Abstract:
:We have investigated the role of interleukin-2 (IL2) as a differentiation factor for human marrow-derived NK cell progenitors and have assessed the effects of interleukin-1 (IL1) on this activity. The effects of these cytokines on early NK cell precursors was determined by testing marrow which had been depleted of mature cells and of CD2+ cells by treatment with soybean agglutinin and sheep erythrocytes (SBA-E-BM). The cytolytic activities of the SBA-E-BM were tested in 51Cr release assays following 7-8 days of liquid culture. K562 targets were used to assess NK activity and NK-resistant Daudi targets were used to measure lymphokine-activated killer (LAK) cell activity. Neither NK nor LAK activity were measurable in marrow incubated in medium without cytokines, or in medium containing IL1 alone. In contrast, culture in medium containing IL2 resulted in a dose-dependent development of lytic activity. NK and LAK activities could be differentiated by the percentage of cultures in which the activity developed, the dose of IL2 required, the time kinetics of induction, and the effect of depletion of residual cells with NK phenotype prior to culture. The most lytically active effectors of both activities, however, were CD56+. Immunofluorescence analyses before and after culture with IL2 revealed that Leu19+ (CD56) cells increased from less than 2% to as much as 17% of the total marrow cells and showed the appearance of a population of CD56+CD16- cells. The addition of IL1 to the marrow cultures increased NK activity when suboptimal amounts of IL2 were used (less than or equal to 100 U/ml), but did not increase LAK activity at any concentration of IL2. A higher number of NK cells, as well as MY7+(CD13+) myeloid cells were recovered from cultures containing IL1 plus IL2, indicating that NK cells as well as myeloid cells had a growth advantage in the presence of IL1. IL2 receptor (CD25) expression was low in all cultures but was consistently higher in cultures containing IL1 and IL2, however, CD25 was not coexpressed on NK cells. These studies indicate that early NK cell precursors can grow and differentiate in response to IL2 and that NK and LAK lytic activities may be acquired at different developmental stages. IL1 may serve to promote the responsiveness of NK cell progenitors to low concentration of IL2 by a mechanism which may not require expression of CD25.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Keever CA,Pekle K,Gazzola MV,Collins NH,Gillio Adoi
10.1016/0008-8749(90)90313-gsubject
Has Abstractpub_date
1990-03-01 00:00:00pages
211-26issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(90)90313-Gjournal_volume
126pub_type
杂志文章abstract::When guinea pigs were treated by complete Freund's adjuvant (CFA), eosinophil-directed chemotactic inhibitory factor (ECIF) appeared in the serum of the treated animals. The inhibition of the response of eosinophils by ECIF was selective for the T cell-derived chemotactic factor, which has been isolated from DNP-ascar...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90202-8
更新日期:1984-11-01 00:00:00
abstract::Males exhibit stronger sympathetic nervous system (SNS) activity, but weaker primary CD4+ T-cell (auto)immune responses. To test the role of catecholamines, major end-point SNS mediators, in this dimorphism, influence of propranolol (β-adrenoceptor blocker) on mitogen/neuroantigen-stimulated CD4+ T cells from female a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.12.009
更新日期:2019-02-01 00:00:00
abstract::Gene products coded for within the HLA complex play an important role in the control of immune responses. Class I antigens, coded for by the HLA-A, B, and C loci, are expressed by virtually all mononuclear blood cells. Class II antigens, coded for by the DR, DQ, and DP loci, have a more limited tissue distribution. Th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90050-x
更新日期:1986-07-01 00:00:00
abstract::Study of primary immune responses in leprosy has been limited, since disease becomes manifest long after infection or is not detectable. To study primary immune responses, we immunized in vitro human peripheral blood mononuclear cells from unexposed individuals using phenolic glycolipid 1 (PGL-1), an important water-i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90058-j
更新日期:1991-10-01 00:00:00
abstract::Neuroendocrine hormones such as growth hormone (GH) and prolactin (PRL) have been demonstrated to accelerate the recovery of the immune response after chemotherapy and bone marrow transplantation and to enhance the restoration of immunity in individuals infected with HIV and in normal individuals with compromised immu...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2007.12.003
更新日期:2008-03-01 00:00:00
abstract::We investigated the role of HLA-DR molecules in T cell stimulation by staphylococcal enterotoxin A (SEA). Previous results with immobilized purified HLA-DR preincubated with peptide showed that peptide-specific T cell clones were able to bind to and proliferate in response to purified HLA-DR/peptide complexes in the a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1255
更新日期:1993-10-15 00:00:00
abstract::There are still questions about whether macrophage differentiation is predetermined or is induced in response to tissue microenvironments. C2D macrophage cells reside early in the macrophage lineage in vitro, but differentiate to a more mature phenotype after adoptive transfer to the peritoneal cavity (PEC-C2D). Since...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.012
更新日期:2011-01-01 00:00:00
abstract::We have addressed the differential regulatory properties that IFN-gamma and IL-4 exert on macrophage (M phi) subpopulations. For this purpose, Thyoglicolate-, Peptone-, and Con A-elicited M phi, as well as bone marrow-derived M phi and P388D1 cells, were cultured in the presence of either IFN-gamma or IL-4. The expres...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90347-e
更新日期:1991-04-15 00:00:00
abstract::Peripheral blood lymphocytes (PBL) from patients with herpes simplex virus (HSV)-1 recurrences in the cornea only (Group I) exhibited reduced lysis of HSV-1-infected targets compared to PBL from patients with oral-facial and corneal HSV recurrences (Group II). The cytotoxic lymphocytes appeared to belong to a subpopul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90305-8
更新日期:1984-02-01 00:00:00
abstract::We have investigated the ability of long-term cultured T helper (Th) cell lines to help an in vivo cytotoxic T lymphocyte (CTL) response to non-H-2 alloantigens (minor antigens). Th cell lines specific for various single or undefined minor antigens were selected by regular restimulation with antigen in vitro. They wer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90309-5
更新日期:1984-02-01 00:00:00
abstract::The expression of HLA Class II antigens by resting and phytohemagglutinin (PHA)-activated T cells and their functional properties in autologous mixed-lymphocyte reactions (MLR) were investigated in patients with chronic active hepatitis, with alcoholic cirrhosis, and with primary biliary cirrhosis. In all groups of pa...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90341-2
更新日期:1986-10-01 00:00:00
abstract::The role of the cytokines IL-1 alpha, IL-1 beta, and IL-6 and the cell adhesion molecules ICAM-1, LFA-1 (alpha and beta), and Mac-1 as accessory molecules for stimulation of T cells by the superantigen staphylococcal enterotoxin B (SEB) was examined. Both blood monocytes and alveolar macrophages were used as accessory...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90164-k
更新日期:1992-05-01 00:00:00
abstract::Mouse thyroglobulin (MTg)-sensitized spleen cells activated in vitro with MTg induced experimental autoimmune thyroiditis (EAT) in which the thyroid infiltrate consists primarily of mononuclear cells (MNC) (lymphocytic EAT). MTg-sensitized spleen cells cultured with MTg together with anti-IL2R antibody induce a granul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0208
更新日期:1996-08-25 00:00:00
abstract::Mice carrying a rearranged TCR Vbeta 8.2 transgene express the Vbeta protein on the vast majority of peripheral T-cells. The bone marrow and peripheral blood, as well as other lymphoid organs of both untreated animals and animals depleted of T-cells by neonatal thymectomy and/or injection from birth of monoclonal anti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1168
更新日期:1997-10-10 00:00:00
abstract::In this study, skin-infiltrating cells were prepared from guinea pig delayed-type hypersensitivity reaction sites, and their phenotypic properties and cytokine production were examined. Our cell preparations contained around 45% nonspecific esterase-positive cells and a few skin-derived cells. Skin-infiltrating cells ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1139
更新日期:1995-08-01 00:00:00
abstract::MHC class II-restricted antigen presentation requires trafficking of newly synthesized class II-invariant chain complexes from the trans-Golgi network to endosomal, peptide-loading compartments. This transport is mediated by dileucine-like motifs within the cytosolic tail of the invariant chain. Although these signals...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1817
更新日期:2001-06-15 00:00:00
abstract::The proliferative response of peripheral blood mononuclear cells (PBMC) in synthetic serum-free media depends on the presence of sufficient amounts of transferrin (Tf). In the present communication we show that the reduction of Tf concentration in culture media results in a decreased proliferation, whereas lymphokine ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90038-d
更新日期:1991-02-01 00:00:00
abstract::Cells from the spleen, lymph nodes, and peritoneum of DBA/2 mice bearing a subcutaneous tumor mediate nonspecific suppression of an in vitro antibody response to sheep red blood cells (SRBC) when cocultured with a normal T-cell subset(s). The spleen cells from the tumor-bearing mouse required for the suppression bear ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90047-3
更新日期:1983-07-01 00:00:00
abstract::Mice repeatedly immunized with the methanol extraction residue fraction of tubercle bacilli (MER) in incomplete Freund's adjuvant produced high titers of circulating antibodies against MER, as assessed by the enzyme-linked immunosorbent assay (ELISA) method. Spleen cells derived from these animals failed to respond to...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90021-8
更新日期:1985-05-01 00:00:00
abstract::Staphylococcal enterotoxin B (SEB) is a potent polyclonal activator of both human and murine T cells. We previously reported data which show that SEB-induced T cells suppress antibody secretion by various mouse plasmacytoma cell lines. This suppression of antibody secretion was found to be both idiotype and isotype no...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90227-i
更新日期:1990-09-01 00:00:00
abstract::The many signals that control the progress of various immune responses to both foreign and self antigens can be divided into no less than three major groups. The first group is the initial positive stimulus, associated with activation events through antigen receptors and their associated proteins. These signals launch...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/0008-8749(90)90174-p
更新日期:1990-10-01 00:00:00
abstract::Bispecific antibodies have been successfully introduced into clinical application. γδ T cells are of special interest for tumor immunotherapy, due to their recognition of pyrophosphates that are overproduced by many tumor cells resulting in HLA-nonrestricted tumor cell killing. Here we describe in detail a [(Her2)2 × ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.04.009
更新日期:2015-07-01 00:00:00
abstract::An IgG1 monoclonal antibody, termed ACM-1, has been shown to react with rabbit T cells, but not Ig+ cells or macrophages. This antibody appears to recognize the same epitope as the previously described 9AE10 antibody and, together with 9AE10, has been used to obtain highly pure and fully functional T- and B-cell popul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90244-2
更新日期:1984-05-01 00:00:00
abstract::A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1279
更新日期:1998-05-01 00:00:00
abstract::FOXP3+ regulatory T cells (Tregs) constitute a critical barrier that enforces tolerance to both the self-peptidome and the extended-self peptidome to ensure tissue-specific resistance to autoimmune, allergic, and other inflammatory disorders. Here, we review intuitive models regarding how T cell antigen receptor (TCR)...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2020.104173
更新日期:2020-09-01 00:00:00
abstract::Macrophages in varying states of activation differ in their ability to perform antibody-dependent cellular cytotoxicity (ADCC) and antibody-independent macrophage-mediated tumor cytotoxicity (MTC). To define further the activation requirements for macrophages to perform various cytolytic functions, we stimulated pepto...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90255-a
更新日期:1991-06-01 00:00:00
abstract::We investigated the influence of Panagen DNA preparations on laboratory animals and IFN-induced human dendritic cells, as well as analyzed the data from a phase II clinical trial in the therapy of breast cancer. It was shown that this treatment resulted in increased number of CD8+/perforin+ T cells in peripheral lymph...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.04.004
更新日期:2012-03-01 00:00:00
abstract::Modification of cytokine production by gender hormones has been postulated to affect disease susceptibility and outcome. Here we investigate the effect of gender and the menstrual cycle on production of cytokines. Mononuclear cells were isolated every week for 10 consecutive weeks from healthy pre-menopausal women and...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.04.013
更新日期:2008-02-01 00:00:00
abstract::Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus transformed B cells is mainly mediated by LFA-1 (CD11a/CD18) and CD2 molecules. Low-affinity binding of resting T cells can be transiently upregulated by cross-linking of CD3-TCR (T cell receptor) complexes. This inside-out signaling influences i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1211
更新日期:1993-09-01 00:00:00
abstract::Ligation of CD40 using anti-CD40 or soluble CD40-ligand activates numerous intracellular kinases which transduce signals to the nucleus. The nature whereby these signaling events are coupled to distal functional events in B cells is poorly understood. In this study, using anti-CD40 monoclonal antibodies which recogniz...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1645
更新日期:2000-05-01 00:00:00