit CD8(+) cytotoxic T lymphocytes (CTL) that recognize peptides presented by class I MHC molecules. Relatively little is known, however, about the genetic factors that shape the repertoire of T cell clonotypes responding to any given epitope. We report here that H-2(b) mice immunized with a plasmid...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1338
更新日期:1998-08-25 00:00:00
abstract::Histidine-proline-rich glycoprotein (HPRG) is a plasma protein which binds to a specific receptor on T-lymphocytes and represses T-cell activation and proliferation. In the presence of Concanavalin A (Con A), HPRG causes human T-lymphoblastic MOLT-3 cells and a fraction of normal human peripheral blood lymphocytes to ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1311
更新日期:1993-12-01 00:00:00
abstract::We compared macrophage binding and killing of F5b cells to the binding and killing of P815 mastocytoma cells and to several other nontransformed and transformed cell lines. Formalin fixation of elicited or activated macrophages did not affect binding of F5b or 3T3 cells but did abrogate binding of P815 cells. However,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90190-m
更新日期:1991-03-01 00:00:00
abstract::The quantitative organ distribution and tissue microenvironment positioning of radioisotopically labeled cloned T cells were characterized. Intravenous (iv) injection of 51chromium (51Cr)-labeled, long-term cultured cloned T-helper cells and cells from several cloned cytolytic T-lymphocyte lines (CTLL) resulted in poo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90349-0
更新日期:1983-02-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90389-7
更新日期:1984-07-01 00:00:00
abstract::We previously showed that DT-5461a, a lipid A analog, has antitumor activity and relatively low toxicity in animals. To clarify its immunostimulatory effect we have studied the cytokine inducibility of DT-5461a and the mechanism involved in the cytokine expression in macrophages. While the activity is lower than that ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1100
更新日期:1994-04-15 00:00:00
abstract::Autoimmune regulator (Aire) is a transcriptional activator that regulates the ectopic expression of many tissue-restricted antigens in medullary thymic epithelial cells, and that has an important role in the negative selection of autoreactive T cells. However, the roles of Aire expression in peripheral lymphoid tissue...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.05.002
更新日期:2011-01-01 00:00:00
abstract::The murine T cell hybridoma line, MBI-1.15, secretes a 17-kDa protein which decreases binding activity of the CD23 molecule for its natural ligand, IgE. This protein, denoted epsilon receptor-modulating protein (epsilon RMP), was previously characterized and shown to be a novel serine protease. The present studies sho...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1036
更新日期:1994-02-01 00:00:00
abstract::The oral administration of myelin basic protein (MBP) to Lewis rats prior to an encephalitogenic challenge resulted in total inhibition or a significant delay in the onset of experimental autoimmune encephalomyelitis (EAE). In vitro lymphocyte proliferative responses to MBP were significantly decreased in MBP-fed rats...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90305-x
更新日期:1988-04-01 00:00:00
abstract::Heterotrimeric G-proteins control diverse biological processes by conveying signals from seven-transmembrane receptors to intracellular effectors. Although their signaling roles were originally ascribed to their GTP-bound alpha-subunits, more recent evidence points to the equally active roles played by their betagamma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.06.008
更新日期:2006-04-01 00:00:00
abstract::Transforming growth factor beta1 (TGFbeta1) is a potent negative immunoregulatory molecule. We have previously shown that the autoimmune-mediated weaning-age lethality of Tgfb1-/- mice is reversed upon genetic combination with Scid or Rag null alleles. Here, we show that elimination of T but not B cells is sufficient ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.02.004
更新日期:2004-11-01 00:00:00
abstract::Microglia are important immunoregulatory cells within the central nervous system (CNS). Viral infection of primary microglia and splenic macrophage clones revealed that both exhibited a heterogeneous, but relatively low, sensitivity to cytolysis mediated by CD8(+) cytotoxic T lymphocytes (CTL). The majority of clones ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1581
更新日期:1999-11-25 00:00:00
abstract::The development of B cell tolerance is believed to involve negative signaling to B cells derived from the binding of antigen to the B cell surface immunoglobulin. B cell lines that receive negative signals may provide useful models for studying the mechanisms of B cell tolerance. We have established a human B lymphoma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1314
更新日期:1994-12-01 00:00:00
abstract::The role of protein synthesis during the activation of macrophages (M phi) by lymphokines (LK) was studied. Peritoneal murine macrophages elicited by proteose-peptone (pM phi) were activated with LK (supernatants from normal mouse spleen cells pulsed with concanavalin A) and tested for cytotoxicity in an 18 hr assay a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90273-9
更新日期:1984-04-15 00:00:00
abstract::The pathogenic antibody response to acetylcholine receptor (AChR) in experimental autoimmune myasthenia gravis (EAMG) is T cell dependent. Therefore, it should be possible to design specific immunotherapeutic approaches to treat EAMG (and human MG) by interfering with AChR-specific helper T cells. Productive T cell ac...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.0012
更新日期:1995-11-01 00:00:00
abstract::The molecular mechanism responsible for the age-dependent decline of natural killer (NK) cell response was examined. The responses of NK cells to poly(I:C), Con A, and LPS were compared between the young and the aged animals. We observed that both basal and induced NK cell activity declined with age. To further determ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0271
更新日期:1996-11-01 00:00:00
abstract::The proliferative responses of human peripheral blood mononuclear cells (PBL) and thymocytes to OKT3 monoclonal antibody have been investigated. The PBL response to OKT3 was maximal after 72 hr while that of thymocytes was inappreciable at all times measured. Unlike phytohemagglutinin, OKT3 was unable to elicit the mi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90105-9
更新日期:1984-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1605
更新日期:2000-02-01 00:00:00
abstract::We have investigated the ability of interleukin-10 (IL-10) to modulate murine intrathymic T cell differentiation using a fetal thymic organ culture (FTOC) model. Addition of as little as 11 ng of recombinant murine IL-10 (mIL-10) per day produced a significant increase in the proportion and number of gamma delta-TCR+ ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1105
更新日期:1994-04-15 00:00:00
abstract::C3H/HeJ mice are homozygous for the Lpsd allele and, as a consequence, are hyporesponsive to all of the biological effects of bacterial lipopolysaccharide (LPS) that have been studied. These mice die in the early phase of infection when inoculated with virulent Salmonella. This susceptibility is also regulated by the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90326-6
更新日期:1986-10-01 00:00:00
abstract::Transformed murine fetal thymocyte cell lines were derived by incubating fetal thymic organ cultures with a v-myc/v-raf-containing retroviral construct in order to model developmental stages within the early triple negative (CD3-CD4-CD8-) thymocyte population. The resulting 10 cell lines had a lymphoid morphology, wer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0170
更新日期:1996-07-10 00:00:00
abstract::Short-term culture of murine lymphocytes in interleukin 2 (IL-2), in the absence of any priming antigen, has been shown to result in the differentiation of an activated killer cell population capable of potent cytotoxic activity against tumor cells. The progenitor and lineage of these lymphokine activated killer cells...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90089-4
更新日期:1986-12-01 00:00:00
abstract::Nonenzymatic glycation of amino groups of DNA bases by reducing sugars can generate advanced glycation end products (AGEs). Cellular formation of AGEs under normal physiology is continuously scanned and removed by efficient system in the cells. However, excess formation and accumulation of AGEs may be cause or consequ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2014.12.007
更新日期:2015-02-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90324-3
更新日期:1988-07-01 00:00:00
abstract::Two interleukin-2 (IL-2)-dependent cytotoxic T-cell clones were obtained by limiting dilution from a lymphocyte culture stimulated in vitro with the autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) in the presence of fetal calf serum (FCS). Both clones uniformly had a T3+, T4+, Dr+ phenotype an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90304-7
更新日期:1986-04-01 00:00:00
abstract::Since the days of Medawar, the goal of therapeutic tolerogenesis has been a "Holy Grail" for immunologists. While knowledge of cellular and molecular mechanisms of this process has been increasing at an exponential rate, clinical progress has been minimal. To provide a mechanistic background of tolerogenesis, we overv...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2010.04.002
更新日期:2010-01-01 00:00:00