Abstract:
:The proliferative response of peripheral blood mononuclear cells (PBMC) in synthetic serum-free media depends on the presence of sufficient amounts of transferrin (Tf). In the present communication we show that the reduction of Tf concentration in culture media results in a decreased proliferation, whereas lymphokine production and the expression of activation markers (IL-2 receptor; transferrin receptor, (TfR); HLA class II) remain unchanged. To examine whether this effect is due to iron depletion we added iron chelates (ferric citrate, FeCi; ferric nitrilotriacetic acid, FeNTA) which can be internalized by cells without the requirement for Tf. The iron chelates could fully restore the proliferative response even in complete absence of Tf, suggesting that the observed inhibitory effect was indeed caused by iron depletion. Addition of a monoclonal TfR antibody, J 64, also caused a marked inhibition of proliferation of PBMC in regular serum-containing medium as well as in Tf-free synthetic medium; this effect could not be overcome by any of the tested iron chelates. Therefore, growth inhibition caused by J 64 cannot simply be attributed to iron starvation. These data suggest that J 64 may interfere with processes others than iron uptake and that the TfR might confer a necessary promoting signal for lymphocyte proliferation.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Keyna U,Nüsslein I,Rohwer P,Kalden JR,Manger Bdoi
10.1016/0008-8749(91)90038-dsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
411-22issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(91)90038-Djournal_volume
132pub_type
杂志文章abstract::Sialoadhesin (Sn) is a surface receptor expressed on macrophages in steady state conditions, but during inflammation, Sn can be upregulated both on macrophages and on circulating monocytes. It was shown for different species that Sn becomes internalized after binding with monoclonal antibodies. These features suggest ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.11.009
更新日期:2017-02-01 00:00:00
abstract::Transformed murine fetal thymocyte cell lines were derived by incubating fetal thymic organ cultures with a v-myc/v-raf-containing retroviral construct in order to model developmental stages within the early triple negative (CD3-CD4-CD8-) thymocyte population. The resulting 10 cell lines had a lymphoid morphology, wer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0170
更新日期:1996-07-10 00:00:00
abstract::Rotavirus infection of permissive cells is a multi-step process that requires interaction with several cell surface receptors. Integrins alpha2beta1, alpha4beta1, alphaXbeta2, and alphavbeta3 are involved in the attachment and entry into permissive cells for many rotavirus strains. However, possible roles of known par...
journal_title:Cellular immunology
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更新日期:2005-07-01 00:00:00
abstract::Altered neutrophil function may contribute to the development of AIDS during the course of HIV infection. It has been described that Nef, a regulatory protein from HIV, can modulate superoxide production in other cells, therefore altered superoxide production in neutrophils from HIV infected patients, could be seconda...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.03.012
更新日期:2010-01-01 00:00:00
abstract::The precise role of the granular enzyme A (granzyme A), a serine protease, in the lytic process of cytotoxic T lymphocytes (CTL) is not clear. We have recently constructed a CTL line transfected with the antisense gene of granzyme A (a-GrA). These a-GrA CTL had lower GrA activity as well as decreased lytic activities,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0300
更新日期:1996-12-15 00:00:00
abstract::OVA-specific T cells were immortalized by infection with radiation leukemia virus (RadLV). Some clones derived from such population were shown to exhibit helper activity. We then tested clones without such function and found among them some that secreted macrophage migration inhibition factor (MIF) and leukocyte migra...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90328-x
更新日期:1986-10-01 00:00:00
abstract::To investigate the consequences of food allergy in adipose tissue and metabolism, we used a murine model in which mice have been sensitized subcutaneously with ovalbumin and further received antigen-containing diet. Allergic mice presented a significant weight loss 7 days after oral challenge with a concomitant decrea...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.05.008
更新日期:2011-01-01 00:00:00
abstract::We have performed immunoperoxidase staining on cryostat tissue sections and immunofluorescence analysis on cell suspensions to identify cells expressing the alpha/beta T cell antigen receptor during ontogeny and adult life in chickens. We used the mouse monoclonal antibody, TCR2, which was previously shown to recogniz...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90079-7
更新日期:1990-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1058
更新日期:1993-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90243-2
更新日期:1988-10-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1202
更新日期:1995-10-15 00:00:00
abstract::The cellular requirements for stimulating primed alloreactive T cells have been investigated. In vitro-primed secondary alloreactive cells, long-term lines, and Ly 1+2- noncytolytic clones which reacted with allo-H-2K, D, or Mls (M locus) antigens were tested. The data indicated that a specialized antigen-presenting c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90032-2
更新日期:1985-03-01 00:00:00
abstract::We have previously shown that whole cell preparations of Candida albicans are capable of inducing immunosuppressive B-cell activity both in vivo and in vitro. In an effort to characterize the components of the yeast which manifest this immunomodulatory activity, we have successfully generated a soluble extract with di...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90149-4
更新日期:1989-08-01 00:00:00
abstract::In view of the critical role played by bacillus Calmette-Guérin (BCG) in the development and functional activation of protective T cells against tuberculosis, it has become important to understand the mechanisms by which cytokines regulate BCG-mediated immune responses. There is evidence that cytokine-mediated suppres...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1662
更新日期:2000-06-15 00:00:00
abstract::The susceptibility of murine embryonic cells to CTL lysis has been assessed. Zona-free blastocysts were not lysed during 6 hr incubation with CTL sensitized against the embryonic genotype and remained capable of implantation and normal development in vivo as well as outgrowth in vitro. Freshly dissociated cells from p...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90037-2
更新日期:1987-02-01 00:00:00
abstract::Observational studies in multiple sclerosis (MS) demonstrated altered expression of chemokine receptors (CkRs) on comparable populations of mononuclear cells (e.g. CD4(+)/CD45RO(+) T-cells) in brain sections compared with blood. These findings raised questions about the regulation of CkRs on trafficking cells. Regulat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.12.001
更新日期:2006-09-01 00:00:00
abstract::Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca(2+)-dependent activation pathway. In the present experiments we studied the pr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.01.004
更新日期:2013-01-01 00:00:00
abstract::Cytotoxic T-cell (CTL) cultures were generated from five ovarian cancer patients (OvCTL) and from three breast cancer patients (BrCTL). All CTL lines were T-cell receptor (TcR) alphabeta+ and predominantly CD8+ (73 +/- 13%). These CTL lines preferentially recognized autologous tumor cells in an HLA class I-restricted,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.1055
更新日期:1997-02-01 00:00:00
abstract::The pathogenic antibody response to acetylcholine receptor (AChR) in experimental autoimmune myasthenia gravis (EAMG) is T cell dependent. Therefore, it should be possible to design specific immunotherapeutic approaches to treat EAMG (and human MG) by interfering with AChR-specific helper T cells. Productive T cell ac...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.0012
更新日期:1995-11-01 00:00:00
abstract::We previously reported the amino acid sequence of the human macrophage chemotactic factor with a molecular weight of 1000 (MCF-1) was WLGREDGSE. In this study we examined the roles of MCF-1 and tumor necrosis factor (TNF) in a guinea pig delayed-type hypersensitivity (DTH) reaction. When macrophage chemotactic activit...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1038
更新日期:1995-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.06.003
更新日期:2006-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90298-6
更新日期:1990-03-01 00:00:00
abstract::Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (alpha 1 and alpha 2) of H-2Kb and the carboxy-terminal domains (alpha 3, transmembrane, and ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90235-2
更新日期:1986-04-15 00:00:00
abstract::In the present work we have studied the occurrence and the characteristics of human B lymphocytes which are capable of spontaneous immunoglobulin (Ig) production in short-term cultures. It was found that this type of B cells secreted predominantly IgG and were present in the circulation as well as in the lymphoid tiss...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90280-7
更新日期:1986-03-01 00:00:00
abstract::Estrogen displays potent immunoregulatory properties. In the present study we investigated the in vivo effect of estrogen on natural killer (NK) cell cytotoxic activity in several mouse strains. After castration, 17 beta-estradiol was administered in low or high doses. After 3-6 weeks of hormone administration an in v...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1262
更新日期:1994-10-01 00:00:00
abstract::Activated macrophages synthesize and release numerous tumoricidal soluble factors that can be divided into receptor- or nonreceptor-dependent agents. Tumor necrosis factor (TNF) would be an example of the former. In our experimental model the killing of EL4 thymoma cells by syngeneic activated macrophages involves, bu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90116-9
更新日期:1990-04-15 00:00:00
abstract::Microglia are important immunoregulatory cells within the central nervous system (CNS). Viral infection of primary microglia and splenic macrophage clones revealed that both exhibited a heterogeneous, but relatively low, sensitivity to cytolysis mediated by CD8(+) cytotoxic T lymphocytes (CTL). The majority of clones ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1581
更新日期:1999-11-25 00:00:00
abstract:BACKGROUND:Interleukin (IL)-23, composed of p19 and p40 subunits, has diverse functions in regulating immune systems, enhancing cell-mediated immunity. In the present study, we investigated whether forced expression of the p19-linked p40 gene in murine mammary cancer cells (MA891) produced antitumor effects in vivo. Tu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.05.002
更新日期:2009-01-01 00:00:00
abstract::As fascinating a molecule as it can potentially get, nuclear factor-κB (NF-κB), a regulatory transcription factor, is as intriguing. NF-κB is a dimeric complex that controls the transcription of essential genes. NF-κB is involved in a variety of responses that play a pivotal role in regulating the immune response to i...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2011.06.021
更新日期:2011-01-01 00:00:00
abstract::The mechanism by which purinergic agonists modulate murine T-lymphocyte activation and proliferation was investigated. Adenosine and other compounds such as ATP and 2-chloroadenosine (ClAdo) were found to block T-cell mitogenesis induced by concanavalin A (Con A) in a dose-dependent fashion. The nonmetabolizable adeno...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90199-1
更新日期:1986-08-01 00:00:00