Abstract:
:Cytotoxic T-cell (CTL) cultures were generated from five ovarian cancer patients (OvCTL) and from three breast cancer patients (BrCTL). All CTL lines were T-cell receptor (TcR) alphabeta+ and predominantly CD8+ (73 +/- 13%). These CTL lines preferentially recognized autologous tumor cells in an HLA class I-restricted, and in part HLA-A2-restricted, manner. In addition, the CTL lines recognized allogeneic HLA-A2+ ovarian and breast tumor cells. Specific recognition was determined by T-cell-mediated cytotoxicity as well as cytokine release. Coculture of irradiated autologous tumor cells with OvCTL induced secretion of IFN-gamma, GM-CSF and TNF-alpha, but not IL-4, indicating a T helper-1-type response. Similar results were obtained when OvCTL and BrCTL were stimulated with histologically matched HLA-A2+ tumor cells. Also, BrCTL stimulated with HLA-A2+ but not HLA-A2- ovarian tumor cells produced significant levels of GM-CSF and TNF-alpha. Finally, the Her2/neu peptide p654-662, earlier identified as a tumor antigen in both ovarian and breast cancer, induced cytotoxicity as well as the specific release of IFN-gamma and TNF-alpha but not IL-4 by OvCTL and BrCTL. Thus, tumor-specific recognition by CTL was verified by cytotoxicity and cytokine release. The secretion of Th1-like cytokines as opposed to Th2-like cytokines suggest that therapeutically OvCTL and BrCTL could potentially enhance the endogenous immune response to tumor.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Goedegebuure PS,Douville CC,Doherty JM,Linehan DC,Lee KY,Ganguly EK,Eberlein TJdoi
10.1006/cimm.1996.1055subject
Has Abstractpub_date
1997-02-01 00:00:00pages
150-6issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(96)91055-2journal_volume
175pub_type
杂志文章abstract::Mycobacterial antigens including BCG stimulate human peripheral blood mononuclear cells resulting in cellular proliferation and the release of inflammatory cytokines such as TNF-alpha. However, the signal transduction mechanisms responsible for the BCG-induced cell activation are not completely understood. In this stu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0185
更新日期:1996-07-10 00:00:00
abstract::To characterize the binding properties of the Fc receptors on human macrophages, the binding of radiolabeled human IgG1 to peritoneal macrophages was assessed. Cells were obtained at the time of diagnostic laparoscopy from women undergoing evaluation of infertility. Macrophages bound on the average more IgG1 monomer t...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90403-9
更新日期:1984-07-01 00:00:00
abstract::G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T lymphocytes. Given that mature T cells which lack antigen receptors (CDl-Ti) are refractory to stimulation through CD2 or other accessory molecules, T cell rece...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90050-2
更新日期:1990-07-01 00:00:00
abstract::In test in vitro, four sulfated lycium barbarum polysaccharides (sLBPSs) with different degrees of sulfation (DS), sLBPS0.7, sLBPS1.1, sLBPS1.5 and sLBPS1.9, were added into cultured chicken peripheral lymphocytes and the changes of lymphocytes proliferation were compared by MTT assay taking the non-modified LBPS as c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.04.001
更新日期:2010-01-01 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a curable strategy for the treatment of hematological malignancies and nonmalignant diseases. However, graft-versus-host disease (GVHD) and relapse are still two major causes of morbidity and mortality after allo-HSCT, and both restrict the improvement ...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2019.103991
更新日期:2019-12-01 00:00:00
abstract::It is still a matter of debate, whether tolerance toward self-non-MHC antigens is due to intrathymic deletion or to regulatory processes in the periphery. To further pursue this question, responsiveness toward TNP and an anti-TNP monoclonal antibody (Sp6) carrying a recurrent idiotype was evaluated in prenatally trini...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90298-6
更新日期:1990-03-01 00:00:00
abstract::The adoptive transfer of tumor-reactive T lymphocytes has recently been demonstrated to be an effective means for mediating the regression of experimental intracranial fibrosarcomas. In this study, mice bearing syngeneic intracranial GL261 gliomas were cured by the combination of sublethal whole body irradiation follo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1140
更新日期:1997-06-15 00:00:00
abstract::This study examined the characteristics of functional anergy of natural killer cells (NK) following their interaction with target cells. Purified NK cells were cocultured with K562 for 15 min or 4 hr to allow for binding of targets to NK cells. The resulting NK-target conjugates were then dissociated by EDTA, and the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(95)80013-9
更新日期:1995-01-01 00:00:00
abstract::The relay hypothesis [R. Nayak, S. Mitra-Kaushik, M.S. Shaila, Perpetuation of immunological memory: a relay hypothesis, Immunology 102 (2001) 387-395] was earlier proposed to explain perpetuation of immunological memory without requiring long lived memory cells or persisting antigen. This hypothesis envisaged cycles ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.05.001
更新日期:2007-03-01 00:00:00
abstract::CD48, the murine homolog of human CD58, binds to CD2 in rats and mice. Whereas inhibition of CD2 signaling leads to profound immunosuppression, no information is available on CD48-targeted therapy in the rat. We could show that anti-CD2 treatment (OX34) efficiently inhibited TCR-driven as well as CD2-mediated prolifer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1204
更新日期:1997-11-25 00:00:00
abstract::We and other investigators have previously demonstrated that mycoplasmas induce macrophage-mediated lysis of tumor cells, but the mechanism responsible for this process had, thus far, not been clarified. We now report that addition of either viable or heat-killed Mycoplasma orale to murine macrophages induces a cytoly...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90012-9
更新日期:1989-06-01 00:00:00
abstract::The mechanism of human peripheral blood monocyte-mediated cytotoxicity was investigated using the HT-29 human colon adenocarcinoma line, A673 human rhabdomyosarcoma line, and A375 human melanoma line as target cells. Pretreatment of these target cells with 100 U/ml of recombinant human interferon (IFN)-gamma for 48 hr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90236-4
更新日期:1986-04-15 00:00:00
abstract::B-1a cells represent a distinct B cell population with unique phenotype, self-renewing capacity and restricted Igμ repertoire. They primarily locate in body cavity and also exist in spleen. The different subpopulations of B-1a cells are heavily affected by local environment. Our previous studies revealed that MARVEL-d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104100
更新日期:2020-06-01 00:00:00
abstract::Previous studies have shown that the anti-CD4 mAb W3/25 strongly enhances T cell APC (T-APC) activity. In this study, single positive CD4+ and double negative (DN) (CD4-CD8-) T-helper cells specific for the 55-69 or 72-86 sequence of guinea pig (GP) myelin basic protein (GPMBP) were used to study CD4 regulation of T-A...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1299
更新日期:1998-06-15 00:00:00
abstract:BACKGROUND:Piper nigrum L. (Piperaceae) is commonly used as a spice and traditional medicine in many countries. It has been reported to have anti-oxidant, anti-bacterial, anti-tumor, anti-mutagenic, anti-diabetic, and anti-inflammatory properties. However, the protective role of P. nigrum on epithelial function of uppe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.104035
更新日期:2020-05-01 00:00:00
abstract::Castration of normal male mice leads to splenic enlargement and expansion of the B cell population. Since the spleen does not express receptors for androgens, these changes are most likely mediated by effects of androgens on other target organs. Two potential sites of androgen-mediated effects on B cells are evaluated...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1227
更新日期:1997-12-15 00:00:00
abstract::The kinetics and quality of the alloimmune reaction were studied in CBA (H-2k) mice treated for passive enhancement of tumor allografts (Sa 1 indigenous of A/J (H-2a or H-2k/d) mice). Serum samples of treated animals were tested for their biological properties relevant to different antibody isotypes in vitro (hemagglu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90306-5
更新日期:1985-10-01 00:00:00
abstract::The effects of oral administration of ovalbumin (OVA) on acute OVA-induced arthritis (OIA) in rats, which is mediated by Arthus reaction to the antigen in the joint space, were investigated. The oral administration of OVA before immunization with OVA significantly suppressed the development of acute OIA in a dose-depe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.1049
更新日期:1997-01-10 00:00:00
abstract::We have reported that Burkitt lymphomas (BL) that arise in HLA-A11 positive individuals are resistant to lysis by HLA-A11-specific and HLA-A11-restricted CTLs(10,11). Here we show that this phenomenon can be explained by a selective loss of the HLA-A11 polypeptide. The HLA-A11 negative phenotype is due to a regulatory...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90207-4
更新日期:1989-05-01 00:00:00
abstract::During the gestational cycle the placental tissue does not express class II MHC antigens and whether this phenomenon is important to fetal survival has not yet been evoked. It has been reported that class II antigen expression precedes renal and cardiac graft rejection, which may also be the case in fetal abortion. In...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90039-t
更新日期:1990-07-01 00:00:00
abstract::The NK-susceptibility of dystrophic mouse myoblast cells was investigated. Spleen cells from 8- to 10-week-old normal (+/+) and dystrophic (dy2J/dy2J) male C57BL/6J mice were fractionated on Percoll density gradients and the cells at each density interface were incubated with either 51Cr-labeled YAC-1 or myoblast cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90003-1
更新日期:1986-06-01 00:00:00
abstract::Anti-TNF-α therapies have been applied in RA treatment, but the regulatory effect of the drug on immune system is not clear. In this study, we included 33 active RA patients and divided them into two groups. One group received anti-TNF-α mAb+methotrexate for 24 weeks, the other group got placebo+methotrexate for the f...
journal_title:Cellular immunology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.cellimm.2012.09.001
更新日期:2012-09-01 00:00:00
abstract::The purpose of the present study was to compare the phenotype of tissue macrophages with that of their precursors in the bone marrow and blood. The phenotype was determined on the basis of the quantitative binding of monoclonal antibodies to cell-surface antigens (antigen F4/80, complement receptor III, Fc receptor II...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90085-2
更新日期:1987-04-01 00:00:00
abstract::Recently, it has been reported that IL-4 production by murine Th2 cell lines is insensitive to inhibition by E-type prostaglandins. In the present study, IL-4 production in vitro by freshly isolated concanavalin A (Con A)-stimulated murine spleen cells was readily suppressed by PGE2 with an I50 of 2 nM. Comparable sup...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(95)80039-l
更新日期:1995-02-01 00:00:00
abstract::The function of interleukin-1 alpha and interleukin-1 beta (IL-1 alpha, IL-1 beta) in tetanus toxoid (TT) induced T-cell proliferation in cultures of peripheral blood mononuclear cells (PBL) obtained from healthy donors was assessed by using neutralizing antisera to IL-1 alpha and IL-1 beta. The neutralizing capacity ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90149-6
更新日期:1991-11-01 00:00:00
abstract::Twenty-seven different CD45 monoclonal antibodies (mAb) were assessed for their ability to block cytotoxicity of alloreactive CD4+ MHC class II-specific or CD8+ class I-specific human T cell clones (n = 3 and 5, respectively). Twelve of 27 blocked the former but only 1/27 the latter, although all 27 significantly inhi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90151-g
更新日期:1990-05-01 00:00:00
abstract::Pentraxin 3 (PTX3) is a prototype protein of long pentraxin. PTX3 is produced by various cells, such as monocytes/macrophages (Mphis) in response to lipopolysaccharide (LPS) and proinflammatory signals. We performed immunoblotting, immunohistochemical staining, reverse transcriptase-polymerase chain reaction (RT-PCR),...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.09.003
更新日期:2007-08-01 00:00:00
abstract::Stimulated macrophages are known to inhibit the growth of certain tumor cells. Using mouse peritoneal exudates as a source of macrophages and the mastocytoma cell line P815 as the target, the inhibition was found to depend on direct contact between the macrophages and the growing cells. Cytostatic activities were dete...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90395-3
更新日期:1986-02-01 00:00:00
abstract::The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.03.023
更新日期:2011-01-01 00:00:00
abstract::The nuclear receptor Small Heterodimer Partner (SHP) is a transcriptional target and inhibitor of Liver Receptor Homolog 1 (LRH-1), the transcriptional regulator of intestinal glucocorticoid (GC) synthesis. The role of SHP in the regulation of intestinal GC synthesis and its impact on T cell-mediated anti-viral immune...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.08.009
更新日期:2018-12-01 00:00:00