当前位置： SCI文献检索 > CELLULAR IMMUNOLOGY期刊下所有文献 > Development and characterization of v-myc/v-raf-transformed murine fetal thymocyte cell lines.

Development and characterization of v-myc/v-raf-transformed murine fetal thymocyte cell lines.

Abstract：

:Transformed murine fetal thymocyte cell lines were derived by incubating fetal thymic organ cultures with a v-myc/v-raf-containing retroviral construct in order to model developmental stages within the early triple negative (CD3-CD4-CD8-) thymocyte population. The resulting 10 cell lines had a lymphoid morphology, were all CD44+, CD90+, and were triple negative by surface antigen analysis. The cell lines, however, were distinguishable by differences in the expression of T cell-associated and T cell-specific genes. The CD3 genes were observed to be discoordinately expressed, in that CD3 gamma chain gene expression was detected in 2 cell lines in the absence of CD3 delta and epsilon expression. Expression of the CD3 gamma chain gene was observed in cell lines without the expression of other T cell-specific genes or T cell receptor rearrangement and may be one of the earliest T cell-specific genes to be expressed. The transcription factor Ikaros was expressed in all 10 cell lines, whereas the transcription factor TCF1 alpha was expressed only in the 2 most differentiated lines. In 8 cell lines, expression of partial TCR beta and/or TCR alpha transcripts was observed by Northern blot. In several lines, expression of rearranged TCR alpha transcripts in the absence of TCR beta transcripts was demonstrated; however, TCR beta DJ rearrangements were observed by Southern blot in all but 1 of these cell lines. Thus, these cell lines, ordered based on the general pattern of additive gene expression observed, may reflect various stages of triple-negative thymocyte differentiation and provide an in vitro mechanism to elucidate some of the molecular events involved in early thymocyte development.

journal_name

Cell Immunol

journal_title

Cellular immunology

authors

Taubenberger JK,Reid AH,Izon D,Boehme SA

doi

10.1006/cimm.1996.0170

subject

Has Abstract

pub_date

1996-07-10 00:00:00

pages

41-7

issue

1

eissn

0008-8749

issn

1090-2163

pii

S0008-8749(96)90170-7

journal_volume

171

pub_type

杂志文章

相关文献

CELLULAR IMMUNOLOGY文献大全
  • Studies on the mechanism of suppression of experimental allergic encephalomyelitis induced by myelin basic protein-cell conjugates.

    abstract：:The mechanism of suppression of experimental allergic encephalomyelitis (EAE) induced in Lewis rats by pretreatment with myelin basic protein (MBP) coupled to syngeneic spleen leukocytes (SL) was examined. Studies on the kinetics of the tolerance induction showed that pretreatment with MBP-SL suppressed EAE if given 7...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90159-x

    authors： McKenna RM,Carter BG,Sehon AH

    更新日期：1984-10-15 00:00:00

  • NK and LAK activities from human marrow progenitors. I. The effects of interleukin-2 and interleukin-1.

    abstract：:We have investigated the role of interleukin-2 (IL2) as a differentiation factor for human marrow-derived NK cell progenitors and have assessed the effects of interleukin-1 (IL1) on this activity. The effects of these cytokines on early NK cell precursors was determined by testing marrow which had been depleted of mat...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(90)90313-g

    authors： Keever CA,Pekle K,Gazzola MV,Collins NH,Gillio A

    更新日期：1990-03-01 00:00:00

  • Characterization of natural killer cells induced in the peritoneal exudates of mice infected with Listeria monocytogenes: a study of their tumor target specificity and their expression of murine differentiation antigens and human NK-associated antigens.

    abstract：:That M1/70, a monoclonal anti-murine macrophage antibody, recognizes murine natural killer cells (NK) and that there is an increase in NK following intraperitoneal infection with live Listeria monocytogenes (LM) was previously reported. Here, LM-induced NK cells were further characterized with respect to tumor target ...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90206-5

    authors： Holmberg LA,Ault KA

    更新日期：1984-11-01 00:00:00

  • Evaluation of metabolic and immunological changes in streptozotocin-nicotinamide induced diabetic rats.

    abstract：:Type 2 diabetes is a chronic disease with growing public health concern globally. Finding remedies to assist this health issue requires recruiting appropriate animal model for experimental studies. This study was designated to evaluate metabolic and immunologic changes in streptozotocin-nicotinamide induced diabetic r...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2014.04.004

    authors： Mojani MS,Sarmadi VH,Vellasamy S,Sandrasaigaran P,Rahmat A,Peng LS,Ramasamy R

    更新日期：2014-05-01 00:00:00

  • Adhesion of lymphoid cells to fibroblasts in tissue culture.

    abstract：:In this study we have examined the cellular and molecular specificity of lymphocyte interaction with fibroblasts. Using mitogen-activated T-cells, we found that attachment to fibroblasts was highly sensitive to protease treatment, and to an antibody raised against the purified lymphocyte plasma membrane, but it was no...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(89)90146-9

    authors： Abraham D,Bou-Gharios G,Muir H,Olsen I

    更新日期：1989-08-01 00:00:00

  • An autoreactive T hybridoma expressing nonspecific helper activity.

    abstract：:Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d present...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90371-x

    authors： Folks TM,Sell KW

    更新日期：1984-06-01 00:00:00

  • The regulatory role of Hyper-IL-6 in the differentiation of myeloid and erythroid progenitors derived from human cord blood.

    abstract：:This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34(+)CD38(-...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2006.07.008

    authors： Yu Z,Liu W,Liu D,Fan L

    更新日期：2006-05-01 00:00:00

  • Uveitogenicity is associated with a Th1-like lymphokine profile: cytokine-dependent modulation of early and committed effector T cells in experimental autoimmune uveitis.

    abstract：:This study addresses the nature of the pathogenic effector T cell in experimental autoimmune uveoretinitis and the effect of different cytokines on these cells in vitro. Lymph node cells of B10.RIII mice immunized with the uveitogenic peptide 161-180 of interphotoreceptor retinoid binding protein were cultured with th...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1997.1121

    authors： Xu H,Rizzo LV,Silver PB,Caspi RR

    更新日期：1997-05-25 00:00:00

  • Paradoxical production of mouse thymocyte activating factor by ouabain-treated human mononuclear cells.

    abstract：:At concentrations as low as 10(-7) M, the cardiotonic glycosteroid ouabain, a specific inhibitor of the membrane Na+, K+-ATPase, is known to inhibit in vitro human lymphocyte proliferation produced in mixed lymphocyte cultures or induced by various stimulating agents (PHA, Con A, PWM, soluble antigens), while mouse ly...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90314-9

    authors： Dornand J,Favero J,Bonnafous JC,Mani JC

    更新日期：1984-02-01 00:00:00

  • In vivo localization of cloned IL-2-dependent T cells.

    abstract：:The quantitative organ distribution and tissue microenvironment positioning of radioisotopically labeled cloned T cells were characterized. Intravenous (iv) injection of 51chromium (51Cr)-labeled, long-term cultured cloned T-helper cells and cells from several cloned cytolytic T-lymphocyte lines (CTLL) resulted in poo...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(83)90349-0

    authors： Carroll AM,Palladino MA,Oettgen H,De Sousa M

    更新日期：1983-02-15 00:00:00

  • Induction of linked suppression in addition to the donor H-2 class I-specific unresponsiveness in recipient T cells by transfusing class I plus class II-disparate, but not class I alone-disparate, bone marrow cells.

    abstract：:This study was undertaken to determine whether bone marrow (BM) cells contain a cell population with the capacity to induce an unresponsiveness of T cells specific to the BM self-H-2 class I antigens in vivo, i.e., veto cell population. Recombinant or congenic mice were infused intravenously with H-2-incompatible BM c...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(90)90024-l

    authors： Muraoka S

    更新日期：1990-06-01 00:00:00

  • Stimulator requirements for primed alloreactive T cells: macrophages and dendritic cells activate T cells across all genetic disparities.

    abstract：:The cellular requirements for stimulating primed alloreactive T cells have been investigated. In vitro-primed secondary alloreactive cells, long-term lines, and Ly 1+2- noncytolytic clones which reacted with allo-H-2K, D, or Mls (M locus) antigens were tested. The data indicated that a specialized antigen-presenting c...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(85)90032-2

    authors： Sunshine GH,Mitchell TJ,Czitrom AA,Edwards S,Glasebrook AL,Kelso A,MacDonald HR

    更新日期：1985-03-01 00:00:00

  • Immunological properties of umbilical cord blood-derived mesenchymal stromal cells.

    abstract：:Mesenchymal stromal cells (MSCs) are promising candidates for developing cell therapies for intractable diseases. To assess the feasibility of transplantation with human umbilical cord blood (hUCB)-derived MSCs, we analyzed the ability of these cells to function as alloantigen-presenting cells (APC) in vitro. hUCB-MSC...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2008.04.003

    authors： Oh W,Kim DS,Yang YS,Lee JK

    更新日期：2008-02-01 00:00:00

  • Effects of ultraviolet-B irradiation on human LAK and NK cytotoxic activity.

    abstract：:In vitro ultraviolet-B (UVB) irradiation of murine and rodent bone marrow cells prevents GVHD without compromising engraftment while inducing tolerance to donor-type allografts. In anticipation of clinical trials of UVB-modified bone marrow grafts, we studied the in vitro effects of UVB irradiation (50-300 J/m2) on hu...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1995.1202

    authors： Yaron I,Zakheim AR,Oluwole SF,Hardy MA

    更新日期：1995-10-15 00:00:00

  • MFG-E8 inhibits Aβ-induced microglial production of cathelicidin-related antimicrobial peptide: A suitable target against Alzheimer's disease.

    abstract：:Neuroinflammation plays a pivotal role in the incidence and progression of Alzheimer's disease (AD). Cathelicidin-related antimicrobial peptide (CRAMP) is critically involved in the innate neuronal responses of chronic neuroinflammation in AD and thus plays a key role in the disease. Here, we show that Aβ42 induced mi...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2018.05.008

    authors： Xu X,Cai X,Zhu Y,He W,Wu Q,Shi X,Fang Y,Pei Z

    更新日期：2018-09-01 00:00:00

  • Disruption of membrane cholesterol stimulates MyD88-dependent NF-kappaB activation in immature B cells.

    abstract：:Agents that extract or sequester membrane cholesterol stimulate IkappaB degradation and lead to NF-kappaB activation in a subset of B cells. Although the extraction of cholesterol by methyl-beta-cyclodextrin is the most potent stimulus of NF-kappaB, other agents that sequester cholesterol have similar effects. B cells...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2004.06.004

    authors： Flemming JA,Perkins KH,Luus L,Ferguson AR,Corley RB

    更新日期：2004-05-01 00:00:00

  • Murine T-cell clones specific for chicken erythrocyte alloantigens.

    abstract：:We have established murine T-cell clones which respond to allotypic and species-specific determinants found on chicken erythrocytes (cRBC). Their relative antigen specificities were determined by assessing lymphokine production and proliferation in response to syngeneic spleen cells and cRBC obtained from chickens hom...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(86)90406-5

    authors： Giedlin MA,Longenecker BM,Mosmann TR

    更新日期：1986-02-01 00:00:00

  • NFκB and TGFβ contribute to the expression of PTPN3 in activated human lymphocytes.

    abstract：:We previously reported that protein tyrosine phosphatase non-receptor type 3 (PTPN3), which is upregulated in activated lymphocytes, acts as an immune checkpoint. However, the mechanism by which PTPN3 expression is enhanced in activated lymphocytes is unknown. In this study, we analyzed the mechanism of PTPN3 expressi...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2020.104237

    authors： Nakayama K,Onishi H,Fujimura A,Imaizumi A,Kawamoto M,Oyama Y,Ichimiya S,Koga S,Fujimoto Y,Nakashima K,Nakamura M

    更新日期：2020-12-01 00:00:00

  • Studies of the in vitro activation and differentiation of human B lymphocytes. II. Optimization of activation by anti-immunoglobulin antibody bound to beads: analysis of the role of autocrine effects on B-cell proliferation and of T-cell help in B-cell di

    abstract：:We report experiments attempting to optimize the proliferative response of human B cells to rabbit anti-immunoglobulin antibody (RAHIg)-linked beads (anti-Ig beads). By choosing polyacrylamide beads of small size (3 micron) and coupling anti-Ig to them at high concentrations, beads were obtained which were both B-cell...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(86)90231-5

    authors： Boyd AW,Freedman AS,Horowitz JC,Anderson KC,Fisher DC,Rosen KJ,Schlossman SF,Nadler LM

    更新日期：1986-04-15 00:00:00

  • Tumor necrosis factor (TNF alpha) regulates intestinal mucus production during salmonellosis.

    abstract：:Mucus production by goblet cells in the gastrointestinal tract following Salmonella typhimurium infection using a ligated ileal loop model in mice was investigated. Assessment of the morphology of the loop tissue after Salmonella challenge revealed generalized tissue inflammation, characterized by edema and an inflamm...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1993.1243

    authors： Arnold JW,Klimpel GR,Niesel DW

    更新日期：1993-10-15 00:00:00

  • Burn injury suppresses human dermal dendritic cell and Langerhans cell function.

    abstract：:Human skin contains epidermal Langerhans cells (LCs) and dermal dendritic cells (DCs) that are key players in induction of adaptive immunity upon infection. After major burn injury, suppressed adaptive immunity has been observed in patients. Here we demonstrate that burn injury affects adaptive immunity by altering bo...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2011.01.007

    authors： van den Berg LM,de Jong MA,Witte Ld,Ulrich MM,Geijtenbeek TB

    更新日期：2011-01-01 00:00:00

  • Interleukin-10 enhances gamma delta T cell development in the murine fetal thymus.

    abstract：:We have investigated the ability of interleukin-10 (IL-10) to modulate murine intrathymic T cell differentiation using a fetal thymic organ culture (FTOC) model. Addition of as little as 11 ng of recombinant murine IL-10 (mIL-10) per day produced a significant increase in the proportion and number of gamma delta-TCR+ ...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1994.1105

    authors： Fine JS,Macosko HD,Grace MJ,Narula SK

    更新日期：1994-04-15 00:00:00

  • Expression of the pim-1 protooncogene: differential inducibility between alpha/beta- and gamma/delta-T cells and B cells.

    abstract：:The pim-1 gene encodes a serine/threonine protein kinase with expression restricted primarily to cells of hematopoietic lineage and is thought to play a role in the signal transduction events associated with lymphocyte activation. A rapid increase in pim-1 mRNA levels was found after stimulation of normal unseparated ...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1995.1059

    authors： Wingett D,Stone D,Davis WC,Magnuson NS

    更新日期：1995-04-15 00:00:00

  • Specific immune responses but not basal functions of B and T cells are impaired in aged mice.

    abstract：:Senescence is characterized by several alterations in the immune system. Such modifications can be found in lymphoid organs as well as in the cellular components of the immune system. Several reports have suggested that immune dysfunction can affect both T and B cells, but T cells have been shown to be more susceptibl...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2009.01.010

    authors： Speziali E,Santiago AF,Fernandes RM,Vaz NM,Menezes JS,Faria AM

    更新日期：2009-01-01 00:00:00

  • Proliferative response of murine B-cell leukemia (BCL1) to poly(L-lysine).

    abstract：:Peripheral blood lymphocytes (PBL) isolated from BALB/c mice bearing a B-cell leukemia (BCL1) showed a marked proliferative response upon two days culturing with poly(L-lysine) (PLL) of various molecular weights. An inverse relationship was noted between the molecular weight of the PLL and the dose required for optima...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(85)90203-5

    authors： Morecki S,Flechner Y,Slavin S

    更新日期：1985-02-01 00:00:00

  • Interferon-gamma receptor polymorphisms determine strain differences in accessibility of activated lymphocyte NK-triggering antigens to recognition by self-reactive NK cells.

    abstract：:The "NK-triggering-antigen regulator" (Nktar) gene is a locus identified in the C57BL/6 genome which regulates the ability of unlabeled activated Con A blasts to compete for recognition of labeled syngeneic Con A blasts by BALB/c NK cells. Linkage analysis on Con A blasts from (BALB/c x CByB6F1) N2 backcross progeny f...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1999.1624

    authors： Chou SR,Brownell A,Ko MS,Kaplan J

    更新日期：2000-03-15 00:00:00

  • Prepared and screened a modified TNF-alpha molecule as TNF-alpha autovaccine to treat LPS induced endotoxic shock and TNF-alpha induced cachexia in mouse.

    abstract：:Overexpression of TNF-alpha in the body is critically involved in many diseases. A strategy to construct TNF-alpha autovaccine by introducing a T cell helper epitope to the protein has been developed and may be an alternative because it is cheaper and highly efficient. However, the induction of high level anti-TNF-alp...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2007.05.005

    authors： Wan Y,Xue X,Li M,Zhang X,Qin X,Zhang C,You Y,Wang W,Jiang C,Wu S,Liu Y,Zhu W,Ran Y,Zhang Z,Han W,Zhang Y

    更新日期：2007-04-01 00:00:00

  • DNA array and biological characterization of the impact of the maturation status of mouse dendritic cells on their phenotype and antitumor vaccination efficacy.

    abstract：:We systematically investigated the impact of the relative maturation levels of dendritic cells (DCs) on their cell surface phenotype, expression of cytokines and chemokines/chemokine receptors (by DNA array and RNase protection analyses), biological activities, and abilities to induce tumor immunity. Mature DCs expres...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.2001.1883

    authors： Chen Z,Dehm S,Bonham K,Kamencic H,Juurlink B,Zhang X,Gordon JR,Xiang J

    更新日期：2001-11-25 00:00:00

  • Inhibitors of prostaglandin synthesis block the induction of staphylococcal enterotoxin B-activated T-suppressor cells.

    abstract：:A variety of arachidonic acid metabolites possess the ability to modulate immune cell function. Various inhibitors of arachidonic acid metabolism were compared with regard to their effects on T-suppressor (Ts) cell function. Using staphylococcal enterotoxin B (SEB) to activate Lyt-2+ Ts cells, it was shown that indome...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(83)90211-3

    authors： Donnelly RP,Rogers TJ

    更新日期：1983-10-01 00:00:00

  • Anti-inflammatory properties of estrogen. I. In vivo suppression of leukocyte production in bone marrow and redistribution of peripheral blood neutrophils.

    abstract：:We have recently demonstrated that estrogen has differential immunomodulatory properties in mice because it stimulates antibody responses but inhibits T cell-mediated inflammation. In the present study we have examined the influence of estrogen on T cell-independent inflammatory responses. A local inflammation was ind...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(92)90269-u

    authors： Josefsson E,Tarkowski A,Carlsten H

    更新日期：1992-06-01 00:00:00